| General information | |||
| TC005835 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 6979731..6987768 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005835 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00940 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
mature egg not present - Number of animals showing the phenotype: 4
| 2 eggs |
| Comment: 2 out of 2 |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005835 RA
MEDNTQLSF PEEDEDLEA LRLAALQSL KRKASNEAQ QMGHFGGIK DKGNHFNGS PRGKHKRGK FSRLQQGRI PRNGQFNGW SRNSNLIAV PMVTEDEKL PESTKNVTE DVPKLVLPQ DRYCKADKS PERSDPIST KFDRYDNSD ESDDESEEE RSKSETEEK PELTRSNSL EALMAELEN EIQGVTAEK SPPKPKKKR KVSVIEPEE KIGENAVKE NVKPEPEVK KSPNFRRKW QKPRRPFHP PSLVHPVAP PPEILPPPV VAFAPPPIP PFAPYMGFD GVYAPPIYE SPPPQLPPL HMAPPIYER PLSPIGVNT DFGNGIMAP LSPRSAAFV MENRAIIEK RKRRSYSRS PTPVRFRKR ASLSPLRRS LSPSPVRKV SPRLSPKKQ SSVRERLGA KNGKTEEVK KEEKEDPVL EARRKKFER NEITMTEGV IRLKKPESE EVKKEECVE DELEAEEID EDALLEGAD SIILDPKLD TLFSDEESD SDNEGRFKT KQETNQKVP ILPFTKLIN GAKSEVKTD LRLEEKKTD RRKRFRSRT PLKKPDVKL ASERKIEIK IRNPAKYEK SEPEEKVRK VEVADEKPE EKVESSVTE GDLRTQLSR KRAERQKHP LPDGVPSRL LQNALQGVG FRKVKKSKK DENTEGKLP IHLRLGAAT EPSGKKSRK QKKGSQEQV LCFVASFLI IIIFSLKLF VSTLVSKRF LIGALTTKR FALKNRVFI FVTHL
- >TC005835 RB
MEDNTQLSF PEEDEDLEA LRLAALQSL KRKASNEAQ QMGHFGGIK DKGNHFNGS PRGKHKRGK FSRLQQGRI PRNGQFNGW SRNSNLIAV PMVTEDEKL PESTKNVTE DVPKLVLPQ DRYCKADKS PERSDPIST KFDRYDNSD ESDDESEEE RSKSETEEK PELTRSNSL EALMAELEN EIQGVTAEK SPPKPKKKR KVSVIEPEE KIGENAVKE NVKPEPEVK KSPNFRRKW QKPRRPFHP PSLVHPVAP PPEILPPPV VAFAPPPIP PFAPYMGFD GVYAPPIYE SPPPQLPPL HMAPPIYER PLSPIGVNT DFGNGIMAP LSPRSAAFV MENRAIIEK RKRRSYSRS PTPVRFRKR ASLSPLRRS LSPSPVRKV SPRLSPKKQ SSVRERLGA KNGKTEEVK KEEKEDPVL EARRKKFER NEITMTEGV IRLKKPESE EVKKEECVE DELEAEEID EDALLEGAD SIILDPKLD TLFSDEESD SDNEGRFKT KQETNQKVP ILPFTKLIN GAKSEVKTD LRLEEKKTD RRKRFRSRT PLKKPDVKL ASERKIEIK IRNPAKYEK SEPEEKVRK VEVADEKPE EKVESSVTE GDLRTQLSR KRAERQKHP LPDGVPSRL LQNALQGVG FRKVKKSKK DENTEGKLP IHLRLGAAT EPSGKKSRK QKKGSQEQV
- >TC005835 RA
atggaagac aacactcag ttgtctttt ccagaagag gacgaagat ttggaagct ttacgatta gcagcctta cagtctttg aaacgtaaa gccagtaat gaagcccaa caaatgggt cactttggt ggtattaaa gacaagggg aatcatttt aatgggtca ccgcgaggc aagcacaag aggggcaaa ttttcaaga ctacagcaa ggtcgcatt cctagaaac ggccagttt aacggttgg tcacgaaat tcgaatttg atcgccgtt ccaatggtc acagaagac gagaaatta ccggaaagt acgaaaaat gtgactgag gatgtccca aaattggtg cttccccaa gacaggtat tgcaaagcg gacaaatcc cccgaacgt tctgacccc atttcaacc aaattcgac cgttatgat aactcagac gagtcagac gacgagtct gaggaggaa cgttccaag tccgaaacg gaggaaaag ccagagttg acccgttcg aacagcctt gaggccctg atggccgaa ctcgaaaat gaaatacaa ggagtgacg gcggaaaaa tcgccacca aaacccaag aaaaaacga aaagtttcg gtcatcgaa ccggaggag aaaatcggc gaaaacgcc gttaaagaa aatgtgaaa ccggaaccg gaggtgaaa aaatcaccg aactttcgg cgcaaatgg caaaagcct cgtcggccg ttccatccg ccgagttta gtgcatccc gtggcgccg ccaccggag atattgccg cctccggtg gtggcgttt gccccgcct ccaattccg ccgtttgcg ccttatatg ggttttgat ggtgtttac gccccgcct atttacgaa tctccgcct ccacagttg ccaccgttg catatggcg ccgcctatt tacgaacgc cctctttct cccattggt gttaatacc gattttggg aatgggatc atggcgccg ctgtcgcca cggagtgcc gcttttgtg atggaaaat cgggctatt attgagaag aggaaacgc aggtcgtac tcccggagt ccgactccg gttaggttc cggaaaagg gcgtcttta tcgcctttg aggcgttcc ctatctcca tcacctgtc cgaaaagta tcgccacgc ctatctccg aaaaaacaa tcatcggtt cgtgaacgg ttaggagcg aagaacggc aagactgaa gaagtgaag aaagaggaa aaagaagat ccggttttg gaagcgcga cgaaaaaaa tttgaacgt aacgaaatc acaatgact gaaggtgtg atacgtttg aagaaaccc gaaagtgag gaagtgaag aaggaggaa tgcgtcgaa gacgagctg gaggcggaa gaaatcgat gaagatgcg ttgttggaa ggcgccgat tcgattatt ttagaccca aaactcgat acacttttc tccgatgaa gaatcggac agtgacaac gaagggcgg tttaaaacc aagcaagaa acgaatcag aaagttcca attttgccg tttactaaa ctaatcaac ggcgcgaag agtgaagtg aagaccgat cttcggctg gaagagaag aagaccgat aggaggaaa cggtttcgg agtcggacg ccgttaaag aaaccggac gttaagctt gccagtgaa agaaaaatc gaaatcaaa attcgaaat ccggcgaaa tatgaaaaa agcgagccg gaggaaaag gtgaggaag gtggaagtg gcggatgag aaaccggaa gagaaggtt gagtcgagt gtgactgaa ggtgatttg aggacgcaa ctgagtcgg aaacgagcc gagagacaa aaacatcct cttccggac ggcgtgccg tcgagactt ttgcagaat gcactacaa ggagttggc ttcaggaaa gtgaaaaag agtaaaaaa gacgaaaat acagaaggg aaattgccg attcacttg cgactgggg gctgccacc gagccgagt gggaaaaag tcgaggaag caaaagaaa ggaagtcag gagcaggta ttgtgtttt gttgcgtca tttcttatt attattatt tttagttta aaattgttc gttagtacg ttagtatca aaacgattt ctaatcggt gcactaaca accaaacgt ttcgctttg aaaaaccgc gtttttatt ttcgtgact catctgtga
- >TC005835 RB
atggaagac aacactcag ttgtctttt ccagaagag gacgaagat ttggaagct ttacgatta gcagcctta cagtctttg aaacgtaaa gccagtaat gaagcccaa caaatgggt cactttggt ggtattaaa gacaagggg aatcatttt aatgggtca ccgcgaggc aagcacaag aggggcaaa ttttcaaga ctacagcaa ggtcgcatt cctagaaac ggccagttt aacggttgg tcacgaaat tcgaatttg atcgccgtt ccaatggtc acagaagac gagaaatta ccggaaagt acgaaaaat gtgactgag gatgtccca aaattggtg cttccccaa gacaggtat tgcaaagcg gacaaatcc cccgaacgt tctgacccc atttcaacc aaattcgac cgttatgat aactcagac gagtcagac gacgagtct gaggaggaa cgttccaag tccgaaacg gaggaaaag ccagagttg acccgttcg aacagcctt gaggccctg atggccgaa ctcgaaaat gaaatacaa ggagtgacg gcggaaaaa tcgccacca aaacccaag aaaaaacga aaagtttcg gtcatcgaa ccggaggag aaaatcggc gaaaacgcc gttaaagaa aatgtgaaa ccggaaccg gaggtgaaa aaatcaccg aactttcgg cgcaaatgg caaaagcct cgtcggccg ttccatccg ccgagttta gtgcatccc gtggcgccg ccaccggag atattgccg cctccggtg gtggcgttt gccccgcct ccaattccg ccgtttgcg ccttatatg ggttttgat ggtgtttac gccccgcct atttacgaa tctccgcct ccacagttg ccaccgttg catatggcg ccgcctatt tacgaacgc cctctttct cccattggt gttaatacc gattttggg aatgggatc atggcgccg ctgtcgcca cggagtgcc gcttttgtg atggaaaat cgggctatt attgagaag aggaaacgc aggtcgtac tcccggagt ccgactccg gttaggttc cggaaaagg gcgtcttta tcgcctttg aggcgttcc ctatctcca tcacctgtc cgaaaagta tcgccacgc ctatctccg aaaaaacaa tcatcggtt cgtgaacgg ttaggagcg aagaacggc aagactgaa gaagtgaag aaagaggaa aaagaagat ccggttttg gaagcgcga cgaaaaaaa tttgaacgt aacgaaatc acaatgact gaaggtgtg atacgtttg aagaaaccc gaaagtgag gaagtgaag aaggaggaa tgcgtcgaa gacgagctg gaggcggaa gaaatcgat gaagatgcg ttgttggaa ggcgccgat tcgattatt ttagaccca aaactcgat acacttttc tccgatgaa gaatcggac agtgacaac gaagggcgg tttaaaacc aagcaagaa acgaatcag aaagttcca attttgccg tttactaaa ctaatcaac ggcgcgaag agtgaagtg aagaccgat cttcggctg gaagagaag aagaccgat aggaggaaa cggtttcgg agtcggacg ccgttaaag aaaccggac gttaagctt gccagtgaa agaaaaatc gaaatcaaa attcgaaat ccggcgaaa tatgaaaaa agcgagccg gaggaaaag gtgaggaag gtggaagtg gcggatgag aaaccggaa gagaaggtt gagtcgagt gtgactgaa ggtgatttg aggacgcaa ctgagtcgg aaacgagcc gagagacaa aaacatcct cttccggac ggcgtgccg tcgagactt ttgcagaat gcactacaa ggagttggc ttcaggaaa gtgaaaaag agtaaaaaa gacgaaaat acagaaggg aaattgccg attcacttg cgactgggg gctgccacc gagccgagt gggaaaaag tcgaggaag caaaagaaa ggaagtcag gagcaggtt taa
- >TC005835 RA
aaacacaga aattggagt cacaattaa acattgtga ctaatttaa ttaattgat atttttttg agttcggta tttccagtt taggtaaat aaactagtg acctgggcg ctgccaacc tcaccgatg gaaaattcc acagtaaat cgtcgaatt gtcagcgcc atattgttc acttattta tgttttgat ttcaatcga taaatagct aaaaaatcg taaaatgga agacaacac tcagttgtc ttttccaga agaggacga agatttgga agctttacg attagcagc cttacagtc tttgaaacg taaagccag taatgaagc ccaacaaat gggtcactt tggtggtat taaagacaa ggggaatca ttttaatgg gtcaccgcg aggcaagca caagagggg caaattttc aagactaca gcaaggtcg cattcctag aaacggcca gtttaacgg ttggtcacg aaattcgaa tttgatcgc cgttccaat ggtcacaga agacgagaa attaccgga aagtacgaa aaatgtgac tgaggatgt cccaaaatt ggtgcttcc ccaagacag gtattgcaa agcggacaa atcccccga acgttctga ccccatttc aaccaaatt cgaccgtta tgataactc agacgagtc agacgacga gtctgagga ggaacgttc caagtccga aacggagga aaagccaga gttgacccg ttcgaacag ccttgaggc cctgatggc cgaactcga aaatgaaat acaaggagt gacggcgga aaaatcgcc accaaaacc caagaaaaa acgaaaagt ttcggtcat cgaaccgga ggagaaaat cggcgaaaa cgccgttaa agaaaatgt gaaaccgga accggaggt gaaaaaatc accgaactt tcggcgcaa atggcaaaa gcctcgtcg gccgttcca tccgccgag tttagtgca tcccgtggc gccgccacc ggagatatt gccgcctcc ggtggtggc gtttgcccc gcctccaat tccgccgtt tgcgcctta tatgggttt tgatggtgt ttacgcccc gcctattta cgaatctcc gcctccaca gttgccacc gttgcatat ggcgccgcc tatttacga acgccctct ttctcccat tggtgttaa taccgattt tgggaatgg gatcatggc gccgctgtc gccacggag tgccgcttt tgtgatgga aaatcgggc tattattga gaagaggaa acgcaggtc gtactcccg gagtccgac tccggttag gttccggaa aagggcgtc tttatcgcc tttgaggcg ttccctatc tccatcacc tgtccgaaa agtatcgcc acgcctatc tccgaaaaa acaatcatc ggttcgtga acggttagg agcgaagaa cggcaagac tgaagaagt gaagaaaga ggaaaaaga agatccggt tttggaagc gcgacgaaa aaaatttga acgtaacga aatcacaat gactgaagg tgtgatacg tttgaagaa acccgaaag tgaggaagt gaagaagga ggaatgcgt cgaagacga gctggaggc ggaagaaat cgatgaaga tgcgttgtt ggaaggcgc cgattcgat tattttaga cccaaaact cgatacact tttctccga tgaagaatc ggacagtga caacgaagg gcggtttaa aaccaagca agaaacgaa tcagaaagt tccaatttt gccgtttac taaactaat caacggcgc gaagagtga agtgaagac cgatcttcg gctggaaga gaagaagac cgataggag gaaacggtt tcggagtcg gacgccgtt aaagaaacc ggacgttaa gcttgccag tgaaagaaa aatcgaaat caaaattcg aaatccggc gaaatatga aaaaagcga gccggagga aaaggtgag gaaggtgga agtggcgga tgagaaacc ggaagagaa ggttgagtc gagtgtgac tgaaggtga tttgaggac gcaactgag tcggaaacg agccgagag acaaaaaca tcctcttcc ggacggcgt gccgtcgag acttttgca gaatgcact acaaggagt tggcttcag gaaagtgaa aaagagtaa aaaagacga aaatacaga agggaaatt gccgattca cttgcgact gggggctgc caccgagcc gagtgggaa aaagtcgag gaagcaaaa gaaaggaag tcaggagca ggtattgtg ttttgttgc gtcatttct tattattat tatttttag tttaaaatt gttcgttag tacgttagt atcaaaacg atttctaat cggtgcact aacaaccaa acgtttcgc tttgaaaaa ccgcgtttt tattttcgt gactcatct gtgatttat aggttgcat gtgatgtat ttatttctc tcttttatg atttaatct gactgttac aggatatta tatcgacag acacacaaa aaaattaat tcgaattgt acaaactgt gatatttta taagacttg tacttaatt tagttattt atgtatagt atgcaattt ttaagtttg ttaattaag gtttaaaaa gtgcatacc agccatgta gatgttgta agtccctat ccctagtcc ttgtcgctc cttaaaaca tttagtgat cccaatgtt ccacgattg taacaattt ataatgcaa gaattacac atttgtgca gtgtatgtg gtcttaatg tgtaatttt tgtaagtga gacaatgat gtaactact ttgtatcat ttttgactg aaaagattg agaaaagaa agcacggga aaaaagaat gatacataa caaaacaaa acaaatcga aaaaatact tattggaaa tatgtttgt aacaatata ggtttaaac aactccaac aacgccaca aaggccaac ccacgaagg agtccgaat taatcagga agccgttag tgtgacatc aggactctc gttaattgt aataaagag taaataagt tgtaaatca ctttattct ttttttatt gcttgcttt atacatttt gtgactttt ttaagtatt taataaatg taaatattg tgtat
- >TC005835 RB
aaacacaga aattggagt cacaattaa acattgtga ctaatttaa ttaattgat atttttttg agttcggta tttccagtt taggtaaat aaactagtg acctgggcg ctgccaacc tcaccgatg gaaaattcc acagtaaat cgtcgaatt gtcagcgcc atattgttc acttattta tgttttgat ttcaatcga taaatagct aaaaaatcg taaaatgga agacaacac tcagttgtc ttttccaga agaggacga agatttgga agctttacg attagcagc cttacagtc tttgaaacg taaagccag taatgaagc ccaacaaat gggtcactt tggtggtat taaagacaa ggggaatca ttttaatgg gtcaccgcg aggcaagca caagagggg caaattttc aagactaca gcaaggtcg cattcctag aaacggcca gtttaacgg ttggtcacg aaattcgaa tttgatcgc cgttccaat ggtcacaga agacgagaa attaccgga aagtacgaa aaatgtgac tgaggatgt cccaaaatt ggtgcttcc ccaagacag gtattgcaa agcggacaa atcccccga acgttctga ccccatttc aaccaaatt cgaccgtta tgataactc agacgagtc agacgacga gtctgagga ggaacgttc caagtccga aacggagga aaagccaga gttgacccg ttcgaacag ccttgaggc cctgatggc cgaactcga aaatgaaat acaaggagt gacggcgga aaaatcgcc accaaaacc caagaaaaa acgaaaagt ttcggtcat cgaaccgga ggagaaaat cggcgaaaa cgccgttaa agaaaatgt gaaaccgga accggaggt gaaaaaatc accgaactt tcggcgcaa atggcaaaa gcctcgtcg gccgttcca tccgccgag tttagtgca tcccgtggc gccgccacc ggagatatt gccgcctcc ggtggtggc gtttgcccc gcctccaat tccgccgtt tgcgcctta tatgggttt tgatggtgt ttacgcccc gcctattta cgaatctcc gcctccaca gttgccacc gttgcatat ggcgccgcc tatttacga acgccctct ttctcccat tggtgttaa taccgattt tgggaatgg gatcatggc gccgctgtc gccacggag tgccgcttt tgtgatgga aaatcgggc tattattga gaagaggaa acgcaggtc gtactcccg gagtccgac tccggttag gttccggaa aagggcgtc tttatcgcc tttgaggcg ttccctatc tccatcacc tgtccgaaa agtatcgcc acgcctatc tccgaaaaa acaatcatc ggttcgtga acggttagg agcgaagaa cggcaagac tgaagaagt gaagaaaga ggaaaaaga agatccggt tttggaagc gcgacgaaa aaaatttga acgtaacga aatcacaat gactgaagg tgtgatacg tttgaagaa acccgaaag tgaggaagt gaagaagga ggaatgcgt cgaagacga gctggaggc ggaagaaat cgatgaaga tgcgttgtt ggaaggcgc cgattcgat tattttaga cccaaaact cgatacact tttctccga tgaagaatc ggacagtga caacgaagg gcggtttaa aaccaagca agaaacgaa tcagaaagt tccaatttt gccgtttac taaactaat caacggcgc gaagagtga agtgaagac cgatcttcg gctggaaga gaagaagac cgataggag gaaacggtt tcggagtcg gacgccgtt aaagaaacc ggacgttaa gcttgccag tgaaagaaa aatcgaaat caaaattcg aaatccggc gaaatatga aaaaagcga gccggagga aaaggtgag gaaggtgga agtggcgga tgagaaacc ggaagagaa ggttgagtc gagtgtgac tgaaggtga tttgaggac gcaactgag tcggaaacg agccgagag acaaaaaca tcctcttcc ggacggcgt gccgtcgag acttttgca gaatgcact acaaggagt tggcttcag gaaagtgaa aaagagtaa aaaagacga aaatacaga agggaaatt gccgattca cttgcgact gggggctgc caccgagcc gagtgggaa aaagtcgag gaagcaaaa gaaaggaag tcaggagca ggtttaaac aactccaac aacgccaca aaggccaac ccacgaagg agtccgaat taatcagga agccgttag tgtgacatc aggactctc gttaattgt aataaagag taaataagt tgtaaatca ctttattct ttttttatt gcttgcttt atacatttt gtgactttt ttaagtatt taataaatg taaatattg tgt
