| General information | |||
| TC005837 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 6973708..6975442 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005837 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00941 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - (41 dpi)
- >TC005837 RA
MSSSEDELF DIDWGASNV FDCFDYVDP YVLSPQIQA VLENDIVGL RRLLQEGKS VNINDNKGN TPLHHAVQS QGNLTSIFN CLIELEDIR LDAANFNGE TPLMLALKN AYYVRKLLQ KGADPNLRY PNGDSLLHK ATDVGVVRL LLEHGADIN AQNFNGETP LFHACQQKE IELVHLYLY HGADVSISD NCGLLAFDI CPESVDSLF YLTFDEHTE GTEVTSEVL DVVYHEFGF CEFYRTLLN IAKIRNHCC KFDSYAGRN FMAFFTYDL NLSIDMVER FPKENLEAQ CFAKLLKFF NVIKYKDKF PHLFEMFSR KLGVSAVPS LVELSRNAA RKFIISKLK VEDNVQFLT VVNHLPVPN VVKQIIIYQ KEIYNI
- >TC005837 RB
MSSSEDELF DIDWGASNV FDCFDYVDP YVLSPQIQA VLENDIVGL RRLLQEGKS VNINDNKGN TPLHHAVQS QGNLTSIFN CLIELEDIR LDAANFNGE TPLMLALKN AYYVRKLLQ KGADPNLRY PNGDSLLHK ATDVGVVRL LLEHGADIN AQNFNGETP LFHACQQKE IELVHLYLY HGADVSISD NCGLLAFDI CPESVDSLF YLTFDEHTE LTRNVTYTR KQLNEFVAQ ISLPWCEVF FNLVDSFIN REFANSMQV FRLMSARTH TCDVVTIED FLYIFLYFY VRDNSLCPL LRNIHLRGQ LLIQFAMKF VCYCASQGT EVTSEVLDV VYHEFGFCE FYRTLLNIA KIRNHCCKF DSYAGRNFM AFFTYDLNL SIDMVERFP KENLEAQCF AKLLKFFNV IKYKDKFPH LFEMFSRKL GVSAVPSLV ELSRNAARK FIISKLKVE DNVQFLTVV NHLPVPNVV KQIIIYQKE IYNI
- >TC005837 RA
atgtccagc agcgaagac gaactcttc gatatcgac tggggggct tccaatgta ttcgactgc ttcgattac gtggacccg tatgtttta tcgccccaa attcaggca gttttggag aacgacatc gttggactt cgccgcctc ttgcaagaa ggaaaatca gtcaacatc aacgacaac aaaggcaac actcctctt caccacgct gtacaatca cagggtaat ttaacatcc atttttaat tgtttgatc gaactggag gacattcga ctcgacgcc gccaatttc aacggggaa accccccta atgctcgcc ctcaaaaac gcgtattac gttcgcaaa ttgctgcaa aagggggcc gaccccaac ttgcgctac cccaacggg gactcttta ttgcacaaa gccacagat gtgggggtc gtgcggttg ttgttggaa catggggct gatattaac gcgcaaaat ttcaacggc gagactcct ctatttcat gcttgccaa caaaaggaa atagaattg gtacacttg tatttatac cacggggct gatgtgtcg attagtgac aattgcggt ttattggca tttgatatt tgtcccgaa agtgtggac agtcttttt tacttaact tttgacgaa cacactgag gggactgaa gtcacttca gaggttttg gacgtggtt taccacgag tttggtttt tgcgaattt taccgaacg ttactgaat attgccaaa attcgaaat cattgttgc aagtttgat tcgtatgcg ggtcgcaat tttatggcg tttttcacc tacgatttg aatttaagc attgatatg gtcgagcgt ttcccaaaa gaaaatctt gaagcgcag tgttttgca aaattgttg aaattcttt aatgttata aagtacaag gataagttt ccgcatttg tttgagatg tttagtcgt aaacttgga gtatcagca gtgccttct ttggtcgag ttgagtcga aatgccgct cgcaaattt atcatttcc aagctcaaa gtggaggat aatgtccag tttttgact gttgttaac caccttccc gttcctaac gttgttaaa caaataatt atttaccaa aaagaaatt tacaatatt taa
- >TC005837 RB
atgtccagc agcgaagac gaactcttc gatatcgac tggggggct tccaatgta ttcgactgc ttcgattac gtggacccg tatgtttta tcgccccaa attcaggca gttttggag aacgacatc gttggactt cgccgcctc ttgcaagaa ggaaaatca gtcaacatc aacgacaac aaaggcaac actcctctt caccacgct gtacaatca cagggtaat ttaacatcc atttttaat tgtttgatc gaactggag gacattcga ctcgacgcc gccaatttc aacggggaa accccccta atgctcgcc ctcaaaaac gcgtattac gttcgcaaa ttgctgcaa aagggggcc gaccccaac ttgcgctac cccaacggg gactcttta ttgcacaaa gccacagat gtgggggtc gtgcggttg ttgttggaa catggggct gatattaac gcgcaaaat ttcaacggc gagactcct ctatttcat gcttgccaa caaaaggaa atagaattg gtacacttg tatttatac cacggggct gatgtgtcg attagtgac aattgcggt ttattggca tttgatatt tgtcccgaa agtgtggac agtcttttt tacttaact tttgacgaa cacactgag ctaacaaga aacgttacc tacactaga aaacaactg aatgagttc gtggctcaa atcagccta ccttggtgt gaggttttt ttcaattta gttgattcg tttattaac cgagaattt gcaaattca atgcaagtc tttcgttta atgagtgct agaacacat acttgtgac gtggtcaca attgaagat tttttgtac attttttta tatttttat gttcgggac aattcgctg tgtccgctt ttgcgaaat atccatctg agaggccag ttgttaata cagtttgcc atgaaattc gtgtgttat tgtgcaagt caggggact gaagtcact tcagaggtt ttggacgtg gtttaccac gagtttggt ttttgcgaa ttttaccga acgttactg aatattgcc aaaattcga aatcattgt tgcaagttt gattcgtat gcgggtcgc aattttatg gcgtttttc acctacgat ttgaattta agcattgat atggtcgag cgtttccca aaagaaaat cttgaagcg cagtgtttt gcaaaattg ttgaaattc tttaatgtt ataaagtac aaggataag tttccgcat ttgtttgag atgtttagt cgtaaactt ggagtatca gcagtgcct tctttggtc gagttgagt cgaaatgcc gctcgcaaa tttatcatt tccaagctc aaagtggag gataatgtc cagtttttg actgttgtt aaccacctt cccgttcct aacgttgtt aaacaaata attatttac caaaaagaa atttacaat atttaa
- >TC005837 RA
cgctttcgc taaaaacgg gagataaac agagagata cacttttcc ctgtaaaca attaaagtg aggttatga ttagtgccc agaaaaact ttttaatgt ttgattgta ataattgtt tttctcaag cttgggata accacaatg tccagcagc gaagacgaa ctcttcgat atcgactgg ggggcttcc aatgtattc gactgcttc gattacgtg gacccgtat gttttatcg ccccaaatt caggcagtt ttggagaac gacatcgtt ggacttcgc cgcctcttg caagaagga aaatcagtc aacatcaac gacaacaaa ggcaacact cctcttcac cacgctgta caatcacag ggtaattta acatccatt tttaattgt ttgatcgaa ctggaggac attcgactc gacgccgcc aatttcaac ggggaaacc cccctaatg ctcgccctc aaaaacgcg tattacgtt cgcaaattg ctgcaaaag ggggccgac cccaacttg cgctacccc aacggggac tctttattg cacaaagcc acagatgtg ggggtcgtg cggttgttg ttggaacat ggggctgat attaacgcg caaaatttc aacggcgag actcctcta tttcatgct tgccaacaa aaggaaata gaattggta cacttgtat ttataccac ggggctgat gtgtcgatt agtgacaat tgcggttta ttggcattt gatatttgt cccgaaagt gtggacagt cttttttac ttaactttt gacgaacac actgagggg actgaagtc acttcagag gttttggac gtggtttac cacgagttt ggtttttgc gaattttac cgaacgtta ctgaatatt gccaaaatt cgaaatcat tgttgcaag tttgattcg tatgcgggt cgcaatttt atggcgttt ttcacctac gatttgaat ttaagcatt gatatggtc gagcgtttc ccaaaagaa aatcttgaa gcgcagtgt tttgcaaaa ttgttgaaa ttctttaat gttataaag tacaaggat aagtttccg catttgttt gagatgttt agtcgtaaa cttggagta tcagcagtg ccttctttg gtcgagttg agtcgaaat gccgctcgc aaatttatc atttccaag ctcaaagtg gaggataat gtccagttt ttgactgtt gttaaccac cttcccgtt cctaacgtt gttaaacaa ataattatt taccaaaaa gaaatttac aatatttaa tgttatgat gaaataaat ttttgtaat tact
- >TC005837 RB
cgctttcgc taaaaacgg gagataaac agagagata cacttttcc ctgtaaaca attaaagtg aggttatga ttagtgccc agaaaaact ttttaatgt ttgattgta ataattgtt tttctcaag cttgggata accacaatg tccagcagc gaagacgaa ctcttcgat atcgactgg ggggcttcc aatgtattc gactgcttc gattacgtg gacccgtat gttttatcg ccccaaatt caggcagtt ttggagaac gacatcgtt ggacttcgc cgcctcttg caagaagga aaatcagtc aacatcaac gacaacaaa ggcaacact cctcttcac cacgctgta caatcacag ggtaattta acatccatt tttaattgt ttgatcgaa ctggaggac attcgactc gacgccgcc aatttcaac ggggaaacc cccctaatg ctcgccctc aaaaacgcg tattacgtt cgcaaattg ctgcaaaag ggggccgac cccaacttg cgctacccc aacggggac tctttattg cacaaagcc acagatgtg ggggtcgtg cggttgttg ttggaacat ggggctgat attaacgcg caaaatttc aacggcgag actcctcta tttcatgct tgccaacaa aaggaaata gaattggta cacttgtat ttataccac ggggctgat gtgtcgatt agtgacaat tgcggttta ttggcattt gatatttgt cccgaaagt gtggacagt cttttttac ttaactttt gacgaacac actgagcta acaagaaac gttacctac actagaaaa caactgaat gagttcgtg gctcaaatc agcctacct tggtgtgag gtttttttc aatttagtt gattcgttt attaaccga gaatttgca aattcaatg caagtcttt cgtttaatg agtgctaga acacatact tgtgacgtg gtcacaatt gaagatttt ttgtacatt tttttatat ttttatgtt cgggacaat tcgctgtgt ccgcttttg cgaaatatc catctgaga ggccagttg ttaatacag tttgccatg aaattcgtg tgttattgt gcaagtcag gggactgaa gtcacttca gaggttttg gacgtggtt taccacgag tttggtttt tgcgaattt taccgaacg ttactgaat attgccaaa attcgaaat cattgttgc aagtttgat tcgtatgcg ggtcgcaat tttatggcg tttttcacc tacgatttg aatttaagc attgatatg gtcgagcgt ttcccaaaa gaaaatctt gaagcgcag tgttttgca aaattgttg aaattcttt aatgttata aagtacaag gataagttt ccgcatttg tttgagatg tttagtcgt aaacttgga gtatcagca gtgccttct ttggtcgag ttgagtcga aatgccgct cgcaaattt atcatttcc aagctcaaa gtggaggat aatgtccag tttttgact gttgttaac caccttccc gttcctaac gttgttaaa caaataatt atttaccaa aaagaaatt tacaatatt taatgttat gatgaaata aatttttgt aattact
