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Gene information
General information
TC034053
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005776
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 797025..811405
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034053
    iBeetle screen iB_00926
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034053 RA
      MALTTCLKF FPRAQKRIH GEDVFGNKI KAVTPTLRS 
      YTGRKVKDG AFQGARGQD KRFHEQEFG VRPMAQASS 
      LMDVSYSTR RPRTPQETT LQQVLPNRV RRASGSSEY 
      SDDGNKMLT DCSKPVDLI ISNLDPALN VKELRRLLT 
      NMLKEYATI LSLKVVMQT DGVPVASIR VNNQQAAQF 
      IISQLHRQK LGHKRIMVS YGQSNSPDP EELRAMVIS 
      ILQEAPDNN MPLFKFMEL LESRFHCTV SVSEVNKLK 
      DIIKISDNL GSRIISFTQ QMKIFPPPN LSKTLTPYC 
      TIHCAGGLP NLGWCELNT NQSPNIMMS LRQFAAALD 
      ELLTLHMDS MPLLSFQVC YEHVCHQPL PVDASGVPL 
      EHLISCVPN VEIKNSGPN KNIKVIKRE LKGEVDEET 
      VLKAVPPSL APNIILLCR EVVDLLKTT DRCQLLLSK 
      FIPAYHHHF GRQCHVADY GYIKLIDLF ESIPHVVQI 
      IGDGTRKII TLSHQAQMR RFTSDLLRV LKVQPAKQI 
      TVGDFPAAY ERVMNRVFN PVDYGLCTF QDLLEEVPD 
      NVVVITHND DNLVISVPK REQTAKEIM KTKQFAMEV 
      IDILRHSPN CTMLFTKFV PAYHHHFGH QCRVSDYGF 
      TKLIELFEA IPDVVKIQE LVEGERTIS LTLIQSLKI 
      LGAQIVSFV KNSPTSSIL LDELPGIYL KECGYPLKP 
      QVYESETLL ELFEKLSDY VQVVDTSSG RLVVAVDAN 
      FLAVAKIRV WAILLKAPY RKEFSNFKY EYQSRYNSD 
      IGINDLKQL RTIVTLSVD NDGIYIALT DLYVIAAQL 
      YHLLYTNGG SILFTNLPR LYYQAFGKN VRLSEYKIN 
      TIDQLYEQF DFLFFICGY KKSVLALNR NLAEYQVPV 
      PSQICNWNH QTKKSAPPP ITKSSGAVR KYSPPKPDT 
      PPTPNLAIE LPIIQNPQP MGNPYDLIS PAKLLFSTN 
      QNPWGKAPD PAELPMPDK LMMKGVSDD STDSGVNSK 
      LENSPSDNE TESGACGSG AKRRFPNYL NFDK
    CDS sequence
    • >TC034053 RA
      atggctctg accacgtgt ttgaaattt ttccctagg 
      gcacagaaa cgaattcat ggagaggat gtcttcggg 
      aacaagatc aaagctgtt acgccgacc ttgcggagt 
      tacaccggg cggaaggtt aaggatggg gcgttccaa 
      ggggctcgg gggcaagac aagaggttt cacgagcaa 
      gaattcggc gtccgtcca atggcccaa gcatcgtcc 
      cttatggac gtgagttac tcaactcgt cgtcctcgg 
      acacctcag gaaaccacc ctccagcag gtgctgcca 
      aatcgtgtt cgtcgcgca agcggaagt tccgaatac 
      agcgacgat ggcaataaa atgctaact gattgttcc 
      aaacccgtg gatcttatt atttcaaac ctggatcct 
      gcactaaat gttaaagaa ttgcgaaga ttgctaaca 
      aatatgttg aaggaatat gccacgatt ttgagtttg 
      aaagtagtg atgcaaact gatggcgtt cccgtggct 
      agcatcaga gtgaataat caacaggcg gctcagttt 
      attatttcg cagcttcat agacaaaag ttggggcat 
      aagaggatc atggtgtct tatggacag agtaattcc 
      ccggatcca gaggagctg agggccatg gttataagc 
      atattacag gaagccccc gacaacaac atgcccctt 
      ttcaaattc atggaactg ctagaatcg cgtttccat 
      tgcaccgtt tccgtttcg gaagtgaac aaactgaaa 
      gacattatc aaaatcagc gacaatctc ggctctcgc 
      atcatttca ttcacccaa caaatgaaa attttcccg 
      ccaccgaac ttgagcaag actctaaca ccgtactgt 
      acaatccac tgcgccggt ggtttaccc aatctcggt 
      tggtgcgaa ctgaacacc aaccaaagc cccaacatc 
      atgatgtct ttacgccag ttcgccgcc gccctcgac 
      gaactcctc accctgcac atggattcc atgccatta 
      ctgagtttc caggtctgt tacgagcac gtgtgccat 
      cagccgctt ccggtggac gcctccggc gtgcctctg 
      gagcacttg atctcgtgc gtgcccaac gtcgagatt 
      aaaaactcc ggtccgaat aaaaacatc aaagttatt 
      aagcgcgaa ttgaagggc gaagtcgac gaggagacg 
      gtgttgaaa gctgtgccc ccgtctttg gcccccaac 
      atcatcctg ctgtgtcgg gaggtggtc gatttgctc 
      aaaaccacc gatagatgt cagctgctc ctcagtaaa 
      tttatccca gcctaccac caccatttc gggcgtcag 
      tgccacgtt gctgattac ggttacatc aaattaatt 
      gatttattc gaatcaatt ccacatgtt gtgcaaata 
      atcggcgac gggacgcga aaaatcatc acgctttcg 
      catcaagcg caaatgcgc cgttttacg tcggatctg 
      ttgcgagtg ttgaaagtc cagccggca aagcagatc 
      actgtgggc gatttcccg gccgcctat gaacgggtc 
      atgaaccgg gtgttcaat cccgttgat tatggatta 
      tgcacgttt caagattta ctggaagag gtgcccgat 
      aatgttgtg gtgattacg cataatgat gataatttg 
      gtgatttcg gtgccgaaa cgcgaacaa accgccaaa 
      gaaattatg aaaactaag caatttgca atggaggtt 
      attgatatt ttgagacat tcccccaat tgtacgatg 
      ctttttacc aagtttgtg ccggcgtat catcatcat 
      tttggacat cagtgtagg gtctcggat tatgggttt 
      accaagttg atcgagttg tttgaggcg atccctgat 
      gtggtcaag attcaagag ttagtcgag ggtgaacgt 
      accataagc cttacgttg atccaatcg ctcaagatt 
      ttgggcgcg caaatagtg agttttgtg aagaattca 
      ccgacctcg agtatttta ctcgacgaa cttccgggg 
      atttacctg aaagagtgc ggctatccg ctcaagccg 
      caagtgtac gaatcggag acgttgttg gagttgttt 
      gagaaactc agtgattac gtccaggtt gttgatacg 
      agttctggc cgtttggtg gtggccgtc gatgcgaat 
      ttcctcgcc gttgccaaa attcgggtc tgggccatt 
      ttactgaag gccccatat cgtaaagaa ttttccaat 
      ttcaaatac gaatatcaa tcaaggtac aatagcgat 
      attggaatc aacgatttg aaacagttg cgcactatt 
      gtgacgtta tcggtggat aacgatgga atctacatc 
      gctttaacc gatttatac gtcattgcg gcccaactg 
      taccatcta ctgtacacc aatggtggg tccattttg 
      ttcactaat ttaccaaga ctgtactat caagcattt 
      ggcaaaaat gttcgactt tcggagtat aaaatcaac 
      acgatcgat caattgtac gaacagttc gattttttg 
      ttttttatc tgtggttac aagaagagt gtgcttgca 
      ctgaaccgg aatttggcc gaatatcag gtacccgta 
      ccttcgcaa atttgcaat tggaatcat cagacgaag 
      aaatcggcg ccacctccg ataactaaa tcgtcaggg 
      gcggttcgg aaatattct ccgcccaaa cccgacact 
      ccgcccact cctaatttg gcaattgaa ttgcccata 
      atccaaaat ccgcaaccg atgggtaat ccgtatgac 
      ttgatttcg ccagcgaag ctcctgttt tcaacaaac 
      caaaatccg tggggcaaa gcccccgat ccggccgaa 
      ctgccaatg cctgataaa ctaatgatg aaaggtgtg 
      tctgatgat tcgactgat tctggtgtt aattcaaaa 
      ttggaaaat tcaccgtct gataacgaa actgaatca 
      ggggcttgc gggtcaggc gccaaacgt cgtttcccc 
      aattatttg aacttcgat aagtaa
    mRNA sequence
    • >TC034053 RA
      atgttaatg gctctgacc acgtgtttg aaatttttc 
      cctagggca cagaaacga attcatgga gaggatgtc 
      ttcgggaac aagatcaaa gctgttacg ccgaccttg 
      cggagttac accgggcgg aaggttaag gatggggcg 
      ttccaaggg gctcggggg caagacaag aggtttcac 
      gagcaagaa ttcggcgtc cgtccaatg gcccaagca 
      tcgtccctt atggacgtg agttactca actcgtcgt 
      cctcggaca cctcaggaa accaccctc cagcaggtg 
      ctgccaaat cgtgttcgt cgcgcaagc ggaagttcc 
      gaatacagc gacgatggc aataaaatg ctaactgat 
      tgttccaaa cccgtggat cttattatt tcaaacctg 
      gatcctgca ctaaatgtt aaagaattg cgaagattg 
      ctaacaaat atgttgaag gaatatgcc acgattttg 
      agtttgaaa gtagtgatg caaactgat ggcgttccc 
      gtggctagc atcagagtg aataatcaa caggcggct 
      cagtttatt atttcgcag cttcataga caaaagttg 
      gggcataag aggatcatg gtgtcttat ggacagagt 
      aattccccg gatccagag gagctgagg gccatggtt 
      ataagcata ttacaggaa gcccccgac aacaacatg 
      ccccttttc aaattcatg gaactgcta gaatcgcgt 
      ttccattgc accgtttcc gtttcggaa gtgaacaaa 
      ctgaaagac attatcaaa atcagcgac aatctcggc 
      tctcgcatc atttcattc acccaacaa atgaaaatt 
      ttcccgcca ccgaacttg agcaagact ctaacaccg 
      tactgtaca atccactgc gccggtggt ttacccaat 
      ctcggttgg tgcgaactg aacaccaac caaagcccc 
      aacatcatg atgtcttta cgccagttc gccgccgcc 
      ctcgacgaa ctcctcacc ctgcacatg gattccatg 
      ccattactg agtttccag gtctgttac gagcacgtg 
      tgccatcag ccgcttccg gtggacgcc tccggcgtg 
      cctctggag cacttgatc tcgtgcgtg cccaacgtc 
      gagattaaa aactccggt ccgaataaa aacatcaaa 
      gttattaag cgcgaattg aagggcgaa gtcgacgag 
      gagacggtg ttgaaagct gtgcccccg tctttggcc 
      cccaacatc atcctgctg tgtcgggag gtggtcgat 
      ttgctcaaa accaccgat agatgtcag ctgctcctc 
      agtaaattt atcccagcc taccaccac catttcggg 
      cgtcagtgc cacgttgct gattacggt tacatcaaa 
      ttaattgat ttattcgaa tcaattcca catgttgtg 
      caaataatc ggcgacggg acgcgaaaa atcatcacg 
      ctttcgcat caagcgcaa atgcgccgt tttacgtcg 
      gatctgttg cgagtgttg aaagtccag ccggcaaag 
      cagatcact gtgggcgat ttcccggcc gcctatgaa 
      cgggtcatg aaccgggtg ttcaatccc gttgattat 
      ggattatgc acgtttcaa gatttactg gaagaggtg 
      cccgataat gttgtggtg attacgcat aatgatgat 
      aatttggtg atttcggtg ccgaaacgc gaacaaacc 
      gccaaagaa attatgaaa actaagcaa tttgcaatg 
      gaggttatt gatattttg agacattcc cccaattgt 
      acgatgctt tttaccaag tttgtgccg gcgtatcat 
      catcatttt ggacatcag tgtagggtc tcggattat 
      gggtttacc aagttgatc gagttgttt gaggcgatc 
      cctgatgtg gtcaagatt caagagtta gtcgagggt 
      gaacgtacc ataagcctt acgttgatc caatcgctc 
      aagattttg ggcgcgcaa atagtgagt tttgtgaag 
      aattcaccg acctcgagt attttactc gacgaactt 
      ccggggatt tacctgaaa gagtgcggc tatccgctc 
      aagccgcaa gtgtacgaa tcggagacg ttgttggag 
      ttgtttgag aaactcagt gattacgtc caggttgtt 
      gatacgagt tctggccgt ttggtggtg gccgtcgat 
      gcgaatttc ctcgccgtt gccaaaatt cgggtctgg 
      gccatttta ctgaaggcc ccatatcgt aaagaattt 
      tccaatttc aaatacgaa tatcaatca aggtacaat 
      agcgatatt ggaatcaac gatttgaaa cagttgcgc 
      actattgtg acgttatcg gtggataac gatggaatc 
      tacatcgct ttaaccgat ttatacgtc attgcggcc 
      caactgtac catctactg tacaccaat ggtgggtcc 
      attttgttc actaattta ccaagactg tactatcaa 
      gcatttggc aaaaatgtt cgactttcg gagtataaa 
      atcaacacg atcgatcaa ttgtacgaa cagttcgat 
      tttttgttt tttatctgt ggttacaag aagagtgtg 
      cttgcactg aaccggaat ttggccgaa tatcaggta 
      cccgtacct tcgcaaatt tgcaattgg aatcatcag 
      acgaagaaa tcggcgcca cctccgata actaaatcg 
      tcaggggcg gttcggaaa tattctccg cccaaaccc 
      gacactccg cccactcct aatttggca attgaattg 
      cccataatc caaaatccg caaccgatg ggtaatccg 
      tatgacttg atttcgcca gcgaagctc ctgttttca 
      acaaaccaa aatccgtgg ggcaaagcc cccgatccg 
      gccgaactg ccaatgcct gataaacta atgatgaaa 
      ggtgtgtct gatgattcg actgattct ggtgttaat 
      tcaaaattg gaaaattca ccgtctgat aacgaaact 
      gaatcaggg gcttgcggg tcaggcgcc aaacgtcgt 
      ttccccaat tatttgaac ttcgataag taagtatcg 
      tctacttta agttgtcac tgtgattgt atttgttaa 
      gttttttat tgttcaagt tggtgacac tgtcggttg 
      gatgggtag cgttaccaa cttaattta ttgtctgta 
      atttacttt ataatgtac tgatgaaag atttcgttg 
      ctcagatgt tgtacaaat atttttata atcttcgtg 
      actttgtaa ttatgttcc gtgtacttt aagttttac 
      cagcattgt aaaattttt gaaaataaa taaatttgt 
      acttt