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Gene information
General information
TC005835 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6979731..6987768
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005835
      iBeetle screen iB_00940

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Fertility
      tropharium structure irregular - Number of animals showing the phenotype: 1

      previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

      mature egg not present - Number of animals showing the phenotype: 4
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 20
      2 eggs
      embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      Comment: 2 out of 2
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005835 RA
        MEDNTQLSF PEEDEDLEA LRLAALQSL KRKASNEAQ 
        QMGHFGGIK DKGNHFNGS PRGKHKRGK FSRLQQGRI 
        PRNGQFNGW SRNSNLIAV PMVTEDEKL PESTKNVTE 
        DVPKLVLPQ DRYCKADKS PERSDPIST KFDRYDNSD 
        ESDDESEEE RSKSETEEK PELTRSNSL EALMAELEN 
        EIQGVTAEK SPPKPKKKR KVSVIEPEE KIGENAVKE 
        NVKPEPEVK KSPNFRRKW QKPRRPFHP PSLVHPVAP 
        PPEILPPPV VAFAPPPIP PFAPYMGFD GVYAPPIYE 
        SPPPQLPPL HMAPPIYER PLSPIGVNT DFGNGIMAP 
        LSPRSAAFV MENRAIIEK RKRRSYSRS PTPVRFRKR 
        ASLSPLRRS LSPSPVRKV SPRLSPKKQ SSVRERLGA 
        KNGKTEEVK KEEKEDPVL EARRKKFER NEITMTEGV 
        IRLKKPESE EVKKEECVE DELEAEEID EDALLEGAD 
        SIILDPKLD TLFSDEESD SDNEGRFKT KQETNQKVP 
        ILPFTKLIN GAKSEVKTD LRLEEKKTD RRKRFRSRT 
        PLKKPDVKL ASERKIEIK IRNPAKYEK SEPEEKVRK 
        VEVADEKPE EKVESSVTE GDLRTQLSR KRAERQKHP 
        LPDGVPSRL LQNALQGVG FRKVKKSKK DENTEGKLP 
        IHLRLGAAT EPSGKKSRK QKKGSQEQV LCFVASFLI 
        IIIFSLKLF VSTLVSKRF LIGALTTKR FALKNRVFI 
        FVTHL
      • >TC005835 RB
        MEDNTQLSF PEEDEDLEA LRLAALQSL KRKASNEAQ 
        QMGHFGGIK DKGNHFNGS PRGKHKRGK FSRLQQGRI 
        PRNGQFNGW SRNSNLIAV PMVTEDEKL PESTKNVTE 
        DVPKLVLPQ DRYCKADKS PERSDPIST KFDRYDNSD 
        ESDDESEEE RSKSETEEK PELTRSNSL EALMAELEN 
        EIQGVTAEK SPPKPKKKR KVSVIEPEE KIGENAVKE 
        NVKPEPEVK KSPNFRRKW QKPRRPFHP PSLVHPVAP 
        PPEILPPPV VAFAPPPIP PFAPYMGFD GVYAPPIYE 
        SPPPQLPPL HMAPPIYER PLSPIGVNT DFGNGIMAP 
        LSPRSAAFV MENRAIIEK RKRRSYSRS PTPVRFRKR 
        ASLSPLRRS LSPSPVRKV SPRLSPKKQ SSVRERLGA 
        KNGKTEEVK KEEKEDPVL EARRKKFER NEITMTEGV 
        IRLKKPESE EVKKEECVE DELEAEEID EDALLEGAD 
        SIILDPKLD TLFSDEESD SDNEGRFKT KQETNQKVP 
        ILPFTKLIN GAKSEVKTD LRLEEKKTD RRKRFRSRT 
        PLKKPDVKL ASERKIEIK IRNPAKYEK SEPEEKVRK 
        VEVADEKPE EKVESSVTE GDLRTQLSR KRAERQKHP 
        LPDGVPSRL LQNALQGVG FRKVKKSKK DENTEGKLP 
        IHLRLGAAT EPSGKKSRK QKKGSQEQV
      CDS sequence
      • >TC005835 RA
        atggaagac aacactcag ttgtctttt ccagaagag 
        gacgaagat ttggaagct ttacgatta gcagcctta 
        cagtctttg aaacgtaaa gccagtaat gaagcccaa 
        caaatgggt cactttggt ggtattaaa gacaagggg 
        aatcatttt aatgggtca ccgcgaggc aagcacaag 
        aggggcaaa ttttcaaga ctacagcaa ggtcgcatt 
        cctagaaac ggccagttt aacggttgg tcacgaaat 
        tcgaatttg atcgccgtt ccaatggtc acagaagac 
        gagaaatta ccggaaagt acgaaaaat gtgactgag 
        gatgtccca aaattggtg cttccccaa gacaggtat 
        tgcaaagcg gacaaatcc cccgaacgt tctgacccc 
        atttcaacc aaattcgac cgttatgat aactcagac 
        gagtcagac gacgagtct gaggaggaa cgttccaag 
        tccgaaacg gaggaaaag ccagagttg acccgttcg 
        aacagcctt gaggccctg atggccgaa ctcgaaaat 
        gaaatacaa ggagtgacg gcggaaaaa tcgccacca 
        aaacccaag aaaaaacga aaagtttcg gtcatcgaa 
        ccggaggag aaaatcggc gaaaacgcc gttaaagaa 
        aatgtgaaa ccggaaccg gaggtgaaa aaatcaccg 
        aactttcgg cgcaaatgg caaaagcct cgtcggccg 
        ttccatccg ccgagttta gtgcatccc gtggcgccg 
        ccaccggag atattgccg cctccggtg gtggcgttt 
        gccccgcct ccaattccg ccgtttgcg ccttatatg 
        ggttttgat ggtgtttac gccccgcct atttacgaa 
        tctccgcct ccacagttg ccaccgttg catatggcg 
        ccgcctatt tacgaacgc cctctttct cccattggt 
        gttaatacc gattttggg aatgggatc atggcgccg 
        ctgtcgcca cggagtgcc gcttttgtg atggaaaat 
        cgggctatt attgagaag aggaaacgc aggtcgtac 
        tcccggagt ccgactccg gttaggttc cggaaaagg 
        gcgtcttta tcgcctttg aggcgttcc ctatctcca 
        tcacctgtc cgaaaagta tcgccacgc ctatctccg 
        aaaaaacaa tcatcggtt cgtgaacgg ttaggagcg 
        aagaacggc aagactgaa gaagtgaag aaagaggaa 
        aaagaagat ccggttttg gaagcgcga cgaaaaaaa 
        tttgaacgt aacgaaatc acaatgact gaaggtgtg 
        atacgtttg aagaaaccc gaaagtgag gaagtgaag 
        aaggaggaa tgcgtcgaa gacgagctg gaggcggaa 
        gaaatcgat gaagatgcg ttgttggaa ggcgccgat 
        tcgattatt ttagaccca aaactcgat acacttttc 
        tccgatgaa gaatcggac agtgacaac gaagggcgg 
        tttaaaacc aagcaagaa acgaatcag aaagttcca 
        attttgccg tttactaaa ctaatcaac ggcgcgaag 
        agtgaagtg aagaccgat cttcggctg gaagagaag 
        aagaccgat aggaggaaa cggtttcgg agtcggacg 
        ccgttaaag aaaccggac gttaagctt gccagtgaa 
        agaaaaatc gaaatcaaa attcgaaat ccggcgaaa 
        tatgaaaaa agcgagccg gaggaaaag gtgaggaag 
        gtggaagtg gcggatgag aaaccggaa gagaaggtt 
        gagtcgagt gtgactgaa ggtgatttg aggacgcaa 
        ctgagtcgg aaacgagcc gagagacaa aaacatcct 
        cttccggac ggcgtgccg tcgagactt ttgcagaat 
        gcactacaa ggagttggc ttcaggaaa gtgaaaaag 
        agtaaaaaa gacgaaaat acagaaggg aaattgccg 
        attcacttg cgactgggg gctgccacc gagccgagt 
        gggaaaaag tcgaggaag caaaagaaa ggaagtcag 
        gagcaggta ttgtgtttt gttgcgtca tttcttatt 
        attattatt tttagttta aaattgttc gttagtacg 
        ttagtatca aaacgattt ctaatcggt gcactaaca 
        accaaacgt ttcgctttg aaaaaccgc gtttttatt 
        ttcgtgact catctgtga
      • >TC005835 RB
        atggaagac aacactcag ttgtctttt ccagaagag 
        gacgaagat ttggaagct ttacgatta gcagcctta 
        cagtctttg aaacgtaaa gccagtaat gaagcccaa 
        caaatgggt cactttggt ggtattaaa gacaagggg 
        aatcatttt aatgggtca ccgcgaggc aagcacaag 
        aggggcaaa ttttcaaga ctacagcaa ggtcgcatt 
        cctagaaac ggccagttt aacggttgg tcacgaaat 
        tcgaatttg atcgccgtt ccaatggtc acagaagac 
        gagaaatta ccggaaagt acgaaaaat gtgactgag 
        gatgtccca aaattggtg cttccccaa gacaggtat 
        tgcaaagcg gacaaatcc cccgaacgt tctgacccc 
        atttcaacc aaattcgac cgttatgat aactcagac 
        gagtcagac gacgagtct gaggaggaa cgttccaag 
        tccgaaacg gaggaaaag ccagagttg acccgttcg 
        aacagcctt gaggccctg atggccgaa ctcgaaaat 
        gaaatacaa ggagtgacg gcggaaaaa tcgccacca 
        aaacccaag aaaaaacga aaagtttcg gtcatcgaa 
        ccggaggag aaaatcggc gaaaacgcc gttaaagaa 
        aatgtgaaa ccggaaccg gaggtgaaa aaatcaccg 
        aactttcgg cgcaaatgg caaaagcct cgtcggccg 
        ttccatccg ccgagttta gtgcatccc gtggcgccg 
        ccaccggag atattgccg cctccggtg gtggcgttt 
        gccccgcct ccaattccg ccgtttgcg ccttatatg 
        ggttttgat ggtgtttac gccccgcct atttacgaa 
        tctccgcct ccacagttg ccaccgttg catatggcg 
        ccgcctatt tacgaacgc cctctttct cccattggt 
        gttaatacc gattttggg aatgggatc atggcgccg 
        ctgtcgcca cggagtgcc gcttttgtg atggaaaat 
        cgggctatt attgagaag aggaaacgc aggtcgtac 
        tcccggagt ccgactccg gttaggttc cggaaaagg 
        gcgtcttta tcgcctttg aggcgttcc ctatctcca 
        tcacctgtc cgaaaagta tcgccacgc ctatctccg 
        aaaaaacaa tcatcggtt cgtgaacgg ttaggagcg 
        aagaacggc aagactgaa gaagtgaag aaagaggaa 
        aaagaagat ccggttttg gaagcgcga cgaaaaaaa 
        tttgaacgt aacgaaatc acaatgact gaaggtgtg 
        atacgtttg aagaaaccc gaaagtgag gaagtgaag 
        aaggaggaa tgcgtcgaa gacgagctg gaggcggaa 
        gaaatcgat gaagatgcg ttgttggaa ggcgccgat 
        tcgattatt ttagaccca aaactcgat acacttttc 
        tccgatgaa gaatcggac agtgacaac gaagggcgg 
        tttaaaacc aagcaagaa acgaatcag aaagttcca 
        attttgccg tttactaaa ctaatcaac ggcgcgaag 
        agtgaagtg aagaccgat cttcggctg gaagagaag 
        aagaccgat aggaggaaa cggtttcgg agtcggacg 
        ccgttaaag aaaccggac gttaagctt gccagtgaa 
        agaaaaatc gaaatcaaa attcgaaat ccggcgaaa 
        tatgaaaaa agcgagccg gaggaaaag gtgaggaag 
        gtggaagtg gcggatgag aaaccggaa gagaaggtt 
        gagtcgagt gtgactgaa ggtgatttg aggacgcaa 
        ctgagtcgg aaacgagcc gagagacaa aaacatcct 
        cttccggac ggcgtgccg tcgagactt ttgcagaat 
        gcactacaa ggagttggc ttcaggaaa gtgaaaaag 
        agtaaaaaa gacgaaaat acagaaggg aaattgccg 
        attcacttg cgactgggg gctgccacc gagccgagt 
        gggaaaaag tcgaggaag caaaagaaa ggaagtcag 
        gagcaggtt taa
      mRNA sequence
      • >TC005835 RA
        aaacacaga aattggagt cacaattaa acattgtga 
        ctaatttaa ttaattgat atttttttg agttcggta 
        tttccagtt taggtaaat aaactagtg acctgggcg 
        ctgccaacc tcaccgatg gaaaattcc acagtaaat 
        cgtcgaatt gtcagcgcc atattgttc acttattta 
        tgttttgat ttcaatcga taaatagct aaaaaatcg 
        taaaatgga agacaacac tcagttgtc ttttccaga 
        agaggacga agatttgga agctttacg attagcagc 
        cttacagtc tttgaaacg taaagccag taatgaagc 
        ccaacaaat gggtcactt tggtggtat taaagacaa 
        ggggaatca ttttaatgg gtcaccgcg aggcaagca 
        caagagggg caaattttc aagactaca gcaaggtcg 
        cattcctag aaacggcca gtttaacgg ttggtcacg 
        aaattcgaa tttgatcgc cgttccaat ggtcacaga 
        agacgagaa attaccgga aagtacgaa aaatgtgac 
        tgaggatgt cccaaaatt ggtgcttcc ccaagacag 
        gtattgcaa agcggacaa atcccccga acgttctga 
        ccccatttc aaccaaatt cgaccgtta tgataactc 
        agacgagtc agacgacga gtctgagga ggaacgttc 
        caagtccga aacggagga aaagccaga gttgacccg 
        ttcgaacag ccttgaggc cctgatggc cgaactcga 
        aaatgaaat acaaggagt gacggcgga aaaatcgcc 
        accaaaacc caagaaaaa acgaaaagt ttcggtcat 
        cgaaccgga ggagaaaat cggcgaaaa cgccgttaa 
        agaaaatgt gaaaccgga accggaggt gaaaaaatc 
        accgaactt tcggcgcaa atggcaaaa gcctcgtcg 
        gccgttcca tccgccgag tttagtgca tcccgtggc 
        gccgccacc ggagatatt gccgcctcc ggtggtggc 
        gtttgcccc gcctccaat tccgccgtt tgcgcctta 
        tatgggttt tgatggtgt ttacgcccc gcctattta 
        cgaatctcc gcctccaca gttgccacc gttgcatat 
        ggcgccgcc tatttacga acgccctct ttctcccat 
        tggtgttaa taccgattt tgggaatgg gatcatggc 
        gccgctgtc gccacggag tgccgcttt tgtgatgga 
        aaatcgggc tattattga gaagaggaa acgcaggtc 
        gtactcccg gagtccgac tccggttag gttccggaa 
        aagggcgtc tttatcgcc tttgaggcg ttccctatc 
        tccatcacc tgtccgaaa agtatcgcc acgcctatc 
        tccgaaaaa acaatcatc ggttcgtga acggttagg 
        agcgaagaa cggcaagac tgaagaagt gaagaaaga 
        ggaaaaaga agatccggt tttggaagc gcgacgaaa 
        aaaatttga acgtaacga aatcacaat gactgaagg 
        tgtgatacg tttgaagaa acccgaaag tgaggaagt 
        gaagaagga ggaatgcgt cgaagacga gctggaggc 
        ggaagaaat cgatgaaga tgcgttgtt ggaaggcgc 
        cgattcgat tattttaga cccaaaact cgatacact 
        tttctccga tgaagaatc ggacagtga caacgaagg 
        gcggtttaa aaccaagca agaaacgaa tcagaaagt 
        tccaatttt gccgtttac taaactaat caacggcgc 
        gaagagtga agtgaagac cgatcttcg gctggaaga 
        gaagaagac cgataggag gaaacggtt tcggagtcg 
        gacgccgtt aaagaaacc ggacgttaa gcttgccag 
        tgaaagaaa aatcgaaat caaaattcg aaatccggc 
        gaaatatga aaaaagcga gccggagga aaaggtgag 
        gaaggtgga agtggcgga tgagaaacc ggaagagaa 
        ggttgagtc gagtgtgac tgaaggtga tttgaggac 
        gcaactgag tcggaaacg agccgagag acaaaaaca 
        tcctcttcc ggacggcgt gccgtcgag acttttgca 
        gaatgcact acaaggagt tggcttcag gaaagtgaa 
        aaagagtaa aaaagacga aaatacaga agggaaatt 
        gccgattca cttgcgact gggggctgc caccgagcc 
        gagtgggaa aaagtcgag gaagcaaaa gaaaggaag 
        tcaggagca ggtattgtg ttttgttgc gtcatttct 
        tattattat tatttttag tttaaaatt gttcgttag 
        tacgttagt atcaaaacg atttctaat cggtgcact 
        aacaaccaa acgtttcgc tttgaaaaa ccgcgtttt 
        tattttcgt gactcatct gtgatttat aggttgcat 
        gtgatgtat ttatttctc tcttttatg atttaatct 
        gactgttac aggatatta tatcgacag acacacaaa 
        aaaattaat tcgaattgt acaaactgt gatatttta 
        taagacttg tacttaatt tagttattt atgtatagt 
        atgcaattt ttaagtttg ttaattaag gtttaaaaa 
        gtgcatacc agccatgta gatgttgta agtccctat 
        ccctagtcc ttgtcgctc cttaaaaca tttagtgat 
        cccaatgtt ccacgattg taacaattt ataatgcaa 
        gaattacac atttgtgca gtgtatgtg gtcttaatg 
        tgtaatttt tgtaagtga gacaatgat gtaactact 
        ttgtatcat ttttgactg aaaagattg agaaaagaa 
        agcacggga aaaaagaat gatacataa caaaacaaa 
        acaaatcga aaaaatact tattggaaa tatgtttgt 
        aacaatata ggtttaaac aactccaac aacgccaca 
        aaggccaac ccacgaagg agtccgaat taatcagga 
        agccgttag tgtgacatc aggactctc gttaattgt 
        aataaagag taaataagt tgtaaatca ctttattct 
        ttttttatt gcttgcttt atacatttt gtgactttt 
        ttaagtatt taataaatg taaatattg tgtat
      • >TC005835 RB
        aaacacaga aattggagt cacaattaa acattgtga 
        ctaatttaa ttaattgat atttttttg agttcggta 
        tttccagtt taggtaaat aaactagtg acctgggcg 
        ctgccaacc tcaccgatg gaaaattcc acagtaaat 
        cgtcgaatt gtcagcgcc atattgttc acttattta 
        tgttttgat ttcaatcga taaatagct aaaaaatcg 
        taaaatgga agacaacac tcagttgtc ttttccaga 
        agaggacga agatttgga agctttacg attagcagc 
        cttacagtc tttgaaacg taaagccag taatgaagc 
        ccaacaaat gggtcactt tggtggtat taaagacaa 
        ggggaatca ttttaatgg gtcaccgcg aggcaagca 
        caagagggg caaattttc aagactaca gcaaggtcg 
        cattcctag aaacggcca gtttaacgg ttggtcacg 
        aaattcgaa tttgatcgc cgttccaat ggtcacaga 
        agacgagaa attaccgga aagtacgaa aaatgtgac 
        tgaggatgt cccaaaatt ggtgcttcc ccaagacag 
        gtattgcaa agcggacaa atcccccga acgttctga 
        ccccatttc aaccaaatt cgaccgtta tgataactc 
        agacgagtc agacgacga gtctgagga ggaacgttc 
        caagtccga aacggagga aaagccaga gttgacccg 
        ttcgaacag ccttgaggc cctgatggc cgaactcga 
        aaatgaaat acaaggagt gacggcgga aaaatcgcc 
        accaaaacc caagaaaaa acgaaaagt ttcggtcat 
        cgaaccgga ggagaaaat cggcgaaaa cgccgttaa 
        agaaaatgt gaaaccgga accggaggt gaaaaaatc 
        accgaactt tcggcgcaa atggcaaaa gcctcgtcg 
        gccgttcca tccgccgag tttagtgca tcccgtggc 
        gccgccacc ggagatatt gccgcctcc ggtggtggc 
        gtttgcccc gcctccaat tccgccgtt tgcgcctta 
        tatgggttt tgatggtgt ttacgcccc gcctattta 
        cgaatctcc gcctccaca gttgccacc gttgcatat 
        ggcgccgcc tatttacga acgccctct ttctcccat 
        tggtgttaa taccgattt tgggaatgg gatcatggc 
        gccgctgtc gccacggag tgccgcttt tgtgatgga 
        aaatcgggc tattattga gaagaggaa acgcaggtc 
        gtactcccg gagtccgac tccggttag gttccggaa 
        aagggcgtc tttatcgcc tttgaggcg ttccctatc 
        tccatcacc tgtccgaaa agtatcgcc acgcctatc 
        tccgaaaaa acaatcatc ggttcgtga acggttagg 
        agcgaagaa cggcaagac tgaagaagt gaagaaaga 
        ggaaaaaga agatccggt tttggaagc gcgacgaaa 
        aaaatttga acgtaacga aatcacaat gactgaagg 
        tgtgatacg tttgaagaa acccgaaag tgaggaagt 
        gaagaagga ggaatgcgt cgaagacga gctggaggc 
        ggaagaaat cgatgaaga tgcgttgtt ggaaggcgc 
        cgattcgat tattttaga cccaaaact cgatacact 
        tttctccga tgaagaatc ggacagtga caacgaagg 
        gcggtttaa aaccaagca agaaacgaa tcagaaagt 
        tccaatttt gccgtttac taaactaat caacggcgc 
        gaagagtga agtgaagac cgatcttcg gctggaaga 
        gaagaagac cgataggag gaaacggtt tcggagtcg 
        gacgccgtt aaagaaacc ggacgttaa gcttgccag 
        tgaaagaaa aatcgaaat caaaattcg aaatccggc 
        gaaatatga aaaaagcga gccggagga aaaggtgag 
        gaaggtgga agtggcgga tgagaaacc ggaagagaa 
        ggttgagtc gagtgtgac tgaaggtga tttgaggac 
        gcaactgag tcggaaacg agccgagag acaaaaaca 
        tcctcttcc ggacggcgt gccgtcgag acttttgca 
        gaatgcact acaaggagt tggcttcag gaaagtgaa 
        aaagagtaa aaaagacga aaatacaga agggaaatt 
        gccgattca cttgcgact gggggctgc caccgagcc 
        gagtgggaa aaagtcgag gaagcaaaa gaaaggaag 
        tcaggagca ggtttaaac aactccaac aacgccaca 
        aaggccaac ccacgaagg agtccgaat taatcagga 
        agccgttag tgtgacatc aggactctc gttaattgt 
        aataaagag taaataagt tgtaaatca ctttattct 
        ttttttatt gcttgcttt atacatttt gtgactttt 
        ttaagtatt taataaatg taaatattg tgt
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