| General information | |||
| TC005852 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 6439502..6457074 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005852 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00944 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
- >TC005852 RA
MWFDLGRSE GFPTQYPPN QTPVTVDVL EAGFIAAFI ILLLSLLVL LPTSSLKKT LCLFVEITT LLTIGLLLL LGNFGQEWE VGHILTRTP YKAGSSAEI NASIGLKIG LRSVNVTLK SDQNPPNLA REIINYNER FSWTWDQDR LGFGPFAGH LQQEFRAAQ YRGLPTPIL WVVEYFVID GEGFRFGRF YRTAGWYSH VALWCAFAC WLVALLLFR SVILYGGYC LAACGALQL TANLIWLVV RNPIPLVVP FEDGTITTR FGLSYWITF ASGILCLVI AIIITLLDT KFHTLLYTF FGVDPLNSY DEVAYLTKN ELLLRTKNI SNDVPLIPM SNSPKEEED DSAEYVPVY IKRKTRAFG PPVVRSNKP KHLLEM
- >TC005852 RA
atgtggttc gatttggga cgaagtgag ggatttcca acccaatat ccccctaat caaaccccc gttactgtc gacgtgctg gaagccggc tttattgcc gctttcatc attttgttg ctttctctt ctcgttctt ttaccaaca tcgagttta aaaaagacc ttgtgcctg ttcgtcgaa ataacaacc ctcctaaca attggcctc ctcctcctc ctcggaaac ttcggccaa gaatgggaa gttggccac atcctcact agaacccct tacaaagcc ggctcttcg gccgaaatc aacgcctca attggccta aaaatcggc cttcggagc gtcaacgta acactcaaa tccgaccaa aaccctcca aacctcgcc agagaaata atcaattac aatgagaga ttttcctgg acttgggac caagaccgg cttggtttt ggccctttt gcaggccac ctccagcag gagtttcgc gcggcgcag tacaggggg ctcccaacg cccattttg tgggttgtg gagtatttt gtgatagat ggggaaggg ttccgtttt gggaggttt tacaggaca gcggggtgg tactcccat gtggcgcta tggtgtgcc tttgcttgt tggttggtg gctttgttg ttgttccgg tcggtgatc ttgtatggg ggctattgt ttggcggcg tgtggggct ctacaactc acggcgaac ttgatctgg ttggttgtg cgaaacccg attccgttg gtggttccg ttcgaggat gggacgatt acgacgagg tttgggctc agttattgg atcactttt gcgtcaggg attttatgt ttggtgatt gccataatt atcacttta ctcgacaca aaattccac acacttctt tacactttc tttggcgtt gacccactc aacagttac gatgaagtt gcttacttg accaaaaac gaactacta ctaagaaca aaaaatata tcaaacgat gtgcccctc atacccatg agtaattcc cctaaagaa gaagaagac gacagcgcc gaatacgtc cccgtgtat atcaagcgc aaaactcgg gcctttggc ccccctgtt gtccgaagc aacaaacct aaacactta ctggaaatg taa
- >TC005852 RA
tctattttg cttggtttc agatgagaa atactcgcc ggagcagaa agtgtacgt ttactgccc gtactgtta tcgcaagtt cgtcaatcg ctataatct gaaggtgca catccgaga caagcacga ggacagccc cagcgactt agactgtag ggtttgtgg taaggtaat gcgaaatag gagctgtct acgcgtaca catgtacca ccatcgcaa gcagcaatt ggaggccac gtgcgccgt tccaatcag ttaacaatt ttttatttt tgctcattg tgcacagct ctaggttag ggatttttt tgttgtgtt atatttatt tcataggct gttacgttc caaattcgg gcgggcgca ggttggacg cgataacta cctcgactg aaactaaat tcgagtatt aaccagtct tttgtattg gacaacctg gctaaagta ctacagggt gagtctgtt tgaaatata ttttaactt tttcccaaa attgatttt tttcactat tctttataa taaatattg actgattgt tttttttct gaaattgtt cgtaaagta gtactcttc agtactcat atgtcattg tacaatttt aagatagtc tgtaaaatt taatgtttt tatgaaatg ttgtctctt ttaaataaa aaaatacgc catttttgt tggttcaca aaacgtgtc ttacaaagt aaataatta atagttgtt attattaca aaaatatgt acaaaacgt cgatcatat tttgttttt aaataatgt aagaaaaac aatcagcga tttaatgtc aataatgta attgaaact tatctatta ctaagaata gttaaaaaa atcacctgc acaaagaaa aaagtaagt ctttaatgt cagacgttg gttttcatt ttcgatata aaattaaat atactttga acagactga ccttgtaaa taaggtgaa caatattat gaaactgag cgctttttt cttaagcta tttacgaac aaattaaaa tacttttta gcttttgag tttgttaaa acaacttgt gtaaattgt gtaactttt ttttaagtt gatattgaa acattcatc ttttcctta gttttttaa ataattttg tgaccaatt gggcttgat taaatttca gctacgcta aagcgtttt agaaatgtg ataactgat aagtatgaa attttttta gccaatatt taattccaa attctggtt atcgaattt ttttttcta ttttattgt tttgttcag gattttttg gaaaccaga annnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnacc agaaacaat ttatttttt cggaaagtg aatagttta cgtaaaaaa acgtttggt tctatactt ttgttcact ttaacattc catttcgcc tgcatgtat aaaccaaat atgagtttt tgtttattt atgagttgt ggtaccatt taatgctgc tctttgttt ttttttctt ctttaatta ttaacggtt ttgttgtta tgtttttgg atataagca tttacttgt gataaatta attttaata gcacaatca cattgggtt ctgaacatt tgtatgtgt gtgtatgtc tgacattcc atgtatgtt gttaagaaa cgggaccat tttctaata ttctaacca atattaata aacaaaaaa gttgaccta gttatttac ccgttcttt attccatat ttcccgtga tatccagta ctttagcat ttaatgaaa cttatttta ttgcaataa aacaaacat ttgaatgtc aactgttcg tatcttatc tcgccaaaa aggaagttt gggtttttc gtcaaaggg agtgtctga atcaacaac acaccagta tgtattaaa atgtggttc gatttggga cgaagtgag ggatttcca acccaatat ccccctaat caaaccccc gttactgtc gacgtgctg gaagccggc tttattgcc gctttcatc attttgttg ctttctctt ctcgttctt ttaccaaca tcgagttta aaaaagacc ttgtgcctg ttcgtcgaa ataacaacc ctcctaaca attggcctc ctcctcctc ctcggaaac ttcggccaa gaatgggaa gttggccac atcctcact agaacccct tacaaagcc ggctcttcg gccgaaatc aacgcctca attggccta aaaatcggc cttcggagc gtcaacgta acactcaaa tccgaccaa aaccctcca aacctcgcc agagaaata atcaattac aatgagaga ttttcctgg acttgggac caagaccgg cttggtttt ggccctttt gcaggccac ctccagcag gagtttcgc gcggcgcag tacaggggg ctcccaacg cccattttg tgggttgtg gagtatttt gtgatagat ggggaaggg ttccgtttt gggaggttt tacaggaca gcggggtgg tactcccat gtggcgcta tggtgtgcc tttgcttgt tggttggtg gctttgttg ttgttccgg tcggtgatc ttgtatggg ggctattgt ttggcggcg tgtggggct ctacaactc acggcgaac ttgatctgg ttggttgtg cgaaacccg attccgttg gtggttccg ttcgaggat gggacgatt acgacgagg tttgggctc agttattgg atcactttt gcgtcaggg attttatgt ttggtgatt gccataatt atcacttta ctcgacaca aaattccac acacttctt tacactttc tttggcgtt gacccactc aacagttac gatgaagtt gcttacttg accaaaaac gaactacta ctaagaaca aaaaatata tcaaacgat gtgcccctc atacccatg agtaattcc cctaaagaa gaagaagac gacagcgcc gaatacgtc cccgtgtat atcaagcgc aaaactcgg gcctttggc ccccctgtt gtccgaagc aacaaacct aaacactta ctggaaatg taaacaatg ttcaaataa taatacaat aaggtt
