Status
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Gene information
General information
TC005725 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8437711..8449397
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005725
    iBeetle screen iB_00914

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 3
    mature egg not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005725 RA
      MAHCMRVLH RQGGKASSA LLTEIQIRC FHVSPLAAA 
      ASKVAMSKF DKDGLPYDK LIKNLEIVK KRLNRPLTL 
      SEKVLYSHL DDPQNQDVQ RGTSYLRLR PDRVAMQDA 
      TAQMAMLQF ISSGLKRVA VPSTIHCDH LIEAQVGGE 
      KDLARAKDL NQEVYSFLS SAGNKYGVG FWKPGSGII 
      HQIILENYA FPGLLMIGT DSHTPNGGG LGGLCIGVG 
      GADAVDVMA NIPWELKCP NVIGVKLTG KLGGWTSPK 
      DIILKVADI LTVKGGTGA IVEYFGPGV DSISCTGMA 
      TICNMGAEI GATTSIFPY NSRMSDYLK ATKRGEIAG 
      EADKVKSAL LNRDEDCTY DQVIEINLD TLEPHVNGP 
      FTPDLANPI SKLGEAAKK NGWPVDIKV GLIGSCTNS 
      SYEDMGRCA SIVKEAMNH GLKSKIPFN VTPGSEQIR 
      ATIERDGIA KTLRDFGGT VLANACGPC IGQWDRKDV 
      KKGEKNTIV TSYNRNFTG RNDANPATH SFVTSPELV 
      TALSIAGRL DFDPTKDEL TGKDGKKFK LSSPFGDEL 
      PTKGFDPGQ DTYEAPVAD GSTVKVDVS PTSQRLQLL 
      EPFDKWNGK DLEDLVVLI KVKGKCTTD HISAAGPWL 
      KYRGHLDNI SNNMFIGAT NAENGEMNK IKNQLTGEW 
      GAVPDVARH YKANKIGWV AIGDDNYGE GSSREHAAL 
      EPRHLGGRA IIVKSFARI HETNLKKQG LLPLTFSNP 
      SDYDKIQPT DKISLKGLA SLTPGKPVE CEIKHADGK 
      VEKILLNHS LNELQIGWF KAGSALNRM KELAASA
    CDS sequence
    • >TC005725 RA
      atggcgcac tgtatgcga gttttgcat cgccagggt 
      ggcaaggcc agcagtgct ttgctgacc gaaatccaa 
      ataagatgt ttccacgtt tcgccactg gcagcggcc 
      gcctccaaa gtggctatg agcaaattc gacaaggac 
      ggacttccc tatgataag ctaatcaaa aatctagaa 
      attgtcaaa aagaggctc aaccgcccc ttgactctt 
      tcggaaaaa gtgctctac tcccatctg gacgacccc 
      caaaatcag gatgtccaa cgtggcacg tcttatttg 
      cgcttacgc ccggaccgt gtggctatg caagacgcc 
      accgcccag atggccatg ttacaattt atttccagc 
      ggtttgaaa cgtgtcgct gtcccatca accattcac 
      tgcgaccac ttgatcgaa gcccaagtg ggcggcgaa 
      aaggacttg gcccgcgct aaagacctc aaccaggag 
      gtttacagt ttcctaagc agcgccggt aacaaatac 
      ggcgtcggt ttctggaaa ccgggctcc ggaatcatc 
      caccaaatc atcctcgaa aattatgcc ttccctggc 
      cttctcatg atcggcacc gactcgcac acccccaat 
      gggggcggt ctgggcggc ctgtgcatc ggtgtcggc 
      ggcgccgac gccgtggac gtcatggcc aacatcccc 
      tgggagctg aaatgtccc aacgtgatc ggtgtcaaa 
      ctcaccggg aaattgggc ggttggacg tcccccaag 
      gacatcatc ctgaaagtg gccgatatt ttgaccgtt 
      aaagggggc actggcgct atcgttgag tacttcggg 
      cctggggtt gattccatt tcgtgcacc gggatggca 
      accatttgc aacatgggg gctgaaatt ggggccaca 
      acttccatt ttcccgtac aatagtcgc atgagcgac 
      tacttgaag gccaccaag cgaggggag atcgccgga 
      gaggctgat aaagttaaa agtgcgctt ttgaaccgt 
      gatgaagac tgcacgtac gatcaagtg attgagatt 
      aatcttgat acgcttgaa ccgcatgtt aatgggccg 
      tttacgccc gatttggcg aatccgatc agtaagttg 
      ggagaagcg gcgaagaaa aatgggtgg ccggttgat 
      atcaaagtc gggttgatt gggtcgtgt actaatagc 
      tcgtacgag gatatgggc aggtgtgcg agtatcgtc 
      aaagaggcg atgaatcat gggttgaaa tcgaagatt 
      ccgttcaat gtcacgccg ggatcggag cagatcagg 
      gcgacgatt gaaagggat ggaattgcc aagactttg 
      agggatttc ggaggcacg gtattggcc aacgcttgt 
      ggcccgtgc atcgggcaa tgggaccgc aaagacgtg 
      aaaaagggc gaaaagaac accattgta acctcatac 
      aatcgtaac ttcaccggt cgtaacgac gccaatccg 
      gcgacacat tctttcgtc acatcaccg gagctggtc 
      acagctcta agtattgcg ggccgattg gacttcgac 
      cccactaaa gatgaactg accggaaaa gacggcaag 
      aaattcaaa ttgagttcg cccttcggg gacgaactc 
      cctaccaaa ggtttcgac cccggtcaa gacacctac 
      gaagccccc gtggctgac ggaagcacc gtcaaggtt 
      gacgtcagc cccacgtca cagcggttg caactgttg 
      gaacctttc gacaagtgg aacgggaag gatttggag 
      gatttggtc gttttgatc aaagtcaag gggaaatgc 
      acgactgat cacatttcg gcagctggg ccgtggctg 
      aagtacaga gggcatctc gataatatt tccaacaac 
      atgtttatt ggcgcgaca aatgcggaa aacggcgaa 
      atgaacaag atcaagaac cagctgacg ggcgagtgg 
      ggcgcagtc cccgacgtt gcgcgccac tacaaggcc 
      aacaaaata gggtgggtg gccatcggc gacgataat 
      tacggggag ggaagttcg cgggagcat gccgccttg 
      gagccccga catctcggc ggtcgggcc attattgtt 
      aaatctttc gcgcgtatc cacgaaacc aatctcaaa 
      aaacagggg ctcttgcct ttgacgttc tcaaatccc 
      agcgattac gataagatt caacctacc gataaaatt 
      agtcttaaa ggtctcgcg agtttaact ccagggaag 
      cccgttgag tgtgagatt aaacatgcc gatggaaag 
      gtcgagaaa attttactt aatcattcg cttaatgag 
      ctacaaatt gggtggttt aaggccgga agcgcgctc 
      aacaggatg aaggagttg gcggcgtcg gcgtaa
    mRNA sequence
    • >TC005725 RA
      aagcaacaa aaaaaatta tttggaatt tttttttaa 
      ataaagcaa aaaacgtaa ctactgcat tttgcgtta 
      atatgaaag ttcgaaaag ccacaaact tgcagagga 
      atatatttt gggccaaaa gactgattt ttgacacga 
      ctgtcattt agtgttatg ccaaaagtg gatgtcatt 
      aaaaaaatt catcgtaaa ttttcagaa gtacttcct 
      gttgaaacg tgaacctgg ccctcggta tcacacacg 
      agcaaaagt cgcgatagg gggactatt tgaaaacaa 
      gttattaat ggaataaaa ttagtgaat gcgtgcgca 
      cggagccgg ccagctgag aacagatca aatttaggg 
      tacatacgt aagagtgta cttcacaat taccagcca 
      acaccattt ctaactgct ttggggccc ttttgcaac 
      catggcgca ctgtatgcg agttttgca tcgccaggg 
      tggcaaggc cagcagtgc tttgctgac cgaaatcca 
      aataagatg tttccacgt ttcgccact ggcagcggc 
      cgcctccaa agtggctat gagcaaatt cgacaagga 
      cggacttcc ctatgataa gctaatcaa aaatctaga 
      aattgtcaa aaagaggct caaccgccc cttgactct 
      ttcggaaaa agtgctcta ctcccatct ggacgaccc 
      ccaaaatca ggatgtcca acgtggcac gtcttattt 
      gcgcttacg cccggaccg tgtggctat gcaagacgc 
      caccgccca gatggccat gttacaatt tatttccag 
      cggtttgaa acgtgtcgc tgtcccatc aaccattca 
      ctgcgacca cttgatcga agcccaagt gggcggcga 
      aaaggactt ggcccgcgc taaagacct caaccagga 
      ggtttacag tttcctaag cagcgccgg taacaaata 
      cggcgtcgg tttctggaa accgggctc cggaatcat 
      ccaccaaat catcctcga aaattatgc cttccctgg 
      ccttctcat gatcggcac cgactcgca cacccccaa 
      tgggggcgg tctgggcgg cctgtgcat cggtgtcgg 
      cggcgccga cgccgtgga cgtcatggc caacatccc 
      ctgggagct gaaatgtcc caacgtgat cggtgtcaa 
      actcaccgg gaaattggg cggttggac gtcccccaa 
      ggacatcat cctgaaagt ggccgatat tttgaccgt 
      taaaggggg cactggcgc tatcgttga gtacttcgg 
      gcctggggt tgattccat ttcgtgcac cgggatggc 
      aaccatttg caacatggg ggctgaaat tggggccac 
      aacttccat tttcccgta caatagtcg catgagcga 
      ctacttgaa ggccaccaa gcgagggga gatcgccgg 
      agaggctga taaagttaa aagtgcgct tttgaaccg 
      tgatgaaga ctgcacgta cgatcaagt gattgagat 
      taatcttga tacgcttga accgcatgt taatgggcc 
      gtttacgcc cgatttggc gaatccgat cagtaagtt 
      gggagaagc ggcgaagaa aaatgggtg gccggttga 
      tatcaaagt cgggttgat tgggtcgtg tactaatag 
      ctcgtacga ggatatggg caggtgtgc gagtatcgt 
      caaagaggc gatgaatca tgggttgaa atcgaagat 
      tccgttcaa tgtcacgcc gggatcgga gcagatcag 
      ggcgacgat tgaaaggga tggaattgc caagacttt 
      gagggattt cggaggcac ggtattggc caacgcttg 
      tggcccgtg catcgggca atgggaccg caaagacgt 
      gaaaaaggg cgaaaagaa caccattgt aacctcata 
      caatcgtaa cttcaccgg tcgtaacga cgccaatcc 
      ggcgacaca ttctttcgt cacatcacc ggagctggt 
      cacagctct aagtattgc gggccgatt ggacttcga 
      ccccactaa agatgaact gaccggaaa agacggcaa 
      gaaattcaa attgagttc gcccttcgg ggacgaact 
      ccctaccaa aggtttcga ccccggtca agacaccta 
      cgaagcccc cgtggctga cggaagcac cgtcaaggt 
      tgacgtcag ccccacgtc acagcggtt gcaactgtt 
      ggaaccttt cgacaagtg gaacgggaa ggatttgga 
      ggatttggt cgttttgat caaagtcaa ggggaaatg 
      cacgactga tcacatttc ggcagctgg gccgtggct 
      gaagtacag agggcatct cgataatat ttccaacaa 
      catgtttat tggcgcgac aaatgcgga aaacggcga 
      aatgaacaa gatcaagaa ccagctgac gggcgagtg 
      gggcgcagt ccccgacgt tgcgcgcca ctacaaggc 
      caacaaaat agggtgggt ggccatcgg cgacgataa 
      ttacgggga gggaagttc gcgggagca tgccgcctt 
      ggagccccg acatctcgg cggtcgggc cattattgt 
      taaatcttt cgcgcgtat ccacgaaac caatctcaa 
      aaaacaggg gctcttgcc tttgacgtt ctcaaatcc 
      cagcgatta cgataagat tcaacctac cgataaaat 
      tagtcttaa aggtctcgc gagtttaac tccagggaa 
      gcccgttga gtgtgagat taaacatgc cgatggaaa 
      ggtcgagaa aattttact taatcattc gcttaatga 
      gctacaaat tgggtggtt taaggccgg aagcgcgct 
      caacaggat gaaggagtt ggcggcgtc ggcgtaaat 
      tgattcatg attttcttg tggtttaga aagcacttg 
      aggtacttt tgtattatc cgattgtag agtcattga 
      tcaattgtg ctgtacata gtttttatt cctttttca 
      aattttttt gtgcaagta tatttaata aaacatttg 
      acaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY