General information | |||
TC005895 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 5900368..5915555 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC005895 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00956 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
- >TC005895 RA
MAMQREVGV GDFVLLDKI DLGNFMKNL ELRFKHGKI YTYIGEVCV SVNPYKTMN IYDQKFIDQ YKGRELFEN PPHIFAIAD ASHKVMQQQ GKDTCIVIS GESGAGKTE ASKIIMKYI AAVTNKDGQ KEIERVKNV LLQSNSILE AFGNAKTNR NDNSSRFGK YMDIHFDFK GDPIGGHIN KYLLEKSRV IYQQAGERN FHSFYQVLA GAGDDLLRK LHLSRNSDD YFYVKQGKA AKVDTINDR NDFRDVNSS LNTLQFSKE AQETLWRVV AAILHLGNL EFVTDGDNK LQIKNKKSL EIISELLQV QKNELNSAL CERVIAARG DIMRKEHTE AEALYGRDA LAKAIYERL FGWIVEKIN SAIAIDEKN YSKNYKSSL IGVLDIYGF EIFDNNSFE QFCINYCNE KLQQLFIEL VLKQEQEEY NREGIEWQN IDFFNNQII CELVEAPHK GILAIMDDA SKMTAEKVT DELLLETMD KQLKNHKHY TSRQLKPAD KNMRHKIDF RITHYAGDV TYCVTGFLD KNKDTLFQD FKRLMFNSN DSNLKEMFP EGSQHISQT TKRPPTAGS LFKTSMQAL VQNLYSKEP HYVRCIKPN ELKSPSAFD EERVKHQVS YLGLVENVR VRRAGFAYR QRYDLFLKR YKMISQFTW PNFRSGTDK DGVCVIMDE RKFSDDVKY GKTKIFIRS PKTLFALEA ARNDLIPGI VTLIQKTWR GYVARQQYK KMRALMTMI QAYRLKKMR VYVNALQAK FANAKRMKD FGKSVMWPA PPVSLQKTT RTLKQIYNR WRAFMILNK IPRAEWPEM KIKVAAASA LLNKRPTYG LNRRWEGNY LATHSENNN YTTFNDAVN NLKNGQHFG KVLFSSFVT KFNKFNKCA ERVILVTDL AIFKLDSLK FRNMKEGTA VKDLTGISV SPGQDQLIV LHCLGGNDL VVSLHSMKQ EDRIGELVG IVSNTYFKF KGTDLPVTV SKTITCSLG GKKRVITIN VTSEVQTPT FKKDGNNIA YLLPTSFAV VENGRDFIK H
- >TC005895 RA
atggcaatg caacgagaa gtgggcgtg ggcgacttc gtccttttg gacaagatc gatttgggc aactttatg aaaaatcta gagctcaga ttcaaacat ggcaagatt tacacctac attggcgag gtatgcgtt tcggtaaat ccctacaaa acgatgaat atctacgac caaaaattc atcgatcaa tataaaggt cgcgagctg ttcgaaaac cctcctcac atcttcgcc atcgccgac gcctctcac aaagtcatg cagcagcaa ggcaaggac acttgtatc gtaatcagc ggcgaatca ggagccgga aaaacagaa gcctcgaaa ataataatg aaatatatc gcggccgtg acgaacaaa gacggccag aaggagatc gaacgcgtg aagaacgtc cttttgcaa tcaaactcg attctagaa gcgttcgga aacgcaaaa accaaccgc aacgacaat tcctcgcgt tttgggaag tacatggac atccatttc gatttcaaa ggcgacccg atcggcgga cacatcaac aagtatctg ctcgaaaaa tcccgagtg atttaccag caagccggc gagaggaac ttccacagt ttctaccag gttttggcg ggcgctggc gacgatctt ttgaggaaa ctgcacttg agtcgcaac agtgatgac tatttttac gtcaaacag gggaaagcc gccaaagtc gacacgatc aatgaccga aatgacttc cgggatgtg aattcgtca ctgaacacg ttgcagttc tcgaaggaa gcgcaagaa actctatgg agggttgtg gcagcgatt ttgcatctt ggaaatttg gaatttgtt acagacggt gacaataaa ttgcaaatt aagaacaag aaatcgctc gaaataatt tcggagctg cttcaagtg cagaaaaac gagctaaat tcggcgctt tgtgagcgc gtgattgcc gccagaggc gacattatg aggaaggaa cacaccgaa gctgaggcg ctttacgga cgcgatgcc ctagctaag gccatttac gagcgactt ttcggttgg attgtcgag aaaatcaac tcggcgatc gccatcgac gagaaaaac tattcgaaa aactacaaa agctcgctg attggagtg ttggacatc tacgggttt gagattttc gacaacaac agtttcgag cagttctgt atcaactac tgtaacgag aagttgcag cagttgttt attgaactt gtcctgaag caggagcaa gaggagtat aatcgcgaa ggcatcgag tggcaaaac atcgatttc ttcaacaat cagatcatt tgtgagctt gttgaagcg ccacataag gggattttg gcgattatg gatgatgcg tcgaaaatg actgccgag aaagttact gatgagctt ttactagaa acgatggat aagcagttg aaaaatcat aagcattat acgtcgaga caactcaaa ccggctgat aaaaatatg aggcataag atcgatttt cggataaca cattatgcg ggtgatgtc acctattgt gtaacggga tttttggat aagaataag gatacgctc ttccaggat tttaaacgg ttaatgttt aattccaac gattcgaat ttgaaggag atgttccca gaaggctcg caacacatt tcacagacc accaaacgc cccccaacg gccggctcc ctgttcaaa acctccatg caagccctc gtccagaac ctctacagc aaagagccg cactacgtc cgctgcatc aaacccaac gaactgaaa tccccgtcg gccttcgac gaagaacgc gtcaagcac caggtcagc tacttgggt ctggtggaa aacgtgcgc gtccgtcgc gctggtttc gcctaccgt cagcgttac gacctcttc ctcaaacgc tacaaaatg atctcacag ttcacgtgg cccaacttc cgttccggc accgataaa gacggcgtt tgcgtcata atggacgaa cgcaaattc agcgatgac gtaaaatac ggcaaaacg aaaatattc attcgctcg ccgaaaacg ctattcgca ctggaagcg gcgcgaaat gacctcatc cccggcatt gtgactttg attcagaaa acgtggcgc ggctacgtc gccaggcaa cagtacaag aagatgagg gccttaatg acgatgatt caagcgtat aggttgaag aaaatgcgc gtttatgtg aacgcactc caggcgaag ttcgcaaac gcgaaacga atgaaagac ttcgggaag agcgtcatg tggccagcg ccgcccgtg tcgctgcag aagacaaca cgcactttg aagcagatt tacaatcgc tggagggcc ttcatgata cttaataaa atcccgagg gccgagtgg cccgaaatg aaaatcaag gtggccgcg gccagtgcc ttactcaac aaacggccg acttacgga ctcaacaga aggtgggag ggtaattat cttgccacg cattctgaa aataacaat tacacgacg ttcaacgat gcggttaat aacttgaaa aatggccaa cactttgga aaagtgctg ttttcgtcg ttcgttacg aaattcaac aagtttaat aagtgtgca gagcgagtg attctcgtt actgatctg gcgattttc aagcttgat tcgttgaag ttcaggaat atgaaagaa gggacggct gttaaggac ctgacaggg attagtgtc agtccggga caggatcaa ttgatcgtt ttacattgt ttgggtggg aatgattta gttgtgtcg ttgcattcg atgaagcaa gaggatcgg attggggaa ttggttggg attgtctcc aacacatat ttcaagttc aagggaacg gatcttccg gtcaccgtc agcaaaacc attacctgt tcactgggt gggaaaaag cgtgtaatt acaatcaac gtgacttcc gaggtacaa acgccaacg ttcaagaag gacggaaat aacattgcg tatttgtta ccgacaagt tttgccgtt gtggaaaac ggtcgtgat tttatcaag cattaa
- >TC005895 RA
atcggcagt caaacaacg gccggtgtg agaatgaga acatttcaa acaaacgaa cagttttat taaaattga aatggcaat gcaacgaga agtgggcgt gggcgactt cgtcctttt ggacaagat cgatttggg caactttat gaaaaatct agagctcag attcaaaca tggcaagat ttacaccta cattggcga ggtatgcgt ttcggtaaa tccctacaa aacgatgaa tatctacga ccaaaaatt catcgatca atataaagg tcgcgagct gttcgaaaa ccctcctca catcttcgc catcgccga cgcctctca caaagtcat gcagcagca aggcaagga cacttgtat cgtaatcag cggcgaatc aggagccgg aaaaacaga agcctcgaa aataataat gaaatatat cgcggccgt gacgaacaa agacggcca gaaggagat cgaacgcgt gaagaacgt ccttttgca atcaaactc gattctaga agcgttcgg aaacgcaaa aaccaaccg caacgacaa ttcctcgcg ttttgggaa gtacatgga catccattt cgatttcaa aggcgaccc gatcggcgg acacatcaa caagtatct gctcgaaaa atcccgagt gatttacca gcaagccgg cgagaggaa cttccacag tttctacca ggttttggc gggcgctgg cgacgatct tttgaggaa actgcactt gagtcgcaa cagtgatga ctattttta cgtcaaaca ggggaaagc cgccaaagt cgacacgat caatgaccg aaatgactt ccgggatgt gaattcgtc actgaacac gttgcagtt ctcgaagga agcgcaaga aactctatg gagggttgt ggcagcgat tttgcatct tggaaattt ggaatttgt tacagacgg tgacaataa attgcaaat taagaacaa gaaatcgct cgaaataat ttcggagct gcttcaagt gcagaaaaa cgagctaaa ttcggcgct ttgtgagcg cgtgattgc cgccagagg cgacattat gaggaagga acacaccga agctgaggc gctttacgg acgcgatgc cctagctaa ggccattta cgagcgact tttcggttg gattgtcga gaaaatcaa ctcggcgat cgccatcga cgagaaaaa ctattcgaa aaactacaa aagctcgct gattggagt gttggacat ctacgggtt tgagatttt cgacaacaa cagtttcga gcagttctg tatcaacta ctgtaacga gaagttgca gcagttgtt tattgaact tgtcctgaa gcaggagca agaggagta taatcgcga aggcatcga gtggcaaaa catcgattt cttcaacaa tcagatcat ttgtgagct tgttgaagc gccacataa ggggatttt ggcgattat ggatgatgc gtcgaaaat gactgccga gaaagttac tgatgagct tttactaga aacgatgga taagcagtt gaaaaatca taagcatta tacgtcgag acaactcaa accggctga taaaaatat gaggcataa gatcgattt tcggataac acattatgc gggtgatgt cacctattg tgtaacggg atttttgga taagaataa ggatacgct cttccagga ttttaaacg gttaatgtt taattccaa cgattcgaa tttgaagga gatgttccc agaaggctc gcaacacat ttcacagac caccaaacg ccccccaac ggccggctc cctgttcaa aacctccat gcaagccct cgtccagaa cctctacag caaagagcc gcactacgt ccgctgcat caaacccaa cgaactgaa atccccgtc ggccttcga cgaagaacg cgtcaagca ccaggtcag ctacttggg tctggtgga aaacgtgcg cgtccgtcg cgctggttt cgcctaccg tcagcgtta cgacctctt cctcaaacg ctacaaaat gatctcaca gttcacgtg gcccaactt ccgttccgg caccgataa agacggcgt ttgcgtcat aatggacga acgcaaatt cagcgatga cgtaaaata cggcaaaac gaaaatatt cattcgctc gccgaaaac gctattcgc actggaagc ggcgcgaaa tgacctcat ccccggcat tgtgacttt gattcagaa aacgtggcg cggctacgt cgccaggca acagtacaa gaagatgag ggccttaat gacgatgat tcaagcgta taggttgaa gaaaatgcg cgtttatgt gaacgcact ccaggcgaa gttcgcaaa cgcgaaacg aatgaaaga cttcgggaa gagcgtcat gtggccagc gccgcccgt gtcgctgca gaagacaac acgcacttt gaagcagat ttacaatcg ctggagggc cttcatgat acttaataa aatcccgag ggccgagtg gcccgaaat gaaaatcaa ggtggccgc ggccagtgc cttactcaa caaacggcc gacttacgg actcaacag aaggtggga gggtaatta tcttgccac gcattctga aaataacaa ttacacgac gttcaacga tgcggttaa taacttgaa aaatggcca acactttgg aaaagtgct gttttcgtc gttcgttac gaaattcaa caagtttaa taagtgtgc agagcgagt gattctcgt tactgatct ggcgatttt caagcttga ttcgttgaa gttcaggaa tatgaaaga agggacggc tgttaagga cctgacagg gattagtgt cagtccggg acaggatca attgatcgt tttacattg tttgggtgg gaatgattt agttgtgtc gttgcattc gatgaagca agaggatcg gattgggga attggttgg gattgtctc caacacata tttcaagtt caagggaac ggatcttcc ggtcaccgt cagcaaaac cattacctg ttcactggg tgggaaaaa gcgtgtaat tacaatcaa cgtgacttc cgaggtaca aacgccaac gttcaagaa ggacggaaa taacattgc gtatttgtt accgacaag ttttgccgt tgtggaaaa cggtcgtga ttttatcaa gcattaatt cgtgccttt tttacaaga tggttcaga tttatatgt atacaaaca acgaatttg ttgcttttt atgtactta aatgtgcga tctttgaga tcatatgtg ttgtcatca gtttgaaat tttatttta aatgtcgtg ttacgtagt tttatgaat ttatatatc gttgttaat cattccacg atggcattt tgttgattc attattttc tactaaaag tattcgcat ctggtatta tgtaaaagt gattgttgt atttatttg tatatacaa gtgccattt tttgtttat atttttgta tcgattttg gatacatag tcaagctaa agtacgtgc cttaataaa aaaaattaa caaac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
