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Gene information
General information
TC005858 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6329925..6353342
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005858
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    leg musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    leg musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    leg musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    labrum potentially size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    head size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs partially size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    pygopodium partially size irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005858 RA
      MFPVAICIA FLFLQIQAM PTIITAHPP SEERFSDDW 
      DYKIGHDTV PVLQQFQKN HTLPPVANE TARLLWELD 
      QEKSTHLPQ FVDQALKLL NLKQVAFEI ENSVMSKVS 
      CTACKAGAG LLQHYIKTG KSEKEIKKT IYQFCVNLK 
      IQTPRVCEG ITELFAGEV IYVLGKVTI GPDEVCSFV 
      IGDACGDVY NPLHEWEVM FPPVPKPAI VEQKIPEIN 
      APTFKVLHL SDTHYDPYY HEGSNADCS EPLCCRLTN 
      GLASTKEQA AGKWGDYRK CDTPKITVD NMLQHIQET 
      HPDIDYILW TGDLPPHDI WNQTREENL KIIKETVKQ 
      MSDMFPGAP IFPALGNHE SAPVNSFPP PFIDNPDNS 
      ISWLYDELD VQWRKWLPS SVSTTVRRG AFYSVLVRP 
      GFRLISLNT NYCNNKNWW LLLNSTDPA TELQWFIYE 
      LQSAEFNNE KVHVIGHIP PGHSDCLKV WSRNYYAIV 
      NRYEATITA QFFGHTHFD EFEVFYDHK DLTRANNVA 
      YVGPSVSPY YDLNPGYRI YYVDGDHDK STRSVIDHE 
      SWTMNLREA NLYGYPIWF KLYSARQAF GMDALRPSD 
      WDALIDKMT NDPKLFELF YKYYYKASP VRPTCDMSC 
      KKKILCDLH SGRSHDRKN LCQSIESRI DSTANTTWK 
      EWFYNTISV SMSVLWSIP RLTIQLPKY VLGLG
    • >TC005858 RB
      MFPVAICIA FLFLQIQAM PTIITAHPP SEERFSDDW 
      DYKIGHDTV PVLQQFQKN HTLPPVANE TARLLWELD 
      QEKSTHLPQ FVDQALKLL NLKQVAFEI ENSVMSKVS 
      CTACKAGAG LLQHYIKTG KSEKEIKKT IYQFCVNLK 
      IQTPRVCEG ITELFAGEV IYVLGKVTI GPDEVCSFV 
      IGDACGDVY NPLHEWEVM FPPVPKPAI VEQKIPEIN 
      APTFKVLHL SDTHYDPYY HEGSNADCS EPLCCRLTN 
      GLASTKEQA AGKWGDYRK CDTPKITVD NMLQHIQET 
      HPDIDYILW TGDLPPHDI WNQTREENL KIIKETVKQ 
      MSDMFPGAP IFPALGNHE SAPVNSFPP PFIDNPDNS 
      ISWLYDELD VQWRKWLPS SVSTTVRRG AFYSVLVRP 
      GFRLISLNT NYCNNKNWW LLLNSTDPA TELQWFIYE 
      LQSAEFNNE KVHVIGHIP PGHSDCLKV WSRNYYAIV 
      NRYEATITA QFFGHTHFD EFEVFYDHK DLTRANNVA 
      YVGPSVSPY YDLNPGYRI YYVDGDHDK STRSVIDHE 
      SWTMNLREA NLYGYPIWF KLYSARQAF GMDALRPSD 
      WDALIDKMT NDPKLFELF YKYYYKASP VRPTCDMSC 
      KKKILCDLH SGRSHDRKN LCQSIESRI DSTANTTWK 
      EWFYNTISV SVV
    CDS sequence
    • >TC005858 RA
      atgttccca gtggcaata tgtatcgct ttcctcttt 
      ttacaaatc caagcaatg ccaacaatt ataacagca 
      caccctccc tcagaagag cgcttcagc gatgactgg 
      gactacaaa atcggacat gatacggtt cctgttttg 
      caacagttt cagaaaaat cacacactt ccacctgtg 
      gctaatgag actgctcga cttttgtgg gagcttgat 
      caggaaaaa tccacacat ttgccgcag tttgttgat 
      caagccttg aaattgctg aatttgaaa caagtggct 
      ttcgaaatt gaaaattca gtgatgtcg aaagtgtca 
      tgtaccgca tgtaaagca ggtgccggc ttgttacag 
      cactacatt aaaaccggc aaatcggag aaagaaatt 
      aaaaaaaca atttaccaa ttttgcgtc aacttaaaa 
      atccaaaca cctcgcgtt tgcgaagga attactgaa 
      cttttcgct ggggaagta atctatgtt ttgggtaaa 
      gttaccatc ggacctgac gaagtttgt agttttgta 
      atcggagac gcttgtggt gatgtttat aatccattg 
      catgaatgg gaagttatg tttccgcca gttccaaaa 
      ccagcgatt gtcgaacaa aaaattcca gaaataaat 
      gcgccaact tttaaagtt ttgcatttg tcggacacc 
      cattacgat ccatattac catgaaggg agcaatgct 
      gattgtagt gaaccgctg tgctgtagg ttgaccaat 
      gggttggcc agtaccaag gaacaggca gctgggaaa 
      tggggcgac taccgcaag tgcgacaca ccgaaaatc 
      accgtcgac aatatgctc cagcacatc caggagacc 
      catcccgac atcgactac atcctctgg accggtgat 
      ctgccaccg cacgacatc tggaaccaa acgcgcgaa 
      gaaaactta aaaattatc aaagaaaca gtcaaacaa 
      atgtctgac atgttccca ggcgctcca attttccca 
      gctttggga aaccacgaa tcggcgccg gttaatagt 
      ttcccgcca ccgtttatc gataatcca gacaactcc 
      atctcgtgg ttgtacgac gagttggat gtacagtgg 
      cggaagtgg ttgccgagt tctgtgagc acgactgtt 
      aggagagga gcgttttat tcggttttg gtcagacct 
      ggatttaga ttgatttcg ctgaatacg aattattgt 
      aataataag aactggtgg ctgttgttg aatagtaca 
      gacccggcc actgagctc cagtggttt atttatgag 
      ttgcagtcg gcggagttt aacaacgag aaagtccac 
      gttattggt cacattcct ccgggccat tccgactgt 
      ttgaaagta tggtcgcgg aattattac gcaattgtt 
      aacagatat gaggccaca atcactgct cagttcttc 
      ggacataca catttcgat gaatttgaa gtcttctac 
      gaccataaa gatttaacc agagcaaac aatgtagct 
      tatgtgggc ccttctgtt tcaccgtac tatgactta 
      aatcctggc tataggatt tattatgtg gatggcgat 
      cacgacaaa tctacaaga tcggtgatt gatcacgag 
      tcttggact atgaatcta cgagaagct aatttatat 
      ggttatccg atttggttt aaattatac tcagcaaga 
      caagctttt ggaatggat gcacttcga ccaagtgac 
      tgggacgct ttgattgac aaaatgacg aatgatcca 
      aaattgttc gaactgttt tacaaatat tactacaag 
      gcgagtccc gtgagaccc acgtgtgac atgtcatgc 
      aagaaaaaa attctttgt gatctacat tcaggcaga 
      tcacacgac agaaaaaat ctctgtcag tcaattgaa 
      tctcgaata gattcaaca gcaaacacc acgtggaag 
      gagtggttt tacaacaca atatccgta tcgatgtcc 
      gtcttgtgg tccatccca cggctcaca attcaacta 
      cctaagtat gttctgggt cttgggtaa
    • >TC005858 RB
      atgttccca gtggcaata tgtatcgct ttcctcttt 
      ttacaaatc caagcaatg ccaacaatt ataacagca 
      caccctccc tcagaagag cgcttcagc gatgactgg 
      gactacaaa atcggacat gatacggtt cctgttttg 
      caacagttt cagaaaaat cacacactt ccacctgtg 
      gctaatgag actgctcga cttttgtgg gagcttgat 
      caggaaaaa tccacacat ttgccgcag tttgttgat 
      caagccttg aaattgctg aatttgaaa caagtggct 
      ttcgaaatt gaaaattca gtgatgtcg aaagtgtca 
      tgtaccgca tgtaaagca ggtgccggc ttgttacag 
      cactacatt aaaaccggc aaatcggag aaagaaatt 
      aaaaaaaca atttaccaa ttttgcgtc aacttaaaa 
      atccaaaca cctcgcgtt tgcgaagga attactgaa 
      cttttcgct ggggaagta atctatgtt ttgggtaaa 
      gttaccatc ggacctgac gaagtttgt agttttgta 
      atcggagac gcttgtggt gatgtttat aatccattg 
      catgaatgg gaagttatg tttccgcca gttccaaaa 
      ccagcgatt gtcgaacaa aaaattcca gaaataaat 
      gcgccaact tttaaagtt ttgcatttg tcggacacc 
      cattacgat ccatattac catgaaggg agcaatgct 
      gattgtagt gaaccgctg tgctgtagg ttgaccaat 
      gggttggcc agtaccaag gaacaggca gctgggaaa 
      tggggcgac taccgcaag tgcgacaca ccgaaaatc 
      accgtcgac aatatgctc cagcacatc caggagacc 
      catcccgac atcgactac atcctctgg accggtgat 
      ctgccaccg cacgacatc tggaaccaa acgcgcgaa 
      gaaaactta aaaattatc aaagaaaca gtcaaacaa 
      atgtctgac atgttccca ggcgctcca attttccca 
      gctttggga aaccacgaa tcggcgccg gttaatagt 
      ttcccgcca ccgtttatc gataatcca gacaactcc 
      atctcgtgg ttgtacgac gagttggat gtacagtgg 
      cggaagtgg ttgccgagt tctgtgagc acgactgtt 
      aggagagga gcgttttat tcggttttg gtcagacct 
      ggatttaga ttgatttcg ctgaatacg aattattgt 
      aataataag aactggtgg ctgttgttg aatagtaca 
      gacccggcc actgagctc cagtggttt atttatgag 
      ttgcagtcg gcggagttt aacaacgag aaagtccac 
      gttattggt cacattcct ccgggccat tccgactgt 
      ttgaaagta tggtcgcgg aattattac gcaattgtt 
      aacagatat gaggccaca atcactgct cagttcttc 
      ggacataca catttcgat gaatttgaa gtcttctac 
      gaccataaa gatttaacc agagcaaac aatgtagct 
      tatgtgggc ccttctgtt tcaccgtac tatgactta 
      aatcctggc tataggatt tattatgtg gatggcgat 
      cacgacaaa tctacaaga tcggtgatt gatcacgag 
      tcttggact atgaatcta cgagaagct aatttatat 
      ggttatccg atttggttt aaattatac tcagcaaga 
      caagctttt ggaatggat gcacttcga ccaagtgac 
      tgggacgct ttgattgac aaaatgacg aatgatcca 
      aaattgttc gaactgttt tacaaatat tactacaag 
      gcgagtccc gtgagaccc acgtgtgac atgtcatgc 
      aagaaaaaa attctttgt gatctacat tcaggcaga 
      tcacacgac agaaaaaat ctctgtcag tcaattgaa 
      tctcgaata gattcaaca gcaaacacc acgtggaag 
      gagtggttt tacaacaca atatccgta tcagtggtg 
      tag
    mRNA sequence
    • >TC005858 RA
      tatgtcgcg ttcagtctc cgactcgca ttcgcaccg 
      aacggatgg tcaagacac ggaggccgt tcgggtttt 
      attggttat gtgttggaa caagtaaat tggactttg 
      tggcgtttc ttcctccct ccgatatgt tcccagtgg 
      caatatgta tcgctttcc tctttttac aaatccaag 
      caatgccaa caattataa cagcacacc ctccctcag 
      aagagcgct tcagcgatg actgggact acaaaatcg 
      gacatgata cggttcctg ttttgcaac agtttcaga 
      aaaatcaca cacttccac ctgtggcta atgagactg 
      ctcgacttt tgtgggagc ttgatcagg aaaaatcca 
      cacatttgc cgcagtttg ttgatcaag ccttgaaat 
      tgctgaatt tgaaacaag tggctttcg aaattgaaa 
      attcagtga tgtcgaaag tgtcatgta ccgcatgta 
      aagcaggtg ccggcttgt tacagcact acattaaaa 
      ccggcaaat cggagaaag aaattaaaa aaacaattt 
      accaatttt gcgtcaact taaaaatcc aaacacctc 
      gcgtttgcg aaggaatta ctgaacttt tcgctgggg 
      aagtaatct atgttttgg gtaaagtta ccatcggac 
      ctgacgaag tttgtagtt ttgtaatcg gagacgctt 
      gtggtgatg tttataatc cattgcatg aatgggaag 
      ttatgtttc cgccagttc caaaaccag cgattgtcg 
      aacaaaaaa ttccagaaa taaatgcgc caactttta 
      aagttttgc atttgtcgg acacccatt acgatccat 
      attaccatg aagggagca atgctgatt gtagtgaac 
      cgctgtgct gtaggttga ccaatgggt tggccagta 
      ccaaggaac aggcagctg ggaaatggg gcgactacc 
      gcaagtgcg acacaccga aaatcaccg tcgacaata 
      tgctccagc acatccagg agacccatc ccgacatcg 
      actacatcc tctggaccg gtgatctgc caccgcacg 
      acatctgga accaaacgc gcgaagaaa acttaaaaa 
      ttatcaaag aaacagtca aacaaatgt ctgacatgt 
      tcccaggcg ctccaattt tcccagctt tgggaaacc 
      acgaatcgg cgccggtta atagtttcc cgccaccgt 
      ttatcgata atccagaca actccatct cgtggttgt 
      acgacgagt tggatgtac agtggcgga agtggttgc 
      cgagttctg tgagcacga ctgttagga gaggagcgt 
      tttattcgg ttttggtca gacctggat ttagattga 
      tttcgctga atacgaatt attgtaata ataagaact 
      ggtggctgt tgttgaata gtacagacc cggccactg 
      agctccagt ggtttattt atgagttgc agtcggcgg 
      agtttaaca acgagaaag tccacgtta ttggtcaca 
      ttcctccgg gccattccg actgtttga aagtatggt 
      cgcggaatt attacgcaa ttgttaaca gatatgagg 
      ccacaatca ctgctcagt tcttcggac atacacatt 
      tcgatgaat ttgaagtct tctacgacc ataaagatt 
      taaccagag caaacaatg tagcttatg tgggccctt 
      ctgtttcac cgtactatg acttaaatc ctggctata 
      ggatttatt atgtggatg gcgatcacg acaaatcta 
      caagatcgg tgattgatc acgagtctt ggactatga 
      atctacgag aagctaatt tatatggtt atccgattt 
      ggtttaaat tatactcag caagacaag cttttggaa 
      tggatgcac ttcgaccaa gtgactggg acgctttga 
      ttgacaaaa tgacgaatg atccaaaat tgttcgaac 
      tgttttaca aatattact acaaggcga gtcccgtga 
      gacccacgt gtgacatgt catgcaaga aaaaaattc 
      tttgtgatc tacattcag gcagatcac acgacagaa 
      aaaatctct gtcagtcaa ttgaatctc gaatagatt 
      caacagcaa acaccacgt ggaaggagt ggttttaca 
      acacaatat ccgtatcga tgtccgtct tgtggtcca 
      tcccacggc tcacaattc aactaccta agtatgttc 
      tgggtcttg ggtaacgcc taccattcg tttcggaag 
      tttattaga ttagttttc attatttat aaaaactac 
      caactccta ctctttcca atctcgttt tattttatt 
      tttttttaa tcaaggctg tgtattttt gatgaaatc 
      aaaaaatac cgacgatct ttttattta taattattt 
      aaagagagt gtaatgata atgaattta ccataaatg 
      tactaatat agttattta tttatttgg tgcggcatg 
      attttaaga tgcaacttt tttctcgat ttggattat 
      taaagttac gtcttttaa tgatgtttc cgaacgtgg 
      taggtgaat aggtttgta tttagtgat gtacaaatg 
      aaattgtta ttatttttg caataaaac aaatttaac 
      cta
    • >TC005858 RB
      tatgtcgcg ttcagtctc cgactcgca ttcgcaccg 
      aacggatgg tcaagacac ggaggccgt tcgggtttt 
      attggttat gtgttggaa caagtaaat tggactttg 
      tggcgtttc ttcctccct ccgatatgt tcccagtgg 
      caatatgta tcgctttcc tctttttac aaatccaag 
      caatgccaa caattataa cagcacacc ctccctcag 
      aagagcgct tcagcgatg actgggact acaaaatcg 
      gacatgata cggttcctg ttttgcaac agtttcaga 
      aaaatcaca cacttccac ctgtggcta atgagactg 
      ctcgacttt tgtgggagc ttgatcagg aaaaatcca 
      cacatttgc cgcagtttg ttgatcaag ccttgaaat 
      tgctgaatt tgaaacaag tggctttcg aaattgaaa 
      attcagtga tgtcgaaag tgtcatgta ccgcatgta 
      aagcaggtg ccggcttgt tacagcact acattaaaa 
      ccggcaaat cggagaaag aaattaaaa aaacaattt 
      accaatttt gcgtcaact taaaaatcc aaacacctc 
      gcgtttgcg aaggaatta ctgaacttt tcgctgggg 
      aagtaatct atgttttgg gtaaagtta ccatcggac 
      ctgacgaag tttgtagtt ttgtaatcg gagacgctt 
      gtggtgatg tttataatc cattgcatg aatgggaag 
      ttatgtttc cgccagttc caaaaccag cgattgtcg 
      aacaaaaaa ttccagaaa taaatgcgc caactttta 
      aagttttgc atttgtcgg acacccatt acgatccat 
      attaccatg aagggagca atgctgatt gtagtgaac 
      cgctgtgct gtaggttga ccaatgggt tggccagta 
      ccaaggaac aggcagctg ggaaatggg gcgactacc 
      gcaagtgcg acacaccga aaatcaccg tcgacaata 
      tgctccagc acatccagg agacccatc ccgacatcg 
      actacatcc tctggaccg gtgatctgc caccgcacg 
      acatctgga accaaacgc gcgaagaaa acttaaaaa 
      ttatcaaag aaacagtca aacaaatgt ctgacatgt 
      tcccaggcg ctccaattt tcccagctt tgggaaacc 
      acgaatcgg cgccggtta atagtttcc cgccaccgt 
      ttatcgata atccagaca actccatct cgtggttgt 
      acgacgagt tggatgtac agtggcgga agtggttgc 
      cgagttctg tgagcacga ctgttagga gaggagcgt 
      tttattcgg ttttggtca gacctggat ttagattga 
      tttcgctga atacgaatt attgtaata ataagaact 
      ggtggctgt tgttgaata gtacagacc cggccactg 
      agctccagt ggtttattt atgagttgc agtcggcgg 
      agtttaaca acgagaaag tccacgtta ttggtcaca 
      ttcctccgg gccattccg actgtttga aagtatggt 
      cgcggaatt attacgcaa ttgttaaca gatatgagg 
      ccacaatca ctgctcagt tcttcggac atacacatt 
      tcgatgaat ttgaagtct tctacgacc ataaagatt 
      taaccagag caaacaatg tagcttatg tgggccctt 
      ctgtttcac cgtactatg acttaaatc ctggctata 
      ggatttatt atgtggatg gcgatcacg acaaatcta 
      caagatcgg tgattgatc acgagtctt ggactatga 
      atctacgag aagctaatt tatatggtt atccgattt 
      ggtttaaat tatactcag caagacaag cttttggaa 
      tggatgcac ttcgaccaa gtgactggg acgctttga 
      ttgacaaaa tgacgaatg atccaaaat tgttcgaac 
      tgttttaca aatattact acaaggcga gtcccgtga 
      gacccacgt gtgacatgt catgcaaga aaaaaattc 
      tttgtgatc tacattcag gcagatcac acgacagaa 
      aaaatctct gtcagtcaa ttgaatctc gaatagatt 
      caacagcaa acaccacgt ggaaggagt ggttttaca 
      acacaatat ccgtatcag tggtgtagg gacgacttc 
      atatgactt caatcactt tgagggatt ttcttaggt 
      aaggttata ttttttcag gtggtggtg actaattaa 
      ttttcaatt gatttctgt taaaaaaaa ttgttaaat 
      aataaaata taaagtatg tatcaattg ttgttggca 
      ccgtaattt caagtcaca gctcactct aatcagaaa 
      ttaattgaa gactcgtta caataaatt atttttttt 
      caattttgt ttttgttta attaggatg tccgtcttg 
      tggtccatc ccacggctc acaattcaa ctacctaag 
      tatgttctg ggtcttggg taacgccta ccattcgtt 
      tcggaagtt tattagatt agttttcat tatttataa 
      aaactacca actcctact ctttccaat ctcgtttta 
      ttttatttt tttttaatc aaggctgtg tatttttga 
      tgaaatcaa aaaataccg acgatcttt ttatttata 
      attatttaa agagagtgt aatgataat gaatttacc 
      ataaatgta ctaatatag ttatttatt tatttggtg 
      cggcatgat tttaagatg caacttttt tctcgattt 
      ggattatta aagttacgt cttttaatg atgtttccg 
      aacgtggta ggtgaatag gtttgtatt tagtgatgt 
      acaaatgaa attgttatt atttttgca ataaaacaa 
      atttaacct a
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