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Gene information
General information
TC005837 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6973708..6975442
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005837
    iBeetle screen iB_00941

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005837 RA
      MSSSEDELF DIDWGASNV FDCFDYVDP YVLSPQIQA 
      VLENDIVGL RRLLQEGKS VNINDNKGN TPLHHAVQS 
      QGNLTSIFN CLIELEDIR LDAANFNGE TPLMLALKN 
      AYYVRKLLQ KGADPNLRY PNGDSLLHK ATDVGVVRL 
      LLEHGADIN AQNFNGETP LFHACQQKE IELVHLYLY 
      HGADVSISD NCGLLAFDI CPESVDSLF YLTFDEHTE 
      GTEVTSEVL DVVYHEFGF CEFYRTLLN IAKIRNHCC 
      KFDSYAGRN FMAFFTYDL NLSIDMVER FPKENLEAQ 
      CFAKLLKFF NVIKYKDKF PHLFEMFSR KLGVSAVPS 
      LVELSRNAA RKFIISKLK VEDNVQFLT VVNHLPVPN 
      VVKQIIIYQ KEIYNI
    • >TC005837 RB
      MSSSEDELF DIDWGASNV FDCFDYVDP YVLSPQIQA 
      VLENDIVGL RRLLQEGKS VNINDNKGN TPLHHAVQS 
      QGNLTSIFN CLIELEDIR LDAANFNGE TPLMLALKN 
      AYYVRKLLQ KGADPNLRY PNGDSLLHK ATDVGVVRL 
      LLEHGADIN AQNFNGETP LFHACQQKE IELVHLYLY 
      HGADVSISD NCGLLAFDI CPESVDSLF YLTFDEHTE 
      LTRNVTYTR KQLNEFVAQ ISLPWCEVF FNLVDSFIN 
      REFANSMQV FRLMSARTH TCDVVTIED FLYIFLYFY 
      VRDNSLCPL LRNIHLRGQ LLIQFAMKF VCYCASQGT 
      EVTSEVLDV VYHEFGFCE FYRTLLNIA KIRNHCCKF 
      DSYAGRNFM AFFTYDLNL SIDMVERFP KENLEAQCF 
      AKLLKFFNV IKYKDKFPH LFEMFSRKL GVSAVPSLV 
      ELSRNAARK FIISKLKVE DNVQFLTVV NHLPVPNVV 
      KQIIIYQKE IYNI
    CDS sequence
    • >TC005837 RA
      atgtccagc agcgaagac gaactcttc gatatcgac 
      tggggggct tccaatgta ttcgactgc ttcgattac 
      gtggacccg tatgtttta tcgccccaa attcaggca 
      gttttggag aacgacatc gttggactt cgccgcctc 
      ttgcaagaa ggaaaatca gtcaacatc aacgacaac 
      aaaggcaac actcctctt caccacgct gtacaatca 
      cagggtaat ttaacatcc atttttaat tgtttgatc 
      gaactggag gacattcga ctcgacgcc gccaatttc 
      aacggggaa accccccta atgctcgcc ctcaaaaac 
      gcgtattac gttcgcaaa ttgctgcaa aagggggcc 
      gaccccaac ttgcgctac cccaacggg gactcttta 
      ttgcacaaa gccacagat gtgggggtc gtgcggttg 
      ttgttggaa catggggct gatattaac gcgcaaaat 
      ttcaacggc gagactcct ctatttcat gcttgccaa 
      caaaaggaa atagaattg gtacacttg tatttatac 
      cacggggct gatgtgtcg attagtgac aattgcggt 
      ttattggca tttgatatt tgtcccgaa agtgtggac 
      agtcttttt tacttaact tttgacgaa cacactgag 
      gggactgaa gtcacttca gaggttttg gacgtggtt 
      taccacgag tttggtttt tgcgaattt taccgaacg 
      ttactgaat attgccaaa attcgaaat cattgttgc 
      aagtttgat tcgtatgcg ggtcgcaat tttatggcg 
      tttttcacc tacgatttg aatttaagc attgatatg 
      gtcgagcgt ttcccaaaa gaaaatctt gaagcgcag 
      tgttttgca aaattgttg aaattcttt aatgttata 
      aagtacaag gataagttt ccgcatttg tttgagatg 
      tttagtcgt aaacttgga gtatcagca gtgccttct 
      ttggtcgag ttgagtcga aatgccgct cgcaaattt 
      atcatttcc aagctcaaa gtggaggat aatgtccag 
      tttttgact gttgttaac caccttccc gttcctaac 
      gttgttaaa caaataatt atttaccaa aaagaaatt 
      tacaatatt taa
    • >TC005837 RB
      atgtccagc agcgaagac gaactcttc gatatcgac 
      tggggggct tccaatgta ttcgactgc ttcgattac 
      gtggacccg tatgtttta tcgccccaa attcaggca 
      gttttggag aacgacatc gttggactt cgccgcctc 
      ttgcaagaa ggaaaatca gtcaacatc aacgacaac 
      aaaggcaac actcctctt caccacgct gtacaatca 
      cagggtaat ttaacatcc atttttaat tgtttgatc 
      gaactggag gacattcga ctcgacgcc gccaatttc 
      aacggggaa accccccta atgctcgcc ctcaaaaac 
      gcgtattac gttcgcaaa ttgctgcaa aagggggcc 
      gaccccaac ttgcgctac cccaacggg gactcttta 
      ttgcacaaa gccacagat gtgggggtc gtgcggttg 
      ttgttggaa catggggct gatattaac gcgcaaaat 
      ttcaacggc gagactcct ctatttcat gcttgccaa 
      caaaaggaa atagaattg gtacacttg tatttatac 
      cacggggct gatgtgtcg attagtgac aattgcggt 
      ttattggca tttgatatt tgtcccgaa agtgtggac 
      agtcttttt tacttaact tttgacgaa cacactgag 
      ctaacaaga aacgttacc tacactaga aaacaactg 
      aatgagttc gtggctcaa atcagccta ccttggtgt 
      gaggttttt ttcaattta gttgattcg tttattaac 
      cgagaattt gcaaattca atgcaagtc tttcgttta 
      atgagtgct agaacacat acttgtgac gtggtcaca 
      attgaagat tttttgtac attttttta tatttttat 
      gttcgggac aattcgctg tgtccgctt ttgcgaaat 
      atccatctg agaggccag ttgttaata cagtttgcc 
      atgaaattc gtgtgttat tgtgcaagt caggggact 
      gaagtcact tcagaggtt ttggacgtg gtttaccac 
      gagtttggt ttttgcgaa ttttaccga acgttactg 
      aatattgcc aaaattcga aatcattgt tgcaagttt 
      gattcgtat gcgggtcgc aattttatg gcgtttttc 
      acctacgat ttgaattta agcattgat atggtcgag 
      cgtttccca aaagaaaat cttgaagcg cagtgtttt 
      gcaaaattg ttgaaattc tttaatgtt ataaagtac 
      aaggataag tttccgcat ttgtttgag atgtttagt 
      cgtaaactt ggagtatca gcagtgcct tctttggtc 
      gagttgagt cgaaatgcc gctcgcaaa tttatcatt 
      tccaagctc aaagtggag gataatgtc cagtttttg 
      actgttgtt aaccacctt cccgttcct aacgttgtt 
      aaacaaata attatttac caaaaagaa atttacaat 
      atttaa
    mRNA sequence
    • >TC005837 RA
      cgctttcgc taaaaacgg gagataaac agagagata 
      cacttttcc ctgtaaaca attaaagtg aggttatga 
      ttagtgccc agaaaaact ttttaatgt ttgattgta 
      ataattgtt tttctcaag cttgggata accacaatg 
      tccagcagc gaagacgaa ctcttcgat atcgactgg 
      ggggcttcc aatgtattc gactgcttc gattacgtg 
      gacccgtat gttttatcg ccccaaatt caggcagtt 
      ttggagaac gacatcgtt ggacttcgc cgcctcttg 
      caagaagga aaatcagtc aacatcaac gacaacaaa 
      ggcaacact cctcttcac cacgctgta caatcacag 
      ggtaattta acatccatt tttaattgt ttgatcgaa 
      ctggaggac attcgactc gacgccgcc aatttcaac 
      ggggaaacc cccctaatg ctcgccctc aaaaacgcg 
      tattacgtt cgcaaattg ctgcaaaag ggggccgac 
      cccaacttg cgctacccc aacggggac tctttattg 
      cacaaagcc acagatgtg ggggtcgtg cggttgttg 
      ttggaacat ggggctgat attaacgcg caaaatttc 
      aacggcgag actcctcta tttcatgct tgccaacaa 
      aaggaaata gaattggta cacttgtat ttataccac 
      ggggctgat gtgtcgatt agtgacaat tgcggttta 
      ttggcattt gatatttgt cccgaaagt gtggacagt 
      cttttttac ttaactttt gacgaacac actgagggg 
      actgaagtc acttcagag gttttggac gtggtttac 
      cacgagttt ggtttttgc gaattttac cgaacgtta 
      ctgaatatt gccaaaatt cgaaatcat tgttgcaag 
      tttgattcg tatgcgggt cgcaatttt atggcgttt 
      ttcacctac gatttgaat ttaagcatt gatatggtc 
      gagcgtttc ccaaaagaa aatcttgaa gcgcagtgt 
      tttgcaaaa ttgttgaaa ttctttaat gttataaag 
      tacaaggat aagtttccg catttgttt gagatgttt 
      agtcgtaaa cttggagta tcagcagtg ccttctttg 
      gtcgagttg agtcgaaat gccgctcgc aaatttatc 
      atttccaag ctcaaagtg gaggataat gtccagttt 
      ttgactgtt gttaaccac cttcccgtt cctaacgtt 
      gttaaacaa ataattatt taccaaaaa gaaatttac 
      aatatttaa tgttatgat gaaataaat ttttgtaat 
      tact
    • >TC005837 RB
      cgctttcgc taaaaacgg gagataaac agagagata 
      cacttttcc ctgtaaaca attaaagtg aggttatga 
      ttagtgccc agaaaaact ttttaatgt ttgattgta 
      ataattgtt tttctcaag cttgggata accacaatg 
      tccagcagc gaagacgaa ctcttcgat atcgactgg 
      ggggcttcc aatgtattc gactgcttc gattacgtg 
      gacccgtat gttttatcg ccccaaatt caggcagtt 
      ttggagaac gacatcgtt ggacttcgc cgcctcttg 
      caagaagga aaatcagtc aacatcaac gacaacaaa 
      ggcaacact cctcttcac cacgctgta caatcacag 
      ggtaattta acatccatt tttaattgt ttgatcgaa 
      ctggaggac attcgactc gacgccgcc aatttcaac 
      ggggaaacc cccctaatg ctcgccctc aaaaacgcg 
      tattacgtt cgcaaattg ctgcaaaag ggggccgac 
      cccaacttg cgctacccc aacggggac tctttattg 
      cacaaagcc acagatgtg ggggtcgtg cggttgttg 
      ttggaacat ggggctgat attaacgcg caaaatttc 
      aacggcgag actcctcta tttcatgct tgccaacaa 
      aaggaaata gaattggta cacttgtat ttataccac 
      ggggctgat gtgtcgatt agtgacaat tgcggttta 
      ttggcattt gatatttgt cccgaaagt gtggacagt 
      cttttttac ttaactttt gacgaacac actgagcta 
      acaagaaac gttacctac actagaaaa caactgaat 
      gagttcgtg gctcaaatc agcctacct tggtgtgag 
      gtttttttc aatttagtt gattcgttt attaaccga 
      gaatttgca aattcaatg caagtcttt cgtttaatg 
      agtgctaga acacatact tgtgacgtg gtcacaatt 
      gaagatttt ttgtacatt tttttatat ttttatgtt 
      cgggacaat tcgctgtgt ccgcttttg cgaaatatc 
      catctgaga ggccagttg ttaatacag tttgccatg 
      aaattcgtg tgttattgt gcaagtcag gggactgaa 
      gtcacttca gaggttttg gacgtggtt taccacgag 
      tttggtttt tgcgaattt taccgaacg ttactgaat 
      attgccaaa attcgaaat cattgttgc aagtttgat 
      tcgtatgcg ggtcgcaat tttatggcg tttttcacc 
      tacgatttg aatttaagc attgatatg gtcgagcgt 
      ttcccaaaa gaaaatctt gaagcgcag tgttttgca 
      aaattgttg aaattcttt aatgttata aagtacaag 
      gataagttt ccgcatttg tttgagatg tttagtcgt 
      aaacttgga gtatcagca gtgccttct ttggtcgag 
      ttgagtcga aatgccgct cgcaaattt atcatttcc 
      aagctcaaa gtggaggat aatgtccag tttttgact 
      gttgttaac caccttccc gttcctaac gttgttaaa 
      caaataatt atttaccaa aaagaaatt tacaatatt 
      taatgttat gatgaaata aatttttgt aattact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY