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Gene information
General information
TC005718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 1465131..1472167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005718
    iBeetle screen iB_00911
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005718 RA
      MASNIPDLF YSCEICGLE GLSEDEFRT HTRTAHVDG 
      NAVCPFCEL SAVSPAELI LHVNQAHLD YLTPESESN 
      MTFIDDVSP SEYNGINGE SSDNWNIPS LPSHNKTNI 
      NNINLSPKV NGGGELSPK NSTHGQGSP LRSSLALKL 
      KSAAPRMQC PMCNYTSNS PNELEEHVN RRHFDVTSP 
      SMGGTTSEI YSCPLCVKS FNSAPDLEL HVNIEHRDV 
      LSPASPPTH SCPVCGMNL DNASNSESA ARHVESHFP 
      ATSPQPADR AALREREQR EFEMLRAQY GMDNQGNFR 
      EQSVTNMQR AVYAGEMSV ADYYERTLD LRAAESCGI 
      DDGSSITRS IVPRVRAIS TTAPNVVRT LLCTCVDHY 
      ASSYGDRGW GCGYRNMQM LISSLLTHT GYNERLYKL 
      WQGQKPPRS SVPSISRLQ SLIEQAWSQ GFDIQGSEQ 
      LGCRLVNTR KWIGATEVV TLLSFLRIK CQLVDFHRP 
      TGPGGTHPE LFTWVLKYF ENSVGGEFV PPLYLQHQG 
      HSRTIMGIE VHRDGSLIL LVLDPSHSP QQMAQFGDT 
      NSSAVALRL LRKSEAAMK ARQYQIVAV VGTIDSEQQ 
      YQQSKILRG TRIPQDR
    CDS sequence
    • >TC005718 RA
      atggctagc aacatcccc gatttgttc tactcctgc 
      gagatctgc ggcctggag ggcttgagt gaagacgag 
      ttccggacg cacacacgc actgcccac gtcgacggc 
      aatgccgtg tgccccttt tgcgagtta tcggccgtc 
      tcgcccgct gaactcatc cttcacgtc aaccaggcc 
      cacttggac tacctcacc cccgaatcc gagtccaac 
      atgaccttc atcgacgac gtcagcccc agcgagtac 
      aacggtatt aatggggaa agtagtgat aattggaac 
      attccgtct ttgcccagc cataataaa accaacatt 
      aacaacatc aacctgtcc ccgaaagtt aacgggggc 
      ggcgaactc agtccgaaa aattccacc cacggtcag 
      ggctccccc ttgcgatcc agtctcgcc ttgaagttg 
      aagagtgcg gcccccaga atgcagtgc cccatgtgc 
      aattatacg agcaacagt ccaaacgag ctcgaggag 
      cacgtcaac aggcggcat tttgacgtg acgtcgcct 
      tcgatgggg ggcaccacc agcgagata tactcgtgt 
      cccttatgc gtgaagtcg ttcaattcg gcccccgac 
      ttggagctc catgtcaac atcgagcac cgtgacgtg 
      ctgtcgcct gccagtccc ccaacacat tcgtgtccc 
      gtctgcggt atgaatcta gacaacgca agcaatagt 
      gagagcgct gctcgtcac gtggagagt cattttccg 
      gccacttcg cctcagccc gccgacaga gcagcgttg 
      cgtgaaagg gagcagcgg gaattcgaa atgttgagg 
      gcgcagtac ggcatggac aaccaggga aacttcagg 
      gaacagtcc gttacgaac atgcaaaga gctgtgtac 
      gcgggcgag atgagtgtt gccgattat tatgaacgt 
      acgttggat ttgagagcg gctgaaagt tgtgggatt 
      gacgatggg agctccata accaggagt attgtgcct 
      agagttcga gccattagc actactgca cctaatgtg 
      gtccgaact ttactctgt acctgcgtt gatcactac 
      gcttcatcg tatggtgat agaggctgg ggctgcgga 
      tacaggaat atgcagatg ttgatatca agtctgctt 
      acacacact ggatacaac gaacgctta tacaagcta 
      tggcagggc cagaagccg ccccgcagt tccgttccg 
      agcatatcc cgcctccaa agcctcatc gagcaggcc 
      tggtcccaa ggtttcgac attcagggt tcggagcaa 
      ttaggttgc cgtcttgtt aatactcgc aaatggatt 
      ggagccacc gaagtcgtc acactctta tcatttctc 
      cgaatcaaa tgccagctg gtagacttt catcggccg 
      acaggcccc ggcggcacc catccagaa ctcttcacc 
      tgggtgttg aaatatttc gaaaacagc gttggtggt 
      gagttcgtt ccaccactg tatttacaa catcaaggt 
      cacagccgg accatcatg ggcatcgag gtgcaccga 
      gacggcagc ttgatcctt ctagtgcta gatccgagc 
      cattccccc caacagatg gcgcagttt ggcgacacg 
      aacagctca gctgtggct ttgcgcctt ttgaggaaa 
      agcgaggcg gccatgaaa gcgcgccag taccagatt 
      gtggcagtg gttggaact atcgactcg gagcagcaa 
      tatcagcaa agtaaaatt ctgaggggt acgagaata 
      ccgcaggac cggtga
    mRNA sequence
    • >TC005718 RA
      acattttca aaattggac gaaaatcac aacgtaaac 
      agcaggatg gctagcaac atccccgat ttgttctac 
      tcctgcgag atctgcggc ctggagggc ttgagtgaa 
      gacgagttc cggacgcac acacgcact gcccacgtc 
      gacggcaat gccgtgtgc cccttttgc gagttatcg 
      gccgtctcg cccgctgaa ctcatcctt cacgtcaac 
      caggcccac ttggactac ctcaccccc gaatccgag 
      tccaacatg accttcatc gacgacgtc agccccagc 
      gagtacaac ggtattaat ggggaaagt agtgataat 
      tggaacatt ccgtctttg cccagccat aataaaacc 
      aacattaac aacatcaac ctgtccccg aaagttaac 
      gggggcggc gaactcagt ccgaaaaat tccacccac 
      ggtcagggc tcccccttg cgatccagt ctcgccttg 
      aagttgaag agtgcggcc cccagaatg cagtgcccc 
      atgtgcaat tatacgagc aacagtcca aacgagctc 
      gaggagcac gtcaacagg cggcatttt gacgtgacg 
      tcgccttcg atggggggc accaccagc gagatatac 
      tcgtgtccc ttatgcgtg aagtcgttc aattcggcc 
      cccgacttg gagctccat gtcaacatc gagcaccgt 
      gacgtgctg tcgcctgcc agtccccca acacattcg 
      tgtcccgtc tgcggtatg aatctagac aacgcaagc 
      aatagtgag agcgctgct cgtcacgtg gagagtcat 
      tttccggcc acttcgcct cagcccgcc gacagagca 
      gcgttgcgt gaaagggag cagcgggaa ttcgaaatg 
      ttgagggcg cagtacggc atggacaac cagggaaac 
      ttcagggaa cagtccgtt acgaacatg caaagagct 
      gtgtacgcg ggcgagatg agtgttgcc gattattat 
      gaacgtacg ttggatttg agagcggct gaaagttgt 
      gggattgac gatgggagc tccataacc aggagtatt 
      gtgcctaga gttcgagcc attagcact actgcacct 
      aatgtggtc cgaacttta ctctgtacc tgcgttgat 
      cactacgct tcatcgtat ggtgataga ggctggggc 
      tgcggatac aggaatatg cagatgttg atatcaagt 
      ctgcttaca cacactgga tacaacgaa cgcttatac 
      aagctatgg cagggccag aagccgccc cgcagttcc 
      gttccgagc atatcccgc ctccaaagc ctcatcgag 
      caggcctgg tcccaaggt ttcgacatt cagggttcg 
      gagcaatta ggttgccgt cttgttaat actcgcaaa 
      tggattgga gccaccgaa gtcgtcaca ctcttatca 
      tttctccga atcaaatgc cagctggta gactttcat 
      cggccgaca ggccccggc ggcacccat ccagaactc 
      ttcacctgg gtgttgaaa tatttcgaa aacagcgtt 
      ggtggtgag ttcgttcca ccactgtat ttacaacat 
      caaggtcac agccggacc atcatgggc atcgaggtg 
      caccgagac ggcagcttg atccttcta gtgctagat 
      ccgagccat tccccccaa cagatggcg cagtttggc 
      gacacgaac agctcagct gtggctttg cgccttttg 
      aggaaaagc gaggcggcc atgaaagcg cgccagtac 
      cagattgtg gcagtggtt ggaactatc gactcggag 
      cagcaatat cagcaaagt aaaattctg aggggtacg 
      agaataccg caggaccgg tgaggttac cccgtctcg 
      tgacctaaa attatgaat atctacttt tcaccacct 
      ctgctgttc atatattac taaagggaa ttttggagt 
      gtgccctgg ccacgctgc tatttattt ttcaatcat 
      catggcctc gaaacgtca ataaagtag atagaatga 
      gcttagatt tgattttta gttggggaa tttcgttcc 
      cttagatcg agtgattct tgaacaata ttaaaaaat 
      tacgacaaa agtgttaat actgttaca aaataacta 
      tttttgacc aaaagatga agtatcatt ttagtcaat 
      ttattcaag tacactgtt gtaggattt ttgtcctta 
      aaacgcttg ttactatta tttgtgata cgatattta 
      agtttttta tttattact agtcgtagg tccattttg 
      tcactattg aattttacc tctattaca cgaatgtac 
      aaatcgatt taaaaaatc atggtttat taaagattc 
      actgctgtg