Status
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Gene information
General information
TC034053
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005776 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7860255..7874635
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034053
    iBeetle screen iB_00926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034053 RA
      MALTTCLKF FPRAQKRIH GEDVFGNKI KAVTPTLRS 
YTGRKVKDG AFQGARGQD KRFHEQEFG VRPMAQASS 
LMDVSYSTR RPRTPQETT LQQVLPNRV RRASGSSEY 
SDDGNKMLT DCSKPVDLI ISNLDPALN VKELRRLLT 
NMLKEYATI LSLKVVMQT DGVPVASIR VNNQQAAQF 
IISQLHRQK LGHKRIMVS YGQSNSPDP EELRAMVIS 
ILQEAPDNN MPLFKFMEL LESRFHCTV SVSEVNKLK 
DIIKISDNL GSRIISFTQ QMKIFPPPN LSKTLTPYC 
TIHCAGGLP NLGWCELNT NQSPNIMMS LRQFAAALD 
ELLTLHMDS MPLLSFQVC YEHVCHQPL PVDASGVPL 
EHLISCVPN VEIKNSGPN KNIKVIKRE LKGEVDEET 
VLKAVPPSL APNIILLCR EVVDLLKTT DRCQLLLSK 
FIPAYHHHF GRQCHVADY GYIKLIDLF ESIPHVVQI 
IGDGTRKII TLSHQAQMR RFTSDLLRV LKVQPAKQI 
TVGDFPAAY ERVMNRVFN PVDYGLCTF QDLLEEVPD 
NVVVITHND DNLVISVPK REQTAKEIM KTKQFAMEV 
IDILRHSPN CTMLFTKFV PAYHHHFGH QCRVSDYGF 
TKLIELFEA IPDVVKIQE LVEGERTIS LTLIQSLKI 
LGAQIVSFV KNSPTSSIL LDELPGIYL KECGYPLKP 
QVYESETLL ELFEKLSDY VQVVDTSSG RLVVAVDAN 
FLAVAKIRV WAILLKAPY RKEFSNFKY EYQSRYNSD 
IGINDLKQL RTIVTLSVD NDGIYIALT DLYVIAAQL 
YHLLYTNGG SILFTNLPR LYYQAFGKN VRLSEYKIN 
TIDQLYEQF DFLFFICGY KKSVLALNR NLAEYQVPV 
PSQICNWNH QTKKSAPPP ITKSSGAVR KYSPPKPDT 
PPTPNLAIE LPIIQNPQP MGNPYDLIS PAKLLFSTN 
QNPWGKAPD PAELPMPDK LMMKGVSDD STDSGVNSK 
LENSPSDNE TESGACGSG AKRRFPNYL NFDK
    CDS sequence
    • >TC034053 RA
      atggctctg accacgtgt ttgaaattt ttccctagg 
gcacagaaa cgaattcat ggagaggat gtcttcggg 
aacaagatc aaagctgtt acgccgacc ttgcggagt 
tacaccggg cggaaggtt aaggatggg gcgttccaa 
ggggctcgg gggcaagac aagaggttt cacgagcaa 
gaattcggc gtccgtcca atggcccaa gcatcgtcc 
cttatggac gtgagttac tcaactcgt cgtcctcgg 
acacctcag gaaaccacc ctccagcag gtgctgcca 
aatcgtgtt cgtcgcgca agcggaagt tccgaatac 
agcgacgat ggcaataaa atgctaact gattgttcc 
aaacccgtg gatcttatt atttcaaac ctggatcct 
gcactaaat gttaaagaa ttgcgaaga ttgctaaca 
aatatgttg aaggaatat gccacgatt ttgagtttg 
aaagtagtg atgcaaact gatggcgtt cccgtggct 
agcatcaga gtgaataat caacaggcg gctcagttt 
attatttcg cagcttcat agacaaaag ttggggcat 
aagaggatc atggtgtct tatggacag agtaattcc 
ccggatcca gaggagctg agggccatg gttataagc 
atattacag gaagccccc gacaacaac atgcccctt 
ttcaaattc atggaactg ctagaatcg cgtttccat 
tgcaccgtt tccgtttcg gaagtgaac aaactgaaa 
gacattatc aaaatcagc gacaatctc ggctctcgc 
atcatttca ttcacccaa caaatgaaa attttcccg 
ccaccgaac ttgagcaag actctaaca ccgtactgt 
acaatccac tgcgccggt ggtttaccc aatctcggt 
tggtgcgaa ctgaacacc aaccaaagc cccaacatc 
atgatgtct ttacgccag ttcgccgcc gccctcgac 
gaactcctc accctgcac atggattcc atgccatta 
ctgagtttc caggtctgt tacgagcac gtgtgccat 
cagccgctt ccggtggac gcctccggc gtgcctctg 
gagcacttg atctcgtgc gtgcccaac gtcgagatt 
aaaaactcc ggtccgaat aaaaacatc aaagttatt 
aagcgcgaa ttgaagggc gaagtcgac gaggagacg 
gtgttgaaa gctgtgccc ccgtctttg gcccccaac 
atcatcctg ctgtgtcgg gaggtggtc gatttgctc 
aaaaccacc gatagatgt cagctgctc ctcagtaaa 
tttatccca gcctaccac caccatttc gggcgtcag 
tgccacgtt gctgattac ggttacatc aaattaatt 
gatttattc gaatcaatt ccacatgtt gtgcaaata 
atcggcgac gggacgcga aaaatcatc acgctttcg 
catcaagcg caaatgcgc cgttttacg tcggatctg 
ttgcgagtg ttgaaagtc cagccggca aagcagatc 
actgtgggc gatttcccg gccgcctat gaacgggtc 
atgaaccgg gtgttcaat cccgttgat tatggatta 
tgcacgttt caagattta ctggaagag gtgcccgat 
aatgttgtg gtgattacg cataatgat gataatttg 
gtgatttcg gtgccgaaa cgcgaacaa accgccaaa 
gaaattatg aaaactaag caatttgca atggaggtt 
attgatatt ttgagacat tcccccaat tgtacgatg 
ctttttacc aagtttgtg ccggcgtat catcatcat 
tttggacat cagtgtagg gtctcggat tatgggttt 
accaagttg atcgagttg tttgaggcg atccctgat 
gtggtcaag attcaagag ttagtcgag ggtgaacgt 
accataagc cttacgttg atccaatcg ctcaagatt 
ttgggcgcg caaatagtg agttttgtg aagaattca 
ccgacctcg agtatttta ctcgacgaa cttccgggg 
atttacctg aaagagtgc ggctatccg ctcaagccg 
caagtgtac gaatcggag acgttgttg gagttgttt 
gagaaactc agtgattac gtccaggtt gttgatacg 
agttctggc cgtttggtg gtggccgtc gatgcgaat 
ttcctcgcc gttgccaaa attcgggtc tgggccatt 
ttactgaag gccccatat cgtaaagaa ttttccaat 
ttcaaatac gaatatcaa tcaaggtac aatagcgat 
attggaatc aacgatttg aaacagttg cgcactatt 
gtgacgtta tcggtggat aacgatgga atctacatc 
gctttaacc gatttatac gtcattgcg gcccaactg 
taccatcta ctgtacacc aatggtggg tccattttg 
ttcactaat ttaccaaga ctgtactat caagcattt 
ggcaaaaat gttcgactt tcggagtat aaaatcaac 
acgatcgat caattgtac gaacagttc gattttttg 
ttttttatc tgtggttac aagaagagt gtgcttgca 
ctgaaccgg aatttggcc gaatatcag gtacccgta 
ccttcgcaa atttgcaat tggaatcat cagacgaag 
aaatcggcg ccacctccg ataactaaa tcgtcaggg 
gcggttcgg aaatattct ccgcccaaa cccgacact 
ccgcccact cctaatttg gcaattgaa ttgcccata 
atccaaaat ccgcaaccg atgggtaat ccgtatgac 
ttgatttcg ccagcgaag ctcctgttt tcaacaaac 
caaaatccg tggggcaaa gcccccgat ccggccgaa 
ctgccaatg cctgataaa ctaatgatg aaaggtgtg 
tctgatgat tcgactgat tctggtgtt aattcaaaa 
ttggaaaat tcaccgtct gataacgaa actgaatca 
ggggcttgc gggtcaggc gccaaacgt cgtttcccc 
aattatttg aacttcgat aagtaa
    mRNA sequence
    • >TC034053 RA
      atgttaatg gctctgacc acgtgtttg aaatttttc 
cctagggca cagaaacga attcatgga gaggatgtc 
ttcgggaac aagatcaaa gctgttacg ccgaccttg 
cggagttac accgggcgg aaggttaag gatggggcg 
ttccaaggg gctcggggg caagacaag aggtttcac 
gagcaagaa ttcggcgtc cgtccaatg gcccaagca 
tcgtccctt atggacgtg agttactca actcgtcgt 
cctcggaca cctcaggaa accaccctc cagcaggtg 
ctgccaaat cgtgttcgt cgcgcaagc ggaagttcc 
gaatacagc gacgatggc aataaaatg ctaactgat 
tgttccaaa cccgtggat cttattatt tcaaacctg 
gatcctgca ctaaatgtt aaagaattg cgaagattg 
ctaacaaat atgttgaag gaatatgcc acgattttg 
agtttgaaa gtagtgatg caaactgat ggcgttccc 
gtggctagc atcagagtg aataatcaa caggcggct 
cagtttatt atttcgcag cttcataga caaaagttg 
gggcataag aggatcatg gtgtcttat ggacagagt 
aattccccg gatccagag gagctgagg gccatggtt 
ataagcata ttacaggaa gcccccgac aacaacatg 
ccccttttc aaattcatg gaactgcta gaatcgcgt 
ttccattgc accgtttcc gtttcggaa gtgaacaaa 
ctgaaagac attatcaaa atcagcgac aatctcggc 
tctcgcatc atttcattc acccaacaa atgaaaatt 
ttcccgcca ccgaacttg agcaagact ctaacaccg 
tactgtaca atccactgc gccggtggt ttacccaat 
ctcggttgg tgcgaactg aacaccaac caaagcccc 
aacatcatg atgtcttta cgccagttc gccgccgcc 
ctcgacgaa ctcctcacc ctgcacatg gattccatg 
ccattactg agtttccag gtctgttac gagcacgtg 
tgccatcag ccgcttccg gtggacgcc tccggcgtg 
cctctggag cacttgatc tcgtgcgtg cccaacgtc 
gagattaaa aactccggt ccgaataaa aacatcaaa 
gttattaag cgcgaattg aagggcgaa gtcgacgag 
gagacggtg ttgaaagct gtgcccccg tctttggcc 
cccaacatc atcctgctg tgtcgggag gtggtcgat 
ttgctcaaa accaccgat agatgtcag ctgctcctc 
agtaaattt atcccagcc taccaccac catttcggg 
cgtcagtgc cacgttgct gattacggt tacatcaaa 
ttaattgat ttattcgaa tcaattcca catgttgtg 
caaataatc ggcgacggg acgcgaaaa atcatcacg 
ctttcgcat caagcgcaa atgcgccgt tttacgtcg 
gatctgttg cgagtgttg aaagtccag ccggcaaag 
cagatcact gtgggcgat ttcccggcc gcctatgaa 
cgggtcatg aaccgggtg ttcaatccc gttgattat 
ggattatgc acgtttcaa gatttactg gaagaggtg 
cccgataat gttgtggtg attacgcat aatgatgat 
aatttggtg atttcggtg ccgaaacgc gaacaaacc 
gccaaagaa attatgaaa actaagcaa tttgcaatg 
gaggttatt gatattttg agacattcc cccaattgt 
acgatgctt tttaccaag tttgtgccg gcgtatcat 
catcatttt ggacatcag tgtagggtc tcggattat 
gggtttacc aagttgatc gagttgttt gaggcgatc 
cctgatgtg gtcaagatt caagagtta gtcgagggt 
gaacgtacc ataagcctt acgttgatc caatcgctc 
aagattttg ggcgcgcaa atagtgagt tttgtgaag 
aattcaccg acctcgagt attttactc gacgaactt 
ccggggatt tacctgaaa gagtgcggc tatccgctc 
aagccgcaa gtgtacgaa tcggagacg ttgttggag 
ttgtttgag aaactcagt gattacgtc caggttgtt 
gatacgagt tctggccgt ttggtggtg gccgtcgat 
gcgaatttc ctcgccgtt gccaaaatt cgggtctgg 
gccatttta ctgaaggcc ccatatcgt aaagaattt 
tccaatttc aaatacgaa tatcaatca aggtacaat 
agcgatatt ggaatcaac gatttgaaa cagttgcgc 
actattgtg acgttatcg gtggataac gatggaatc 
tacatcgct ttaaccgat ttatacgtc attgcggcc 
caactgtac catctactg tacaccaat ggtgggtcc 
attttgttc actaattta ccaagactg tactatcaa 
gcatttggc aaaaatgtt cgactttcg gagtataaa 
atcaacacg atcgatcaa ttgtacgaa cagttcgat 
tttttgttt tttatctgt ggttacaag aagagtgtg 
cttgcactg aaccggaat ttggccgaa tatcaggta 
cccgtacct tcgcaaatt tgcaattgg aatcatcag 
acgaagaaa tcggcgcca cctccgata actaaatcg 
tcaggggcg gttcggaaa tattctccg cccaaaccc 
gacactccg cccactcct aatttggca attgaattg 
cccataatc caaaatccg caaccgatg ggtaatccg 
tatgacttg atttcgcca gcgaagctc ctgttttca 
acaaaccaa aatccgtgg ggcaaagcc cccgatccg 
gccgaactg ccaatgcct gataaacta atgatgaaa 
ggtgtgtct gatgattcg actgattct ggtgttaat 
tcaaaattg gaaaattca ccgtctgat aacgaaact 
gaatcaggg gcttgcggg tcaggcgcc aaacgtcgt 
ttccccaat tatttgaac ttcgataag taagtatcg 
tctacttta agttgtcac tgtgattgt atttgttaa 
gttttttat tgttcaagt tggtgacac tgtcggttg 
gatgggtag cgttaccaa cttaattta ttgtctgta 
atttacttt ataatgtac tgatgaaag atttcgttg 
ctcagatgt tgtacaaat atttttata atcttcgtg 
actttgtaa ttatgttcc gtgtacttt aagttttac 
cagcattgt aaaattttt gaaaataaa taaatttgt 
acttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY