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Gene information
General information
TC005895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5900368..5915555
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005895
    iBeetle screen iB_00956

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature mostly degenerated - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Adult morphology
    ovipositor size constricted - Number of animals showing the phenotype: 1


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005895 RA
      MAMQREVGV GDFVLLDKI DLGNFMKNL ELRFKHGKI 
      YTYIGEVCV SVNPYKTMN IYDQKFIDQ YKGRELFEN 
      PPHIFAIAD ASHKVMQQQ GKDTCIVIS GESGAGKTE 
      ASKIIMKYI AAVTNKDGQ KEIERVKNV LLQSNSILE 
      AFGNAKTNR NDNSSRFGK YMDIHFDFK GDPIGGHIN 
      KYLLEKSRV IYQQAGERN FHSFYQVLA GAGDDLLRK 
      LHLSRNSDD YFYVKQGKA AKVDTINDR NDFRDVNSS 
      LNTLQFSKE AQETLWRVV AAILHLGNL EFVTDGDNK 
      LQIKNKKSL EIISELLQV QKNELNSAL CERVIAARG 
      DIMRKEHTE AEALYGRDA LAKAIYERL FGWIVEKIN 
      SAIAIDEKN YSKNYKSSL IGVLDIYGF EIFDNNSFE 
      QFCINYCNE KLQQLFIEL VLKQEQEEY NREGIEWQN 
      IDFFNNQII CELVEAPHK GILAIMDDA SKMTAEKVT 
      DELLLETMD KQLKNHKHY TSRQLKPAD KNMRHKIDF 
      RITHYAGDV TYCVTGFLD KNKDTLFQD FKRLMFNSN 
      DSNLKEMFP EGSQHISQT TKRPPTAGS LFKTSMQAL 
      VQNLYSKEP HYVRCIKPN ELKSPSAFD EERVKHQVS 
      YLGLVENVR VRRAGFAYR QRYDLFLKR YKMISQFTW 
      PNFRSGTDK DGVCVIMDE RKFSDDVKY GKTKIFIRS 
      PKTLFALEA ARNDLIPGI VTLIQKTWR GYVARQQYK 
      KMRALMTMI QAYRLKKMR VYVNALQAK FANAKRMKD 
      FGKSVMWPA PPVSLQKTT RTLKQIYNR WRAFMILNK 
      IPRAEWPEM KIKVAAASA LLNKRPTYG LNRRWEGNY 
      LATHSENNN YTTFNDAVN NLKNGQHFG KVLFSSFVT 
      KFNKFNKCA ERVILVTDL AIFKLDSLK FRNMKEGTA 
      VKDLTGISV SPGQDQLIV LHCLGGNDL VVSLHSMKQ 
      EDRIGELVG IVSNTYFKF KGTDLPVTV SKTITCSLG 
      GKKRVITIN VTSEVQTPT FKKDGNNIA YLLPTSFAV 
      VENGRDFIK H
    CDS sequence
    • >TC005895 RA
      atggcaatg caacgagaa gtgggcgtg ggcgacttc 
      gtccttttg gacaagatc gatttgggc aactttatg 
      aaaaatcta gagctcaga ttcaaacat ggcaagatt 
      tacacctac attggcgag gtatgcgtt tcggtaaat 
      ccctacaaa acgatgaat atctacgac caaaaattc 
      atcgatcaa tataaaggt cgcgagctg ttcgaaaac 
      cctcctcac atcttcgcc atcgccgac gcctctcac 
      aaagtcatg cagcagcaa ggcaaggac acttgtatc 
      gtaatcagc ggcgaatca ggagccgga aaaacagaa 
      gcctcgaaa ataataatg aaatatatc gcggccgtg 
      acgaacaaa gacggccag aaggagatc gaacgcgtg 
      aagaacgtc cttttgcaa tcaaactcg attctagaa 
      gcgttcgga aacgcaaaa accaaccgc aacgacaat 
      tcctcgcgt tttgggaag tacatggac atccatttc 
      gatttcaaa ggcgacccg atcggcgga cacatcaac 
      aagtatctg ctcgaaaaa tcccgagtg atttaccag 
      caagccggc gagaggaac ttccacagt ttctaccag 
      gttttggcg ggcgctggc gacgatctt ttgaggaaa 
      ctgcacttg agtcgcaac agtgatgac tatttttac 
      gtcaaacag gggaaagcc gccaaagtc gacacgatc 
      aatgaccga aatgacttc cgggatgtg aattcgtca 
      ctgaacacg ttgcagttc tcgaaggaa gcgcaagaa 
      actctatgg agggttgtg gcagcgatt ttgcatctt 
      ggaaatttg gaatttgtt acagacggt gacaataaa 
      ttgcaaatt aagaacaag aaatcgctc gaaataatt 
      tcggagctg cttcaagtg cagaaaaac gagctaaat 
      tcggcgctt tgtgagcgc gtgattgcc gccagaggc 
      gacattatg aggaaggaa cacaccgaa gctgaggcg 
      ctttacgga cgcgatgcc ctagctaag gccatttac 
      gagcgactt ttcggttgg attgtcgag aaaatcaac 
      tcggcgatc gccatcgac gagaaaaac tattcgaaa 
      aactacaaa agctcgctg attggagtg ttggacatc 
      tacgggttt gagattttc gacaacaac agtttcgag 
      cagttctgt atcaactac tgtaacgag aagttgcag 
      cagttgttt attgaactt gtcctgaag caggagcaa 
      gaggagtat aatcgcgaa ggcatcgag tggcaaaac 
      atcgatttc ttcaacaat cagatcatt tgtgagctt 
      gttgaagcg ccacataag gggattttg gcgattatg 
      gatgatgcg tcgaaaatg actgccgag aaagttact 
      gatgagctt ttactagaa acgatggat aagcagttg 
      aaaaatcat aagcattat acgtcgaga caactcaaa 
      ccggctgat aaaaatatg aggcataag atcgatttt 
      cggataaca cattatgcg ggtgatgtc acctattgt 
      gtaacggga tttttggat aagaataag gatacgctc 
      ttccaggat tttaaacgg ttaatgttt aattccaac 
      gattcgaat ttgaaggag atgttccca gaaggctcg 
      caacacatt tcacagacc accaaacgc cccccaacg 
      gccggctcc ctgttcaaa acctccatg caagccctc 
      gtccagaac ctctacagc aaagagccg cactacgtc 
      cgctgcatc aaacccaac gaactgaaa tccccgtcg 
      gccttcgac gaagaacgc gtcaagcac caggtcagc 
      tacttgggt ctggtggaa aacgtgcgc gtccgtcgc 
      gctggtttc gcctaccgt cagcgttac gacctcttc 
      ctcaaacgc tacaaaatg atctcacag ttcacgtgg 
      cccaacttc cgttccggc accgataaa gacggcgtt 
      tgcgtcata atggacgaa cgcaaattc agcgatgac 
      gtaaaatac ggcaaaacg aaaatattc attcgctcg 
      ccgaaaacg ctattcgca ctggaagcg gcgcgaaat 
      gacctcatc cccggcatt gtgactttg attcagaaa 
      acgtggcgc ggctacgtc gccaggcaa cagtacaag 
      aagatgagg gccttaatg acgatgatt caagcgtat 
      aggttgaag aaaatgcgc gtttatgtg aacgcactc 
      caggcgaag ttcgcaaac gcgaaacga atgaaagac 
      ttcgggaag agcgtcatg tggccagcg ccgcccgtg 
      tcgctgcag aagacaaca cgcactttg aagcagatt 
      tacaatcgc tggagggcc ttcatgata cttaataaa 
      atcccgagg gccgagtgg cccgaaatg aaaatcaag 
      gtggccgcg gccagtgcc ttactcaac aaacggccg 
      acttacgga ctcaacaga aggtgggag ggtaattat 
      cttgccacg cattctgaa aataacaat tacacgacg 
      ttcaacgat gcggttaat aacttgaaa aatggccaa 
      cactttgga aaagtgctg ttttcgtcg ttcgttacg 
      aaattcaac aagtttaat aagtgtgca gagcgagtg 
      attctcgtt actgatctg gcgattttc aagcttgat 
      tcgttgaag ttcaggaat atgaaagaa gggacggct 
      gttaaggac ctgacaggg attagtgtc agtccggga 
      caggatcaa ttgatcgtt ttacattgt ttgggtggg 
      aatgattta gttgtgtcg ttgcattcg atgaagcaa 
      gaggatcgg attggggaa ttggttggg attgtctcc 
      aacacatat ttcaagttc aagggaacg gatcttccg 
      gtcaccgtc agcaaaacc attacctgt tcactgggt 
      gggaaaaag cgtgtaatt acaatcaac gtgacttcc 
      gaggtacaa acgccaacg ttcaagaag gacggaaat 
      aacattgcg tatttgtta ccgacaagt tttgccgtt 
      gtggaaaac ggtcgtgat tttatcaag cattaa
    mRNA sequence
    • >TC005895 RA
      atcggcagt caaacaacg gccggtgtg agaatgaga 
      acatttcaa acaaacgaa cagttttat taaaattga 
      aatggcaat gcaacgaga agtgggcgt gggcgactt 
      cgtcctttt ggacaagat cgatttggg caactttat 
      gaaaaatct agagctcag attcaaaca tggcaagat 
      ttacaccta cattggcga ggtatgcgt ttcggtaaa 
      tccctacaa aacgatgaa tatctacga ccaaaaatt 
      catcgatca atataaagg tcgcgagct gttcgaaaa 
      ccctcctca catcttcgc catcgccga cgcctctca 
      caaagtcat gcagcagca aggcaagga cacttgtat 
      cgtaatcag cggcgaatc aggagccgg aaaaacaga 
      agcctcgaa aataataat gaaatatat cgcggccgt 
      gacgaacaa agacggcca gaaggagat cgaacgcgt 
      gaagaacgt ccttttgca atcaaactc gattctaga 
      agcgttcgg aaacgcaaa aaccaaccg caacgacaa 
      ttcctcgcg ttttgggaa gtacatgga catccattt 
      cgatttcaa aggcgaccc gatcggcgg acacatcaa 
      caagtatct gctcgaaaa atcccgagt gatttacca 
      gcaagccgg cgagaggaa cttccacag tttctacca 
      ggttttggc gggcgctgg cgacgatct tttgaggaa 
      actgcactt gagtcgcaa cagtgatga ctattttta 
      cgtcaaaca ggggaaagc cgccaaagt cgacacgat 
      caatgaccg aaatgactt ccgggatgt gaattcgtc 
      actgaacac gttgcagtt ctcgaagga agcgcaaga 
      aactctatg gagggttgt ggcagcgat tttgcatct 
      tggaaattt ggaatttgt tacagacgg tgacaataa 
      attgcaaat taagaacaa gaaatcgct cgaaataat 
      ttcggagct gcttcaagt gcagaaaaa cgagctaaa 
      ttcggcgct ttgtgagcg cgtgattgc cgccagagg 
      cgacattat gaggaagga acacaccga agctgaggc 
      gctttacgg acgcgatgc cctagctaa ggccattta 
      cgagcgact tttcggttg gattgtcga gaaaatcaa 
      ctcggcgat cgccatcga cgagaaaaa ctattcgaa 
      aaactacaa aagctcgct gattggagt gttggacat 
      ctacgggtt tgagatttt cgacaacaa cagtttcga 
      gcagttctg tatcaacta ctgtaacga gaagttgca 
      gcagttgtt tattgaact tgtcctgaa gcaggagca 
      agaggagta taatcgcga aggcatcga gtggcaaaa 
      catcgattt cttcaacaa tcagatcat ttgtgagct 
      tgttgaagc gccacataa ggggatttt ggcgattat 
      ggatgatgc gtcgaaaat gactgccga gaaagttac 
      tgatgagct tttactaga aacgatgga taagcagtt 
      gaaaaatca taagcatta tacgtcgag acaactcaa 
      accggctga taaaaatat gaggcataa gatcgattt 
      tcggataac acattatgc gggtgatgt cacctattg 
      tgtaacggg atttttgga taagaataa ggatacgct 
      cttccagga ttttaaacg gttaatgtt taattccaa 
      cgattcgaa tttgaagga gatgttccc agaaggctc 
      gcaacacat ttcacagac caccaaacg ccccccaac 
      ggccggctc cctgttcaa aacctccat gcaagccct 
      cgtccagaa cctctacag caaagagcc gcactacgt 
      ccgctgcat caaacccaa cgaactgaa atccccgtc 
      ggccttcga cgaagaacg cgtcaagca ccaggtcag 
      ctacttggg tctggtgga aaacgtgcg cgtccgtcg 
      cgctggttt cgcctaccg tcagcgtta cgacctctt 
      cctcaaacg ctacaaaat gatctcaca gttcacgtg 
      gcccaactt ccgttccgg caccgataa agacggcgt 
      ttgcgtcat aatggacga acgcaaatt cagcgatga 
      cgtaaaata cggcaaaac gaaaatatt cattcgctc 
      gccgaaaac gctattcgc actggaagc ggcgcgaaa 
      tgacctcat ccccggcat tgtgacttt gattcagaa 
      aacgtggcg cggctacgt cgccaggca acagtacaa 
      gaagatgag ggccttaat gacgatgat tcaagcgta 
      taggttgaa gaaaatgcg cgtttatgt gaacgcact 
      ccaggcgaa gttcgcaaa cgcgaaacg aatgaaaga 
      cttcgggaa gagcgtcat gtggccagc gccgcccgt 
      gtcgctgca gaagacaac acgcacttt gaagcagat 
      ttacaatcg ctggagggc cttcatgat acttaataa 
      aatcccgag ggccgagtg gcccgaaat gaaaatcaa 
      ggtggccgc ggccagtgc cttactcaa caaacggcc 
      gacttacgg actcaacag aaggtggga gggtaatta 
      tcttgccac gcattctga aaataacaa ttacacgac 
      gttcaacga tgcggttaa taacttgaa aaatggcca 
      acactttgg aaaagtgct gttttcgtc gttcgttac 
      gaaattcaa caagtttaa taagtgtgc agagcgagt 
      gattctcgt tactgatct ggcgatttt caagcttga 
      ttcgttgaa gttcaggaa tatgaaaga agggacggc 
      tgttaagga cctgacagg gattagtgt cagtccggg 
      acaggatca attgatcgt tttacattg tttgggtgg 
      gaatgattt agttgtgtc gttgcattc gatgaagca 
      agaggatcg gattgggga attggttgg gattgtctc 
      caacacata tttcaagtt caagggaac ggatcttcc 
      ggtcaccgt cagcaaaac cattacctg ttcactggg 
      tgggaaaaa gcgtgtaat tacaatcaa cgtgacttc 
      cgaggtaca aacgccaac gttcaagaa ggacggaaa 
      taacattgc gtatttgtt accgacaag ttttgccgt 
      tgtggaaaa cggtcgtga ttttatcaa gcattaatt 
      cgtgccttt tttacaaga tggttcaga tttatatgt 
      atacaaaca acgaatttg ttgcttttt atgtactta 
      aatgtgcga tctttgaga tcatatgtg ttgtcatca 
      gtttgaaat tttatttta aatgtcgtg ttacgtagt 
      tttatgaat ttatatatc gttgttaat cattccacg 
      atggcattt tgttgattc attattttc tactaaaag 
      tattcgcat ctggtatta tgtaaaagt gattgttgt 
      atttatttg tatatacaa gtgccattt tttgtttat 
      atttttgta tcgattttg gatacatag tcaagctaa 
      agtacgtgc cttaataaa aaaaattaa caaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY