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Gene information
General information
TC005801 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7446379..7448334
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005801
    iBeetle screen iB_00932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature latefluorescence broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: meaning they died after hatching
    larval musculature random rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005801 RA
      MINTGKLQG LTVFITGAS RGIGKAIAL KAAKDGANI 
      VVAAKTAEP HPKLPGTIY TACKEIEEA GGKGLPCVV 
      DVRDEKQVR NAVEEAVKK FGGIDIVIN NASAISLTP 
      TPETDMKRY DLMHNINTR GTFLVSKEC LPYLKKSKH 
      AHILNLSPP LNMKPHWFQ NHVAYTMAK YGMSMCVLG 
      MHEEFRPFN IGVNALWPR TGIHTAAIE MLTGPDSAK 
      FNRKPEIVA DAAYAVLIS DPKTTTGNF FIDDEVLKK 
      HGVTDFDQY CVDPKYKDK LMPDFFVDD DAIKAPSDG 
      SPSLATPKI QGDGKIVKI FQGIEANLS PDTVKGTQA 
      VFQFVVTGE EAGKWFVDL KNGSGACGR GDAPTTPDA 
      TLTMDSKNF FDMFSGKIK PATAFMMGK LKIKGNMQK 
      ALKLEKLMS SLKAKL
    CDS sequence
    • >TC005801 RA
      atgataaat acaggtaaa ttacagggg cttacggtc 
      ttcatcact ggggctagt cggggcatt ggcaaagcc 
      atagccctc aaagcggcc aaagatggg gctaacata 
      gtcgtggcg gccaaaacg gccgaacca caccccaaa 
      ttacccggg accatctac acagcatgc aaggaaatc 
      gaggaggct gggggcaaa ggcctgccc tgtgttgtg 
      gacgtccgc gacgaaaag caggtccgt aacgccgtc 
      gaagaggcc gtgaagaaa ttcggaggc attgacatt 
      gtcatcaat aacgcctcc gcgatttct ctaactccg 
      acccctgaa acggacatg aaacgatac gatttgatg 
      cataatatc aacacgagg gggacgttc ctggtttca 
      aaagagtgt ctcccctat ttgaagaaa agcaaacac 
      gcacacatc ttgaacctg tcgccacct ttaaacatg 
      aaaccccat tggttccag aaccacgtg gcctacact 
      atggccaaa tacggcatg tctatgtgt gttctaggc 
      atgcatgag gagtttaga cccttcaat attggggtt 
      aatgcgctg tggccccgg accgggatt cacactgcg 
      gccattgaa atgctaaca gggcctgac tcagccaag 
      ttcaaccgg aaaccggaa attgtggcc gacgccgcc 
      tacgccgta ctaatctcc gaccccaaa accactaca 
      ggaaacttc tttatcgac gacgaagtc ctcaaaaaa 
      cacggagtc accgacttc gaccaatat tgcgtcgac 
      cccaagtat aaagacaaa ctaatgcca gactttttc 
      gtcgacgat gacgccatc aaagcccct agtgacggg 
      agtccctct ttggccacc cccaaaata cagggtgac 
      ggcaaaatc gttaaaatt ttccaaggg attgaggca 
      aacttatca ccagatacc gtcaagggt actcaagcc 
      gtcttccag tttgtggtg actggagaa gaggccgga 
      aaatggttt gtggacctg aaaaacggt tcaggggct 
      tgcggacgt ggcgacgcc cccaccacc cccgacgcc 
      accctcacc atggacagt aaaaatttc ttcgatatg 
      ttcagcggg aaaatcaag ccggccaca gccttcatg 
      atgggaaaa ttgaaaatc aagggaaat atgcagaag 
      gcgttgaaa ttggaaaaa ctaatgagc tcacttaaa 
      gccaagttg taa
    mRNA sequence
    • >TC005801 RA
      taactaaac gtaaaaacc taagtttcc gcttaactt 
      aaccatttt ttctatgcc ttcactagc cttttacac 
      ctatttccc atcactatt aatgttttt aaatacaat 
      taatcgcgt cttttccaa atactgatg tatggtaga 
      taatttgca aacgtcaaa ttgacagtt aagtgcaat 
      gtcaactca aagatgttg aactttgaa ttctaatta 
      atttctcat ttgttgtta cgtttactt tatacattt 
      taatttatt tgtaattaa gcttactca agaataaga 
      ctcacttcg ggaaaaaat gataaatac aggtaaatt 
      acaggggct tacggtctt catcactgg ggctagtcg 
      gggcattgg caaagccat agccctcaa agcggccaa 
      agatggggc taacatagt cgtggcggc caaaacggc 
      cgaaccaca ccccaaatt acccgggac catctacac 
      agcatgcaa ggaaatcga ggaggctgg gggcaaagg 
      cctgccctg tgttgtgga cgtccgcga cgaaaagca 
      ggtccgtaa cgccgtcga agaggccgt gaagaaatt 
      cggaggcat tgacattgt catcaataa cgcctccgc 
      gatttctct aactccgac ccctgaaac ggacatgaa 
      acgatacga tttgatgca taatatcaa cacgagggg 
      gacgttcct ggtttcaaa agagtgtct cccctattt 
      gaagaaaag caaacacgc acacatctt gaacctgtc 
      gccaccttt aaacatgaa accccattg gttccagaa 
      ccacgtggc ctacactat ggccaaata cggcatgtc 
      tatgtgtgt tctaggcat gcatgagga gtttagacc 
      cttcaatat tggggttaa tgcgctgtg gccccggac 
      cgggattca cactgcggc cattgaaat gctaacagg 
      gcctgactc agccaagtt caaccggaa accggaaat 
      tgtggccga cgccgccta cgccgtact aatctccga 
      ccccaaaac cactacagg aaacttctt tatcgacga 
      cgaagtcct caaaaaaca cggagtcac cgacttcga 
      ccaatattg cgtcgaccc caagtataa agacaaact 
      aatgccaga ctttttcgt cgacgatga cgccatcaa 
      agcccctag tgacgggag tccctcttt ggccacccc 
      caaaataca gggtgacgg caaaatcgt taaaatttt 
      ccaagggat tgaggcaaa cttatcacc agataccgt 
      caagggtac tcaagccgt cttccagtt tgtggtgac 
      tggagaaga ggccggaaa atggtttgt ggacctgaa 
      aaacggttc aggggcttg cggacgtgg cgacgcccc 
      caccacccc cgacgccac cctcaccat ggacagtaa 
      aaatttctt cgatatgtt cagcgggaa aatcaagcc 
      ggccacagc cttcatgat gggaaaatt gaaaatcaa 
      gggaaatat gcagaaggc gttgaaatt ggaaaaact 
      aatgagctc acttaaagc caagttgta aaccattgg 
      ttttgtata attgttatt tttttacgg agtgatgaa 
      taaaagtta ttttataat caaatatca ggggtgcca 
      acccgtttt gcacttacc gactgattt atacagagt 
      gacgaataa aaattttca atctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY