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Gene information
General information
TC005869 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid53: 266131..267486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005869
    iBeetle screen iB_00950
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005869 RA
      MDRLLRLGG GMPGLSQAP PPSDAPVVD TAEQVYISS 
      LALLKMLKH GRAGVPMEV MGLMLGEFV DDYTVRVID 
      VFAMPQTGT GVSVEAVDP VFQAKMLDM LRQTGRPEM 
      VVGWYHSHP GFGCWLSGV DINTQQSFE ALSERAVAV 
      VVDPIQSVK GKVVIDAFR LINPNMMVL GQEPRQTTS 
      NLGHLQKPS VQALIHGLN RHYYSISIN YRKNELEQK 
      MLLNLHKKS WMDGLILAD YKNNCGINE KTVADVLEL 
      AKNYNKALE EEEKMTPEQ LAIKNVGKQ DPKRHLEEK 
      VDLLMTNNI VQCLGAMLD TVAFK
    CDS sequence
    • >TC005869 RA
      atggatcgt ttgcttcgt ttgggtggc ggaatgccg 
      ggcctgtct caggcgccc cctccgtcg gacgccccc 
      gtggtggac acggccgag caagtgtac atctcgtcg 
      ttggccctt ttgaagatg ttgaagcat ggccgggct 
      ggggtcccc atggaagtc atgggcctg atgttgggt 
      gaattcgtt gatgactac accgtgagg gtgatcgac 
      gtgttcgcg atgccccag accgggact ggggtcagt 
      gtggaggcc gtggacccc gtcttccag gccaaaatg 
      ctggacatg ttgcgccaa acgggccgc ccggagatg 
      gtcgtcggg tggtaccac tcccatccc gggttcggg 
      tgctggctg tcgggggtg gatatcaac acccagcag 
      tcgtttgaa gccctgagt gagcgggcg gtggccgtg 
      gtcgtggac cccatccag tcagtcaag ggcaaagtc 
      gtcatcgat gcctttagg ttaatcaac ccgaatatg 
      atggtcctg gggcaggag ccccgccag acgacgtcc 
      aatttgggg cacttgcag aagccgtca gttcaagcg 
      ctgattcac ggtttgaac agacactac tactctatt 
      agtataaac taccggaag aatgaatta gagcaaaag 
      atgttactc aatctgcat aagaagtcg tggatggat 
      ggcttaatc ctcgcagat tataaaaat aattgcggg 
      attaatgaa aaaacagtc gctgatgtt ttagaattg 
      gccaaaaat tataacaag gcgctggag gaagaggag 
      aagatgacg ccggagcag ttggcgatt aaaaatgtc 
      gggaaacag gatccgaaa cggcatctt gaggagaaa 
      gttgatttg ttgatgact aataatatt gtacagtgt 
      ttaggggcg atgttggac acggttgcg tttaaatga
    mRNA sequence
    • >TC005869 RA
      aatttactc aattgaaca atagtgccg ttcttaccc 
      agtccgtgg ggcccactt gcgtacttc tcgtgcagg 
      agattcttt ttttgcgcg tcgtttgcc atcgcagag 
      agttacaat accgttgag taaaatgga tcgtttgct 
      tcgtttggg tggcggaat gccgggcct gtctcaggc 
      gccccctcc gtcggacgc ccccgtggt ggacacggc 
      cgagcaagt gtacatctc gtcgttggc ccttttgaa 
      gatgttgaa gcatggccg ggctggggt ccccatgga 
      agtcatggg cctgatgtt gggtgaatt cgttgatga 
      ctacaccgt gagggtgat cgacgtgtt cgcgatgcc 
      ccagaccgg gactggggt cagtgtgga ggccgtgga 
      ccccgtctt ccaggccaa aatgctgga catgttgcg 
      ccaaacggg ccgcccgga gatggtcgt cgggtggta 
      ccactccca tcccgggtt cgggtgctg gctgtcggg 
      ggtggatat caacaccca gcagtcgtt tgaagccct 
      gagtgagcg ggcggtggc cgtggtcgt ggaccccat 
      ccagtcagt caagggcaa agtcgtcat cgatgcctt 
      taggttaat caacccgaa tatgatggt cctggggca 
      ggagccccg ccagacgac gtccaattt ggggcactt 
      gcagaagcc gtcagttca agcgctgat tcacggttt 
      gaacagaca ctactactc tattagtat aaactaccg 
      gaagaatga attagagca aaagatgtt actcaatct 
      gcataagaa gtcgtggat ggatggctt aatcctcgc 
      agattataa aaataattg cgggattaa tgaaaaaac 
      agtcgctga tgttttaga attggccaa aaattataa 
      caaggcgct ggaggaaga ggagaagat gacgccgga 
      gcagttggc gattaaaaa tgtcgggaa acaggatcc 
      gaaacggca tcttgagga gaaagttga tttgttgat 
      gactaataa tattgtaca gtgtttagg ggcgatgtt 
      ggacacggt tgcgtttaa atgaatttt tctcaaact 
      tgtcatcaa ttgaattgt gttttataa cttatttta 
      aatcaggat tttttctaa ttaaacgat ttacaattc 
      ggtgttttt tatttttat acgctactg tcattcacc 
      tccatataa caaaccaat tcttatcta aataaatca 
      gttaaaaaa gt