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Gene information
General information
TC005815 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 237060..245839
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005815
    iBeetle screen iB_00935
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    metathorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    Comment: Four animals were dissected. There were 14 held eggs in 1 case and 11 in the 2nd case! The next 2 animals showed wildtype ovaries!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005815 RA
      MQYSTLSRE RKGSVTSTT HNVANWFSS LKRTKKGKN 
      HKSDATSKS AWDLATLAT YRTTLAPLM CSCVRVCAS 
      YVVYGLFPC CCSCSSGHL KDELVEVVS EMESLDSGE 
      DGKNSNKTK MMSIGRKKF NMDPKKGIE YLIEKGLLQ 
      NTAESVAQF LHKGEGLNK TAIGDYLGE KNDFNEKVL 
      QAFVDLHDF TDLILVQAL RQFLWSFRL PGEAQKIDR 
      MMECFAKRY CDCQGENNI FENSDTCYV LSFAIIMLN 
      TSLHNPSVK DKPTIEQFI NMNRGINQG QDLPRELLV 
      GLYESIKAE PFKIPEDDG NDLMHTFFN PDKEGWLWK 
      QGGRYKSWK RRWFILNDN CLYYFEYTT DKEPRGIIP 
      LENISVREC TDRQKQHCF ELYASGGAD FIKACKTDS 
      EGKVVEGKH TVYRMSASS EEEKNEWMQ RLKQSISHN 
      PFYDMLANR KRKAQHHN
    CDS sequence
    • >TC005815 RA
      atgcagtac tcgactttg agccgtgaa cgtaaaggt 
      tcagtgact agtacaacg cataatgtc gccaattgg 
      ttttcctcg ttgaaacgt acgaaaaaa ggcaaaaac 
      cacaagagc gatgcgacg tcgaaaagc gcctgggat 
      ttggccact ttggccacc tacaggaca acgttggct 
      ccactgatg tgttcgtgt gtgcgtgtg tgtgcctcg 
      tacgttgtt tacggtttg ttcccttgt tgttgctcg 
      tgttcgtca gggcacctc aaagatgaa ttggtggag 
      gtcgtctcc gagatggag tcgctcgat tcgggcgaa 
      gacggtaaa aacagcaac aagaccaaa atgatgtcg 
      attggaagg aagaaattt aatatggat ccgaaaaaa 
      gggatcgag tacctgatt gagaaagga ttattgcag 
      aatacggcc gaaagtgtg gcgcaattt ttgcacaaa 
      ggcgaggga ctgaataag acagcgatc ggtgattat 
      ttgggagag aaaaacgat tttaacgaa aaggtgctg 
      caagctttc gtcgatctg cacgatttt accgatctc 
      atactagtc caggcattg aggcaattc ctttggagc 
      ttccgtttg cccggggaa gcccagaaa atcgaccga 
      atgatggaa tgtttcgcg aagcgctac tgcgactgc 
      cagggcgag aacaacatt tttgaaaat tcggacact 
      tgttacgtg ttatcgttc gcgataata atgcttaac 
      acgagtttg cataatccg agtgtgaaa gataagccc 
      acgatcgag cagtttatt aacatgaac cgggggatt 
      aaccagggt caagacctg cctcgagaa ttacttgtg 
      ggtttgtat gagagtatt aaggccgaa ccgtttaaa 
      atccctgag gatgatggg aatgatttg atgcacacg 
      ttcttcaat ccggataag gaggggtgg ctgtggaag 
      caagggggg cggtacaag agttggaag aggcggtgg 
      tttattttg aacgataat tgtttgtat tacttcgag 
      tatacgact gacaaggaa ccgcgcggg attattccg 
      ttggagaat atttcagtg agggagtgt acagatagg 
      cagaagcag cactgtttc gagttgtat gcgagtggg 
      ggcgctgat ttcatcaag gcttgtaag acggactcg 
      gaggggaaa gttgttgaa gggaaacac actgtatac 
      aggatgtcg gcgagcagt gaagaagag aagaacgaa 
      tggatgcaa aggttgaag cagagtatt agtcataat 
      ccgttctat gatatgttg gcaaacagg aaacggaaa 
      gctcaacat cataactaa
    mRNA sequence
    • >TC005815 RA
      taagcgcgg cgcaaggat cggagtgtg tctgtgggt 
      gataagagg atgtttgtg ccttaggag ttgtgatat 
      tgttgtgga aaaatcgaa ttgtcaaat aaattccaa 
      gtgtcaaga tgaaatcgt gtgtgtgtt aagtaaata 
      aatataagt gttgtgtgt tttcttcat tctgatcat 
      tacttgagt gtgtgtttt tatcgaacg tgccatgcg 
      atgcagtac tcgactttg agccgtgaa cgtaaaggt 
      tcagtgact agtacaacg cataatgtc gccaattgg 
      ttttcctcg ttgaaacgt acgaaaaaa ggcaaaaac 
      cacaagagc gatgcgacg tcgaaaagc gcctgggat 
      ttggccact ttggccacc tacaggaca acgttggct 
      ccactgatg tgttcgtgt gtgcgtgtg tgtgcctcg 
      tacgttgtt tacggtttg ttcccttgt tgttgctcg 
      tgttcgtca gggcacctc aaagatgaa ttggtggag 
      gtcgtctcc gagatggag tcgctcgat tcgggcgaa 
      gacggtaaa aacagcaac aagaccaaa atgatgtcg 
      attggaagg aagaaattt aatatggat ccgaaaaaa 
      gggatcgag tacctgatt gagaaagga ttattgcag 
      aatacggcc gaaagtgtg gcgcaattt ttgcacaaa 
      ggcgaggga ctgaataag acagcgatc ggtgattat 
      ttgggagag aaaaacgat tttaacgaa aaggtgctg 
      caagctttc gtcgatctg cacgatttt accgatctc 
      atactagtc caggcattg aggcaattc ctttggagc 
      ttccgtttg cccggggaa gcccagaaa atcgaccga 
      atgatggaa tgtttcgcg aagcgctac tgcgactgc 
      cagggcgag aacaacatt tttgaaaat tcggacact 
      tgttacgtg ttatcgttc gcgataata atgcttaac 
      acgagtttg cataatccg agtgtgaaa gataagccc 
      acgatcgag cagtttatt aacatgaac cgggggatt 
      aaccagggt caagacctg cctcgagaa ttacttgtg 
      ggtttgtat gagagtatt aaggccgaa ccgtttaaa 
      atccctgag gatgatggg aatgatttg atgcacacg 
      ttcttcaat ccggataag gaggggtgg ctgtggaag 
      caagggggg cggtacaag agttggaag aggcggtgg 
      tttattttg aacgataat tgtttgtat tacttcgag 
      tatacgact gacaaggaa ccgcgcggg attattccg 
      ttggagaat atttcagtg agggagtgt acagatagg 
      cagaagcag cactgtttc gagttgtat gcgagtggg 
      ggcgctgat ttcatcaag gcttgtaag acggactcg 
      gaggggaaa gttgttgaa gggaaacac actgtatac 
      aggatgtcg gcgagcagt gaagaagag aagaacgaa 
      tggatgcaa aggttgaag cagagtatt agtcataat 
      ccgttctat gatatgttg gcaaacagg aaacggaaa 
      gctcaacat cataactaa tttttttat acataataa 
      aggtttgat cggaagggt cgtttgatt tctgtgaaa 
      ttgaacgag agtgcgaaa tctccaatt cctagtctt 
      ggttgacat attatttat ttattgtac ataatgttt 
      tcgttgcaa cggatgtat atacaggtg tattttgtt 
      ttttgtata gattttgtg taaataaat ttacaagtt 
      tcta