| General information | |||
| TC034053
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 7860255..7874635 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034053 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00926 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 3
| Comment: |
- >TC034053 RA
MALTTCLKF FPRAQKRIH GEDVFGNKI KAVTPTLRS YTGRKVKDG AFQGARGQD KRFHEQEFG VRPMAQASS LMDVSYSTR RPRTPQETT LQQVLPNRV RRASGSSEY SDDGNKMLT DCSKPVDLI ISNLDPALN VKELRRLLT NMLKEYATI LSLKVVMQT DGVPVASIR VNNQQAAQF IISQLHRQK LGHKRIMVS YGQSNSPDP EELRAMVIS ILQEAPDNN MPLFKFMEL LESRFHCTV SVSEVNKLK DIIKISDNL GSRIISFTQ QMKIFPPPN LSKTLTPYC TIHCAGGLP NLGWCELNT NQSPNIMMS LRQFAAALD ELLTLHMDS MPLLSFQVC YEHVCHQPL PVDASGVPL EHLISCVPN VEIKNSGPN KNIKVIKRE LKGEVDEET VLKAVPPSL APNIILLCR EVVDLLKTT DRCQLLLSK FIPAYHHHF GRQCHVADY GYIKLIDLF ESIPHVVQI IGDGTRKII TLSHQAQMR RFTSDLLRV LKVQPAKQI TVGDFPAAY ERVMNRVFN PVDYGLCTF QDLLEEVPD NVVVITHND DNLVISVPK REQTAKEIM KTKQFAMEV IDILRHSPN CTMLFTKFV PAYHHHFGH QCRVSDYGF TKLIELFEA IPDVVKIQE LVEGERTIS LTLIQSLKI LGAQIVSFV KNSPTSSIL LDELPGIYL KECGYPLKP QVYESETLL ELFEKLSDY VQVVDTSSG RLVVAVDAN FLAVAKIRV WAILLKAPY RKEFSNFKY EYQSRYNSD IGINDLKQL RTIVTLSVD NDGIYIALT DLYVIAAQL YHLLYTNGG SILFTNLPR LYYQAFGKN VRLSEYKIN TIDQLYEQF DFLFFICGY KKSVLALNR NLAEYQVPV PSQICNWNH QTKKSAPPP ITKSSGAVR KYSPPKPDT PPTPNLAIE LPIIQNPQP MGNPYDLIS PAKLLFSTN QNPWGKAPD PAELPMPDK LMMKGVSDD STDSGVNSK LENSPSDNE TESGACGSG AKRRFPNYL NFDK
- >TC034053 RA
atggctctg accacgtgt ttgaaattt ttccctagg gcacagaaa cgaattcat ggagaggat gtcttcggg aacaagatc aaagctgtt acgccgacc ttgcggagt tacaccggg cggaaggtt aaggatggg gcgttccaa ggggctcgg gggcaagac aagaggttt cacgagcaa gaattcggc gtccgtcca atggcccaa gcatcgtcc cttatggac gtgagttac tcaactcgt cgtcctcgg acacctcag gaaaccacc ctccagcag gtgctgcca aatcgtgtt cgtcgcgca agcggaagt tccgaatac agcgacgat ggcaataaa atgctaact gattgttcc aaacccgtg gatcttatt atttcaaac ctggatcct gcactaaat gttaaagaa ttgcgaaga ttgctaaca aatatgttg aaggaatat gccacgatt ttgagtttg aaagtagtg atgcaaact gatggcgtt cccgtggct agcatcaga gtgaataat caacaggcg gctcagttt attatttcg cagcttcat agacaaaag ttggggcat aagaggatc atggtgtct tatggacag agtaattcc ccggatcca gaggagctg agggccatg gttataagc atattacag gaagccccc gacaacaac atgcccctt ttcaaattc atggaactg ctagaatcg cgtttccat tgcaccgtt tccgtttcg gaagtgaac aaactgaaa gacattatc aaaatcagc gacaatctc ggctctcgc atcatttca ttcacccaa caaatgaaa attttcccg ccaccgaac ttgagcaag actctaaca ccgtactgt acaatccac tgcgccggt ggtttaccc aatctcggt tggtgcgaa ctgaacacc aaccaaagc cccaacatc atgatgtct ttacgccag ttcgccgcc gccctcgac gaactcctc accctgcac atggattcc atgccatta ctgagtttc caggtctgt tacgagcac gtgtgccat cagccgctt ccggtggac gcctccggc gtgcctctg gagcacttg atctcgtgc gtgcccaac gtcgagatt aaaaactcc ggtccgaat aaaaacatc aaagttatt aagcgcgaa ttgaagggc gaagtcgac gaggagacg gtgttgaaa gctgtgccc ccgtctttg gcccccaac atcatcctg ctgtgtcgg gaggtggtc gatttgctc aaaaccacc gatagatgt cagctgctc ctcagtaaa tttatccca gcctaccac caccatttc gggcgtcag tgccacgtt gctgattac ggttacatc aaattaatt gatttattc gaatcaatt ccacatgtt gtgcaaata atcggcgac gggacgcga aaaatcatc acgctttcg catcaagcg caaatgcgc cgttttacg tcggatctg ttgcgagtg ttgaaagtc cagccggca aagcagatc actgtgggc gatttcccg gccgcctat gaacgggtc atgaaccgg gtgttcaat cccgttgat tatggatta tgcacgttt caagattta ctggaagag gtgcccgat aatgttgtg gtgattacg cataatgat gataatttg gtgatttcg gtgccgaaa cgcgaacaa accgccaaa gaaattatg aaaactaag caatttgca atggaggtt attgatatt ttgagacat tcccccaat tgtacgatg ctttttacc aagtttgtg ccggcgtat catcatcat tttggacat cagtgtagg gtctcggat tatgggttt accaagttg atcgagttg tttgaggcg atccctgat gtggtcaag attcaagag ttagtcgag ggtgaacgt accataagc cttacgttg atccaatcg ctcaagatt ttgggcgcg caaatagtg agttttgtg aagaattca ccgacctcg agtatttta ctcgacgaa cttccgggg atttacctg aaagagtgc ggctatccg ctcaagccg caagtgtac gaatcggag acgttgttg gagttgttt gagaaactc agtgattac gtccaggtt gttgatacg agttctggc cgtttggtg gtggccgtc gatgcgaat ttcctcgcc gttgccaaa attcgggtc tgggccatt ttactgaag gccccatat cgtaaagaa ttttccaat ttcaaatac gaatatcaa tcaaggtac aatagcgat attggaatc aacgatttg aaacagttg cgcactatt gtgacgtta tcggtggat aacgatgga atctacatc gctttaacc gatttatac gtcattgcg gcccaactg taccatcta ctgtacacc aatggtggg tccattttg ttcactaat ttaccaaga ctgtactat caagcattt ggcaaaaat gttcgactt tcggagtat aaaatcaac acgatcgat caattgtac gaacagttc gattttttg ttttttatc tgtggttac aagaagagt gtgcttgca ctgaaccgg aatttggcc gaatatcag gtacccgta ccttcgcaa atttgcaat tggaatcat cagacgaag aaatcggcg ccacctccg ataactaaa tcgtcaggg gcggttcgg aaatattct ccgcccaaa cccgacact ccgcccact cctaatttg gcaattgaa ttgcccata atccaaaat ccgcaaccg atgggtaat ccgtatgac ttgatttcg ccagcgaag ctcctgttt tcaacaaac caaaatccg tggggcaaa gcccccgat ccggccgaa ctgccaatg cctgataaa ctaatgatg aaaggtgtg tctgatgat tcgactgat tctggtgtt aattcaaaa ttggaaaat tcaccgtct gataacgaa actgaatca ggggcttgc gggtcaggc gccaaacgt cgtttcccc aattatttg aacttcgat aagtaa
- >TC034053 RA
atgttaatg gctctgacc acgtgtttg aaatttttc cctagggca cagaaacga attcatgga gaggatgtc ttcgggaac aagatcaaa gctgttacg ccgaccttg cggagttac accgggcgg aaggttaag gatggggcg ttccaaggg gctcggggg caagacaag aggtttcac gagcaagaa ttcggcgtc cgtccaatg gcccaagca tcgtccctt atggacgtg agttactca actcgtcgt cctcggaca cctcaggaa accaccctc cagcaggtg ctgccaaat cgtgttcgt cgcgcaagc ggaagttcc gaatacagc gacgatggc aataaaatg ctaactgat tgttccaaa cccgtggat cttattatt tcaaacctg gatcctgca ctaaatgtt aaagaattg cgaagattg ctaacaaat atgttgaag gaatatgcc acgattttg agtttgaaa gtagtgatg caaactgat ggcgttccc gtggctagc atcagagtg aataatcaa caggcggct cagtttatt atttcgcag cttcataga caaaagttg gggcataag aggatcatg gtgtcttat ggacagagt aattccccg gatccagag gagctgagg gccatggtt ataagcata ttacaggaa gcccccgac aacaacatg ccccttttc aaattcatg gaactgcta gaatcgcgt ttccattgc accgtttcc gtttcggaa gtgaacaaa ctgaaagac attatcaaa atcagcgac aatctcggc tctcgcatc atttcattc acccaacaa atgaaaatt ttcccgcca ccgaacttg agcaagact ctaacaccg tactgtaca atccactgc gccggtggt ttacccaat ctcggttgg tgcgaactg aacaccaac caaagcccc aacatcatg atgtcttta cgccagttc gccgccgcc ctcgacgaa ctcctcacc ctgcacatg gattccatg ccattactg agtttccag gtctgttac gagcacgtg tgccatcag ccgcttccg gtggacgcc tccggcgtg cctctggag cacttgatc tcgtgcgtg cccaacgtc gagattaaa aactccggt ccgaataaa aacatcaaa gttattaag cgcgaattg aagggcgaa gtcgacgag gagacggtg ttgaaagct gtgcccccg tctttggcc cccaacatc atcctgctg tgtcgggag gtggtcgat ttgctcaaa accaccgat agatgtcag ctgctcctc agtaaattt atcccagcc taccaccac catttcggg cgtcagtgc cacgttgct gattacggt tacatcaaa ttaattgat ttattcgaa tcaattcca catgttgtg caaataatc ggcgacggg acgcgaaaa atcatcacg ctttcgcat caagcgcaa atgcgccgt tttacgtcg gatctgttg cgagtgttg aaagtccag ccggcaaag cagatcact gtgggcgat ttcccggcc gcctatgaa cgggtcatg aaccgggtg ttcaatccc gttgattat ggattatgc acgtttcaa gatttactg gaagaggtg cccgataat gttgtggtg attacgcat aatgatgat aatttggtg atttcggtg ccgaaacgc gaacaaacc gccaaagaa attatgaaa actaagcaa tttgcaatg gaggttatt gatattttg agacattcc cccaattgt acgatgctt tttaccaag tttgtgccg gcgtatcat catcatttt ggacatcag tgtagggtc tcggattat gggtttacc aagttgatc gagttgttt gaggcgatc cctgatgtg gtcaagatt caagagtta gtcgagggt gaacgtacc ataagcctt acgttgatc caatcgctc aagattttg ggcgcgcaa atagtgagt tttgtgaag aattcaccg acctcgagt attttactc gacgaactt ccggggatt tacctgaaa gagtgcggc tatccgctc aagccgcaa gtgtacgaa tcggagacg ttgttggag ttgtttgag aaactcagt gattacgtc caggttgtt gatacgagt tctggccgt ttggtggtg gccgtcgat gcgaatttc ctcgccgtt gccaaaatt cgggtctgg gccatttta ctgaaggcc ccatatcgt aaagaattt tccaatttc aaatacgaa tatcaatca aggtacaat agcgatatt ggaatcaac gatttgaaa cagttgcgc actattgtg acgttatcg gtggataac gatggaatc tacatcgct ttaaccgat ttatacgtc attgcggcc caactgtac catctactg tacaccaat ggtgggtcc attttgttc actaattta ccaagactg tactatcaa gcatttggc aaaaatgtt cgactttcg gagtataaa atcaacacg atcgatcaa ttgtacgaa cagttcgat tttttgttt tttatctgt ggttacaag aagagtgtg cttgcactg aaccggaat ttggccgaa tatcaggta cccgtacct tcgcaaatt tgcaattgg aatcatcag acgaagaaa tcggcgcca cctccgata actaaatcg tcaggggcg gttcggaaa tattctccg cccaaaccc gacactccg cccactcct aatttggca attgaattg cccataatc caaaatccg caaccgatg ggtaatccg tatgacttg atttcgcca gcgaagctc ctgttttca acaaaccaa aatccgtgg ggcaaagcc cccgatccg gccgaactg ccaatgcct gataaacta atgatgaaa ggtgtgtct gatgattcg actgattct ggtgttaat tcaaaattg gaaaattca ccgtctgat aacgaaact gaatcaggg gcttgcggg tcaggcgcc aaacgtcgt ttccccaat tatttgaac ttcgataag taagtatcg tctacttta agttgtcac tgtgattgt atttgttaa gttttttat tgttcaagt tggtgacac tgtcggttg gatgggtag cgttaccaa cttaattta ttgtctgta atttacttt ataatgtac tgatgaaag atttcgttg ctcagatgt tgtacaaat atttttata atcttcgtg actttgtaa ttatgttcc gtgtacttt aagttttac cagcattgt aaaattttt gaaaataaa taaatttgt acttt
