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Gene information
General information
TC005900 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 5526896..5528093
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005900
    iBeetle screen iB_00957
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005900 RA
      MGKPSFGDL KTDKGVKEL NSFLESRSY 
      IEGYSLSQA DLDAFGKLQ KAPAANLPH 
      ALRWYNHVK SFTPAELKS LGVSGACPM 
      KTAAPTTKD DDDDVDLFA SDEEEDAEA 
      ARIREERLK AYAEKKSKK PELIAKSSI 
      VLDVKPWDD ETDMKEMEK NVRTIEMDG 
      LVWGASKLV PVGYGINKL QIMCVVEDL 
      KVSVDELVE KIQEFEDFV QSVDIAAFN 
      KI
    CDS sequence
    • >TC005900 RA
      atgggcaaa cccagcttt ggcgacctc aaaaccgac 
      aaaggtgtg aaggaactg aacagtttc ctcgaaagt 
      cgcagctac atcgaaggc tactcgttg tctcaggcc 
      gacttggac gcgtttggc aagctccaa aaggcccca 
      gctgcgaat ttgccccac gctttgcgt tggtacaac 
      catgtcaag tctttcact cctgcggag ctgaagtcg 
      ttgggggtt tcgggggca tgccccatg aaaactgca 
      gctccgacc accaaagat gatgatgat gatgtcgat 
      ttgttcgcg tctgacgag gaagaggat gcggaggct 
      gccagaata agggaggag cgtctgaag gcctacgct 
      gagaagaag tcaaagaaa ccggaactt attgccaag 
      tcaagtatt gtgcttgat gtcaagccc tgggatgat 
      gagaccgat atgaaggag atggagaag aatgtcagg 
      actattgaa atggatggg ctggtttgg ggggcctcc 
      aagttggtt cctgtgggc tatggaatc aacaagttg 
      caaattatg tgcgtggtt gaagacttg aaggtgtct 
      gttgatgag ttggtcgag aagattcag gagtttgag 
      gatttcgtc cagtctgtc gacattgca gcctttaat 
      aagatttaa
    mRNA sequence
    • >TC005900 RA
      aacgtgcca atttcctta aattacctt atttttgtg 
      ttttcaata acaaacgtc aaaaaatga cgtccatta 
      ctaaagttg gtggcactg aactaacct aaaaaactc 
      tctttccgt taccatctg tcactggcg tggtcactc 
      tcacatttg cactcaaaa aatgggcaa acccagctt 
      tggcgacct caaaaccga caaaggtgt gaaggaact 
      gaacagttt cctcgaaag tcgcagcta catcgaagg 
      ctactcgtt gtctcaggc cgacttgga cgcgtttgg 
      caagctcca aaaggcccc agctgcgaa tttgcccca 
      cgctttgcg ttggtacaa ccatgtcaa gtctttcac 
      tcctgcgga gctgaagtc gttgggggt ttcgggggc 
      atgccccat gaaaactgc agctccgac caccaaaga 
      tgatgatga tgatgtcga tttgttcgc gtctgacga 
      ggaagagga tgcggaggc tgccagaat aagggagga 
      gcgtctgaa ggcctacgc tgagaagaa gtcaaagaa 
      accggaact tattgccaa gtcaagtat tgtgcttga 
      tgtcaagcc ctgggatga tgagaccga tatgaagga 
      gatggagaa gaatgtcag gactattga aatggatgg 
      gctggtttg gggggcctc caagttggt tcctgtggg 
      ctatggaat caacaagtt gcaaattat gtgcgtggt 
      tgaagactt gaaggtgtc tgttgatga gttggtcga 
      gaagattca ggagtttga ggatttcgt ccagtctgt 
      cgacattgc agcctttaa taagattta aaaatgtaa 
      tttttcaag atgaataaa tgcgtttat tgatgagtt 
      tggattaat ttataaagg cgcaaatta ctaaacccg 
      gattgtgat tgtgatgat tgagagtca agtctggta 
      tttggttgg taataaaat tctcttggt tctgggggt 
      ttcacatag ttggataaa accggttct ttgtctttt 
      tggggcaaa ggttccctc gatgttatg accggtggt 
      gatttagag ggcacctct agtggtatc aaatcttta 
      taaattggt ttgtctaag ggaaaattc acttttcga 
      gtcacctac aaagtagaa tgttacttc atgaaaata