Status
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Gene information
General information
TC005782 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7755621..7764958
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005782
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00928

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005782 RA
      MLSILSLKI VFLLYLVKG QDNCAETKA ACEDQTDCS 
      SCIREHPCC VFCYDEDFP APRCFKKGK TTTCNQQFL 
      VNNTETKIA SNTIEFRSV SDDVDSAIQ LIPTEFNVT 
      LRVGKPVNL TFRYKPAEN YPLDLYYLM DLTKSMEGT 
      LNKIKTLGQ NLTILLENL TENHQIAFG SFIDKPEMP 
      FYMTNPENY KNPCGIDFI TCEQGYLFK HRLNFTKNI 
      NEFIEKVNG SRLSANNDD YEGGMDALM QILTCGENF 
      QWLEKSRKL VVVATDGFM HLAGDGLLV GHVKKINNE 
      KCLINSEGN YRSKIPYDY PSLEEVYHK LKAEKTNVI 
      VAVATPKAY PYYQQMSQM LNNSFFVGQ LKNDSTNVL 
      NVVKEGYEA FAQLVTFFA NTSEIRQLQ VDFFTDCED 
      NGEWVRGSM CSNARIGKE MTFKAQLML KECPSHDSH 
      TIYVEENNI YEKIAINLN FAGCMCHCS NMRKGAKCG 
      NGFWDCNKC TCEDGWTGA TCTNRCENN HEKCRSATN 
      GQVSLTCSG FGDCVCGKC KCYFPYTGS ICQYKCPTD 
      KNGQICSGH GECVENGQC ECDPQYSGQ DCSCLESQE 
      GCRYSDGIC SENGKCECN SCNCERGYS GRFCQICDQ 
      CKQMCADYD LIVAKGHNV TKGGRRIVL DSVEDIPED 
      ERNCLTRFT RDDGEYCEV VYKIQSVKS DFVRIKTMP 
      PQCYKPVAA SVYVGLIIG ALLVAGLLF ILGWKARNM 
      IQDKREYNE FVKNRQMNQ TNENPLYKS PLVTYKNPM 
      QQVRVHEKT N
    CDS sequence
    • >TC005782 RA
      atgttgtca attttatct ctgaaaatt gtgtttctc 
      ctctatttg gttaagggg caagataat tgcgccgaa 
      acaaaagca gcatgtgaa gaccaaacc gattgttcg 
      agttgtatt cgggagcat ccctgctgt gttttttgt 
      tatgatgag gatttccca gctccgcga tgtttcaaa 
      aaaggcaaa acaacaacc tgcaaccaa cagtttctc 
      gtaaacaac accgaaacc aaaatcgca agcaacaca 
      attgaattc cggagcgtt tccgacgac gttgactcc 
      gccattcag ctcattccc accgaattc aatgtgact 
      ttgcgcgtc ggaaaaccg gttaactta acattccgc 
      tacaaacca gccgagaac tatcctctc gacctgtac 
      tatctgatg gacctcacg aaaagcatg gaaggcact 
      ttgaacaaa atcaaaaca cttggccag aacttgacc 
      atacttttg gaaaacctg accgaaaac caccaaatt 
      gcctttggt tccttcatc gacaagccg gaaatgccc 
      ttctacatg acgaacccc gagaactac aaaaacccc 
      tgcgggatt gattttatc acgtgtgag cagggctat 
      ctcttcaaa cacaggcta aattttacg aaaaacatc 
      aacgagttc attgagaaa gtgaacgga agccggctt 
      tctgcaaac aatgacgat tacgagggc ggtatggac 
      gccctcatg caaatttta acatgtggt gagaatttc 
      cagtggttg gaaaagtcc cggaaattg gttgtggtg 
      gcaacggac ggttttatg cacctggca ggtgacggg 
      cttcttgtt ggacacgtc aaaaaaatc aacaacgag 
      aaatgttta ataaactcg gagggaaat taccgttcg 
      aaaatcccc tacgattac ccttccttg gaggaggtt 
      taccacaag ttgaaagcg gaaaaaacc aacgttatc 
      gtcgcagtg gcgacccca aaagcctac ccctactac 
      cagcaaatg tcccaaatg ctcaataac tcgtttttc 
      gtggggcag ttgaaaaac gactcaacc aatgtccta 
      aatgttgtc aaagaaggg tacgaggct tttgcccaa 
      cttgtcacc ttttttgcc aacacttcc gaaatcagg 
      caattgcaa gtcgatttt tttaccgac tgtgaggat 
      aatggtgag tgggtgagg ggatcgatg tgctcaaat 
      gccaggatt gggaaggag atgactttc aaggcgcaa 
      ttgatgctg aaggagtgt cccagtcat gattcccat 
      accatttat gtggaggag aacaacatt tacgagaaa 
      attgccatt aatttgaat ttcgcgggg tgtatgtgt 
      cattgttcg aatatgagg aaaggagcg aagtgtgga 
      aatgggttt tgggactgt aacaagtgt acttgtgag 
      gatgggtgg accggagcg acttgcacc aaccgttgc 
      gaaaacaac cacgagaag tgccgttcg gccaccaac 
      ggccaggtt tccttgact tgttccggc tttggcgac 
      tgtgtttgt ggcaaatgc aaatgctac ttcccctac 
      accggttcc atatgccag tacaagtgc cccaccgac 
      aaaaacggg caaatttgc agcggccat ggcgagtgt 
      gtggagaac gggcaatgc gagtgcgac ccccaatac 
      agcggccag gactgcagc tgcttggag tcgcaagaa 
      gggtgccgc tattccgat ggcatttgc agcgagaat 
      ggaaaatgc gagtgcaac agttgcaac tgtgagcga 
      ggctattct gggcgtttt tgccaaatt tgcgaccag 
      tgcaagcaa atgtgcgct gattacgac ttgattgtg 
      gccaaaggg cacaatgtg acgaaaggc gggcgaagg 
      attgtgctt gactcagtt gaggatatt ccagaggat 
      gaacgcaac tgtctgacg aggtttaca cgggatgat 
      ggggaatat tgtgaagtt gtttacaaa atacaaagc 
      gtgaagagt gattttgtg aggattaaa acaatgcca 
      cctcagtgt tacaaacca gtggctgca agcgtctac 
      gttggactt attataggc gctttatta gtagcaggg 
      ttgcttttt attctgggc tggaaggcc aggaatatg 
      atacaggac aagagggag tacaacgag tttgtcaaa 
      aaccgacaa atgaatcaa acgaacgag aatcctctc 
      tacaagtct ccgcttgtc acgtacaag aatccaatg 
      caacaagtt agggttcac gagaaaact aactag
    mRNA sequence
    • >TC005782 RA
      gttcgatac gtgagaatg ttgtcaatt ttatctctg 
      aaaattgtg tttctcctc tatttggtt aaggggcaa 
      gataattgc gccgaaaca aaagcagca tgtgaagac 
      caaaccgat tgttcgagt tgtattcgg gagcatccc 
      tgctgtgtt ttttgttat gatgaggat ttcccagct 
      ccgcgatgt ttcaaaaaa ggcaaaaca acaacctgc 
      aaccaacag tttctcgta aacaacacc gaaaccaaa 
      atcgcaagc aacacaatt gaattccgg agcgtttcc 
      gacgacgtt gactccgcc attcagctc attcccacc 
      gaattcaat gtgactttg cgcgtcgga aaaccggtt 
      aacttaaca ttccgctac aaaccagcc gagaactat 
      cctctcgac ctgtactat ctgatggac ctcacgaaa 
      agcatggaa ggcactttg aacaaaatc aaaacactt 
      ggccagaac ttgaccata cttttggaa aacctgacc 
      gaaaaccac caaattgcc tttggttcc ttcatcgac 
      aagccggaa atgcccttc tacatgacg aaccccgag 
      aactacaaa aacccctgc gggattgat tttatcacg 
      tgtgagcag ggctatctc ttcaaacac aggctaaat 
      tttacgaaa aacatcaac gagttcatt gagaaagtg 
      aacggaagc cggctttct gcaaacaat gacgattac 
      gagggcggt atggacgcc ctcatgcaa attttaaca 
      tgtggtgag aatttccag tggttggaa aagtcccgg 
      aaattggtt gtggtggca acggacggt tttatgcac 
      ctggcaggt gacgggctt cttgttgga cacgtcaaa 
      aaaatcaac aacgagaaa tgtttaata aactcggag 
      ggaaattac cgttcgaaa atcccctac gattaccct 
      tccttggag gaggtttac cacaagttg aaagcggaa 
      aaaaccaac gttatcgtc gcagtggcg accccaaaa 
      gcctacccc tactaccag caaatgtcc caaatgctc 
      aataactcg tttttcgtg gggcagttg aaaaacgac 
      tcaaccaat gtcctaaat gttgtcaaa gaagggtac 
      gaggctttt gcccaactt gtcaccttt tttgccaac 
      acttccgaa atcaggcaa ttgcaagtc gattttttt 
      accgactgt gaggataat ggtgagtgg gtgagggga 
      tcgatgtgc tcaaatgcc aggattggg aaggagatg 
      actttcaag gcgcaattg atgctgaag gagtgtccc 
      agtcatgat tcccatacc atttatgtg gaggagaac 
      aacatttac gagaaaatt gccattaat ttgaatttc 
      gcggggtgt atgtgtcat tgttcgaat atgaggaaa 
      ggagcgaag tgtggaaat gggttttgg gactgtaac 
      aagtgtact tgtgaggat gggtggacc ggagcgact 
      tgcaccaac cgttgcgaa aacaaccac gagaagtgc 
      cgttcggcc accaacggc caggtttcc ttgacttgt 
      tccggcttt ggcgactgt gtttgtggc aaatgcaaa 
      tgctacttc ccctacacc ggttccata tgccagtac 
      aagtgcccc accgacaaa aacgggcaa atttgcagc 
      ggccatggc gagtgtgtg gagaacggg caatgcgag 
      tgcgacccc caatacagc ggccaggac tgcagctgc 
      ttggagtcg caagaaggg tgccgctat tccgatggc 
      atttgcagc gagaatgga aaatgcgag tgcaacagt 
      tgcaactgt gagcgaggc tattctggg cgtttttgc 
      caaatttgc gaccagtgc aagcaaatg tgcgctgat 
      tacgacttg attgtggcc aaagggcac aatgtgacg 
      aaaggcggg cgaaggatt gtgcttgac tcagttgag 
      gatattcca gaggatgaa cgcaactgt ctgacgagg 
      tttacacgg gatgatggg gaatattgt gaagttgtt 
      tacaaaata caaagcgtg aagagtgat tttgtgagg 
      attaaaaca atgccacct cagtgttac aaaccagtg 
      gctgcaagc gtctacgtt ggacttatt ataggcgct 
      ttattagta gcagggttg ctttttatt ctgggctgg 
      aaggccagg aatatgata caggacaag agggagtac 
      aacgagttt gtcaaaaac cgacaaatg aatcaaacg 
      aacgagaat cctctctac aagtctccg cttgtcacg 
      tacaagaat ccaatgcaa caagttagg gttcacgag 
      aaaactaac taggggact tttttaaca gaaatgttc 
      agcgtattt atttaattg tggtagtgt agctgccga 
      tt
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