Status
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Gene information
General information
TC005757 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8213551..8221769
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005757
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005757 RA
      MMGGKSKYM INGMSVQNK KVQDLFCSI QLNVNNPHF 
      LIMQGKITK VLNMKPFEI LGMIEEGAG TKMYTTKRD 
      DTLKTIRKK DAKLTELQT IMKEVITPR LEKLREERR 
      QYIEFKNVE KELDHMLGL YKAWEYVVS NRRYVEMKN 
      KLEAEENSL KNVEEEKTL HKERIKELD GIIKELTKK 
      AEADGNKGL EKLENELRT AEKSQAKVN ANIKSLNDE 
      IDNETKRKK QLEKNFSDD EKTLKLKES NLAKVESTF 
      LKLKEADEK DKEAYLTSQ KRVVALSAG MELNDQGEA 
      ESLLAQLMN VRQEVSEVT SNKNCTRVK LKYFEDRLK 
      EKQRKSSNA NEIHKDQQA QKAMTQEIE QLKNSMKKL 
      HFKEDWMND LKNRRGQLA QEMRVLKQR VDHFEMRNS 
      YTQFRYKDP EPNFKRSSV IGVVCRLFE VKDEAAAYA 
      LEMAAGGRL YNVVIDTDI TGKKLLKNG DLQQRRTFM 
      PLNKIQVNK MSNSVVKTA ESLVGKDNI KLALDYLKY 
      DKKMQVVME HIFGNVFIC KDMDVAKQV AFDNRIRRK 
      CVTLDGDIV DPAGTLTGG SREQTESVL KQLETIKQV 
      EQQLNAKEE EFEKIESEI REMGPKEEQ FATYRRQLE 
      LKEHELSLI TQRLQQSTY YQQKEEIAN LEKEIEHLK 
      QTIASCEEN EKKLNEKVA SLEKQVEGS KGGTEKKLK 
      EAEAEMARL KAKADKSRK EWQQKELDY ATFKGEIEE 
      LKQSLLDTK QQIEAAEAN IEKLKQQLE EIGTQSTEM 
      NENIAELQA QLKKGKAEI VEKNKDVQK KINEKEELQ 
      SKITQCEIQ MKESTHKLK KLQDECKNL KTRQADCEQ 
      RANRNELKN AEKMSDEDG LKLERKIRE AQEMRKNLG 
      RTVNSQAQV HFEEQEKEY NEVKKKLRI VEQDKQKLL 
      DAIKELDRK REDIVCKAY SQISKDFGS IFSTLLPGA 
      AAKLLPPTG QTVLQGVEI KVSLGGIWK DSLTELSGG 
      QRSLAALSL ILAMLLFKP APLYILDEV DAALDLSHT 
      QNIGRMLKT HFKTSQFIV VSLKDGMFS NANVLFKTQ 
      FVDGVSTVS RTVNRSAV
    CDS sequence
    • >TC005757 RA
      atgatgggg gggaaaagt aaatacatg attaacgga 
      atgtcggtt caaaataag aaagtccaa gatttgttt 
      tgttccatt caattgaac gtgaataat ccacacttt 
      ttgattatg caagggaaa atcaccaaa gtcttaaac 
      atgaaaccg tttgaaatt ttaggcatg attgaggaa 
      ggcgctggc actaaaatg tatacaact aaacgagac 
      gatactttg aaaacaatt cggaaaaaa gatgccaaa 
      cttacggaa ctccaaact ataatgaaa gaggtaatc 
      acaccaaga ttagaaaaa ttaagagag gaacgaaga 
      caatacatt gagttcaaa aacgtagaa aaggaatta 
      gaccacatg ttaggtctc tacaaagct tgggaatat 
      gtcgtgtcg aacagaaga tacgtagaa atgaaaaac 
      aaactcgaa gccgaagaa aacagtttg aaaaatgtt 
      gaggaagaa aaaacactc cataaagaa agaattaaa 
      gagctggat gggattatt aaggaatta acaaagaag 
      gccgaggcg gatggtaat aaaggtttg gaaaaactc 
      gaaaacgaa ctacgaacg gctgaaaaa tcccaagcc 
      aaagtgaac gcaaacata aaatcgctc aacgacgaa 
      attgacaac gaaaccaag agaaaaaag caactggaa 
      aagaatttt tccgacgac gaaaaaacc ctcaaactc 
      aaagaatcc aacttggcg aaagtcgaa tcgactttc 
      ctcaaactc aaagaagcg gacgaaaag gacaaggaa 
      gcttacctc acgtcccaa aaacgagtt gtggcttta 
      agcgctgga atggaactc aacgaccaa ggcgaagcc 
      gagtcgctt ctcgcccaa ttaatgaac gtacgtcaa 
      gaagtctct gaagtgacc agcaacaaa aactgcact 
      cgcgtcaag ctcaaatat tttgaagac cgcctcaaa 
      gaaaaacaa cggaaaagt tccaacgcc aacgaaatc 
      cacaaagac caacaggcc caaaaggcc atgactcaa 
      gaaattgaa caattgaag aacagtatg aaaaaattg 
      catttcaag gaggactgg atgaacgat ttgaagaat 
      aggagagga cagttggcg caagaaatg agggttttg 
      aagcaacgg gttgatcat tttgagatg cggaattca 
      tacacccag ttcaggtat aaagacccc gaacccaat 
      tttaaaagg agcagtgtt attggagtt gtttgccga 
      ttgtttgaa gtgaaagac gaggcggcg gcgtatgct 
      ttggaaatg gcagccggt ggtcggttg tacaatgtt 
      gttatagat accgacatt accggcaag aagctactc 
      aaaaacggg gatttacag cagaggcgc accttcatg 
      ccgcttaat aaaattcaa gtgaataag atgagcaac 
      agtgttgtt aaaacagcc gagagttta gtcgggaaa 
      gacaatatc aaactggcc ttggactac ttgaagtac 
      gacaaaaaa atgcaagtc gttatggaa cacattttc 
      ggaaatgtg ttcatttgt aaggacatg gatgtggca 
      aaacaagtc gcctttgat aacaggatt agacgcaaa 
      tgtgtgacg ctagatggc gacattgtc gacccggcg 
      ggtacctta actgggggt tcccgggaa caaaccgaa 
      tcggttttg aaacaactc gaaaccata aaacaagtg 
      gagcaacaa ctgaatgcc aaagaagaa gaatttgaa 
      aaaatcgag tccgaaatt cgtgaaatg ggccccaaa 
      gaagaacaa ttcgccacg tatcgaaga cagttggaa 
      ttgaaagag cacgaattg agtttaatt acgcaaagg 
      ttgcaacaa tcgacttat taccaacag aaggaagaa 
      atagcgaat ttggaaaaa gaaatcgaa catttgaag 
      caaacaatt gcctcgtgt gaagaaaat gagaagaaa 
      ctcaacgaa aaagtggcc agtttggag aaacaagtc 
      gaaggctcg aaaggtgga accgagaag aaattaaaa 
      gaggctgaa gcagaaatg gccagattg aaggcaaaa 
      gcggacaaa agccgaaaa gagtggcaa caaaaagag 
      ttggattat gcaacgttt aagggagaa attgaagaa 
      ttgaaacag agtttgttg gatacgaag cagcagatt 
      gaagcagct gaagcaaat atcgaaaaa ttaaaacag 
      caacttgaa gaaatcggg acgcaatca accgaaatg 
      aatgaaaac atcgccgaa ttgcaagcc caactcaag 
      aaaggcaaa gccgaaatc gtcgagaag aacaaagac 
      gtgcaaaag aagatcaac gaaaaagaa gaactgcag 
      tcgaaaatc acccagtgt gagatccaa atgaaagaa 
      tccactcac aagttgaag aaactacaa gacgagtgt 
      aagaatttg aagacgcgt caagctgat tgtgagcaa 
      cgtgccaat cgcaacgag ttgaaaaac gccgagaag 
      atgtcagat gaagatggc ctaaagttg gagagaaaa 
      attcgagaa gcacaagaa atgaggaaa aaccttgga 
      cgcacggtc aacagccaa gcacaagtc catttcgaa 
      gaacaggaa aaagagtac aatgaagtg aagaaaaag 
      ttacgaatt gttgaacaa gacaaacag aagctgctc 
      gatgccatt aaagaatta gacaggaag agagaagat 
      attgtgtgc aaggcttat tcgcaaatt tccaaagat 
      tttggttcg attttcagc acgttgttg ccaggggcc 
      gccgccaag ctgttgccg cccacggga caaacggtc 
      cttcaggga gttgagatt aaggtctcg cttggtggc 
      atttggaaa gacagtttg acggaattg agtggagga 
      caaaggtca ttagcagcc ctgtcgtta attctcgcc 
      atgttgtta ttcaaaccg gcgccattg tatatttta 
      gatgaagtt gacgccgcc ttagatctg tcacatacg 
      cagaatatt ggtcgtatg ttaaagact catttcaaa 
      acgtcacag ttcattgtg gtgtcgctc aaagacgga 
      atgttcagt aacgctaat gttcttttc aaaacacaa 
      ttcgtagat ggcgtttcg accgtctca aggaccgtc 
      aaccggagt gccgtttaa
    mRNA sequence
    • >TC005757 RA
      ccacgcacc gaagtttcg ggttttgat aaggattca 
      acgctatca cgggcctaa atggcagtg gtaaatcaa 
      acatcctcg attccatat gtttcgttc taggaatct 
      cgaatttga gccatgtcc gtgtgggca atttgcaag 
      acttgattt acaaaagtg gccaatgcg gtattgaaa 
      aggccacgg tgtctatca cttttgata attcgaacc 
      cagatcagt gccccccag ggtttgaag atgtctccg 
      agatcacca tctcaagac agatcatga tggggggga 
      aaagtaaat acatgatta acggaatgt cggttcaaa 
      ataagaaag tccaagatt tgttttgtt ccattcaat 
      tgaacgtga ataatccac actttttga ttatgcaag 
      ggaaaatca ccaaagtct taaacatga aaccgtttg 
      aaattttag gcatgattg aggaaggcg ctggcacta 
      aaatgtata caactaaac gagacgata ctttgaaaa 
      caattcgga aaaaagatg ccaaactta cggaactcc 
      aaactataa tgaaagagg taatcacac caagattag 
      aaaaattaa gagaggaac gaagacaat acattgagt 
      tcaaaaacg tagaaaagg aattagacc acatgttag 
      gtctctaca aagcttggg aatatgtcg tgtcgaaca 
      gaagatacg tagaaatga aaaacaaac tcgaagccg 
      aagaaaaca gtttgaaaa atgttgagg aagaaaaaa 
      cactccata aagaaagaa ttaaagagc tggatggga 
      ttattaagg aattaacaa agaaggccg aggcggatg 
      gtaataaag gtttggaaa aactcgaaa acgaactac 
      gaacggctg aaaaatccc aagccaaag tgaacgcaa 
      acataaaat cgctcaacg acgaaattg acaacgaaa 
      ccaagagaa aaaagcaac tggaaaaga atttttccg 
      acgacgaaa aaaccctca aactcaaag aatccaact 
      tggcgaaag tcgaatcga ctttcctca aactcaaag 
      aagcggacg aaaaggaca aggaagctt acctcacgt 
      cccaaaaac gagttgtgg ctttaagcg ctggaatgg 
      aactcaacg accaaggcg aagccgagt cgcttctcg 
      cccaattaa tgaacgtac gtcaagaag tctctgaag 
      tgaccagca acaaaaact gcactcgcg tcaagctca 
      aatattttg aagaccgcc tcaaagaaa aacaacgga 
      aaagttcca acgccaacg aaatccaca aagaccaac 
      aggcccaaa aggccatga ctcaagaaa ttgaacaat 
      tgaagaaca gtatgaaaa aattgcatt tcaaggagg 
      actggatga acgatttga agaatagga gaggacagt 
      tggcgcaag aaatgaggg ttttgaagc aacgggttg 
      atcattttg agatgcgga attcataca cccagttca 
      ggtataaag accccgaac ccaatttta aaaggagca 
      gtgttattg gagttgttt gccgattgt ttgaagtga 
      aagacgagg cggcggcgt atgctttgg aaatggcag 
      ccggtggtc ggttgtaca atgttgtta tagataccg 
      acattaccg gcaagaagc tactcaaaa acggggatt 
      tacagcaga ggcgcacct tcatgccgc ttaataaaa 
      ttcaagtga ataagatga gcaacagtg ttgttaaaa 
      cagccgaga gtttagtcg ggaaagaca atatcaaac 
      tggccttgg actacttga agtacgaca aaaaaatgc 
      aagtcgtta tggaacaca ttttcggaa atgtgttca 
      tttgtaagg acatggatg tggcaaaac aagtcgcct 
      ttgataaca ggattagac gcaaatgtg tgacgctag 
      atggcgaca ttgtcgacc cggcgggta ccttaactg 
      ggggttccc gggaacaaa ccgaatcgg ttttgaaac 
      aactcgaaa ccataaaac aagtggagc aacaactga 
      atgccaaag aagaagaat ttgaaaaaa tcgagtccg 
      aaattcgtg aaatgggcc ccaaagaag aacaattcg 
      ccacgtatc gaagacagt tggaattga aagagcacg 
      aattgagtt taattacgc aaaggttgc aacaatcga 
      cttattacc aacagaagg aagaaatag cgaatttgg 
      aaaaagaaa tcgaacatt tgaagcaaa caattgcct 
      cgtgtgaag aaaatgaga agaaactca acgaaaaag 
      tggccagtt tggagaaac aagtcgaag gctcgaaag 
      gtggaaccg agaagaaat taaaagagg ctgaagcag 
      aaatggcca gattgaagg caaaagcgg acaaaagcc 
      gaaaagagt ggcaacaaa aagagttgg attatgcaa 
      cgtttaagg gagaaattg aagaattga aacagagtt 
      tgttggata cgaagcagc agattgaag cagctgaag 
      caaatatcg aaaaattaa aacagcaac ttgaagaaa 
      tcgggacgc aatcaaccg aaatgaatg aaaacatcg 
      ccgaattgc aagcccaac tcaagaaag gcaaagccg 
      aaatcgtcg agaagaaca aagacgtgc aaaagaaga 
      tcaacgaaa aagaagaac tgcagtcga aaatcaccc 
      agtgtgaga tccaaatga aagaatcca ctcacaagt 
      tgaagaaac tacaagacg agtgtaaga atttgaaga 
      cgcgtcaag ctgattgtg agcaacgtg ccaatcgca 
      acgagttga aaaacgccg agaagatgt cagatgaag 
      atggcctaa agttggaga gaaaaattc gagaagcac 
      aagaaatga ggaaaaacc ttggacgca cggtcaaca 
      gccaagcac aagtccatt tcgaagaac aggaaaaag 
      agtacaatg aagtgaaga aaaagttac gaattgttg 
      aacaagaca aacagaagc tgctcgatg ccattaaag 
      aattagaca ggaagagag aagatattg tgtgcaagg 
      cttattcgc aaatttcca aagattttg gttcgattt 
      tcagcacgt tgttgccag gggccgccg ccaagctgt 
      tgccgccca cgggacaaa cggtccttc agggagttg 
      agattaagg tctcgcttg gtggcattt ggaaagaca 
      gtttgacgg aattgagtg gaggacaaa ggtcattag 
      cagccctgt cgttaattc tcgccatgt tgttattca 
      aaccggcgc cattgtata ttttagatg aagttgacg 
      ccgccttag atctgtcac atacgcaga atattggtc 
      gtatgttaa agactcatt tcaaaacgt cacagttca 
      ttgtggtgt cgctcaaag acggaatgt tcagtaacg 
      ctaatgttc ttttcaaaa cacaattcg tagatggcg 
      tttcgaccg tctcaagga ccgtcaacc ggagtgccg 
      tttaagtat tactaagag tgtagatga ttatttcct 
      aaataaatt ttttaccca cga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY