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Gene information
General information
TC005702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 1769437..1773167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005702
    iBeetle screen iB_00910
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005702 RA
      MNHPIMFPG PLRRCKRVL PVILFFVSI VIIIVYRLS 
      VKKSEIHPK HIYRANNQE EYVDKNGVK VVVGHYIGN 
      PVHAIPNAT HDMINENNY DPRPGAGKN GNPVVIEPK 
      DLLKMQQYF QINRFNLLA SDRIPLNRS LPDFRRKKC 
      ATLFGDYPT YPKTSIIIV FHNEAWSTL LRTVWSVIN 
      RSPPELLEE IILVDDSSE RKFLKKPLD DYVANLPVP 
      TKVLRSQAR IGLIKARLK GALVAKGPV LTFLDAHCE 
      CTTGWLEAL LSVIKQDRT AVVCPVIDI INDDTFAYV 
      KSFELHWGA FNWNLQFRW FTLGGRELK LRKNDATQP 
      FNTPTMAGG LFAIDREYF FEMGAYDDG MNIWGGENL 
      EMSFRIWQC GGKVQIAPC SRVGHLFRK SSPYSFPGG 
      INKTLFSNL ARVARVWMD DWARFYFKF NEPADRIKN 
      EQNVTSRIE LRRKHKCKG FEWYLDNVW PQHFFPKDD 
      RFFGRIRNL GQNMCLIKP QKKVVSNQP MGIAKIDMC 
      LGDEVILEM FVMTKEGFI MTDDSICLD APEKVVIGP 
      SKVRIMACS GYSRQKWVY DKEKQEIRH LTNQKCLDV 
      SDSSKFTDG LITTNCTGS LTQKWALES VPWK
    CDS sequence
    • >TC005702 RA
      atgaatcat ccgattatg tttcctggc cctttacga 
      agatgtaaa cgagttttg cctgtgatc ctctttttt 
      gtgtccatt gtgataata attgtctat agattaagc 
      gtgaaaaag agcgaaata catccaaaa cacatctac 
      agggccaac aatcaggaa gaatatgtt gataaaaat 
      ggtgttaag gtagttgtg ggtcattat ataggaaat 
      cccgttcat gcgatcccc aatgcgacc cacgacatg 
      attaatgaa aataattac gaccctaga ccaggggcc 
      ggaaaaaac ggcaatccg gttgtaatc gaaccgaaa 
      gacttatta aaaatgcag caatacttc caaataaat 
      cggttcaac cttttggct agtgacaga atccctctg 
      aatcggtct ttacccgat tttcgtcga aaaaaatgc 
      gcaacgtta tttggcgac tatccgact tatccaaaa 
      accagtatt attatagtt ttccacaac gaggcctgg 
      tccactctg ctgcggacc gtctggagt gtgataaac 
      cgaagtccg cccgaactt ttggaggaa attatccta 
      gtggacgat tcaagcgaa cgaaaattt ctcaaaaaa 
      cctctggac gactacgtg gccaatctt cccgttccg 
      acgaaagtc ctccgtagc caggcccgg atcggtttg 
      atcaaagca agactgaag ggggccctc gtggctaaa 
      ggccccgtt ttaaccttt ctggacgcc cactgcgag 
      tgcaccacc ggttggtta gaagccctc ttatctgtg 
      ataaaacag gacaggact gcggtcgtc tgccccgtc 
      atcgacata ataaacgac gacacgttc gcctacgtc 
      aaaagcttc gaactgcac tggggggcc ttcaactgg 
      aacttgcag ttccggtgg ttcaccctc ggcgggcgc 
      gaactcaaa ctacgaaaa aacgacgcc actcagccc 
      ttcaacacc cccacgatg gcagggggg ctgtttgcc 
      atcgaccgg gagtacttt ttcgaaatg ggggcgtac 
      gacgacggg atgaacatc tggggcggg gagaatctg 
      gaaatgtcg ttcaggatc tggcaatgt gggggcaag 
      gtccagatc gccccctgt tcaagggtg gggcacttg 
      tttcggaaa tcgtcgcct tactccttt ccagggggg 
      attaacaag acgcttttc tcgaacctg gcacgagta 
      gcaagggtt tggatggac gattgggcg agattttat 
      ttcaagttc aacgaacct gcagacagg attaaaaac 
      gagcaaaat gtgacttcg cgaattgaa ttaaggagg 
      aaacataag tgtaaaggt tttgaatgg tatttggat 
      aatgtttgg ccgcaacat ttcttccca aaggatgat 
      aggtttttc ggccggatt cgaaatttg ggccagaat 
      atgtgtctg attaaacca caaaagaaa gtggtctcg 
      aaccaacca atgggcatt gctaagatc gatatgtgt 
      cttggtgat gaggttatt ttggaaatg tttgttatg 
      accaaagaa ggttttatc atgactgac gactcgatt 
      tgtttggac gcacccgaa aaagttgtg atagggccg 
      tccaaggtc cgaattatg gcctgtagt gggtacagc 
      aggcaaaaa tgggtctac gacaaggag aagcaggaa 
      atccgccat ttgaccaac caaaagtgc ctggacgtg 
      tctgactcc tcgaaattc actgacggt ttaataacg 
      accaactgc actggtagt ctgacccaa aaatgggct 
      ttggagagt gtcccttgg aagtaa
    mRNA sequence
    • >TC005702 RA
      atgaatcat ccgattatg tttcctggc cctttacga 
      agatgtaaa cgagttttg cctgtgatc ctctttttt 
      gtgtccatt gtgataata attgtctat agattaagc 
      gtgaaaaag agcgaaata catccaaaa cacatctac 
      agggccaac aatcaggaa gaatatgtt gataaaaat 
      ggtgttaag gtagttgtg ggtcattat ataggaaat 
      cccgttcat gcgatcccc aatgcgacc cacgacatg 
      attaatgaa aataattac gaccctaga ccaggggcc 
      ggaaaaaac ggcaatccg gttgtaatc gaaccgaaa 
      gacttatta aaaatgcag caatacttc caaataaat 
      cggttcaac cttttggct agtgacaga atccctctg 
      aatcggtct ttacccgat tttcgtcga aaaaaatgc 
      gcaacgtta tttggcgac tatccgact tatccaaaa 
      accagtatt attatagtt ttccacaac gaggcctgg 
      tccactctg ctgcggacc gtctggagt gtgataaac 
      cgaagtccg cccgaactt ttggaggaa attatccta 
      gtggacgat tcaagcgaa cgaaaattt ctcaaaaaa 
      cctctggac gactacgtg gccaatctt cccgttccg 
      acgaaagtc ctccgtagc caggcccgg atcggtttg 
      atcaaagca agactgaag ggggccctc gtggctaaa 
      ggccccgtt ttaaccttt ctggacgcc cactgcgag 
      tgcaccacc ggttggtta gaagccctc ttatctgtg 
      ataaaacag gacaggact gcggtcgtc tgccccgtc 
      atcgacata ataaacgac gacacgttc gcctacgtc 
      aaaagcttc gaactgcac tggggggcc ttcaactgg 
      aacttgcag ttccggtgg ttcaccctc ggcgggcgc 
      gaactcaaa ctacgaaaa aacgacgcc actcagccc 
      ttcaacacc cccacgatg gcagggggg ctgtttgcc 
      atcgaccgg gagtacttt ttcgaaatg ggggcgtac 
      gacgacggg atgaacatc tggggcggg gagaatctg 
      gaaatgtcg ttcaggatc tggcaatgt gggggcaag 
      gtccagatc gccccctgt tcaagggtg gggcacttg 
      tttcggaaa tcgtcgcct tactccttt ccagggggg 
      attaacaag acgcttttc tcgaacctg gcacgagta 
      gcaagggtt tggatggac gattgggcg agattttat 
      ttcaagttc aacgaacct gcagacagg attaaaaac 
      gagcaaaat gtgacttcg cgaattgaa ttaaggagg 
      aaacataag tgtaaaggt tttgaatgg tatttggat 
      aatgtttgg ccgcaacat ttcttccca aaggatgat 
      aggtttttc ggccggatt cgaaatttg ggccagaat 
      atgtgtctg attaaacca caaaagaaa gtggtctcg 
      aaccaacca atgggcatt gctaagatc gatatgtgt 
      cttggtgat gaggttatt ttggaaatg tttgttatg 
      accaaagaa ggttttatc atgactgac gactcgatt 
      tgtttggac gcacccgaa aaagttgtg atagggccg 
      tccaaggtc cgaattatg gcctgtagt gggtacagc 
      aggcaaaaa tgggtctac gacaaggag aagcaggaa 
      atccgccat ttgaccaac caaaagtgc ctggacgtg 
      tctgactcc tcgaaattc actgacggt ttaataacg 
      accaactgc actggtagt ctgacccaa aaatgggct 
      ttggagagt gtcccttgg aagtaa