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Gene information
General information
TC005741 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 1204643..1207400
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005741
    iBeetle screen iB_00917
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005741 RA
      MYLVFCLMF GVAFASGRD YAEKGNEHF SAVAYQAAK 
      RAGYPGYRY SYYVISPIL IEIVFAIYY LGSAGGTRE 
      NLGELFGFS LKNAEVEER FEYLVPLLS QNGFRMAHK 
      MIALRGTEW QPTWQKKVE DIFHLQFEV FDDSDEPAE 
      KIERWFQEH TNNKMERMI YELHQLQWA VTHLLLLSA 
      SYFQANWTD TSATKTVMA RYFNYGPLH KKVGKFLEL 
      PFEKSEASM VFVYPETVS DFKILEDEI EYIFKGPHF 
      NRSEPTTFS VPKFRFEYA IMEYSIHHL RKMGFGGGV 
      DWTFLTKFY SFPFSNITD NNDRAWINA FTEKVYMDL 
      NEKGVEIAD GLIAQFEDT TYIYNNIVD LPFIFYIKL 
      RQTIVFMGR KINFKIIDK EKSNNK
    CDS sequence
    • >TC005741 RA
      atgtatttg gtcttttgt ttaatgttt ggtgtcgca 
      tttgcgtct ggacgtgat tatgctgaa aagggtaat 
      gaacacttt tccgcagtt gcgtaccag gccgcgaaa 
      cgtgcgggg tatcccggt taccgttac agttactat 
      gtcataagt cctatcttg attgagata gttttcgct 
      atttattac ttgggaagc gccggaggg actagagaa 
      aacttgggt gaacttttt ggtttttcc cttaaaaat 
      gcagaagtt gaagaaaga tttgaatac ttggtcccg 
      cttcttagc cagaatggt ttccgcatg gcacataag 
      atgattgcg ttacgcgga accgaatgg cagccgact 
      tggcagaaa aaagtggag gacattttc catttgcag 
      tttgaggtt ttcgatgat tctgatgag ccggcagaa 
      aaaattgaa cgttggttt caagagcac accaataat 
      aaaatggaa agaatgatt tatgagcta catcagctg 
      caatgggct gtaacacat ctcttactc ctttcggct 
      tcatatttt caagccaac tggactgac acgtctgcc 
      acaaaaact gtcatggcg aggtatttc aattacgga 
      ccgcttcac aaaaaagtt ggcaaattt ctggaatta 
      ccgttcgaa aaaagtgaa gcttctatg gtttttgtc 
      tatccggaa acagtcagc gactttaag atacttgaa 
      gacgaaatt gagtacatt tttaaaggg ccgcacttt 
      aataggtct gaaccaacg actttttca gtgccgaaa 
      tttcgattt gaatatgct attatggag tattctatc 
      caccatcta cgaaaaatg ggttttgga ggaggtgtt 
      gattggaca tttttaaca aagttctat agctttccc 
      tttagcaac attacagat aataatgac agagcgtgg 
      atcaacgcc tttacggaa aaagtttat atggatctc 
      aatgaaaaa ggagttgag atagcggat ggtctgata 
      gcacagttt gaggacaca acttatata tataacaat 
      attgtcgat cttcctttc atattttac atcaaatta 
      agacaaact attgtcttt atgggccgc aaaattaat 
      tttaaaata atcgacaaa gaaaaatcc aataataaa 
      taa
    mRNA sequence
    • >TC005741 RA
      gaagccttc agtcaaaaa atgtatttg gtcttttgt 
      ttaatgttt ggtgtcgca tttgcgtct ggacgtgat 
      tatgctgaa aagggtaat gaacacttt tccgcagtt 
      gcgtaccag gccgcgaaa cgtgcgggg tatcccggt 
      taccgttac agttactat gtcataagt cctatcttg 
      attgagata gttttcgct atttattac ttgggaagc 
      gccggaggg actagagaa aacttgggt gaacttttt 
      ggtttttcc cttaaaaat gcagaagtt gaagaaaga 
      tttgaatac ttggtcccg cttcttagc cagaatggt 
      ttccgcatg gcacataag atgattgcg ttacgcgga 
      accgaatgg cagccgact tggcagaaa aaagtggag 
      gacattttc catttgcag tttgaggtt ttcgatgat 
      tctgatgag ccggcagaa aaaattgaa cgttggttt 
      caagagcac accaataat aaaatggaa agaatgatt 
      tatgagcta catcagctg caatgggct gtaacacat 
      ctcttactc ctttcggct tcatatttt caagccaac 
      tggactgac acgtctgcc acaaaaact gtcatggcg 
      aggtatttc aattacgga ccgcttcac aaaaaagtt 
      ggcaaattt ctggaatta ccgttcgaa aaaagtgaa 
      gcttctatg gtttttgtc tatccggaa acagtcagc 
      gactttaag atacttgaa gacgaaatt gagtacatt 
      tttaaaggg ccgcacttt aataggtct gaaccaacg 
      actttttca gtgccgaaa tttcgattt gaatatgct 
      attatggag tattctatc caccatcta cgaaaaatg 
      ggttttgga ggaggtgtt gattggaca tttttaaca 
      aagttctat agctttccc tttagcaac attacagat 
      aataatgac agagcgtgg atcaacgcc tttacggaa 
      aaagtttat atggatctc aatgaaaaa ggagttgag 
      atagcggat ggtctgata gcacagttt gaggacaca 
      acttatata tataacaat attgtcgat cttcctttc 
      atattttac atcaaatta agacaaact attgtcttt 
      atgggccgc aaaattaat tttaaaata atcgacaaa 
      gaaaaatcc aataataaa taaaatttg tgcaacaca 
      agtttagtg cagtttaat ttccccca