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Gene information
General information
TC005894 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid53: 24322..37257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005894
    iBeetle screen iB_00955
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages partially orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005894 RA
      MEQFSKGKP VTTSVPTIS IKFHANAEQ LEELYASDD 
      EHNANDAAT AQTGAAGVS EPESSPLKL LPRLVKRST 
      SIDITPNPN IDASPPSDP WRFFSDIKG KITKSVEEK 
      ITEIKARNQ DEGSPLKTK VKDSKENSS LSDSEDLSE 
      SSISKTCGI FSTTEGAEM SSDDDDTPS IEKDRNVGL 
      RQRFRLLKS KSSKEGTIS KKSLASIYS INSDKIEQA 
      LPEENDIES AVDALEEIG ANFKILKEI EQDFDNIKI 
      DDENVVNIK EVSGEEIRE IQSKPQTVY APVGFVDLR 
      ARNETIKKG VSRGFFHIF AIVLAYVLI HSYSSYLAG 
      FLIGVFVTC VFYYVKFLL DTPSHSPAI AIQTCKDIL 
      QVPAVKEFQ PLTKYEGWV NEFPDNYNP HTYHISQTQ 
      SVFLRLQGN LLRLSHTKS KVAKRALWN EPKIKPIFT 
      HHRVYNLTG AQVSLLPVG LAKKRHWSK KYPICVTLN 
      KDQMDFIES PIEKKRSQG NKKKFSILA QRFSKLTEE 
      NEDFECDSE NSRASSPSP PSPDDSDDK LLNEALDDA 
      LKDEENEVL LDEEFESIP DDWSGLTPP DESPTQTKL 
      FLFGRTDRE KEDWFRRLK AATHKGANL PSSDTCEIL 
      TESDYIKYM MIFNKIKVE SDVQWINSL VGRVFFDCL 
      RDDNFTQGI KDKIQSKLS SIKLPYFIE GLMVTELNL 
      GKTPPLIHK VTKPSIDER GLWIDLDVT YEGLVVLIL 
      QTKLNLMKL KQPHSNETV KERSAMFNS DLEDSPEST 
      SDEETPIFP NPPALDSGQ SGSNTSKKF IKMVDRIAE 
      SKFFQAATE YRYIKKAME GVSNTDLRL KVEIKELVG 
      TLVLNIPPP PSDRVWIGF RPTPELALG AQPIVGERN 
      ITFLRITSW IEKKLRLEF QKLLVIPNM EDFIVPVMS 
      PKLPE
    CDS sequence
    • >TC005894 RA
      atggaacaa ttcagtaaa ggtaaacct gtcaccact 
      tcagttcct acaatttcc attaaattc cacgcaaat 
      gctgaacaa ttggaagag ttgtacgcg agcgatgat 
      gaacacaac gccaacgac gccgctaca gcacagacg 
      ggggccgca ggagttagc gagcccgag agtagcccc 
      ttgaagttg cttcctcgt ctcgtcaaa cgttccaca 
      agtatagac atcactccg aacccaaac atcgatgct 
      tcgccccct tccgacccg tggcgattt ttttccgac 
      attaaaggt aaaataacc aaaagtgtg gaggaaaaa 
      atcacagag ataaaagcg cgtaaccag gacgaaggt 
      tcgccactt aaaaccaaa gttaaagac agtaaagaa 
      aattcgagt ttgtccgat tcagaggac ttatccgag 
      agtagtatt tcgaaaact tgtggcatt ttttcgaca 
      actgaaggt gcggaaatg tccagtgac gatgatgac 
      actccgtca attgaaaag gaccgaaat gtcggttta 
      aggcaaagg tttaggttg ctcaagtcg aagtcaagt 
      aaagaaggg acgattagt aagaagagt ttagcgagc 
      atttatagc ataaatagt gataaaatc gagcaagct 
      ttgccggaa gaaaacgat attgagagt gctgtggac 
      gcgttggag gaaattggc gccaatttt aaaattctc 
      aaagaaatt gagcaagat tttgataac attaaaatc 
      gacgatgag aatgttgtc aatatcaag gaagtgagt 
      ggtgaggaa atcagggaa atccagtcg aaaccacag 
      accgtgtac gcacctgtt ggtttcgtc gatttaagg 
      gcgagaaat gaaacgata aaaaagggt gtttcccga 
      gggtttttt cacattttt gccattgtc ctggcttat 
      gttttaatc cacagttac tcgagttat ttggctggg 
      tttttgatc ggtgttttt gtgacttgt gtcttttat 
      tatgtgaaa tttttgctc gatactccg tctcatagc 
      ccggcaatt gccatccag acgtgcaaa gatatttta 
      caagtaccg gcagtgaag gaatttcaa ccgttgact 
      aaatacgaa ggttgggtt aatgaattt cccgataat 
      tacaatcct cacacttac cacatcagt caaacacaa 
      tcggtgttt ttgcgtttg caaggcaat ttacttcgt 
      ctgagtcac acaaaatcg aaagttgcc aaaagggcg 
      ctttggaac gaaccgaaa atcaaaccg attttcaca 
      catcatcgg gtttataat ctaactggg gcacaagtg 
      agtttgttg ccggttggt ttggcgaag aagcgtcat 
      tggagtaag aaatatccg atttgtgtg actttgaac 
      aaggatcaa atggatttt attgaatca ccaatcgag 
      aaaaaacgc agtcaaggg aataagaaa aagttttcg 
      attctggca caaaggttt agtaagttg acggaagaa 
      aacgaagat tttgagtgt gattcggag aattcgagg 
      gcgagtagt ccctcacca ccatcgcca gacgattca 
      gatgataaa ttgttgaac gaagccttg gatgatgct 
      ttgaaagac gaggaaaac gaggtttta ctcgacgaa 
      gagtttgag tcgattccg gacgattgg agcggtttg 
      acaccacca gatgaaagt ccgacccaa acgaaacta 
      ttccttttc ggacgaact gaccgagag aaagaagac 
      tggttcagg agacttaag gcggccaca cataaaggt 
      gcaaactta ccgtcaagt gacacctgc gagattttg 
      actgaaagt gactacata aagtatatg atgattttt 
      aataaaatt aaggtcgaa agtgacgtc cagtggatc 
      aattctttg gtcggtcga gtctttttc gattgttta 
      cgtgacgat aacttcact caaggaatc aaggataaa 
      atccagagt aaattatcg tcgattaag cttccatac 
      tttattgaa ggtttgatg gtgactgaa ctaaatttg 
      ggtaaaacc ccgcctttg attcacaaa gtaacaaaa 
      ccgagcata gacgaacgc gggttgtgg atcgatctc 
      gacgtaaca tacgaaggt ttagtcgtt ttaattttg 
      caaactaaa ctcaactta atgaaactg aagcagcca 
      cactctaac gaaacggta aaagaacgt tcggcgatg 
      ttcaattcc gatcttgag gattcccct gaaagcacc 
      tctgatgag gaaactccg attttcccc aatcctccg 
      gcactggat tccggtcaa agcgggagc aatacgagt 
      aaaaaattc atcaaaatg gtagaccgc atagcggaa 
      tccaagttt ttccaagcc gccaccgaa taccgttat 
      ataaaaaaa gcgatggag ggggtttcc aacaccgat 
      ttgcgactt aaagtcgag attaaggaa ctggtgggc 
      actctagtt ttgaacatt ccgccgcct ccgagtgac 
      cgtgtttgg attggcttt cgaccaacc cccgagctg 
      gctctgggg gcgcaaccg atcgtaggc gaacgcaat 
      atcacgttt ttgcggatt accagttgg atcgagaag 
      aagttgcgt ttggagttc cagaaattg ttagtgatt 
      cccaacatg gaagacttc attgtacct gtgatgagt 
      ccaaaacta ccggaataa
    mRNA sequence
    • >TC005894 RA
      cctagttta ggaaataat gttggcgac agtgcgtca 
      tgacagggc cgatttcca aatgacagg agtaaacga 
      acgtaatta aattttaca gaattattc gtaatttaa 
      aagtgaatt gcttgttgt caagtggag aagatagta 
      aaaaaatcg attctggtg ttatttgca ccgagtgtt 
      gcccaacgc acggagttg taatgttgc aaaatggaa 
      caattcagt aaaggtaaa cctgtcacc acttcagtt 
      cctacaatt tccattaaa ttccacgca aatgctgaa 
      caattggaa gagttgtac gcgagcgat gatgaacac 
      aacgccaac gacgccgct acagcacag acgggggcc 
      gcaggagtt agcgagccc gagagtagc cccttgaag 
      ttgcttcct cgtctcgtc aaacgttcc acaagtata 
      gacatcact ccgaaccca aacatcgat gcttcgccc 
      ccttccgac ccgtggcga tttttttcc gacattaaa 
      ggtaaaata accaaaagt gtggaggaa aaaatcaca 
      gagataaaa gcgcgtaac caggacgaa ggttcgcca 
      cttaaaacc aaagttaaa gacagtaaa gaaaattcg 
      agtttgtcc gattcagag gacttatcc gagagtagt 
      atttcgaaa acttgtggc attttttcg acaactgaa 
      ggtgcggaa atgtccagt gacgatgat gacactccg 
      tcaattgaa aaggaccga aatgtcggt ttaaggcaa 
      aggtttagg ttgctcaag tcgaagtca agtaaagaa 
      gggacgatt agtaagaag agtttagcg agcatttat 
      agcataaat agtgataaa atcgagcaa gctttgccg 
      gaagaaaac gatattgag agtgctgtg gacgcgttg 
      gaggaaatt ggcgccaat tttaaaatt ctcaaagaa 
      attgagcaa gattttgat aacattaaa atcgacgat 
      gagaatgtt gtcaatatc aaggaagtg agtggtgag 
      gaaatcagg gaaatccag tcgaaacca cagaccgtg 
      tacgcacct gttggtttc gtcgattta agggcgaga 
      aatgaaacg ataaaaaag ggtgtttcc cgagggttt 
      tttcacatt tttgccatt gtcctggct tatgtttta 
      atccacagt tactcgagt tatttggct gggtttttg 
      atcggtgtt tttgtgact tgtgtcttt tattatgtg 
      aaatttttg ctcgatact ccgtctcat agcccggca 
      attgccatc cagacgtgc aaagatatt ttacaagta 
      ccggcagtg aaggaattt caaccgttg actaaatac 
      gaaggttgg gttaatgaa tttcccgat aattacaat 
      cctcacact taccacatc agtcaaaca caatcggtg 
      tttttgcgt ttgcaaggc aatttactt cgtctgagt 
      cacacaaaa tcgaaagtt gccaaaagg gcgctttgg 
      aacgaaccg aaaatcaaa ccgattttc acacatcat 
      cgggtttat aatctaact ggggcacaa gtgagtttg 
      ttgccggtt ggtttggcg aagaagcgt cattggagt 
      aagaaatat ccgatttgt gtgactttg aacaaggat 
      caaatggat tttattgaa tcaccaatc gagaaaaaa 
      cgcagtcaa gggaataag aaaaagttt tcgattctg 
      gcacaaagg tttagtaag ttgacggaa gaaaacgaa 
      gattttgag tgtgattcg gagaattcg agggcgagt 
      agtccctca ccaccatcg ccagacgat tcagatgat 
      aaattgttg aacgaagcc ttggatgat gctttgaaa 
      gacgaggaa aacgaggtt ttactcgac gaagagttt 
      gagtcgatt ccggacgat tggagcggt ttgacacca 
      ccagatgaa agtccgacc caaacgaaa ctattcctt 
      ttcggacga actgaccga gagaaagaa gactggttc 
      aggagactt aaggcggcc acacataaa ggtgcaaac 
      ttaccgtca agtgacacc tgcgagatt ttgactgaa 
      agtgactac ataaagtat atgatgatt tttaataaa 
      attaaggtc gaaagtgac gtccagtgg atcaattct 
      ttggtcggt cgagtcttt ttcgattgt ttacgtgac 
      gataacttc actcaagga atcaaggat aaaatccag 
      agtaaatta tcgtcgatt aagcttcca tactttatt 
      gaaggtttg atggtgact gaactaaat ttgggtaaa 
      accccgcct ttgattcac aaagtaaca aaaccgagc 
      atagacgaa cgcgggttg tggatcgat ctcgacgta 
      acatacgaa ggtttagtc gttttaatt ttgcaaact 
      aaactcaac ttaatgaaa ctgaagcag ccacactct 
      aacgaaacg gtaaaagaa cgttcggcg atgttcaat 
      tccgatctt gaggattcc cctgaaagc acctctgat 
      gaggaaact ccgattttc cccaatcct ccggcactg 
      gattccggt caaagcggg agcaatacg agtaaaaaa 
      ttcatcaaa atggtagac cgcatagcg gaatccaag 
      tttttccaa gccgccacc gaataccgt tatataaaa 
      aaagcgatg gagggggtt tccaacacc gatttgcga 
      cttaaagtc gagattaag gaactggtg ggcactcta 
      gttttgaac attccgccg cctccgagt gaccgtgtt 
      tggattggc tttcgacca acccccgag ctggctctg 
      ggggcgcaa ccgatcgta ggcgaacgc aatatcacg 
      tttttgcgg attaccagt tggatcgag aagaagttg 
      cgtttggag ttccagaaa ttgttagtg attcccaac 
      atggaagac ttcattgta cctgtgatg agtccaaaa 
      ctaccggaa taacacgtg ataacacag tacagggca 
      cttttcgtc tagtctcaa tgtgaaaac aaccttgac 
      gttcgtaa