Status
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Gene information
General information
TC005769 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7913138..7916654
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005769
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00923

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005769 RA
      MALNLFIKR SLSTSSPMN AVIKNVTVI GGGLMGSGI 
      AQVAAQTGH NVTIVEVNP DLIKKAEDS IAKNLVRVA 
      KKLHKDDPQ AANKFVSES QARIKGCTD IAEAVKDSD 
      LVVEAIVEN MDVKHKLFK NLDEVAPPK TMFASNTSS 
      LSITEIASV TKRKDRFGG LHFFNPVPV MKLLEVVRT 
      PETSQETYE AFMAWGKAV GKTCVTCKD TPGFIVNRL 
      LVPYIAEAI RMMERGDAS PRDIDTAMK LGAGYPMGP 
      IELADYVGH DTSHAIMQG WHKKFPDNP LFKPNESVQ 
      KLIDEKKLG VKTGEGFYK YTK
    CDS sequence
    • >TC005769 RA
      atggcccta aacctgttc atcaagcga agcctttca 
      acttcaagc cccatgaac gcagttata aaaaacgta 
      actgtgatc gggggcggc ctcatgggc tcaggcatc 
      gcccaagtg gccgcgcaa accggccac aacgtgacc 
      atcgtggaa gtcaacccc gacttgatc aaaaaagcc 
      gaagacagc attgcgaaa aacttggta cgagtcgcc 
      aaaaaactc cacaaggat gacccgcaa gccgctaat 
      aaattcgtg agcgaatcg caggcccgg attaagggg 
      tgcaccgac attgccgaa gctgtgaaa gacagcgat 
      ttggtcgtg gaggcgatt gttgaaaac atggatgtt 
      aagcacaaa ttgttcaaa aatttggat gaggtcgcg 
      ccccccaaa accatgttt gcgtcaaat acgagctcg 
      ctctcgatt accgaaatt gcctccgtt acgaagagg 
      aaggatagg ttcgggggg ctgcatttt ttcaatccc 
      gtgcccgtt atgaagctt ttggaggtt gttaggact 
      ccggagacg tcgcaggag acgtacgag gcctttatg 
      gcgtggggc aaagccgtg ggcaagacc tgcgtcacc 
      tgcaaggac acccccggg tttattgtt aacaggctt 
      ctggtgccc tatatagca gaagccatc aggatgatg 
      gagagaggt gatgcttcg ccccgcgat atcgacacc 
      gccatgaag ctgggggct ggctacccc atgggcccc 
      atcgaactg gccgactac gtgggacat gacacctcg 
      cacgccatc atgcaaggc tggcacaag aaattccct 
      gataacccc cttttcaaa ccgaacgaa tccgtacaa 
      aaactaatc gatgagaaa aaactgggc gtgaaaacc 
      ggcgaaggc ttctacaaa tacaccaaa taa
    mRNA sequence
    • >TC005769 RA
      gtaccactt gactgaatg atgttgcac ttttattac 
      ataatacct aaataaatt caggtacaa aagtcgcac 
      tcgttatcg aaagcggat tatttttaa accatcggc 
      acgatgatt ttcacttct gagccctaa tctcgccat 
      aaacgataa aatgattaa aaagtacac aaacgtagc 
      ggtgggagt cgatcatag gttacaaac ttggacttt 
      cacctagtt attgtatcg ttaataaac caaacaaaa 
      tccgcatta cacaccact ttttactcg taaccgaac 
      gcgaaaaaa tggccctaa acctgttca tcaagcgaa 
      gcctttcaa cttcaagcc ccatgaacg cagttataa 
      aaaacgtaa ctgtgatcg ggggcggcc tcatgggct 
      caggcatcg cccaagtgg ccgcgcaaa ccggccaca 
      acgtgacca tcgtggaag tcaaccccg acttgatca 
      aaaaagccg aagacagca ttgcgaaaa acttggtac 
      gagtcgcca aaaaactcc acaaggatg acccgcaag 
      ccgctaata aattcgtga gcgaatcgc aggcccgga 
      ttaaggggt gcaccgaca ttgccgaag ctgtgaaag 
      acagcgatt tggtcgtgg aggcgattg ttgaaaaca 
      tggatgtta agcacaaat tgttcaaaa atttggatg 
      aggtcgcgc cccccaaaa ccatgtttg cgtcaaata 
      cgagctcgc tctcgatta ccgaaattg cctccgtta 
      cgaagagga aggataggt tcggggggc tgcattttt 
      tcaatcccg tgcccgtta tgaagcttt tggaggttg 
      ttaggactc cggagacgt cgcaggaga cgtacgagg 
      cctttatgg cgtggggca aagccgtgg gcaagacct 
      gcgtcacct gcaaggaca cccccgggt ttattgtta 
      acaggcttc tggtgccct atatagcag aagccatca 
      ggatgatgg agagaggtg atgcttcgc cccgcgata 
      tcgacaccg ccatgaagc tgggggctg gctacccca 
      tgggcccca tcgaactgg ccgactacg tgggacatg 
      acacctcgc acgccatca tgcaaggct ggcacaaga 
      aattccctg ataaccccc ttttcaaac cgaacgaat 
      ccgtacaaa aactaatcg atgagaaaa aactgggcg 
      tgaaaaccg gcgaaggct tctacaaat acaccaaat 
      aaatatcta tatgtattt taatcaaat aaacaattt 
      tttaattga tttgtttac tgatgtcga tttttcctc 
      gtgtaaaat actgttggc aaagccgcg aaggttcga 
      tcggatgtc attcatgcc gttctattt tcgtaaacc 
      gagtgattt attttattg aaaaggtct cctcgagtc 
      gcaaaaagt cagtcatcg ttttattat cgtcgtatt 
      gtcgctata aaaacacat ctttaatgt gtgttctta 
      aagtggagt gaaggacga cttaaaaat acatgcaca 
      gagattggg cgaattagc ggcgagtac acgattttt 
      tcgagtaaa taaactttc gcgatttcc ggttgaaaa 
      aacaattaa cacgtgggt gcgaacgtt gataacatt 
      tccgtgaaa tcgcagtca atgcaatcg atacatcct 
      gtttgagga gatgagcga aatttattt cggcggtaa 
      gttgagggc ggttctcgg taattcccc taataaaca 
      cgtcatggc aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY