Status
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Gene information
General information
TC005895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5900368..5915555
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005895
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00956

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature mostly degenerated - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    Adult morphology
    ovipositor size constricted - Number of animals showing the phenotype: 1


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005895 RA
      MAMQREVGV GDFVLLDKI DLGNFMKNL ELRFKHGKI 
YTYIGEVCV SVNPYKTMN IYDQKFIDQ YKGRELFEN 
PPHIFAIAD ASHKVMQQQ GKDTCIVIS GESGAGKTE 
ASKIIMKYI AAVTNKDGQ KEIERVKNV LLQSNSILE 
AFGNAKTNR NDNSSRFGK YMDIHFDFK GDPIGGHIN 
KYLLEKSRV IYQQAGERN FHSFYQVLA GAGDDLLRK 
LHLSRNSDD YFYVKQGKA AKVDTINDR NDFRDVNSS 
LNTLQFSKE AQETLWRVV AAILHLGNL EFVTDGDNK 
LQIKNKKSL EIISELLQV QKNELNSAL CERVIAARG 
DIMRKEHTE AEALYGRDA LAKAIYERL FGWIVEKIN 
SAIAIDEKN YSKNYKSSL IGVLDIYGF EIFDNNSFE 
QFCINYCNE KLQQLFIEL VLKQEQEEY NREGIEWQN 
IDFFNNQII CELVEAPHK GILAIMDDA SKMTAEKVT 
DELLLETMD KQLKNHKHY TSRQLKPAD KNMRHKIDF 
RITHYAGDV TYCVTGFLD KNKDTLFQD FKRLMFNSN 
DSNLKEMFP EGSQHISQT TKRPPTAGS LFKTSMQAL 
VQNLYSKEP HYVRCIKPN ELKSPSAFD EERVKHQVS 
YLGLVENVR VRRAGFAYR QRYDLFLKR YKMISQFTW 
PNFRSGTDK DGVCVIMDE RKFSDDVKY GKTKIFIRS 
PKTLFALEA ARNDLIPGI VTLIQKTWR GYVARQQYK 
KMRALMTMI QAYRLKKMR VYVNALQAK FANAKRMKD 
FGKSVMWPA PPVSLQKTT RTLKQIYNR WRAFMILNK 
IPRAEWPEM KIKVAAASA LLNKRPTYG LNRRWEGNY 
LATHSENNN YTTFNDAVN NLKNGQHFG KVLFSSFVT 
KFNKFNKCA ERVILVTDL AIFKLDSLK FRNMKEGTA 
VKDLTGISV SPGQDQLIV LHCLGGNDL VVSLHSMKQ 
EDRIGELVG IVSNTYFKF KGTDLPVTV SKTITCSLG 
GKKRVITIN VTSEVQTPT FKKDGNNIA YLLPTSFAV 
VENGRDFIK H
    CDS sequence
    • >TC005895 RA
      atggcaatg caacgagaa gtgggcgtg ggcgacttc 
gtccttttg gacaagatc gatttgggc aactttatg 
aaaaatcta gagctcaga ttcaaacat ggcaagatt 
tacacctac attggcgag gtatgcgtt tcggtaaat 
ccctacaaa acgatgaat atctacgac caaaaattc 
atcgatcaa tataaaggt cgcgagctg ttcgaaaac 
cctcctcac atcttcgcc atcgccgac gcctctcac 
aaagtcatg cagcagcaa ggcaaggac acttgtatc 
gtaatcagc ggcgaatca ggagccgga aaaacagaa 
gcctcgaaa ataataatg aaatatatc gcggccgtg 
acgaacaaa gacggccag aaggagatc gaacgcgtg 
aagaacgtc cttttgcaa tcaaactcg attctagaa 
gcgttcgga aacgcaaaa accaaccgc aacgacaat 
tcctcgcgt tttgggaag tacatggac atccatttc 
gatttcaaa ggcgacccg atcggcgga cacatcaac 
aagtatctg ctcgaaaaa tcccgagtg atttaccag 
caagccggc gagaggaac ttccacagt ttctaccag 
gttttggcg ggcgctggc gacgatctt ttgaggaaa 
ctgcacttg agtcgcaac agtgatgac tatttttac 
gtcaaacag gggaaagcc gccaaagtc gacacgatc 
aatgaccga aatgacttc cgggatgtg aattcgtca 
ctgaacacg ttgcagttc tcgaaggaa gcgcaagaa 
actctatgg agggttgtg gcagcgatt ttgcatctt 
ggaaatttg gaatttgtt acagacggt gacaataaa 
ttgcaaatt aagaacaag aaatcgctc gaaataatt 
tcggagctg cttcaagtg cagaaaaac gagctaaat 
tcggcgctt tgtgagcgc gtgattgcc gccagaggc 
gacattatg aggaaggaa cacaccgaa gctgaggcg 
ctttacgga cgcgatgcc ctagctaag gccatttac 
gagcgactt ttcggttgg attgtcgag aaaatcaac 
tcggcgatc gccatcgac gagaaaaac tattcgaaa 
aactacaaa agctcgctg attggagtg ttggacatc 
tacgggttt gagattttc gacaacaac agtttcgag 
cagttctgt atcaactac tgtaacgag aagttgcag 
cagttgttt attgaactt gtcctgaag caggagcaa 
gaggagtat aatcgcgaa ggcatcgag tggcaaaac 
atcgatttc ttcaacaat cagatcatt tgtgagctt 
gttgaagcg ccacataag gggattttg gcgattatg 
gatgatgcg tcgaaaatg actgccgag aaagttact 
gatgagctt ttactagaa acgatggat aagcagttg 
aaaaatcat aagcattat acgtcgaga caactcaaa 
ccggctgat aaaaatatg aggcataag atcgatttt 
cggataaca cattatgcg ggtgatgtc acctattgt 
gtaacggga tttttggat aagaataag gatacgctc 
ttccaggat tttaaacgg ttaatgttt aattccaac 
gattcgaat ttgaaggag atgttccca gaaggctcg 
caacacatt tcacagacc accaaacgc cccccaacg 
gccggctcc ctgttcaaa acctccatg caagccctc 
gtccagaac ctctacagc aaagagccg cactacgtc 
cgctgcatc aaacccaac gaactgaaa tccccgtcg 
gccttcgac gaagaacgc gtcaagcac caggtcagc 
tacttgggt ctggtggaa aacgtgcgc gtccgtcgc 
gctggtttc gcctaccgt cagcgttac gacctcttc 
ctcaaacgc tacaaaatg atctcacag ttcacgtgg 
cccaacttc cgttccggc accgataaa gacggcgtt 
tgcgtcata atggacgaa cgcaaattc agcgatgac 
gtaaaatac ggcaaaacg aaaatattc attcgctcg 
ccgaaaacg ctattcgca ctggaagcg gcgcgaaat 
gacctcatc cccggcatt gtgactttg attcagaaa 
acgtggcgc ggctacgtc gccaggcaa cagtacaag 
aagatgagg gccttaatg acgatgatt caagcgtat 
aggttgaag aaaatgcgc gtttatgtg aacgcactc 
caggcgaag ttcgcaaac gcgaaacga atgaaagac 
ttcgggaag agcgtcatg tggccagcg ccgcccgtg 
tcgctgcag aagacaaca cgcactttg aagcagatt 
tacaatcgc tggagggcc ttcatgata cttaataaa 
atcccgagg gccgagtgg cccgaaatg aaaatcaag 
gtggccgcg gccagtgcc ttactcaac aaacggccg 
acttacgga ctcaacaga aggtgggag ggtaattat 
cttgccacg cattctgaa aataacaat tacacgacg 
ttcaacgat gcggttaat aacttgaaa aatggccaa 
cactttgga aaagtgctg ttttcgtcg ttcgttacg 
aaattcaac aagtttaat aagtgtgca gagcgagtg 
attctcgtt actgatctg gcgattttc aagcttgat 
tcgttgaag ttcaggaat atgaaagaa gggacggct 
gttaaggac ctgacaggg attagtgtc agtccggga 
caggatcaa ttgatcgtt ttacattgt ttgggtggg 
aatgattta gttgtgtcg ttgcattcg atgaagcaa 
gaggatcgg attggggaa ttggttggg attgtctcc 
aacacatat ttcaagttc aagggaacg gatcttccg 
gtcaccgtc agcaaaacc attacctgt tcactgggt 
gggaaaaag cgtgtaatt acaatcaac gtgacttcc 
gaggtacaa acgccaacg ttcaagaag gacggaaat 
aacattgcg tatttgtta ccgacaagt tttgccgtt 
gtggaaaac ggtcgtgat tttatcaag cattaa
    mRNA sequence
    • >TC005895 RA
      atcggcagt caaacaacg gccggtgtg agaatgaga 
acatttcaa acaaacgaa cagttttat taaaattga 
aatggcaat gcaacgaga agtgggcgt gggcgactt 
cgtcctttt ggacaagat cgatttggg caactttat 
gaaaaatct agagctcag attcaaaca tggcaagat 
ttacaccta cattggcga ggtatgcgt ttcggtaaa 
tccctacaa aacgatgaa tatctacga ccaaaaatt 
catcgatca atataaagg tcgcgagct gttcgaaaa 
ccctcctca catcttcgc catcgccga cgcctctca 
caaagtcat gcagcagca aggcaagga cacttgtat 
cgtaatcag cggcgaatc aggagccgg aaaaacaga 
agcctcgaa aataataat gaaatatat cgcggccgt 
gacgaacaa agacggcca gaaggagat cgaacgcgt 
gaagaacgt ccttttgca atcaaactc gattctaga 
agcgttcgg aaacgcaaa aaccaaccg caacgacaa 
ttcctcgcg ttttgggaa gtacatgga catccattt 
cgatttcaa aggcgaccc gatcggcgg acacatcaa 
caagtatct gctcgaaaa atcccgagt gatttacca 
gcaagccgg cgagaggaa cttccacag tttctacca 
ggttttggc gggcgctgg cgacgatct tttgaggaa 
actgcactt gagtcgcaa cagtgatga ctattttta 
cgtcaaaca ggggaaagc cgccaaagt cgacacgat 
caatgaccg aaatgactt ccgggatgt gaattcgtc 
actgaacac gttgcagtt ctcgaagga agcgcaaga 
aactctatg gagggttgt ggcagcgat tttgcatct 
tggaaattt ggaatttgt tacagacgg tgacaataa 
attgcaaat taagaacaa gaaatcgct cgaaataat 
ttcggagct gcttcaagt gcagaaaaa cgagctaaa 
ttcggcgct ttgtgagcg cgtgattgc cgccagagg 
cgacattat gaggaagga acacaccga agctgaggc 
gctttacgg acgcgatgc cctagctaa ggccattta 
cgagcgact tttcggttg gattgtcga gaaaatcaa 
ctcggcgat cgccatcga cgagaaaaa ctattcgaa 
aaactacaa aagctcgct gattggagt gttggacat 
ctacgggtt tgagatttt cgacaacaa cagtttcga 
gcagttctg tatcaacta ctgtaacga gaagttgca 
gcagttgtt tattgaact tgtcctgaa gcaggagca 
agaggagta taatcgcga aggcatcga gtggcaaaa 
catcgattt cttcaacaa tcagatcat ttgtgagct 
tgttgaagc gccacataa ggggatttt ggcgattat 
ggatgatgc gtcgaaaat gactgccga gaaagttac 
tgatgagct tttactaga aacgatgga taagcagtt 
gaaaaatca taagcatta tacgtcgag acaactcaa 
accggctga taaaaatat gaggcataa gatcgattt 
tcggataac acattatgc gggtgatgt cacctattg 
tgtaacggg atttttgga taagaataa ggatacgct 
cttccagga ttttaaacg gttaatgtt taattccaa 
cgattcgaa tttgaagga gatgttccc agaaggctc 
gcaacacat ttcacagac caccaaacg ccccccaac 
ggccggctc cctgttcaa aacctccat gcaagccct 
cgtccagaa cctctacag caaagagcc gcactacgt 
ccgctgcat caaacccaa cgaactgaa atccccgtc 
ggccttcga cgaagaacg cgtcaagca ccaggtcag 
ctacttggg tctggtgga aaacgtgcg cgtccgtcg 
cgctggttt cgcctaccg tcagcgtta cgacctctt 
cctcaaacg ctacaaaat gatctcaca gttcacgtg 
gcccaactt ccgttccgg caccgataa agacggcgt 
ttgcgtcat aatggacga acgcaaatt cagcgatga 
cgtaaaata cggcaaaac gaaaatatt cattcgctc 
gccgaaaac gctattcgc actggaagc ggcgcgaaa 
tgacctcat ccccggcat tgtgacttt gattcagaa 
aacgtggcg cggctacgt cgccaggca acagtacaa 
gaagatgag ggccttaat gacgatgat tcaagcgta 
taggttgaa gaaaatgcg cgtttatgt gaacgcact 
ccaggcgaa gttcgcaaa cgcgaaacg aatgaaaga 
cttcgggaa gagcgtcat gtggccagc gccgcccgt 
gtcgctgca gaagacaac acgcacttt gaagcagat 
ttacaatcg ctggagggc cttcatgat acttaataa 
aatcccgag ggccgagtg gcccgaaat gaaaatcaa 
ggtggccgc ggccagtgc cttactcaa caaacggcc 
gacttacgg actcaacag aaggtggga gggtaatta 
tcttgccac gcattctga aaataacaa ttacacgac 
gttcaacga tgcggttaa taacttgaa aaatggcca 
acactttgg aaaagtgct gttttcgtc gttcgttac 
gaaattcaa caagtttaa taagtgtgc agagcgagt 
gattctcgt tactgatct ggcgatttt caagcttga 
ttcgttgaa gttcaggaa tatgaaaga agggacggc 
tgttaagga cctgacagg gattagtgt cagtccggg 
acaggatca attgatcgt tttacattg tttgggtgg 
gaatgattt agttgtgtc gttgcattc gatgaagca 
agaggatcg gattgggga attggttgg gattgtctc 
caacacata tttcaagtt caagggaac ggatcttcc 
ggtcaccgt cagcaaaac cattacctg ttcactggg 
tgggaaaaa gcgtgtaat tacaatcaa cgtgacttc 
cgaggtaca aacgccaac gttcaagaa ggacggaaa 
taacattgc gtatttgtt accgacaag ttttgccgt 
tgtggaaaa cggtcgtga ttttatcaa gcattaatt 
cgtgccttt tttacaaga tggttcaga tttatatgt 
atacaaaca acgaatttg ttgcttttt atgtactta 
aatgtgcga tctttgaga tcatatgtg ttgtcatca 
gtttgaaat tttatttta aatgtcgtg ttacgtagt 
tttatgaat ttatatatc gttgttaat cattccacg 
atggcattt tgttgattc attattttc tactaaaag 
tattcgcat ctggtatta tgtaaaagt gattgttgt 
atttatttg tatatacaa gtgccattt tttgtttat 
atttttgta tcgattttg gatacatag tcaagctaa 
agtacgtgc cttaataaa aaaaattaa caaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY