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Gene information
General information
TC005890 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5981491..5984213
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005890
    iBeetle screen iB_00954

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005890 RA
      MASRIRVTR TVIKRKASA SRVMRNAEK 
      IIEAVRDRP FLYDQAFQT FKHIGTKNR 
      NWMEVAKIV YGREDKVTV NKVKIQWKS 
      LRDAFVKQQ RKRQSSPKR KLRPYIYES 
      EMQFLLPHI LLKERDEES SPPLSNVEV 
      KTEDCSSLS LSGESPDAP PLAEESVWE 
      QQQQQHNAK TTHHPVDAF FYGIAQTVK 
      QLKPMTVAQ IKKSVANIV MDAEISEIE 
      EEGA
    CDS sequence
    • >TC005890 RA
      atggcgtcg cgcatccgc gttactcgt acggtgatc 
      aaacgcaaa gcgtccgcg agccgcgtc atgcgaaac 
      gcggaaaaa ataatcgaa gccgtccgt gacaggccc 
      tttctttat gaccaagca tttcaaaca tttaaacac 
      atcggaacg aaaaaccga aactggatg gaagttgcg 
      aaaattgtc tacggcaga gaggacaaa gttacagta 
      aataaagta aaaatccaa tggaagagt ttgcgtgac 
      gccttcgtg aaacaacag cgaaaacgg caaagttcc 
      ccaaagcgg aaactccgt ccttacatc tacgagagc 
      gaaatgcag ttcttactg cctcacatt ttgctgaaa 
      gagcgtgac gaggagagc agtcccccg ctttcgaac 
      gtggaagtg aaaacggag gattgctcg agtttgagt 
      ttatcgggg gaatccccg gacgcgccg ccgctggcg 
      gaagaaagc gtctgggag caacagcag cagcaacac 
      aacgcaaag acaacgcat catcccgtc gatgcgttc 
      ttctacgga atcgcccaa accgtgaaa caattaaaa 
      ccgatgaca gttgcgcaa atcaagaaa agtgtggcg 
      aacatcgtg atggatgcg gaaatcagc gagatcgag 
      gaggagggc gcatga
    mRNA sequence
    • >TC005890 RA
      cgcttaata acatttctt gagaaatta acttttcgc 
      gcttgtaat cctccgcca aattcaaaa attcaaaaa 
      acgtcaaat tcaaattca aaacatgtg cttaaaatt 
      agggattag ggattattt ttgcttaac gccgtattt 
      aatcataaa catttcgat ttctaaaaa cgtaaaaaa 
      ggaaaaaaa ttggtatgt ggtgctact tttagtgcg 
      gagtgtaaa aatggcgtc gcgcatccg cgttactcg 
      tacggtgat caaacgcaa agcgtccgc gagccgcgt 
      catgcgaaa cgcggaaaa aataatcga agccgtccg 
      tgacaggcc ctttcttta tgaccaagc atttcaaac 
      atttaaaca catcggaac gaaaaaccg aaactggat 
      ggaagttgc gaaaattgt ctacggcag agaggacaa 
      agttacagt aaataaagt aaaaatcca atggaagag 
      tttgcgtga cgccttcgt gaaacaaca gcgaaaacg 
      gcaaagttc cccaaagcg gaaactccg tccttacat 
      ctacgagag cgaaatgca gttcttact gcctcacat 
      tttgctgaa agagcgtga cgaggagag cagtccccc 
      gctttcgaa cgtggaagt gaaaacgga ggattgctc 
      gagtttgag tttatcggg ggaatcccc ggacgcgcc 
      gccgctggc ggaagaaag cgtctggga gcaacagca 
      gcagcaaca caacgcaaa gacaacgca tcatcccgt 
      cgatgcgtt cttctacgg aatcgccca aaccgtgaa 
      acaattaaa accgatgac agttgcgca aatcaagaa 
      aagtgtggc gaacatcgt gatggatgc ggaaatcag 
      cgagatcga ggaggaggg cgcatgaat aagggcccc 
      ctaactgcg tagttgtga tgacgtagc agcggcgtc 
      atgcttggc gggattctg agtatgaat gacctgtga 
      agagcgttg cccccgcct tcgcccccc tcgccctag 
      cgccccaac atccggtcc ggccttaca ccagcagat 
      tcggcggcg gcgacggcc accacacgc gccgcgttg 
      ccacatcgc accgttttc tcacatcca gaggcgaca 
      tttcgtatt tcccgccaa cagggccta cttacattg 
      aacgtcgct gcgacaatt aaggccgta acggtcgac 
      gtaacgaac gaatcttca tttatttcg aatgacagt 
      gccttagca cttaatttt tgttagttt tagccatag 
      aacaacttg cactttttg agtgatttt atttgtttt 
      ttactactt ttaaatgat tcaagagcc ttgactttt 
      atcataaat aaaaaaaaa tagaaaata tattaagaa 
      aatgttata taatcttga aatagctta tgctatttt 
      tgtagtgat gtatgttaa aattattaa tgtgaaaaa 
      taggaaatt gctgtatgt ataaaaaat ttagacatt 
      tttcaaata ctctgtaac caccaccac ttcagaata 
      tttctctct ttcataaaa aattaataa caaacgaat 
      attctgtat ggtgactgt ttgaaacca tgtgaagaa 
      atttttagt cctataatt tgcgaaatt tatgtaata 
      ataacgtca acttgtttc gagcactaa gactagtgt 
      ctgtacata atattggct gcctatatt ttttttgta 
      aatcgacaa ataaattat ttcatttaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY