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Gene information
General information
TC005864 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6239212..6252340
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005864
    iBeetle screen iB_00948

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva dorsal partiallynot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005864 RA
      MPAHNSWTS MMSGKPDLT IKSSVIEHQ IQSVFARDS 
      VGTQFHGRP KSTYSIRRF FRKSHKQLR AVQIESLKN 
      QLIECCLTH IQDSYRVHL NRLKGLKIL ETFIHLVHI 
      SKISSELAT HMVWACLSC IHHFLLRLK SEGGLMQWL 
      RRKQARKSI RVFDNLMTF LIPFYYPVV FSAVITFFE 
      EANIWDIEY FCSNLLERL FLVVDEKRK TDSMMEIIS 
      LIENSTLTN ALTSRQILQ VLFDIEQSL KWDLMNDRL 
      LEKFLDMYY SSLTPEDNH EYNYESLEK GLETCLRAM 
      CANFGEKYL MLMIRWMLD MTTSEKISD ENLLNFGSL 
      LEHVTTLYT SKSLNRNLP EQVFPFIFK LIASKNLLY 
      SLLGNRVLH NLIDRCHNK LKFDAPRIF FRNSHYNIV 
      LNHYNSTDK LLFQRYREL LHKTFVESV IRHGVHKIH 
      LENIYISVV LFLVEIPCG YTAAAAVCL AMSIQEAAL 
      ESKSIQMTH AHRLHAAVV SIMSLVCYI HKASVFYDY 
      VDSIIDKRA NFAPHLNPP LKIKYKYAQ HHVLWNKPD 
      LFFEDWETR YGLWRCFKN RHKDIVVVH T
    • >TC005864 RB
      MPAHNSWTS MMSGKPDLT IKSSVIEHQ IQSVFARDS 
      VGTQFHGRP KSTYSIRRF FRKSHKQLR AVQIESLKN 
      QLIECCLTH IQDSYRVHL NRLKGLKIL ETFIHLVHI 
      SKISSELAT HMVWACLSC IHHFLLRLK SEGGLMQWL 
      RRKQARKSI RVFDNLMTF LIPFYYPVV FSAVITFFE 
      EANIWDIEY FCSNLLERL FLVVDEKRK TDSMMEIIS 
      LIENSTLTN ALTSRQILQ VLFDIEQSL KWDLMNDRL 
      LEKFLDMYY SSLTPEDNH EYNYESLEK GLETCLRAM 
      CANFGEKYL MLMIRWMLD MTTSEKISD ENLLNFGSL 
      LEHVTTLYT SKSLNRNLP EQVFPFIFK LIASKNLLY 
      SLLGNRVLH NLIDRCHNK LKFDAPRIF FRNSHYNIV 
      LNHYNSTDK LLFQRYREL LHKTFVESV IRHGVHKIH 
      LENIYISVV LFLVEIPCG YTAAAAVCL AMSIQEAAL 
      ESKSIQMTH AHRLHAAVV SIMSLVCYI HKASVFYDY 
      VDSIIDKRA NFAPHLNPP LKIKYKYAQ HHVLWNKPD 
      LFFEDWETR YGLWRCFKN RHKDIVVRH KASVFYDCV 
      DSIIDKRAN FAPHLNPPL KIKYKYAQH HVLWNKPDL 
      FFEDWETRY GLWRCFKNR HKDIVVVHT
    CDS sequence
    • >TC005864 RA
      atgccagcc cataattcc tggacctcg atgatgtcc 
      ggaaagccg gacctgacc atcaaatca tcagtgatc 
      gaacatcag atccagagt gtgttcgca agagactca 
      gtggggaca caatttcac ggtcgcccc aaatcgact 
      tacagcatc cgccgcttc ttccgcaaa tcgcacaag 
      caactcaga gccgtccag atcgaaagt ctcaaaaac 
      cagttgatc gaatgttgc ctcacacac atccaggac 
      tcgtaccgc gtccactta aaccgcctc aaagggctc 
      aagatcctg gaaaccttc atccacttg gtccacatc 
      tcgaagatc agctcggaa ctggccaca cacatggtg 
      tgggcctgt ctcagctgc atccaccat ttcctgctg 
      cggctcaag tcggaaggg gggctcatg cagtggctg 
      cgaaggaag caggcacgc aagtcgatt cgagtcttc 
      gacaattta atgacgttc cttataccc ttctactat 
      cctgtggtt ttctcagcg gttataact tttttcgaa 
      gaggccaac atctgggat attgagtac ttttgctcg 
      aatttactc gaacgcttg tttttggtt gtcgacgag 
      aaacgcaag accgattcc atgatggag attatttcc 
      ctgattgaa aactcgacc ctcaccaac gcgctcaca 
      tctcgccaa atcctccaa gtgttattc gacattgag 
      caaagttta aaatgggat ttgatgaac gaccgcctc 
      cttgaaaaa ttcctcgac atgtactac agcagttta 
      acacctgaa gacaatcac gaatacaac tacgagtca 
      ctcgagaag ggcctcgag acgtgccta agggccatg 
      tgtgccaac tttggcgaa aaatatcta atgctcatg 
      attaggtgg atgttggac atgacaact tcggaaaaa 
      atctccgac gaaaacctg ctcaatttc ggcagtttg 
      ctggaacac gtcaccacc ctgtacacc tccaagagc 
      ctgaaccgg aatttgccc gagcaggtg ttccctttc 
      atcttcaag ctgattgcg tcgaaaaat ctgctctat 
      agccttctc ggcaaccgg gttttgcac aacttgatc 
      gaccgctgc cacaataag ctaaagttc gacgcgcct 
      cgcattttt ttcagaaac tcgcactac aatatcgtg 
      ctgaatcac tacaattcc accgataag ctgcttttc 
      cagcgctat cgcgaattg ttgcacaag actttcgtc 
      gagtccgtt atcagacac ggagtccac aaaattcat 
      ttggagaac atctacatt tctgtcgtg ctgttcctg 
      gtggagatt ccctgcggg tacacggct gccgcagcc 
      gtttgtctg gcgatgtcc atccaagag gcggcgctc 
      gagagcaag agcatccaa atgactcac gcacaccgc 
      ctccatgcg gctgtggtc tcgataatg tccctcgtt 
      tgttacatc cataaagct tcagtgttt tatgattac 
      gttgattcg attattgat aagagggcg aattttgcg 
      ccacatttg aatccgccg ctgaaaatt aagtacaag 
      tatgcgcag catcacgtg ttgtggaac aagccggac 
      ttgttcttt gaggattgg gagacgagg tatgggctc 
      tggaggtgc ttcaagaac aggcataag gatattgtt 
      gtggtgcac acctaa
    • >TC005864 RB
      atgccagcc cataattcc tggacctcg atgatgtcc 
      ggaaagccg gacctgacc atcaaatca tcagtgatc 
      gaacatcag atccagagt gtgttcgca agagactca 
      gtggggaca caatttcac ggtcgcccc aaatcgact 
      tacagcatc cgccgcttc ttccgcaaa tcgcacaag 
      caactcaga gccgtccag atcgaaagt ctcaaaaac 
      cagttgatc gaatgttgc ctcacacac atccaggac 
      tcgtaccgc gtccactta aaccgcctc aaagggctc 
      aagatcctg gaaaccttc atccacttg gtccacatc 
      tcgaagatc agctcggaa ctggccaca cacatggtg 
      tgggcctgt ctcagctgc atccaccat ttcctgctg 
      cggctcaag tcggaaggg gggctcatg cagtggctg 
      cgaaggaag caggcacgc aagtcgatt cgagtcttc 
      gacaattta atgacgttc cttataccc ttctactat 
      cctgtggtt ttctcagcg gttataact tttttcgaa 
      gaggccaac atctgggat attgagtac ttttgctcg 
      aatttactc gaacgcttg tttttggtt gtcgacgag 
      aaacgcaag accgattcc atgatggag attatttcc 
      ctgattgaa aactcgacc ctcaccaac gcgctcaca 
      tctcgccaa atcctccaa gtgttattc gacattgag 
      caaagttta aaatgggat ttgatgaac gaccgcctc 
      cttgaaaaa ttcctcgac atgtactac agcagttta 
      acacctgaa gacaatcac gaatacaac tacgagtca 
      ctcgagaag ggcctcgag acgtgccta agggccatg 
      tgtgccaac tttggcgaa aaatatcta atgctcatg 
      attaggtgg atgttggac atgacaact tcggaaaaa 
      atctccgac gaaaacctg ctcaatttc ggcagtttg 
      ctggaacac gtcaccacc ctgtacacc tccaagagc 
      ctgaaccgg aatttgccc gagcaggtg ttccctttc 
      atcttcaag ctgattgcg tcgaaaaat ctgctctat 
      agccttctc ggcaaccgg gttttgcac aacttgatc 
      gaccgctgc cacaataag ctaaagttc gacgcgcct 
      cgcattttt ttcagaaac tcgcactac aatatcgtg 
      ctgaatcac tacaattcc accgataag ctgcttttc 
      cagcgctat cgcgaattg ttgcacaag actttcgtc 
      gagtccgtt atcagacac ggagtccac aaaattcat 
      ttggagaac atctacatt tctgtcgtg ctgttcctg 
      gtggagatt ccctgcggg tacacggct gccgcagcc 
      gtttgtctg gcgatgtcc atccaagag gcggcgctc 
      gagagcaag agcatccaa atgactcac gcacaccgc 
      ctccatgcg gctgtggtc tcgataatg tccctcgtt 
      tgttacatc cataaagct tcagtgttt tatgattac 
      gttgattcg attattgat aagagggcg aattttgcg 
      ccacatttg aatccgccg ctgaaaatt aagtacaag 
      tatgcgcag catcacgtg ttgtggaac aagccggac 
      ttgttcttt gaggattgg gagacgagg tatgggctc 
      tggaggtgc ttcaagaac aggcataag gatatcgtt 
      gtgcgacat aaagcttca gtgttttat gattgcgtt 
      gattcgatt attgataag agggcgaat tttgcgcca 
      catttgaat ccgccgctg aaaattaag tacaagtat 
      gcgcagcat cacgtgttg tggaacaag ccggacttg 
      ttctttgag gattgggag acgaggtat gggctctgg 
      aggtgcttc aagaacagg cataaggat attgttgtg 
      gtgcacacc taa
    mRNA sequence
    • >TC005864 RA
      actaataac aaaaagagt ccctaaaaa tttattaat 
      atttgagtt aaaattggt atagtgttt tgttggttg 
      cataaaatg tgtacttaa tgctgttgg ggtagacag 
      ttgacacag ttgacactt tcggcaaaa tggccacag 
      gctaaaatc tctcgtaac tcttgacca tgccagccc 
      ataattcct ggacctcga tgatgtccg gaaagccgg 
      acctgacca tcaaatcat cagtgatcg aacatcaga 
      tccagagtg tgttcgcaa gagactcag tggggacac 
      aatttcacg gtcgcccca aatcgactt acagcatcc 
      gccgcttct tccgcaaat cgcacaagc aactcagag 
      ccgtccaga tcgaaagtc tcaaaaacc agttgatcg 
      aatgttgcc tcacacaca tccaggact cgtaccgcg 
      tccacttaa accgcctca aagggctca agatcctgg 
      aaaccttca tccacttgg tccacatct cgaagatca 
      gctcggaac tggccacac acatggtgt gggcctgtc 
      tcagctgca tccaccatt tcctgctgc ggctcaagt 
      cggaagggg ggctcatgc agtggctgc gaaggaagc 
      aggcacgca agtcgattc gagtcttcg acaatttaa 
      tgacgttcc ttataccct tctactatc ctgtggttt 
      tctcagcgg ttataactt ttttcgaag aggccaaca 
      tctgggata ttgagtact tttgctcga atttactcg 
      aacgcttgt ttttggttg tcgacgaga aacgcaaga 
      ccgattcca tgatggaga ttatttccc tgattgaaa 
      actcgaccc tcaccaacg cgctcacat ctcgccaaa 
      tcctccaag tgttattcg acattgagc aaagtttaa 
      aatgggatt tgatgaacg accgcctcc ttgaaaaat 
      tcctcgaca tgtactaca gcagtttaa cacctgaag 
      acaatcacg aatacaact acgagtcac tcgagaagg 
      gcctcgaga cgtgcctaa gggccatgt gtgccaact 
      ttggcgaaa aatatctaa tgctcatga ttaggtgga 
      tgttggaca tgacaactt cggaaaaaa tctccgacg 
      aaaacctgc tcaatttcg gcagtttgc tggaacacg 
      tcaccaccc tgtacacct ccaagagcc tgaaccgga 
      atttgcccg agcaggtgt tccctttca tcttcaagc 
      tgattgcgt cgaaaaatc tgctctata gccttctcg 
      gcaaccggg ttttgcaca acttgatcg accgctgcc 
      acaataagc taaagttcg acgcgcctc gcatttttt 
      tcagaaact cgcactaca atatcgtgc tgaatcact 
      acaattcca ccgataagc tgcttttcc agcgctatc 
      gcgaattgt tgcacaaga ctttcgtcg agtccgtta 
      tcagacacg gagtccaca aaattcatt tggagaaca 
      tctacattt ctgtcgtgc tgttcctgg tggagattc 
      cctgcgggt acacggctg ccgcagccg tttgtctgg 
      cgatgtcca tccaagagg cggcgctcg agagcaaga 
      gcatccaaa tgactcacg cacaccgcc tccatgcgg 
      ctgtggtct cgataatgt ccctcgttt gttacatcc 
      ataaagctt cagtgtttt atgattacg ttgattcga 
      ttattgata agagggcga attttgcgc cacatttga 
      atccgccgc tgaaaatta agtacaagt atgcgcagc 
      atcacgtgt tgtggaaca agccggact tgttctttg 
      aggattggg agacgaggt atgggctct ggaggtgct 
      tcaagaaca ggcataagg atattgttg tggtgcaca 
      cctaagaga tgatcagat ggactctgg gatttgagg 
      aatttttca atggaagat ggacacagt cgacttgag 
      actaggaaa tgcttacac atgcatttt ttacggaat 
      aaatggtca aaataaaaa ttatgattc gag
    • >TC005864 RB
      actaataac aaaaagagt ccctaaaaa tttattaat 
      atttgagtt aaaattggt atagtgttt tgttggttg 
      cataaaatg tgtacttaa tgctgttgg ggtagacag 
      ttgacacag ttgacactt tcggcaaaa tggccacag 
      gctaaaatc tctcgtaac tcttgacca tgccagccc 
      ataattcct ggacctcga tgatgtccg gaaagccgg 
      acctgacca tcaaatcat cagtgatcg aacatcaga 
      tccagagtg tgttcgcaa gagactcag tggggacac 
      aatttcacg gtcgcccca aatcgactt acagcatcc 
      gccgcttct tccgcaaat cgcacaagc aactcagag 
      ccgtccaga tcgaaagtc tcaaaaacc agttgatcg 
      aatgttgcc tcacacaca tccaggact cgtaccgcg 
      tccacttaa accgcctca aagggctca agatcctgg 
      aaaccttca tccacttgg tccacatct cgaagatca 
      gctcggaac tggccacac acatggtgt gggcctgtc 
      tcagctgca tccaccatt tcctgctgc ggctcaagt 
      cggaagggg ggctcatgc agtggctgc gaaggaagc 
      aggcacgca agtcgattc gagtcttcg acaatttaa 
      tgacgttcc ttataccct tctactatc ctgtggttt 
      tctcagcgg ttataactt ttttcgaag aggccaaca 
      tctgggata ttgagtact tttgctcga atttactcg 
      aacgcttgt ttttggttg tcgacgaga aacgcaaga 
      ccgattcca tgatggaga ttatttccc tgattgaaa 
      actcgaccc tcaccaacg cgctcacat ctcgccaaa 
      tcctccaag tgttattcg acattgagc aaagtttaa 
      aatgggatt tgatgaacg accgcctcc ttgaaaaat 
      tcctcgaca tgtactaca gcagtttaa cacctgaag 
      acaatcacg aatacaact acgagtcac tcgagaagg 
      gcctcgaga cgtgcctaa gggccatgt gtgccaact 
      ttggcgaaa aatatctaa tgctcatga ttaggtgga 
      tgttggaca tgacaactt cggaaaaaa tctccgacg 
      aaaacctgc tcaatttcg gcagtttgc tggaacacg 
      tcaccaccc tgtacacct ccaagagcc tgaaccgga 
      atttgcccg agcaggtgt tccctttca tcttcaagc 
      tgattgcgt cgaaaaatc tgctctata gccttctcg 
      gcaaccggg ttttgcaca acttgatcg accgctgcc 
      acaataagc taaagttcg acgcgcctc gcatttttt 
      tcagaaact cgcactaca atatcgtgc tgaatcact 
      acaattcca ccgataagc tgcttttcc agcgctatc 
      gcgaattgt tgcacaaga ctttcgtcg agtccgtta 
      tcagacacg gagtccaca aaattcatt tggagaaca 
      tctacattt ctgtcgtgc tgttcctgg tggagattc 
      cctgcgggt acacggctg ccgcagccg tttgtctgg 
      cgatgtcca tccaagagg cggcgctcg agagcaaga 
      gcatccaaa tgactcacg cacaccgcc tccatgcgg 
      ctgtggtct cgataatgt ccctcgttt gttacatcc 
      ataaagctt cagtgtttt atgattacg ttgattcga 
      ttattgata agagggcga attttgcgc cacatttga 
      atccgccgc tgaaaatta agtacaagt atgcgcagc 
      atcacgtgt tgtggaaca agccggact tgttctttg 
      aggattggg agacgaggt atgggctct ggaggtgct 
      tcaagaaca ggcataagg atatcgttg tgcgacata 
      aagcttcag tgttttatg attgcgttg attcgatta 
      ttgataaga gggcgaatt ttgcgccac atttgaatc 
      cgccgctga aaattaagt acaagtatg cgcagcatc 
      acgtgttgt ggaacaagc cggacttgt tctttgagg 
      attgggaga cgaggtatg ggctctgga ggtgcttca 
      agaacaggc ataaggata ttgttgtgg tgcacacct 
      aagagatga tcagatgga ctctgggat ttgaggaat 
      ttttcaatg gaagatgga cacagtcga cttgagact 
      aggaaatgc ttacacatg catttttta cggaataaa 
      tggtcaaaa taaaaatta tgattcgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY