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Gene information
General information
TC005825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7152803..7155149
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005825
    iBeetle screen iB_00938

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005825 RA
      MAVVLSQRK WFILLATAT TTTILLMLI NKPNFQAID 
      FNSEQRTTL LLEKITLSN EKPEKNWRG LSEKEISNL 
      SVLGRILYL EEEPPDIPA SKKNVDPFS DCSVKNCQL 
      TYDETALKT ADIVIFHLH RMKPGDLPT RKTSPQSQI 
      WAFLTDESP HHTFLSRNN LTNYNGAFN WSMTYRMDS 
      DIPVPYGRT VLRKTPITD ESILKRRDV LVAIMGSNC 
      GGNNKRWDY VKELQKHIA VDTYGGCGT IKTCPGHFK 
      MDCPQIGEY MFYLAFENS NCDEYVTEK LWWNAFAKN 
      SIPVVMGAS VASYRKLLP PDSYLNVDD YARPKDLAQ 
      YILYLNQTG KYRKYYKWK GDFEVLNEH GYFQSKSYH 
      YCRVCEALN YNQRHKKVY QDLSQFWSA SRDCHPAWN 
      AS
    CDS sequence
    • >TC005825 RA
      atggctgta gtgttatcg cagcgaaaa tggttcatt 
      ttattagcc acggccacg acaacaact attttactt 
      atgttaatc aataaacca aacttccaa gcgatcgat 
      tttaatagc gaacaacgt acgacttta cttttggaa 
      aaaataaca ttaagtaat gaaaaaccg gaaaaaaat 
      tggagagga ttatccgag aaagaaata tcgaatttg 
      tcggtacta ggccgaata ttatatttg gaagaggaa 
      ccacctgac atacccgcc tcgaagaaa aacgtcgat 
      cccttttcc gactgttcg gtaaaaaac tgtcaacta 
      acttacgac gaaacggcg ctaaaaact gccgatatc 
      gtcatattt catttgcac cgaatgaag ccaggggac 
      ctccccaca cgtaaaaca tcaccccag tcccaaatt 
      tgggctttt ttgaccgac gagagcccc catcacacg 
      ttcctatcc cgaaacaac ctcacaaac tacaacggg 
      gccttcaac tggtcgatg acatacagg atggattcg 
      gacatccct gtcccatat gggcgcacg gtcctccgc 
      aaaacgccc atcacggac gaatcgatt ttgaagcgt 
      cgagacgtc ctggtggcc atcatgggc tcgaattgt 
      gggggcaac aacaaacga tgggattac gtgaaagag 
      ttgcaaaaa cacatcgcg gtggacacg tatgggggc 
      tgcgggacg attaaaacg tgtccaggg cacttcaaa 
      atggactgc ccccaaatc ggcgagtat atgttttat 
      ttagcgttc gagaactcc aactgtgac gaatacgtc 
      acggagaag ctttggtgg aacgccttt gcgaaaaac 
      tcgatcccc gttgttatg ggggcttcc gtggcgagt 
      tacaggaaa ctgctgccg cccgattcg tatttaaac 
      gtcgatgac tacgcgcgc cccaaagac ctggcccag 
      tacatcctc tacttgaac caaaccggc aaataccgg 
      aagtattac aagtggaaa ggggatttt gaggtcttg 
      aacgagcac gggtacttc cagtccaag tcttaccac 
      tattgcagg gtctgtgag gccctgaat tacaaccaa 
      aggcacaaa aaagtctac caggacttg agccaattc 
      tggagcgcg agccgggac tgccatccg gcgtggaac 
      gccagttga
    mRNA sequence
    • >TC005825 RA
      aaaggtctt gtgataaac ataaaaaag tgaaaatat 
      gtgaaaata acaatttcg gaaataatt gtaattgtt 
      atagtttta gtctgtgat ggtacaaat ggttcctac 
      aaaatgtcg aaataaggt tttgtaata attatggct 
      gtagtgtta tcgcagcga aaatggttc attttatta 
      gccacggcc acgacaaca actatttta cttatgtta 
      atcaataaa ccaaacttc caagcgatc gattttaat 
      agcgaacaa cgtacgact ttacttttg gaaaaaata 
      acattaagt aatgaaaaa ccggaaaaa aattggaga 
      ggattatcc gagaaagaa atatcgaat ttgtcggta 
      ctaggccga atattatat ttggaagag gaaccacct 
      gacataccc gcctcgaag aaaaacgtc gatcccttt 
      tccgactgt tcggtaaaa aactgtcaa ctaacttac 
      gacgaaacg gcgctaaaa actgccgat atcgtcata 
      tttcatttg caccgaatg aagccaggg gacctcccc 
      acacgtaaa acatcaccc cagtcccaa atttgggct 
      tttttgacc gacgagagc ccccatcac acgttccta 
      tcccgaaac aacctcaca aactacaac ggggccttc 
      aactggtcg atgacatac aggatggat tcggacatc 
      cctgtccca tatgggcgc acggtcctc cgcaaaacg 
      cccatcacg gacgaatcg attttgaag cgtcgagac 
      gtcctggtg gccatcatg ggctcgaat tgtgggggc 
      aacaacaaa cgatgggat tacgtgaaa gagttgcaa 
      aaacacatc gcggtggac acgtatggg ggctgcggg 
      acgattaaa acgtgtcca gggcacttc aaaatggac 
      tgcccccaa atcggcgag tatatgttt tatttagcg 
      ttcgagaac tccaactgt gacgaatac gtcacggag 
      aagctttgg tggaacgcc tttgcgaaa aactcgatc 
      cccgttgtt atgggggct tccgtggcg agttacagg 
      aaactgctg ccgcccgat tcgtattta aacgtcgat 
      gactacgcg cgccccaaa gacctggcc cagtacatc 
      ctctacttg aaccaaacc ggcaaatac cggaagtat 
      tacaagtgg aaaggggat tttgaggtc ttgaacgag 
      cacgggtac ttccagtcc aagtcttac cactattgc 
      agggtctgt gaggccctg aattacaac caaaggcac 
      aaaaaagtc taccaggac ttgagccaa ttctggagc 
      gcgagccgg gactgccat ccggcgtgg aacgccagt 
      tgagtcttt tatttattc cagtccaaa gagttaggt 
      tatgtttgt tttttgtac gtttgagat aagagaaat 
      aaaacttac cactaattg gtgattttt gttatccca 
      acacttaaa cagacggcg aaaacgtgg caaaaacgg 
      tgatagaac acattttat ttgacaaaa acaacacca 
      gctaacact agacacaaa atggcaggg ttattgcac 
      cgttacgtt gctgaccct gatttttta taaacacgt 
      aaagtgagt cattatcgc gatttacgc aaccgtaat 
      cagcgaaaa gcacacaaa ttaaaccca acacgatat 
      taattggac cacaatttg cataaacgc ggaattaat 
      aaattaaca acgtaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY