Status
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Gene information
General information
TC005830 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7107372..7118309
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005830
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00939

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005830 RA
      MSLPGNGIF VVRGEMCTL MTAMRRGAR WSSHSHQDD 
      DEPLMKNFQ ELKEILNKI DDLRLVQPS IYLGPFLEV 
      IRSEETTGP VTSLALSAV NKFLAYGLI DPTHSSVPA 
      TVHSIADAV THARFVGTD QSSDGVVLM RILQVLRTL 
      TLAPEGATL TNESLCEIM LSCFRICFE TRLNELLRR 
      TAEHYLKDM VQLVFMRLP QFSDDLCAV KQFKMRPGA 
      IEQTRTKRK KSFRLSKSL EETPSPTTQ LKPQNHSNH 
      LSTTPMTPA GNIVDMQGS ISQNTPENA AESPSNFSE 
      TGESNVQIS VDPPSPEEN HEEEATPEV EETQDYVNQ 
      RGIRFTQQM GEEVVLIPY GLACVRELF RFLISLCNP 
      LDKQNTDVM IHLGLTLLT VAFEVGADS IGKYSPLLA 
      LVKDDLCRN LFSLLTSER LSVFAADLQ VCFLMFEAL 
      RTHLKYQLE FYLTKLIDI IVTDSGKIS YEHKEIALD 
      NILQLWRIP GLVTELYLN YDCNMYCTN LYEDLTKLL 
      AKNAFSATS GVYHTHMLS LDALLTVIE SIEQHCFEK 
      SESEEGVKV ESSTENIES ITNFIGKTT RQKISDQIP 
      SKDELMAQK NIKKWLPTG TDHFNHKPK KGIQFLQEH 
      GVLKSELDP HEIALFLKE NSGLDKKMI GEFLGNRSN 
      VAILDAFLK TFDFTNTRI DEALRHYLE TFRLPGEAP 
      IISLLMEHF AEHWHKSNG EPFADVDAA FTLAYAVII 
      LNVDQHNQN AKKQKTPMT LAGFKKNLK GVNGGNDFD 
      EDMLDEIYN AIRTDEIVM PAEQTGLVR ENYLWKVLL 
      RKGASKDGV YYHLNGGQF DQELFQLIW GPIVAALSF 
      VFDKSEEQL IYKKAMTGF QKCAFVSSH FAISKNLDM 
      LIQTLAKFT NFHNLQRPN NGIIMFGAN IKARLALKC 
      VLDLCHQHG DNIREGWKN LFDLVLSLY VLGLLPRSY 
      VEAEDFIES SGKFNLVYE EVENLQKQE SGLFSSLYS 
      YMVSSENLS KVPTVEEQQ HIDVAKETI RECNFDLVI 
      TDSKFLHDE SLKALVGAL VELSRPPDV QKSLGYNYN 
      ENVAVFFLE LLIKIVIQN RDRVMTIWQ TVRDHIYTL 
      VMNSSVFDY QFLLERSVI GLLRIAIRL MRNEDMSPI 
      VLQSLQMLL LLKSSTLCR ISRQISFGL YELLKTSAQ 
      NIHTETDWT IIFTLLECV GAGAVPPKA VADDHPTTK 
      SDGEGVASD DDTPERGYT SDSELSKSP KHRPQSPAC 
      GWILVGNEG EIQPVVGRT LPPSQYSLA LERNLGPHD 
      PSSLIKCCE SLAFLVRDV AHITPYNFD DCVHCIRTF 
      VEASLHGNR RQRRGKGRR QRRKGAERR SPTSSPDED 
      SDEEEVPSG YHQISIQLL DLMHTLHTR TAQIFKWWA 
      EEGGALAKE TSLWTQGWC PLLQGIARL CCDVRREIR 
      MSAITYLQR ALLVHDLQT LTGPEWEAC FHRVLFPLL 
      AHLLSNIDP KDPLAMEET RMRAATVLS KVFLHHLTP 
      LLSLPTFSN LWLIILDFI DKYMHADKS DLLAEAIPE 
      SLKNMLLVM DSAKVFDGP DGKSPLWAA TWDRINKFL 
      PGMKEELFR EQQGKRENV EEVVQHNIE NATRSIILQ 
      PPTSQQQVS SPLFAHLGQ MVSTPIASA LPPPPPPFL 
      QPTLQPGMP YLYAKNQSH TFPVLYNSP VSLYSEYVG 
      NPYNNAAPE GATAEENTQ SLLDVNKNN DLVEADAPN 
      NNTNVFQSS NYFSADSGG DFIPPGSEI LFGTEQGNF 
      IPACTNSDI KTSDV
    CDS sequence
    • >TC005830 RA
      atgtcgctt ccgggcaac ggtattttc gtcgtaagg 
      ggtgaaatg tgcacgtta atgacggcg atgcgccgt 
      ggcgcccgc tggtcgtcc cactctcac caagacgac 
      gacgaacct cttatgaaa aacttccaa gaactgaag 
      gaaatcctg aacaaaatc gacgacttg cgtctggtg 
      caaccaagc atctacttg gggccgttc ctggaggtg 
      atccgatcg gaggaaacc actggtccg gtcacaagt 
      ctcgcgttg tccgccgtt aacaaattc ctagcgtat 
      ggattgatc gaccccacg cacagctcg gtgcccgcc 
      accgtgcac agcatcgcc gacgcggtc acgcacgcc 
      cgcttcgtg ggcaccgac cagagctcc gacggggtg 
      gttttaatg cggattttg caagtcttg cggactctt 
      acactggcc cccgagggg gccacgctc accaacgag 
      agtctctgc gagatcatg ctgtcgtgc ttcaggatt 
      tgtttcgag acacgtttg aacgaattg ttgagacgc 
      accgccgaa cactatctc aaagacatg gtccagctt 
      gtctttatg cgcctccct cagttttcc gatgacctg 
      tgtgccgtc aaacaattc aagatgagg ccgggggct 
      attgaacag acccggacg aagcggaaa aagtcgttc 
      cgactgtcg aaaagtttg gaggagacg ccgtctccc 
      acgacccag ctgaagccc caaaatcac agtaaccac 
      ctttcgacc acccccatg acccccgcg gggaatatc 
      gtggacatg caggggtca atttcgcaa aataccccc 
      gaaaatgcg gcagaatcg ccctcgaat ttttccgaa 
      actggggaa agtaacgtg caaataagt gtggatccg 
      ccaagtccg gaggaaaat cacgaggag gaagcgacg 
      cctgaagtt gaagagact caggattat gtcaaccag 
      agggggatc aggttcacg caacagatg ggcgaggag 
      gttgtgttg attccttac ggcctagca tgtgtgcgt 
      gaattgttt aggtttttg atcagcctg tgtaacccg 
      ttagataag caaaacacc gatgttatg attcatttg 
      gggctcacg ttgctcact gtggccttt gaagtgggg 
      gctgacagt atcggcaaa tattcaccg cttctggct 
      ctagtcaag gatgatttg tgtcggaac ttattctct 
      ctgttgacg tcggaacgc ttgtcggtc ttcgcggcc 
      gatttacaa gtctgtttt ttaatgttc gaggcgttg 
      cggacgcac ctcaagtac cagctagag ttctacttg 
      accaaacta attgatatc attgtgacc gattcgggg 
      aaaatctcc tacgaacat aaggaaatc gcccttgat 
      aatatcctt caactgtgg cgaattccg ggtcttgtg 
      actgagctc tacctaaat tatgactgt aatatgtat 
      tgtacaaat ctctacgag gatttgact aaactgttg 
      gcgaaaaac gcattttcg gccacgtct ggcgtttat 
      cacacgcat atgttatca ctggacgcg ctattaacg 
      gttatagag agcatagaa caacattgt ttcgagaaa 
      agtgagtcg gaagaaggg gttaaagtc gagtcaagc 
      accgaaaat atcgaatcc atcacgaat tttattggt 
      aagacgaca agacagaaa atctctgat caaattccc 
      agcaaggac gaactcatg gcccaaaaa aacataaaa 
      aaatggctc ccaaccgga acggaccac ttcaaccac 
      aagcccaaa aaaggcatc cagttcctg caagagcac 
      ggcgtattg aaaagcgag ctggacccg cacgaaatt 
      gcgcttttc ctcaaggaa aattccgga ctggacaag 
      aaaatgatt ggggaattt ttaggaaat cgtagtaat 
      gtcgcaatt cttgatgcg tttctgaaa acttttgat 
      tttacaaat acacgaatc gatgaagct ttacgacac 
      tatttggag acttttaga ttgccgggg gaagccccc 
      attatttcg ctcctgatg gaacatttt gcggagcac 
      tggcataaa agtaacggc gagccgttt gccgacgtc 
      gatgcagct ttcacactc gcctacgct gtgattata 
      ctaaacgtg gaccaacat aatcaaaat gcgaaaaaa 
      cagaaaact ccaatgaca cttgccggg tttaagaaa 
      aatctcaaa ggggttaac ggaggcaac gattttgat 
      gaggatatg ttagacgag atttacaac gcgattagg 
      actgacgaa attgtgatg ccggctgag cagacgggc 
      ctcgtccgt gaaaactac ctctggaag gtccttttg 
      cgcaaagga gcctccaaa gacggtgtc tattaccac 
      ttaaacggg ggtcagttt gaccaggaa ctgtttcaa 
      ttaatttgg ggccccatt gtggccgcc ctgtctttt 
      gttttcgac aaatcagag gagcaactg atttacaaa 
      aaagcaatg acaggattc cagaaatgc gcatttgtt 
      tcgtcacac tttgcaatt tcgaaaaat ctcgacatg 
      ttgatacaa accctagcc aaattcacc aacttccac 
      aatttgcaa agaccgaac aacggcatt atcatgttt 
      ggggccaat attaaggcg cgtctggcc ttgaaatgc 
      gtccttgac ttgtgccac caacacggc gataacatt 
      cgagaaggc tggaagaat ttattcgat ttggttttg 
      tcgttgtac gtgttgggt ttgttgccg agaagttac 
      gtcgaagct gaggatttt atcgaaagt agcgggaaa 
      ttcaatttg gtttatgag gaggtggaa aatttgcaa 
      aaacaagaa agcgggctt tttagttcg ttgtactcc 
      tacatggtt tcgagcgag aatttatca aaagtacca 
      actgttgaa gaacaacaa cacatcgat gtggcgaaa 
      gagacgata agggagtgc aactttgat ttggttata 
      accgattcg aagtttctt cacgacgag tcacttaag 
      gcccttgtt ggggctttg gtcgagctg tcgaggccc 
      ccagacgtc caaaagagc ctcgggtat aattacaat 
      gaaaacgtc gccgtgttt ttcctcgaa cttttgatc 
      aaaattgtg atacaaaac cgggatcga gtcatgaca 
      atatggcag accgtccgc gaccatatc tacaccctc 
      gtcatgaac tcctcagtc ttcgactac caatttcta 
      ctcgaacga tcagtcatc ggcctcctg cgcattgca 
      attcgtcta atgcgcaac gaagacatg agccccatc 
      gtcctccaa tccctccag atgctcctc ctcctcaaa 
      tcctccact ctgtgtcga atttcgcgc caaatctcg 
      ttcggcctc tacgaactc cttaaaacc tccgcccag 
      aacattcac accgagaca gactggacg attattttc 
      accctattg gagtgcgtg ggggcgggc gctgtccct 
      cccaaagca gtcgccgac gaccacccc accaccaaa 
      tccgacggc gaaggcgtg gcttccgac gacgacacg 
      cccgaacgt ggctacacc tccgactcc gaactaagc 
      aaaagcccg aagcaccgc ccacaaagc cccgcctgt 
      ggctggata ctggtgggt aacgaaggc gagattcaa 
      ccagtagtg gggcgtacc ctccctcca agtcagtac 
      agtctcgcc cttgaacgt aatttgggg ccgcatgac 
      ccgagcagt ttgatcaaa tgttgcgaa agtcttgcg 
      tttctggtc agggatgtg gctcatatc acgccctat 
      aatttcgac gattgcgtt cactgtatt aggacgttt 
      gtggaggcc agtctccat ggtaatagg agacaaagg 
      aggggtaag gggcggaga cagaggcgg aaaggggcg 
      gagcgccga agtccgacg agcagtccg gacgaggat 
      agtgacgag gaggaggtg cctagtggc tatcaccaa 
      atttcgata caattgtta gatttgatg cacaccctg 
      catactaga accgcccag attttcaaa tggtgggcg 
      gaggaaggc ggggctttg gccaaagaa acgtcgttg 
      tggacgcaa ggctggtgc ccgttgctt caaggcatt 
      gccaggctg tgttgcgac gtcaggaga gagatccgc 
      atgagcgca attacatac ctgcagcga gcgctcctg 
      gtccacgac ttgcagacg ctcacgggc cccgaatgg 
      gaggcgtgt ttccaccgc gtcctcttc ccccttctg 
      gcacacctc ttgagtaac attgacccc aaggacccc 
      ctcgccatg gaggagact cgcatgcgc gccgccacc 
      gtcctctcc aaagtgttc ctccaccac ctaactcca 
      ctcctctcc ctcccaacc ttctccaat ctatggctg 
      attatcctg gacttcatt gacaagtac atgcacgcc 
      gacaagagc gatctcctg gcggaagcc atcccagaa 
      tcgctcaaa aacatgctc ctggtgatg gactcggcg 
      aaggttttt gacggaccg gacggcaag agcccgctc 
      tgggccgcc acgtgggac agaattaac aaattcctc 
      ccagggatg aaggaggag ttgttcagg gagcagcag 
      gggaagcgg gagaacgtg gaggaggtc gtgcagcac 
      aatatcgag aacgcgacc aggtcgatc atcctgcag 
      ccccccacg agccaacag caagtctca tcgcccctt 
      tttgcgcat ttggggcag atggtttcg acgccgatt 
      gcctcggcg ctgccgccg ccaccgccc ccgttcctc 
      cagccgacg ctccagccc ggcatgccc tacttatac 
      gccaaaaat caaagtcac acatttcca gtgttgtac 
      aacagtccg gtatcgttg tactccgaa tatgtggga 
      aacccctac aataatgcc gctccggag ggggccaca 
      gctgaggaa aatacgcag agtttgtta gatgttaat 
      aaaaataac gatctagtc gaggctgac gccccaaat 
      aacaacacc aacgtgttt cagtcttca aattatttc 
      agtgcggac tcgggcggc gactttata cccccgggc 
      tccgagatt ctgtttggt acagaacag ggaaacttc 
      attccagcg tgtacaaat agcgacata aaaacgagc 
      gatgtttaa
    mRNA sequence
    • >TC005830 RA
      gtagtgttt cgcgaaacg tcaggctga cgtgttggt 
      ggtgtcagt atgtaaaca gtgaaaatc gtgaatttt 
      gaaaaaacg ggcaaaaat gtcgcttcc gggcaacgg 
      tattttcgt cgtaagggg tgaaatgtg cacgttaat 
      gacggcgat gcgccgtgg cgcccgctg gtcgtccca 
      ctctcacca agacgacga cgaacctct tatgaaaaa 
      cttccaaga actgaagga aatcctgaa caaaatcga 
      cgacttgcg tctggtgca accaagcat ctacttggg 
      gccgttcct ggaggtgat ccgatcgga ggaaaccac 
      tggtccggt cacaagtct cgcgttgtc cgccgttaa 
      caaattcct agcgtatgg attgatcga ccccacgca 
      cagctcggt gcccgccac cgtgcacag catcgccga 
      cgcggtcac gcacgcccg cttcgtggg caccgacca 
      gagctccga cggggtggt tttaatgcg gattttgca 
      agtcttgcg gactcttac actggcccc cgagggggc 
      cacgctcac caacgagag tctctgcga gatcatgct 
      gtcgtgctt caggatttg tttcgagac acgtttgaa 
      cgaattgtt gagacgcac cgccgaaca ctatctcaa 
      agacatggt ccagcttgt ctttatgcg cctccctca 
      gttttccga tgacctgtg tgccgtcaa acaattcaa 
      gatgaggcc gggggctat tgaacagac ccggacgaa 
      gcggaaaaa gtcgttccg actgtcgaa aagtttgga 
      ggagacgcc gtctcccac gacccagct gaagcccca 
      aaatcacag taaccacct ttcgaccac ccccatgac 
      ccccgcggg gaatatcgt ggacatgca ggggtcaat 
      ttcgcaaaa tacccccga aaatgcggc agaatcgcc 
      ctcgaattt ttccgaaac tggggaaag taacgtgca 
      aataagtgt ggatccgcc aagtccgga ggaaaatca 
      cgaggagga agcgacgcc tgaagttga agagactca 
      ggattatgt caaccagag ggggatcag gttcacgca 
      acagatggg cgaggaggt tgtgttgat tccttacgg 
      cctagcatg tgtgcgtga attgtttag gtttttgat 
      cagcctgtg taacccgtt agataagca aaacaccga 
      tgttatgat tcatttggg gctcacgtt gctcactgt 
      ggcctttga agtgggggc tgacagtat cggcaaata 
      ttcaccgct tctggctct agtcaagga tgatttgtg 
      tcggaactt attctctct gttgacgtc ggaacgctt 
      gtcggtctt cgcggccga tttacaagt ctgtttttt 
      aatgttcga ggcgttgcg gacgcacct caagtacca 
      gctagagtt ctacttgac caaactaat tgatatcat 
      tgtgaccga ttcggggaa aatctccta cgaacataa 
      ggaaatcgc ccttgataa tatccttca actgtggcg 
      aattccggg tcttgtgac tgagctcta cctaaatta 
      tgactgtaa tatgtattg tacaaatct ctacgagga 
      tttgactaa actgttggc gaaaaacgc attttcggc 
      cacgtctgg cgtttatca cacgcatat gttatcact 
      ggacgcgct attaacggt tatagagag catagaaca 
      acattgttt cgagaaaag tgagtcgga agaaggggt 
      taaagtcga gtcaagcac cgaaaatat cgaatccat 
      cacgaattt tattggtaa gacgacaag acagaaaat 
      ctctgatca aattcccag caaggacga actcatggc 
      ccaaaaaaa cataaaaaa atggctccc aaccggaac 
      ggaccactt caaccacaa gcccaaaaa aggcatcca 
      gttcctgca agagcacgg cgtattgaa aagcgagct 
      ggacccgca cgaaattgc gcttttcct caaggaaaa 
      ttccggact ggacaagaa aatgattgg ggaattttt 
      aggaaatcg tagtaatgt cgcaattct tgatgcgtt 
      tctgaaaac ttttgattt tacaaatac acgaatcga 
      tgaagcttt acgacacta tttggagac ttttagatt 
      gccggggga agcccccat tatttcgct cctgatgga 
      acattttgc ggagcactg gcataaaag taacggcga 
      gccgtttgc cgacgtcga tgcagcttt cacactcgc 
      ctacgctgt gattatact aaacgtgga ccaacataa 
      tcaaaatgc gaaaaaaca gaaaactcc aatgacact 
      tgccgggtt taagaaaaa tctcaaagg ggttaacgg 
      aggcaacga ttttgatga ggatatgtt agacgagat 
      ttacaacgc gattaggac tgacgaaat tgtgatgcc 
      ggctgagca gacgggcct cgtccgtga aaactacct 
      ctggaaggt ccttttgcg caaaggagc ctccaaaga 
      cggtgtcta ttaccactt aaacggggg tcagtttga 
      ccaggaact gtttcaatt aatttgggg ccccattgt 
      ggccgccct gtcttttgt tttcgacaa atcagagga 
      gcaactgat ttacaaaaa agcaatgac aggattcca 
      gaaatgcgc atttgtttc gtcacactt tgcaatttc 
      gaaaaatct cgacatgtt gatacaaac cctagccaa 
      attcaccaa cttccacaa tttgcaaag accgaacaa 
      cggcattat catgtttgg ggccaatat taaggcgcg 
      tctggcctt gaaatgcgt ccttgactt gtgccacca 
      acacggcga taacattcg agaaggctg gaagaattt 
      attcgattt ggttttgtc gttgtacgt gttgggttt 
      gttgccgag aagttacgt cgaagctga ggattttat 
      cgaaagtag cgggaaatt caatttggt ttatgagga 
      ggtggaaaa tttgcaaaa acaagaaag cgggctttt 
      tagttcgtt gtactccta catggtttc gagcgagaa 
      tttatcaaa agtaccaac tgttgaaga acaacaaca 
      catcgatgt ggcgaaaga gacgataag ggagtgcaa 
      ctttgattt ggttataac cgattcgaa gtttcttca 
      cgacgagtc acttaaggc ccttgttgg ggctttggt 
      cgagctgtc gaggccccc agacgtcca aaagagcct 
      cgggtataa ttacaatga aaacgtcgc cgtgttttt 
      cctcgaact tttgatcaa aattgtgat acaaaaccg 
      ggatcgagt catgacaat atggcagac cgtccgcga 
      ccatatcta caccctcgt catgaactc ctcagtctt 
      cgactacca atttctact cgaacgatc agtcatcgg 
      cctcctgcg cattgcaat tcgtctaat gcgcaacga 
      agacatgag ccccatcgt cctccaatc cctccagat 
      gctcctcct cctcaaatc ctccactct gtgtcgaat 
      ttcgcgcca aatctcgtt cggcctcta cgaactcct 
      taaaacctc cgcccagaa cattcacac cgagacaga 
      ctggacgat tattttcac cctattgga gtgcgtggg 
      ggcgggcgc tgtccctcc caaagcagt cgccgacga 
      ccaccccac caccaaatc cgacggcga aggcgtggc 
      ttccgacga cgacacgcc cgaacgtgg ctacacctc 
      cgactccga actaagcaa aagcccgaa gcaccgccc 
      acaaagccc cgcctgtgg ctggatact ggtgggtaa 
      cgaaggcga gattcaacc agtagtggg gcgtaccct 
      ccctccaag tcagtacag tctcgccct tgaacgtaa 
      tttggggcc gcatgaccc gagcagttt gatcaaatg 
      ttgcgaaag tcttgcgtt tctggtcag ggatgtggc 
      tcatatcac gccctataa tttcgacga ttgcgttca 
      ctgtattag gacgtttgt ggaggccag tctccatgg 
      taataggag acaaaggag gggtaaggg gcggagaca 
      gaggcggaa aggggcgga gcgccgaag tccgacgag 
      cagtccgga cgaggatag tgacgagga ggaggtgcc 
      tagtggcta tcaccaaat ttcgataca attgttaga 
      tttgatgca caccctgca tactagaac cgcccagat 
      tttcaaatg gtgggcgga ggaaggcgg ggctttggc 
      caaagaaac gtcgttgtg gacgcaagg ctggtgccc 
      gttgcttca aggcattgc caggctgtg ttgcgacgt 
      caggagaga gatccgcat gagcgcaat tacatacct 
      gcagcgagc gctcctggt ccacgactt gcagacgct 
      cacgggccc cgaatggga ggcgtgttt ccaccgcgt 
      cctcttccc ccttctggc acacctctt gagtaacat 
      tgaccccaa ggaccccct cgccatgga ggagactcg 
      catgcgcgc cgccaccgt cctctccaa agtgttcct 
      ccaccacct aactccact cctctccct cccaacctt 
      ctccaatct atggctgat tatcctgga cttcattga 
      caagtacat gcacgccga caagagcga tctcctggc 
      ggaagccat cccagaatc gctcaaaaa catgctcct 
      ggtgatgga ctcggcgaa ggtttttga cggaccgga 
      cggcaagag cccgctctg ggccgccac gtgggacag 
      aattaacaa attcctccc agggatgaa ggaggagtt 
      gttcaggga gcagcaggg gaagcggga gaacgtgga 
      ggaggtcgt gcagcacaa tatcgagaa cgcgaccag 
      gtcgatcat cctgcagcc ccccacgag ccaacagca 
      agtctcatc gcccctttt tgcgcattt ggggcagat 
      ggtttcgac gccgattgc ctcggcgct gccgccgcc 
      accgccccc gttcctcca gccgacgct ccagcccgg 
      catgcccta cttatacgc caaaaatca aagtcacac 
      atttccagt gttgtacaa cagtccggt atcgttgta 
      ctccgaata tgtgggaaa cccctacaa taatgccgc 
      tccggaggg ggccacagc tgaggaaaa tacgcagag 
      tttgttaga tgttaataa aaataacga tctagtcga 
      ggctgacgc cccaaataa caacaccaa cgtgtttca 
      gtcttcaaa ttatttcag tgcggactc gggcggcga 
      ctttatacc cccgggctc cgagattct gtttggtac 
      agaacaggg aaacttcat tccagcgtg tacaaatag 
      cgacataaa aacgagcga tgtttaagt gtttctaga 
      ctagaaaat tttaaatcc taattttat gtaaataat 
      tgttcctgt tatttaacg ccctgtccc gcattatat 
      caaagatat atttttact cagttgtta cccaaata
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