Status
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Gene information
General information
TC005814 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7312280..7316179
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005814
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00934

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005814 RA
      MTMESNSHK FKQKKSLSR YLLPNKIPN KVGVAVNRS 
      QSAREPRTE HEEGPPKRS MTISKRPLS LNVANMSPT 
      KDPSNFLQI NSPLDRGTS VSENNVADA SLTDDNVLM 
      EKSISHESV MKEAQVLYL IPTMQARQR NFLQGKIGA 
      NSLLGPREL ERVFPNREV TIFVGTWNM NGHTPPKEL 
      NDFVLPIGI KHVPDILVF GTQESCSER FEWEVCLQE 
      TLGPSHILY HSISLGTLH LAVFVRRDL IWYISIPED 
      ASLSVRPGT AFRTKGAVA CSFLLFGTS FLFVTAHLT 
      AHQEKVKER VTDVRKIVN SLDLPKVLP CKNKSKDVT 
      QNFDYVFWS GDLNFRLAT PRSKVLEWL SKTSFPLPE 
      HLPHGYLHH DQLCSVLAD GAAFKGFTE AKITFPPTY 
      KYDPGTQNF DTSSKQRTP AYTDRILFK HRAGRRFSG 
      QMETPPLQC LVYDSVSSI TTSDHKPVW GVFKAHLRP 
      GLDTIPLAA GLFHRDVYL EGLKRRATL NDSKGATAV 
      CCIQ
    CDS sequence
    • >TC005814 RA
      atgactatg gaaagcaat tctcacaaa ttcaaacag 
      aaaaagtcg ctctcccgg tacctcctc cccaacaaa 
      atcccaaac aaagtcggt gttgcagtg aaccgtagt 
      caaagtgcc cgcgagccg agaaccgag catgaagag 
      ggccccccg aagcgctcg atgacgata tccaagcgc 
      cccttgagt ctcaacgtc gccaacatg agccccacc 
      aaggacccc tcgaatttc ctgcaaatc aactcgccc 
      ctggaccgg ggcacaagc gtctcggag aacaacgta 
      gccgacgcg agtctcacc gacgacaac gttttaatg 
      gagaaaagc atctcgcat gaaagcgtc atgaaagaa 
      gcacaggtt ttgtatttg attccgacg atgcaagcc 
      cgccagcgc aatttcctg caagggaaa atcggggcc 
      aactccctg ctgggcccc cgggagctg gagcgggtg 
      ttccccaat cgcgaagtc acgattttt gtcggcacc 
      tggaacatg aacgggcat acgcccccc aaggaattg 
      aacgacttt gtgcttccc attggcatc aaacacgta 
      cctgatatt ctagttttc gggactcag gagtcttgt 
      tccgagagg tttgaatgg gaggtttgc ctccaggag 
      actctcgga ccctcgcat atcttgtat cattccata 
      tcattaggc actctccac ttggcggtt ttcgtccgc 
      cgcgacctc atctggtac atatcgatc cccgaagac 
      gcctctctg tccgtccgg ccagggacg gcgttccgc 
      accaagggg gcggtggct tgctcgttc ctcctcttc 
      ggcacctcc ttcctcttc gtcaccgcc cacttgacc 
      gcccaccag gagaaggtg aaagagcgc gtcaccgac 
      gtgcgcaaa attgtcaat tcgctcgac ttacccaaa 
      gtcctgccc tgtaaaaac aaaagcaag gacgtcacc 
      caaaatttt gattacgtt ttctggtcc ggagacttg 
      aatttccgg ttggccacg ccccggagt aaggttctg 
      gagtggttg tcgaagaca agtttcccg ctaccggag 
      catctgccc cacgggtat ttacaccac gatcagttg 
      tgttcggtt ttggcggat ggggcggcg tttaaggga 
      tttaccgaa gcgaaaatc acttttcct ccgacttat 
      aaatacgac cctgggacg caaaatttt gacacttct 
      tccaagcag agaacgccg gcgtatacc gatcggatt 
      ttgtttaag catagggcg gggcgaagg tttagtgga 
      cagatggag actccgccc ctccagtgt ctggtctat 
      gattcggtt tcttccatc acgacgtcg gatcataag 
      ccggtgtgg ggcgtgttt aaggcgcac ttaaggccg 
      ggcctggat acgataccg ttagccgcc ggtttgttc 
      catagagat gtttatttg gaaggtttg aaacgtcga 
      gcgacttta aatgattcc aaaggcgcc acagctgtg 
      tgttgtata caataa
    mRNA sequence
    • >TC005814 RA
      tctaggtta tgtagtttg aaaaaattt ttattttat 
      tttcgtaca aaaacgcga taattgcgt cgtttttcg 
      ttgcgtttc gaatcggtt taccatgtg tgaggtacc 
      ggggctcca atgcgggtt catttcacg tgataaaag 
      caataattc gttcgtaag tgccaatac aaacaaatc 
      ctgaatttc gcaaagcaa tgactatgg aaagcaatt 
      ctcacaaat tcaaacaga aaaagtcgc tctcccggt 
      acctcctcc ccaacaaaa tcccaaaca aagtcggtg 
      ttgcagtga accgtagtc aaagtgccc gcgagccga 
      gaaccgagc atgaagagg gccccccga agcgctcga 
      tgacgatat ccaagcgcc ccttgagtc tcaacgtcg 
      ccaacatga gccccacca aggacccct cgaatttcc 
      tgcaaatca actcgcccc tggaccggg gcacaagcg 
      tctcggaga acaacgtag ccgacgcga gtctcaccg 
      acgacaacg ttttaatgg agaaaagca tctcgcatg 
      aaagcgtca tgaaagaag cacaggttt tgtatttga 
      ttccgacga tgcaagccc gccagcgca atttcctgc 
      aagggaaaa tcggggcca actccctgc tgggccccc 
      gggagctgg agcgggtgt tccccaatc gcgaagtca 
      cgatttttg tcggcacct ggaacatga acgggcata 
      cgcccccca aggaattga acgactttg tgcttccca 
      ttggcatca aacacgtac ctgatattc tagttttcg 
      ggactcagg agtcttgtt ccgagaggt ttgaatggg 
      aggtttgcc tccaggaga ctctcggac cctcgcata 
      tcttgtatc attccatat cattaggca ctctccact 
      tggcggttt tcgtccgcc gcgacctca tctggtaca 
      tatcgatcc ccgaagacg cctctctgt ccgtccggc 
      cagggacgg cgttccgca ccaaggggg cggtggctt 
      gctcgttcc tcctcttcg gcacctcct tcctcttcg 
      tcaccgccc acttgaccg cccaccagg agaaggtga 
      aagagcgcg tcaccgacg tgcgcaaaa ttgtcaatt 
      cgctcgact tacccaaag tcctgccct gtaaaaaca 
      aaagcaagg acgtcaccc aaaattttg attacgttt 
      tctggtccg gagacttga atttccggt tggccacgc 
      cccggagta aggttctgg agtggttgt cgaagacaa 
      gtttcccgc taccggagc atctgcccc acgggtatt 
      tacaccacg atcagttgt gttcggttt tggcggatg 
      gggcggcgt ttaagggat ttaccgaag cgaaaatca 
      cttttcctc cgacttata aatacgacc ctgggacgc 
      aaaattttg acacttctt ccaagcaga gaacgccgg 
      cgtataccg atcggattt tgtttaagc atagggcgg 
      ggcgaaggt ttagtggac agatggaga ctccgcccc 
      tccagtgtc tggtctatg attcggttt cttccatca 
      cgacgtcgg atcataagc cggtgtggg gcgtgttta 
      aggcgcact taaggccgg gcctggata cgataccgt 
      tagccgccg gtttgttcc atagagatg tttatttgg 
      aaggtttga aacgtcgag cgactttaa atgattcca 
      aaggcgcca cagctgtgt gttgtatac aataaatga 
      ccagtccta gtctaagtg tcacacatg ccttctact 
      aagtgtacc tatctactg ttctagtca gcaatatta 
      gcacaaggt tttttggtt atgttctag tcaattttt 
      agtcattgt tgattttta tttatttat tgttaaaaa 
      tagagtaga ttttatgta ccctttatg ctacagggt 
      gaccaaata ttttcaaaa aaatttggt acaaactac 
      gccttttca aactcagtg atattctta tatcagact 
      aaattttcg aaagtattt tttcattcc gtctccgga 
      tcacttcat tgtgattct tgttactat ttgttggaa 
      atcctaata aatctattc actgaaa
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