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Gene information
General information
TC005794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7520515..7530980
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005794
    iBeetle screen iB_00930

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    adult lethality - (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005794 RA
      MAAPPQALV NRNKKHFLG VPAPLGYVA GVGRGATGF 
      TTRSDIGPA RDANDVSDD RHAPPAAKR TKKKDEEEE 
      KEEEEDLND SNYDEFSGY GGSLFSKDP YDKDDAEAD 
      AIYEAIDKR MDEKRKEYR EKRLREELE KYRQERPKI 
      QQQFSDLKR DLQNVSDDE WRSVPDVGD ARNRKQRNP 
      RAEKFTPLP DSVLARNLG GESNTTIDP SSGLASMVP 
      GVATPGMLT PTGDMDLRK IGQARNTLM NVKLSQVSD 
      SVTGQTVVD PKGYLTDLQ SMIPTYGGD INDIKKARL 
      LLKSVRETN PNHPPAWIA SARLEEVTG KVQAARNLI 
      MKGCEVNPQ SEDLWLEAA RINPQDTAK AVIAQAARH 
      IPTSVRIWI KAADVETET KAKRRVYRK ALEHIPNSV 
      RLWKAAVEL ENPEDARIL LSRAVECCP TAVELWLAL 
      ARLETYENA RKVLNKARE NIPTDKQIW TTAAKLEEA 
      NGNHAMVEK IIERAITSL SSNGVEINR EHWFKEAIE 
      SEKGGHVHC CRAIVKAII GYGVEPEDQ KHTWIEDAE 
      NCVSQGAYE CARTVYNHA LATFPGKKS IWLRAAHLE 
      KNHGTRESL EGLLQRAVA HCPKSEILW LMGAKSKWL 
      AGDVPAARG ILALAFQAN PNSEEIWLA AVKLESENR 
      EYERARRLL AKARGSAPT PRVMMKSAK LEWSLNDLK 
      AAQNLLDEA LKVFPDFAK LWMMMGQIH EQEGELSKA 
      FDVYYGAIK KCPNSIPLW ILLSRLEEK RGLLIKARS 
      MLEKARLKN PKNDQLWLE AIRIEKRAG MKDIANAMM 
      AKALQECPT SGPLWAESI FMEARPQRK TRSVDALKK 
      CEHDPHVLL AVSKLFWSE RKIGKCREW FQRTIKIDS 
      DFGDAWAYW YRFEQLHGT VEQQEDVKQ RCLAAEPHH 
      GELWCSISK DIKNVGLSN EQLLTLVAK EVVIPV
    CDS sequence
    • >TC005794 RA
      atggcagcc ccaccgcag gcgttagtc aaccgaaac 
      aagaagcac ttcctgggg gtccccgcg cccttgggt 
      tacgtcgct ggggttggt cgaggggct acgggcttc 
      accacacgg tctgatatc gggccagcg agggacgcc 
      aacgacgtt tcggatgat aggcatgcc ccaccggcg 
      gcaaaacgc actaaaaag aaagatgaa gaggaggag 
      aaagaggaa gaagaagat ttgaatgat tcgaattat 
      gatgagttt agcggttat ggggggtca cttttttcc 
      aaagatccc tacgataag gatgatgct gaggctgac 
      gcgatttac gaagcgatt gataagaga atggacgag 
      aagcgaaag gagtacagg gagaagagg ttgagggag 
      gaattggag aaatatcga caagaaaga ccaaaaatc 
      cagcaacag ttctccgac ttgaaacgc gacctgcag 
      aacgtctcc gacgacgaa tggcggagc gtcccagac 
      gtgggggac gcccgtaac cggaaacag cgcaacccc 
      cgagccgaa aaattcacc cctttgccc gacagcgtc 
      ctagcccga aacctgggt ggcgaatcc aacaccacc 
      attgacccc agttcgggg ctagcaagc atggttccg 
      ggtgttgcc acccccgga atgctcacc ccgaccgga 
      gacatggac ttgcgaaaa atcggacaa gcccgcaac 
      actctcatg aatgttaag ttgtcgcaa gtgtccgat 
      tcggtgact ggacaaaca gttgtggac ccaaagggg 
      tatttgacc gatttacaa tcaatgatt cccacctac 
      ggtggggat attaatgat attaagaag gcgagattg 
      ttgcttaaa tcggtgagg gagacgaat ccgaatcat 
      cctccggcg tggattgct agcgccagg ctggaggaa 
      gttacgggg aaggtgcaa gcggcgagg aatttgatt 
      atgaaaggg tgtgaggtt aatccacaa agtgaggat 
      ttgtggctg gaggcggcc aggattaat ccccaggat 
      acggccaag gcggtgatt gcgcaggcg gccaggcat 
      attccaacg tcggtacgg atctggatc aaggctgca 
      gatgttgaa accgaaaca aaagctaaa cggcgagtc 
      taccgaaaa gccctagaa cacatcccg aattcggtc 
      cgtctctgg aaagccgcc gtggaactg gaaaatcca 
      gaagacgcc cgaattctc ctatccaga gccgtggag 
      tgttgtccc actgccgtc gaattatgg ttagccctc 
      gcaagactc gaaacgtac gaaaatgcc agaaaagtc 
      ttaaataaa gctcgagaa aatattccc actgataaa 
      caaatctgg acaactgcg gccaagctg gaagaagcc 
      aatggtaat cacgccatg gtggagaaa atcatcgag 
      agggctata acttcatta agctcgaac ggagttgag 
      ataaatcga gagcactgg tttaaagaa gctatagaa 
      agtgaaaaa gggggccac gtgcactgc tgtcgcgcc 
      atcgtcaag gcaatcatc ggatatggg gtggagccc 
      gaggaccag aagcacacc tggatcgag gacgccgaa 
      aattgtgtg agccagggg gcgtacgaa tgcgcccgc 
      actgtctac aaccacgcc cttgccacc ttcccgggc 
      aaaaaatca atttggttg cgtgccgcc catttggag 
      aaaaaccac ggaacgcgg gaaagcctc gaggggctc 
      cttcaacga gccgttgcc cattgcccc aaaagtgag 
      attttgtgg ctcatgggg gcgaaaagc aagtggttg 
      gcgggtgac gtacccgca gcccgaggg atcctcgcc 
      ctggccttt caggctaac cccaacagt gaggaaatt 
      tggcttgct gcagtcaaa cttgagtca gaaaatcgc 
      gaatatgag agggcgagg aggttattg gccaaggcc 
      aggggctcc gcccccacg ccgagggtc atgatgaag 
      agtgccaag ttggagtgg tcgctcaat gacttgaaa 
      gcggcccag aatttgtta gatgaagcc ttgaaggtc 
      ttcccggat ttcgccaag ttgtggatg atgatgggg 
      cagatacat gaacaggag ggcgagttg tcgaaggct 
      ttcgatgtg tattatggg gcgatcaaa aaatgcccg 
      aattccatc ccgttgtgg attttgctg tcgcgcctc 
      gaagaaaag cgcggtttg ttgataaaa gcgcgctca 
      atgttggaa aaagcccgc ttgaaaaac cccaaaaac 
      gaccaactg tggcttgag gcaatccgt attgaaaaa 
      cgggccggt atgaaagac atcgcgaac gcaatgatg 
      gccaaggcg cttcaagag tgccccact tcggggccc 
      ctctgggcc gagtcgatt ttcatggaa gcgcggcct 
      caacgaaag acacgttct gtggacgcg ctcaagaag 
      tgcgagcac gacccacat gtcctccta gccgtcagc 
      aagttattc tggtcggag cgtaaaatc gggaaatgc 
      cgggaatgg ttccaaagg actattaaa atcgattcg 
      gattttggg gacgcgtgg gcttattgg tacaggttt 
      gagcagctt cacggaacg gtggagcaa caggaggat 
      gtgaaacag aggtgtttg gcggcggag ccccatcat 
      ggggagttg tggtgctcc atttccaag gacattaag 
      aatgtgggg ctgagtaat gagcagctt ttgaccctg 
      gttgcgaaa gaagttgtt attccagtc tga
    mRNA sequence
    • >TC005794 RA
      ttatggcag ccccaccgc aggcgttag tcaaccgaa 
      acaagaagc acttcctgg gggtccccg cgcccttgg 
      gttacgtcg ctggggttg gtcgagggg ctacgggct 
      tcaccacac ggtctgata tcgggccag cgagggacg 
      ccaacgacg tttcggatg ataggcatg ccccaccgg 
      cggcaaaac gcactaaaa agaaagatg aagaggagg 
      agaaagagg aagaagaag atttgaatg attcgaatt 
      atgatgagt ttagcggtt atggggggt cactttttt 
      ccaaagatc cctacgata aggatgatg ctgaggctg 
      acgcgattt acgaagcga ttgataaga gaatggacg 
      agaagcgaa aggagtaca gggagaaga ggttgaggg 
      aggaattgg agaaatatc gacaagaaa gaccaaaaa 
      tccagcaac agttctccg acttgaaac gcgacctgc 
      agaacgtct ccgacgacg aatggcgga gcgtcccag 
      acgtggggg acgcccgta accggaaac agcgcaacc 
      cccgagccg aaaaattca cccctttgc ccgacagcg 
      tcctagccc gaaacctgg gtggcgaat ccaacacca 
      ccattgacc ccagttcgg ggctagcaa gcatggttc 
      cgggtgttg ccacccccg gaatgctca ccccgaccg 
      gagacatgg acttgcgaa aaatcggac aagcccgca 
      acactctca tgaatgtta agttgtcgc aagtgtccg 
      attcggtga ctggacaaa cagttgtgg acccaaagg 
      ggtatttga ccgatttac aatcaatga ttcccacct 
      acggtgggg atattaatg atattaaga aggcgagat 
      tgttgctta aatcggtga gggagacga atccgaatc 
      atcctccgg cgtggattg ctagcgcca ggctggagg 
      aagttacgg ggaaggtgc aagcggcga ggaatttga 
      ttatgaaag ggtgtgagg ttaatccac aaagtgagg 
      atttgtggc tggaggcgg ccaggatta atccccagg 
      atacggcca aggcggtga ttgcgcagg cggccaggc 
      atattccaa cgtcggtac ggatctgga tcaaggctg 
      cagatgttg aaaccgaaa caaaagcta aacggcgag 
      tctaccgaa aagccctag aacacatcc cgaattcgg 
      tccgtctct ggaaagccg ccgtggaac tggaaaatc 
      cagaagacg cccgaattc tcctatcca gagccgtgg 
      agtgttgtc ccactgccg tcgaattat ggttagccc 
      tcgcaagac tcgaaacgt acgaaaatg ccagaaaag 
      tcttaaata aagctcgag aaaatattc ccactgata 
      aacaaatct ggacaactg cggccaagc tggaagaag 
      ccaatggta atcacgcca tggtggaga aaatcatcg 
      agagggcta taacttcat taagctcga acggagttg 
      agataaatc gagagcact ggtttaaag aagctatag 
      aaagtgaaa aagggggcc acgtgcact gctgtcgcg 
      ccatcgtca aggcaatca tcggatatg gggtggagc 
      ccgaggacc agaagcaca cctggatcg aggacgccg 
      aaaattgtg tgagccagg gggcgtacg aatgcgccc 
      gcactgtct acaaccacg cccttgcca ccttcccgg 
      gcaaaaaat caatttggt tgcgtgccg cccatttgg 
      agaaaaacc acggaacgc gggaaagcc tcgaggggc 
      tccttcaac gagccgttg cccattgcc ccaaaagtg 
      agattttgt ggctcatgg gggcgaaaa gcaagtggt 
      tggcgggtg acgtacccg cagcccgag ggatcctcg 
      ccctggcct ttcaggcta accccaaca gtgaggaaa 
      tttggcttg ctgcagtca aacttgagt cagaaaatc 
      gcgaatatg agagggcga ggaggttat tggccaagg 
      ccaggggct ccgccccca cgccgaggg tcatgatga 
      agagtgcca agttggagt ggtcgctca atgacttga 
      aagcggccc agaatttgt tagatgaag ccttgaagg 
      tcttcccgg atttcgcca agttgtgga tgatgatgg 
      ggcagatac atgaacagg agggcgagt tgtcgaagg 
      ctttcgatg tgtattatg gggcgatca aaaaatgcc 
      cgaattcca tcccgttgt ggattttgc tgtcgcgcc 
      tcgaagaaa agcgcggtt tgttgataa aagcgcgct 
      caatgttgg aaaaagccc gcttgaaaa accccaaaa 
      acgaccaac tgtggcttg aggcaatcc gtattgaaa 
      aacgggccg gtatgaaag acatcgcga acgcaatga 
      tggccaagg cgcttcaag agtgcccca cttcggggc 
      ccctctggg ccgagtcga ttttcatgg aagcgcggc 
      ctcaacgaa agacacgtt ctgtggacg cgctcaaga 
      agtgcgagc acgacccac atgtcctcc tagccgtca 
      gcaagttat tctggtcgg agcgtaaaa tcgggaaat 
      gccgggaat ggttccaaa ggactatta aaatcgatt 
      cggattttg gggacgcgt gggcttatt ggtacaggt 
      ttgagcagc ttcacggaa cggtggagc aacaggagg 
      atgtgaaac agaggtgtt tggcggcgg agccccatc 
      atggggagt tgtggtgct ccatttcca aggacatta 
      agaatgtgg ggctgagta atgagcagc ttttgaccc 
      tggttgcga aagaagttg ttattccag tctgagtta 
      gtgttactt aatagaaat aagcgagga caggtattt 
      ttttccagt tattttttc cctgttcct tttttcttg 
      tgttggttt gatttgtgc acggtatga ggggctaaa 
      tattacgag atacggtct tgcaactgt aaagtatgt 
      gatgaatta gagttctgc aaaaattaa tggcaaact 
      ttatgcgac ttttatatt tctctttaa atgaaatat 
      caattaaac aacttaaaa catt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY