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Gene information
General information
TC005852 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid53: 541286..558858
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005852
    iBeetle screen iB_00944
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005852 RA
      MWFDLGRSE GFPTQYPPN QTPVTVDVL EAGFIAAFI 
      ILLLSLLVL LPTSSLKKT LCLFVEITT LLTIGLLLL 
      LGNFGQEWE VGHILTRTP YKAGSSAEI NASIGLKIG 
      LRSVNVTLK SDQNPPNLA REIINYNER FSWTWDQDR 
      LGFGPFAGH LQQEFRAAQ YRGLPTPIL WVVEYFVID 
      GEGFRFGRF YRTAGWYSH VALWCAFAC WLVALLLFR 
      SVILYGGYC LAACGALQL TANLIWLVV RNPIPLVVP 
      FEDGTITTR FGLSYWITF ASGILCLVI AIIITLLDT 
      KFHTLLYTF FGVDPLNSY DEVAYLTKN ELLLRTKNI 
      SNDVPLIPM SNSPKEEED DSAEYVPVY IKRKTRAFG 
      PPVVRSNKP KHLLEM
    CDS sequence
    • >TC005852 RA
      atgtggttc gatttggga cgaagtgag ggatttcca 
      acccaatat ccccctaat caaaccccc gttactgtc 
      gacgtgctg gaagccggc tttattgcc gctttcatc 
      attttgttg ctttctctt ctcgttctt ttaccaaca 
      tcgagttta aaaaagacc ttgtgcctg ttcgtcgaa 
      ataacaacc ctcctaaca attggcctc ctcctcctc 
      ctcggaaac ttcggccaa gaatgggaa gttggccac 
      atcctcact agaacccct tacaaagcc ggctcttcg 
      gccgaaatc aacgcctca attggccta aaaatcggc 
      cttcggagc gtcaacgta acactcaaa tccgaccaa 
      aaccctcca aacctcgcc agagaaata atcaattac 
      aatgagaga ttttcctgg acttgggac caagaccgg 
      cttggtttt ggccctttt gcaggccac ctccagcag 
      gagtttcgc gcggcgcag tacaggggg ctcccaacg 
      cccattttg tgggttgtg gagtatttt gtgatagat 
      ggggaaggg ttccgtttt gggaggttt tacaggaca 
      gcggggtgg tactcccat gtggcgcta tggtgtgcc 
      tttgcttgt tggttggtg gctttgttg ttgttccgg 
      tcggtgatc ttgtatggg ggctattgt ttggcggcg 
      tgtggggct ctacaactc acggcgaac ttgatctgg 
      ttggttgtg cgaaacccg attccgttg gtggttccg 
      ttcgaggat gggacgatt acgacgagg tttgggctc 
      agttattgg atcactttt gcgtcaggg attttatgt 
      ttggtgatt gccataatt atcacttta ctcgacaca 
      aaattccac acacttctt tacactttc tttggcgtt 
      gacccactc aacagttac gatgaagtt gcttacttg 
      accaaaaac gaactacta ctaagaaca aaaaatata 
      tcaaacgat gtgcccctc atacccatg agtaattcc 
      cctaaagaa gaagaagac gacagcgcc gaatacgtc 
      cccgtgtat atcaagcgc aaaactcgg gcctttggc 
      ccccctgtt gtccgaagc aacaaacct aaacactta 
      ctggaaatg taa
    mRNA sequence
    • >TC005852 RA
      tctattttg cttggtttc agatgagaa atactcgcc 
      ggagcagaa agtgtacgt ttactgccc gtactgtta 
      tcgcaagtt cgtcaatcg ctataatct gaaggtgca 
      catccgaga caagcacga ggacagccc cagcgactt 
      agactgtag ggtttgtgg taaggtaat gcgaaatag 
      gagctgtct acgcgtaca catgtacca ccatcgcaa 
      gcagcaatt ggaggccac gtgcgccgt tccaatcag 
      ttaacaatt ttttatttt tgctcattg tgcacagct 
      ctaggttag ggatttttt tgttgtgtt atatttatt 
      tcataggct gttacgttc caaattcgg gcgggcgca 
      ggttggacg cgataacta cctcgactg aaactaaat 
      tcgagtatt aaccagtct tttgtattg gacaacctg 
      gctaaagta ctacagggt gagtctgtt tgaaatata 
      ttttaactt tttcccaaa attgatttt tttcactat 
      tctttataa taaatattg actgattgt tttttttct 
      gaaattgtt cgtaaagta gtactcttc agtactcat 
      atgtcattg tacaatttt aagatagtc tgtaaaatt 
      taatgtttt tatgaaatg ttgtctctt ttaaataaa 
      aaaatacgc catttttgt tggttcaca aaacgtgtc 
      ttacaaagt aaataatta atagttgtt attattaca 
      aaaatatgt acaaaacgt cgatcatat tttgttttt 
      aaataatgt aagaaaaac aatcagcga tttaatgtc 
      aataatgta attgaaact tatctatta ctaagaata 
      gttaaaaaa atcacctgc acaaagaaa aaagtaagt 
      ctttaatgt cagacgttg gttttcatt ttcgatata 
      aaattaaat atactttga acagactga ccttgtaaa 
      taaggtgaa caatattat gaaactgag cgctttttt 
      cttaagcta tttacgaac aaattaaaa tacttttta 
      gcttttgag tttgttaaa acaacttgt gtaaattgt 
      gtaactttt ttttaagtt gatattgaa acattcatc 
      ttttcctta gttttttaa ataattttg tgaccaatt 
      gggcttgat taaatttca gctacgcta aagcgtttt 
      agaaatgtg ataactgat aagtatgaa attttttta 
      gccaatatt taattccaa attctggtt atcgaattt 
      ttttttcta ttttattgt tttgttcag gattttttg 
      gaaaccaga annnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnacc agaaacaat 
      ttatttttt cggaaagtg aatagttta cgtaaaaaa 
      acgtttggt tctatactt ttgttcact ttaacattc 
      catttcgcc tgcatgtat aaaccaaat atgagtttt 
      tgtttattt atgagttgt ggtaccatt taatgctgc 
      tctttgttt ttttttctt ctttaatta ttaacggtt 
      ttgttgtta tgtttttgg atataagca tttacttgt 
      gataaatta attttaata gcacaatca cattgggtt 
      ctgaacatt tgtatgtgt gtgtatgtc tgacattcc 
      atgtatgtt gttaagaaa cgggaccat tttctaata 
      ttctaacca atattaata aacaaaaaa gttgaccta 
      gttatttac ccgttcttt attccatat ttcccgtga 
      tatccagta ctttagcat ttaatgaaa cttatttta 
      ttgcaataa aacaaacat ttgaatgtc aactgttcg 
      tatcttatc tcgccaaaa aggaagttt gggtttttc 
      gtcaaaggg agtgtctga atcaacaac acaccagta 
      tgtattaaa atgtggttc gatttggga cgaagtgag 
      ggatttcca acccaatat ccccctaat caaaccccc 
      gttactgtc gacgtgctg gaagccggc tttattgcc 
      gctttcatc attttgttg ctttctctt ctcgttctt 
      ttaccaaca tcgagttta aaaaagacc ttgtgcctg 
      ttcgtcgaa ataacaacc ctcctaaca attggcctc 
      ctcctcctc ctcggaaac ttcggccaa gaatgggaa 
      gttggccac atcctcact agaacccct tacaaagcc 
      ggctcttcg gccgaaatc aacgcctca attggccta 
      aaaatcggc cttcggagc gtcaacgta acactcaaa 
      tccgaccaa aaccctcca aacctcgcc agagaaata 
      atcaattac aatgagaga ttttcctgg acttgggac 
      caagaccgg cttggtttt ggccctttt gcaggccac 
      ctccagcag gagtttcgc gcggcgcag tacaggggg 
      ctcccaacg cccattttg tgggttgtg gagtatttt 
      gtgatagat ggggaaggg ttccgtttt gggaggttt 
      tacaggaca gcggggtgg tactcccat gtggcgcta 
      tggtgtgcc tttgcttgt tggttggtg gctttgttg 
      ttgttccgg tcggtgatc ttgtatggg ggctattgt 
      ttggcggcg tgtggggct ctacaactc acggcgaac 
      ttgatctgg ttggttgtg cgaaacccg attccgttg 
      gtggttccg ttcgaggat gggacgatt acgacgagg 
      tttgggctc agttattgg atcactttt gcgtcaggg 
      attttatgt ttggtgatt gccataatt atcacttta 
      ctcgacaca aaattccac acacttctt tacactttc 
      tttggcgtt gacccactc aacagttac gatgaagtt 
      gcttacttg accaaaaac gaactacta ctaagaaca 
      aaaaatata tcaaacgat gtgcccctc atacccatg 
      agtaattcc cctaaagaa gaagaagac gacagcgcc 
      gaatacgtc cccgtgtat atcaagcgc aaaactcgg 
      gcctttggc ccccctgtt gtccgaagc aacaaacct 
      aaacactta ctggaaatg taaacaatg ttcaaataa 
      taatacaat aaggtt