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Gene information
General information
TC005722 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8502091..8505608
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005722
    iBeetle screen iB_00912

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005722 RA
      MSFASKSIY ATLPRTQRG QPLVLGGDP KGKNFLYTN 
      GNSVIIRNI QNPAIADVY TEHSCPVNV AKYSPSGFY 
      IASGDQSGK VRIWDTVNK EHILKNEFH PIGGPIKDI 
      AWSPDNQRM VVVGEGRER FGHVFMSET GTSVGEISG 
      QSKPINSCD FKPTRPFRI ITGSEDNTI AVFEGPPFK 
      FKMTKQEHS RFVQAVRYS PTGNLFASA GFDGKVFLY 
      DGTSSDLVG EVGSPAHSG GVYGVSWSP DGKQLLTAS 
      GDKTAKLWD VETRQVVSE FVLGSTVED QQVSCLWQN 
      EYLLTVSLS GFINYLDIN DPTKPLRVV KGHNKPITV 
      LELSEDRGT IFTGSHDGN VTRWNAETG ENDRVEGVG 
      HGNQINGMR VHGGTLYTC GIDDSLKQI DIEGNTYKG 
      QDLKLGSQP RGMDILKEQ NVVVTASVN EITVSQDNR 
      KASTLKVSY EPSSVSVSP RGHVAVGGA MDNKVHLYE 
      LKGTELVPL QELDHLGAV TDVAYSPDD KYLVACDAH 
      RKVVLYSVP EYKLATNQE WGFHNARVN SVAWSPDSL 
      LVASGSLDT SIIIWSVEK PAKHIIIKN AHAQSQVTR 
      LAWLDDQTL VSVGQDCNT KLWTITKF
    CDS sequence
    • >TC005722 RA
      atgtcattt gcatcaaag agtatttac gcgactttg 
      ccgcggacc caacgagga cagcccctg gtgttgggc 
      ggggatccc aaaggcaag aactttctc tacaccaat 
      ggcaattcc gtgatcatt cgcaacatc caaaaccct 
      gcaattgcc gacgtctac acggaacat tcctgtccg 
      gtaaacgtc gccaagtac tcccctagc ggcttctac 
      atcgcctct ggagaccaa tcgggcaaa gtcagaatc 
      tgggacaca gtcaacaag gagcacatc cttaaaaat 
      gagtttcac ccaatcggg gggcccatc aaagatatc 
      gcttggtcc cctgataac caacgaatg gtcgtggtt 
      ggcgaaggc cgcgaacgc ttcggtcat gttttcatg 
      tccgaaacg ggaacttcc gttggcgaa atctcaggc 
      caatcaaaa cccatcaat tcctgcgat ttcaaaccc 
      acgagacct ttcagaatc atcacgggg tctgaagac 
      aacaccatt gcggttttc gaggggccc ccgtttaag 
      ttcaaaatg accaagcag gagcacagc aggtttgtg 
      caagccgtt cgttattca cccaccggt aatttattt 
      gcctcggct gggttcgat ggtaaggtg tttctgtac 
      gatggtacg tcctccgat ttggtgggg gaagtggga 
      agccccgcc cacagcggg ggcgtgtat ggggttagt 
      tggagtccc gacggtaaa caattgcta acagccagt 
      ggggacaaa actgcgaaa ttgtgggac gtggaaacg 
      agacaagtc gtctctgaa ttcgtccta ggttcgaca 
      gttgaagat caacaagtt tcgtgttta tggcaaaac 
      gaatacttg ctcacggtt tctttgagt ggttttatt 
      aattatttg gatattaat gacccaacg aagccgctc 
      cgggttgtt aaagggcac aataaaccc atcactgtc 
      ttagaattg agcgaagat cgtggtacg atctttacc 
      gggtcgcat gatggtaat gtgactcga tggaacgcc 
      gaaactggg gagaacgat cgggttgag ggcgtgggt 
      catggtaac cagatcaat gggatgagg gtacatggt 
      ggtactttg tacacttgt ggtattgat gactcttta 
      aaacaaatc gacattgag ggcaacacg tacaaaggg 
      caagatttg aagttgggg tcgcaacct cgcggaatg 
      gacatttta aaagagcaa aatgtggtg gtcacagcg 
      agtgttaac gaaatcacc gtctcgcaa gataacagg 
      aaagcaagc actttgaag gtgtcttat gaaccgtca 
      agtgtcagc gtttcgccg cggggtcac gtggctgtg 
      ggtggtgcc atggacaac aaggtgcat ttatacgaa 
      ctgaaaggc acagagttg gtacccttg caagaattg 
      gaccatttg ggtgccgta acagacgtt gcctactct 
      cccgatgac aagtatcta gtggcctgt gatgcccac 
      agaaaagtc gtactgtat agtgttcct gaatacaag 
      ctcgccacc aatcaagaa tggggtttc cacaacgcg 
      cgtgttaac agcgttgct tggtcgccc gactctttg 
      ctagtcgct agtggtagt ttggacacg agtatcatt 
      atctggtct gttgaaaag cccgccaaa catattata 
      atcaaaaac gctcatgct caaagtcaa gtcactaga 
      ttagcctgg cttgacgac cagactttg gtctcagtg 
      gggcaggat tgcaacacg aaattgtgg accatcacc 
      aagttctaa
    mRNA sequence
    • >TC005722 RA
      aggttttgc gaaatttgt gaaataaaa ttatttgcc 
      aagtgttca taaccagtg cgtgtgcca agagaattt 
      ctcgtaaac tgccaacac gtagtgtaa acttttcta 
      ctacaatat ctccgtgtt ccacttgag gattcttac 
      catttccgg caaaccgga tcatttttc acataaaaa 
      aaacaggaa aatgtcatt tgcatcaaa gagtattta 
      cgcgacttt gccgcggac ccaacgagg acagcccct 
      ggtgttggg cggggatcc caaaggcaa gaactttct 
      ctacaccaa tggcaattc cgtgatcat tcgcaacat 
      ccaaaaccc tgcaattgc cgacgtcta cacggaaca 
      ttcctgtcc ggtaaacgt cgccaagta ctcccctag 
      cggcttcta catcgcctc tggagacca atcgggcaa 
      agtcagaat ctgggacac agtcaacaa ggagcacat 
      ccttaaaaa tgagtttca cccaatcgg ggggcccat 
      caaagatat cgcttggtc ccctgataa ccaacgaat 
      ggtcgtggt tggcgaagg ccgcgaacg cttcggtca 
      tgttttcat gtccgaaac gggaacttc cgttggcga 
      aatctcagg ccaatcaaa acccatcaa ttcctgcga 
      tttcaaacc cacgagacc tttcagaat catcacggg 
      gtctgaaga caacaccat tgcggtttt cgaggggcc 
      cccgtttaa gttcaaaat gaccaagca ggagcacag 
      caggtttgt gcaagccgt tcgttattc acccaccgg 
      taatttatt tgcctcggc tgggttcga tggtaaggt 
      gtttctgta cgatggtac gtcctccga tttggtggg 
      ggaagtggg aagccccgc ccacagcgg gggcgtgta 
      tggggttag ttggagtcc cgacggtaa acaattgct 
      aacagccag tggggacaa aactgcgaa attgtggga 
      cgtggaaac gagacaagt cgtctctga attcgtcct 
      aggttcgac agttgaaga tcaacaagt ttcgtgttt 
      atggcaaaa cgaatactt gctcacggt ttctttgag 
      tggttttat taattattt ggatattaa tgacccaac 
      gaagccgct ccgggttgt taaagggca caataaacc 
      catcactgt cttagaatt gagcgaaga tcgtggtac 
      gatctttac cgggtcgca tgatggtaa tgtgactcg 
      atggaacgc cgaaactgg ggagaacga tcgggttga 
      gggcgtggg tcatggtaa ccagatcaa tgggatgag 
      ggtacatgg tggtacttt gtacacttg tggtattga 
      tgactcttt aaaacaaat cgacattga gggcaacac 
      gtacaaagg gcaagattt gaagttggg gtcgcaacc 
      tcgcggaat ggacatttt aaaagagca aaatgtggt 
      ggtcacagc gagtgttaa cgaaatcac cgtctcgca 
      agataacag gaaagcaag cactttgaa ggtgtctta 
      tgaaccgtc aagtgtcag cgtttcgcc gcggggtca 
      cgtggctgt gggtggtgc catggacaa caaggtgca 
      tttatacga actgaaagg cacagagtt ggtaccctt 
      gcaagaatt ggaccattt gggtgccgt aacagacgt 
      tgcctactc tcccgatga caagtatct agtggcctg 
      tgatgccca cagaaaagt cgtactgta tagtgttcc 
      tgaatacaa gctcgccac caatcaaga atggggttt 
      ccacaacgc gcgtgttaa cagcgttgc ttggtcgcc 
      cgactcttt gctagtcgc tagtggtag tttggacac 
      gagtatcat tatctggtc tgttgaaaa gcccgccaa 
      acatattat aatcaaaaa cgctcatgc tcaaagtca 
      agtcactag attagcctg gcttgacga ccagacttt 
      ggtctcagt ggggcagga ttgcaacac gaaattgtg 
      gaccatcac caagttcta ataattttt ttaaatggc 
      ctttttgca tttaacgta aagcttttt tatttatta 
      catacagta gggtgatgc agtggcggg cctaaccga 
      cgtgtcaat taatttatt tcaagtatt gattttcta 
      atagtgtaa atatttaaa tcgaggagt gttatgatg 
      aatgaaaaa tctgctaaa tcatccaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY