Status
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Gene information
General information
TC005758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8206832..8210269
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005758
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00921

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 6

    metathoracic leg tarsus structure irregular - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005758 RA
      MPKFDKKRT RNRFNPSFR GKTNRPQVH REYQHQNQL 
REIDVGITE FINTFEGFS GVIKARFSD FQVNEIDPD 
GQIAKLTNL EIPKFGTTV EKADAPADS PLSQIPQET 
WLGLKQMLD EAEPPLIEL DVSEMTKED RTEIHKSLK 
KYFGGKISA NTETKNDKK FMVFKKHDG KIDNRSNWP 
SDKGEFVHF LVYKECLDT LDTALRIAS CLKMPPAHF 
SYAGVKDRR AKTTQWFSV KRVEPWKLM VKTRPIRNV 
KIGNITFKD KPLKLGDLT GNKFRIALR NVTGDDDLI 
EKSLKSLQD KGFVNYYGL QRFGNDKEV PTFSVGIQL 
LLGNWKEAC DIILKLTKS DQPDLDVSK AKKVYAETG 
DPEKALAQF ESKNSCIEY LLLSGLSKE HKNDYVNAL 
ASIPRNTRL LYIHSFQSL VWNLIASKR IEKYGLKVV 
PGDLILLEN LKENSEDDE DCGENKKEP VKELTDEEC 
ANYTIYDIV LPLPGYDIT YPENMKDCY KEILEKYGL 
TLEMTRQKV RTYTLSGTY RKLMGQVQN LSWKIMYYN 
DPNDNLIRS DLEELKGEK EPASVEGGK YKALLLDFC 
LDSSSYATM VLREILKSD TSSSSHAKL NNYHVATAK 
PEEKTCEEN EFSDKVGLL NDSQKYEAF KNIVFSEDD 
TSLKRKCEE ENGEKAKVA KT
    CDS sequence
    • >TC005758 RA
      atgccaaaa ttcgacaaa aaacgcacc agaaaccgc 
ttcaatccc agctttagg ggaaaaacc aaccgaccg 
caagtccac cgcgaatac cagcaccaa aatcaattg 
cgcgaaatc gatgtggga attaccgaa ttcattaac 
acgtttgag ggtttctct ggcgtgatc aaagcccgc 
ttttcggat tttcaagtc aatgaaatc gaccccgac 
ggccaaatt gccaaacta acaaacttg gaaatcccc 
aagtttggg acaaccgtg gaaaaagcc gatgcaccg 
gctgatagc cccttgtcc caaatcccg caggagaca 
tggctaggt ttaaagcaa atgcttgac gaggcggag 
ccgcctttg atcgaactc gacgtcagt gaaatgaca 
aaagaggat cgtacggaa attcacaaa tcgttgaaa 
aagtacttt ggagggaaa attagtgca aatactgag 
acgaaaaac gataagaaa tttatggtt tttaaaaaa 
cacgacggt aaaattgac aaccgctcg aactggccg 
agtgataaa ggggagttt gtccatttc cttgtgtat 
aaggaatgt ctcgacacg ttagacacc gccttaagg 
atcgcctca tgtttaaaa atgcccccc gcacatttt 
agttatgcc ggggttaag gaccgaaga gcgaaaacc 
actcaatgg ttttcggtc aaaagagtc gaaccgtgg 
aaactgatg gtaaaaacg cgaccaatt cgaaacgta 
aagattggt aatatcact ttcaaagac aagccgtta 
aaactaggc gacttgacc ggtaataaa ttccgaatc 
gcgctcaga aatgtgact ggggacgac gatttgatc 
gaaaagtcg ctcaaaagt ctccaagac aaggggttt 
gttaattat tacgggtta caaaggttt gggaacgat 
aaagaagtg cccacgttt tcggtcggg atacaactt 
ttgttgggc aattggaaa gaggcctgt gatattatt 
ttgaaacta acaaagtca gaccagccc gatttggac 
gtctctaaa gctaagaaa gtatacgct gagacgggc 
gaccctgaa aaggcttta gctcaattc gagagcaaa 
aattcttgt atcgaatat ttattattg tcaggactg 
agcaaagaa cataaaaat gactacgtc aatgcttta 
gcgagtata ccgcgaaat acgagactc ctttacatt 
cactcgttc caaagtttg gtttggaat ttaattgcg 
tcaaaacga attgaaaaa tacggcctt aaggtcgtc 
ccaggcgac ttgattctt ctcgaaaat ttaaaagaa 
aactcggaa gatgacgag gattgtggc gaaaataaa 
aaagagccc gtgaaagaa ctaaccgat gaagagtgc 
gccaattat acaatttat gacattgtt ttaccactg 
ccgggttac gacattaca tatccggaa aatatgaaa 
gactgttac aaggaaatt ttagaaaaa tacggccta 
actttagaa atgacgaga caaaaagta cgcacttat 
acgttaagt ggaacttat cgtaaatta atggggcaa 
gtgcagaac ctgtcgtgg aaaataatg tactataac 
gaccccaac gataattta attaggtca gatttggaa 
gaactgaaa ggggagaaa gaacccgcg agtgttgaa 
ggaggaaaa tacaaagcg ctgctgcta gatttttgc 
ctcgactca tcttcatat gccacaatg gtgttacgc 
gaaattcta aaaagcgat acttcgagt agctcccac 
gcgaaatta aataattac catgtggca acagcaaaa 
cctgaggaa aaaacgtgt gaggaaaat gagttttcg 
gataaagtt ggattactc aacgactca caaaagtac 
gaagctttc aaaaatata gtcttcagt gaagacgat 
acaagtttg aaacgaaag tgtgaggag gagaatggg 
gaaaaggca aaggtcgcg aaaacgtaa
    mRNA sequence
    • >TC005758 RA
      taaacgtaa ccacaacca cataacctc tcatttttt 
gactgtcac tttgacagt tgtggcggt ggttaaaca 
cgtgtgata aaaaatgcc aaaattcga caaaaaacg 
caccagaaa ccgcttcaa tcccagctt taggggaaa 
aaccaaccg accgcaagt ccaccgcga ataccagca 
ccaaaatca attgcgcga aatcgatgt gggaattac 
cgaattcat taacacgtt tgagggttt ctctggcgt 
gatcaaagc ccgcttttc ggattttca agtcaatga 
aatcgaccc cgacggcca aattgccaa actaacaaa 
cttggaaat ccccaagtt tgggacaac cgtggaaaa 
agccgatgc accggctga tagcccctt gtcccaaat 
cccgcagga gacatggct aggtttaaa gcaaatgct 
tgacgaggc ggagccgcc tttgatcga actcgacgt 
cagtgaaat gacaaaaga ggatcgtac ggaaattca 
caaatcgtt gaaaaagta ctttggagg gaaaattag 
tgcaaatac tgagacgaa aaacgataa gaaatttat 
ggtttttaa aaaacacga cggtaaaat tgacaaccg 
ctcgaactg gccgagtga taaagggga gtttgtcca 
tttccttgt gtataagga atgtctcga cacgttaga 
caccgcctt aaggatcgc ctcatgttt aaaaatgcc 
ccccgcaca ttttagtta tgccggggt taaggaccg 
aagagcgaa aaccactca atggttttc ggtcaaaag 
agtcgaacc gtggaaact gatggtaaa aacgcgacc 
aattcgaaa cgtaaagat tggtaatat cactttcaa 
agacaagcc gttaaaact aggcgactt gaccggtaa 
taaattccg aatcgcgct cagaaatgt gactgggga 
cgacgattt gatcgaaaa gtcgctcaa aagtctcca 
agacaaggg gtttgttaa ttattacgg gttacaaag 
gtttgggaa cgataaaga agtgcccac gttttcggt 
cgggataca acttttgtt gggcaattg gaaagaggc 
ctgtgatat tattttgaa actaacaaa gtcagacca 
gcccgattt ggacgtctc taaagctaa gaaagtata 
cgctgagac gggcgaccc tgaaaaggc tttagctca 
attcgagag caaaaattc ttgtatcga atatttatt 
attgtcagg actgagcaa agaacataa aaatgacta 
cgtcaatgc tttagcgag tataccgcg aaatacgag 
actccttta cattcactc gttccaaag tttggtttg 
gaatttaat tgcgtcaaa acgaattga aaaatacgg 
ccttaaggt cgtcccagg cgacttgat tcttctcga 
aaatttaaa agaaaactc ggaagatga cgaggattg 
tggcgaaaa taaaaaaga gcccgtgaa agaactaac 
cgatgaaga gtgcgccaa ttatacaat ttatgacat 
tgttttacc actgccggg ttacgacat tacatatcc 
ggaaaatat gaaagactg ttacaagga aattttaga 
aaaatacgg cctaacttt agaaatgac gagacaaaa 
agtacgcac ttatacgtt aagtggaac ttatcgtaa 
attaatggg gcaagtgca gaacctgtc gtggaaaat 
aatgtacta taacgaccc caacgataa tttaattag 
gtcagattt ggaagaact gaaagggga gaaagaacc 
cgcgagtgt tgaaggagg aaaatacaa agcgctgct 
gctagattt ttgcctcga ctcatcttc atatgccac 
aatggtgtt acgcgaaat tctaaaaag cgatacttc 
gagtagctc ccacgcgaa attaaataa ttaccatgt 
ggcaacagc aaaacctga ggaaaaaac gtgtgagga 
aaatgagtt ttcggataa agttggatt actcaacga 
ctcacaaaa gtacgaagc tttcaaaaa tatagtctt 
cagtgaaga cgatacaag tttgaaacg aaagtgtga 
ggaggagaa tggggaaaa ggcaaaggt cgcgaaaac 
gtaactaca ggaaacttt tttatcggc ttgaaataa 
aaacactt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY