Status
Please wait ...
Gene information
General information
TC005723 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8495726..8498688
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005723
    iBeetle screen iB_00913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005723 RA
      MSEKSAAIR TQLSNIIAS GGSHKDQAE KYRGILENI 
      LAGSGPELT EGLQIFIEA IVNENVSLV ISRQILTEI 
      SSHLMKLPD DVSKTVSHF MLEKVQPRV ISFEEQVAS 
      IRQHLADIY ERNHMWREA AAVLVGIPL ETGQKQYTV 
      DYKLETYLK IARLYLEDD DPVQAEAFI NRASLLQAE 
      SRNEQLQIY YKVCYARVL DYRRKFIEA AQRYNELSY 
      RTIVHEDER MTALRNALV CTVLASAGQ QRSRMLATL 
      FKDERCQQL PAVAILEKM YLERIIRRS ELRDFEALL 
      QPHQKASTI DGSTILDRA VIEHNLLSA SKLYNNISF 
      EELGALLEI HPSKAEKIA SQMITEGRM NGYIDQIDS 
      IVHFETRET LPQWDKQIQ SLCYQVNSI IESIAKSEP 
      EWISKVMEE QMDLK
    CDS sequence
    • >TC005723 RA
      atgtcggag aaaagcgcg gctatccgc acccaactc 
      tccaacata atcgcgtcc gggggctcc cacaaagac 
      caggccgaa aaatatcgc ggtattctt gagaacatc 
      ctcgccggt tcgggcccc gagctgacc gaaggcttg 
      cagatattc atcgaagca attgtcaat gagaacgtt 
      agtttggtc atctcgcgc caaatcctg accgaaata 
      agctcgcat ttgatgaaa ctacccgac gatgtttcc 
      aaaactgtc tcacacttt atgcttgag aaggtgcaa 
      ccccgggtt atttcgttt gaggagcag gtggcaagt 
      attcgccaa catttggcc gatatctac gaacgtaac 
      cacatgtgg cgcgaggct gcagctgtc ctggtggga 
      atcccgctc gaaaccggc cagaaacaa tacaccgtg 
      gattacaaa cttgaaacg tatttgaaa atcgcgagg 
      ttgtatctt gaggatgac gatccggtc caggctgag 
      gctttcatc aaccgggcg tcgctgctt caggccgag 
      tcacggaat gagcagctt caaatttat tacaaagtt 
      tgttatgct cgagttttg gattatcgg cggaagttt 
      attgaggcg gctcagaga tacaatgaa ttgtcctat 
      aggacgatt gttcatgaa gatgaacgc atgacagcc 
      cttcggaat gctttagtt tgcactgtt ttggcttca 
      gcggggcaa caaagatcg cgcatgttg gccacactg 
      ttcaaggac gagaggtgc caacagttg ccagcagtg 
      gcgattttg gaaaaaatg tacctcgaa agaatcatc 
      aggaggtcc gaattgagg gattttgag gccttgttg 
      cagccccac caaaaggcg agcaccatt gatgggtca 
      acaattctg gaccgcgcc gttatcgaa cacaatctc 
      ctgtctgct agtaaactt tataataat attagtttc 
      gaagagttg ggagctttg ctcgaaata cacccttcc 
      aaggctgaa aaaatcgca agtcagatg ataaccgaa 
      gggcgaatg aacggttac attgaccaa atcgattca 
      atcgtccac tttgaaact cgtgaaact ttgccccag 
      tgggacaaa caaatccag tctctctgc taccaggtg 
      aattcaatc attgaatca atcgcaaaa agcgagccc 
      gaatggatc agtaaggtg atggaggag cagatggat 
      cttaagtga
    mRNA sequence
    • >TC005723 RA
      ttgctgttt tgtcagtca tttgtcaaa aactacttt 
      tagttaaat taaaataat ttattatta tttgtggcc 
      aaaatagta gcggagcac catgtcgga gaaaagcgc 
      ggctatccg cacccaact ctccaacat aatcgcgtc 
      cgggggctc ccacaaaga ccaggccga aaaatatcg 
      cggtattct tgagaacat cctcgccgg ttcgggccc 
      cgagctgac cgaaggctt gcagatatt catcgaagc 
      aattgtcaa tgagaacgt tagtttggt catctcgcg 
      ccaaatcct gaccgaaat aagctcgca tttgatgaa 
      actacccga cgatgtttc caaaactgt ctcacactt 
      tatgcttga gaaggtgca accccgggt tatttcgtt 
      tgaggagca ggtggcaag tattcgcca acatttggc 
      cgatatcta cgaacgtaa ccacatgtg gcgcgaggc 
      tgcagctgt cctggtggg aatcccgct cgaaaccgg 
      ccagaaaca atacaccgt ggattacaa acttgaaac 
      gtatttgaa aatcgcgag gttgtatct tgaggatga 
      cgatccggt ccaggctga ggctttcat caaccgggc 
      gtcgctgct tcaggccga gtcacggaa tgagcagct 
      tcaaattta ttacaaagt ttgttatgc tcgagtttt 
      ggattatcg gcggaagtt tattgaggc ggctcagag 
      atacaatga attgtccta taggacgat tgttcatga 
      agatgaacg catgacagc ccttcggaa tgctttagt 
      ttgcactgt tttggcttc agcggggca acaaagatc 
      gcgcatgtt ggccacact gttcaagga cgagaggtg 
      ccaacagtt gccagcagt ggcgatttt ggaaaaaat 
      gtacctcga aagaatcat caggaggtc cgaattgag 
      ggattttga ggccttgtt gcagcccca ccaaaaggc 
      gagcaccat tgatgggtc aacaattct ggaccgcgc 
      cgttatcga acacaatct cctgtctgc tagtaaact 
      ttataataa tattagttt cgaagagtt gggagcttt 
      gctcgaaat acacccttc caaggctga aaaaatcgc 
      aagtcagat gataaccga agggcgaat gaacggtta 
      cattgacca aatcgattc aatcgtcca ctttgaaac 
      tcgtgaaac tttgcccca gtgggacaa acaaatcca 
      gtctctctg ctaccaggt gaattcaat cattgaatc 
      aatcgcaaa aagcgagcc cgaatggat cagtaaggt 
      gatggagga gcagatgga tcttaagtg aaacgtgtc 
      ttttgtaaa ttacatttt atttgtttt acaatactt 
      gtcataatc ataaaacaa ttgaattaa aagcttgca 
      caagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY