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Gene information
General information
TC005697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 1829089..1833813
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005697
    iBeetle screen iB_00909
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005697 RA
      MSSKRDRMG NRKEGNHER PPSHLRGKQ IGMYYAQRN 
      RERNEANRK RPLGTILLS ESRKNQIQA VLNSTSFKN 
      IFATTSSTY EHLEDSAFK RSFLRVINE TIDEKLQHT 
      CARLAEDAT LNQSLYEDF LQKQSSPKY MNMIAKRTK 
      LPAFNMKDE ILKVIDENQ VVVISGETG CGKTTQVAQ 
      FILDDFLQK QKGSVCKVL CTQPRRISA IAVAQRVAE 
      ERGEELGHS VGYHIRMER RPPRDRGSI CFCTTGVVL 
      KIMESDASL SWVSHLILD EIHERDVMS DFILALIKK 
      IKAKRSDLK IILMSATLN SEKFSKYYD NAPHLNIPG 
      FTYPVQEFY LEDVLQRTG FVFESTHRI KHKKTKMYS 
      DFIEPHVRQ LERTRQYSR QVCIQLRNP ECEDINLEL 
      ILQLVIDVC GKERDEGAI LIFLTGFHE ISTLSRLMS 
      ESGRFPPGK FLIFPLHSL MPTLEQKQI FDTPPRGMR 
      KIIIATNIA ETSITIDDV VYVIDCGKI KVTNFDART 
      NSDILAPEW VSLANANQR RGRAGRVKP GMCFHLFTK 
      ARNMVLEQY LLPEILRKR LEDVILTAK ILQLGPVEP 
      FFAQLIDSP DPGAVTVAL ELLKRMNAL TDDEKLTPL 
      GYHLAKLPM APQIGKMIL FGAIFSCLD PILSIAAAL 
      DFKDPFQLP VDKEREVYK MKLELARGV KSDHLLFHE 
      ALRGFEESG NARQYCWNY FLSHQTMKQ LQDLKKQYM 
      EYLCEMNFV RDSDPKNPE CNLNWDNLS LVKAIICAG 
      LYPNISISP VLGKAPVRT PSMRRLKLH PKSILAECK 
      YFDTNILVY YKKMKSKVD FIYDASLIH PLPVIFFGD 
      RFNQICEDG RSFISINQN LRFKCSEST ASIIKELRD 
      RFNWFLEYK ISHPGPVVW GGDDETQIL SLIMELITC 
      EDMEEFDDD AFDDE
    CDS sequence
    • >TC005697 RA
      atgtcgagt aaaagagac cgaatgggc aatagaaaa 
      gagggtaat catgagaga ccaccttcg cacttgcga 
      ggtaaacaa atcggcatg tattatgcc caaagaaac 
      agagaaagg aatgaagcg aacaggaaa cgaccattg 
      gggaccatc ttgttatca gaatctcgc aagaatcaa 
      atccaagca gtattaaat tccacatcg ttcaaaaat 
      atttttgct actaccagc agcacgtac gagcatctt 
      gaagactca gctttcaaa cggagcttt cttcgagtt 
      attaacgag accattgac gaaaagttg caacacacg 
      tgcgcacgt ctcgccgaa gatgcaact ttgaatcaa 
      tcactgtat gaagatttt ttgcaaaaa cagtctagt 
      cctaaatac atgaacatg atagcaaaa cggacgaag 
      ctgccggcg tttaatatg aaagacgag atcttgaaa 
      gtcattgac gagaatcaa gttgttgtg atcagtggc 
      gagactggg tgcggaaaa acgacccaa gttgctcag 
      tttatcctt gatgatttt ttgcaaaag cagaaaggc 
      tcagtgtgt aaggtttta tgcacacaa ccacgcaga 
      attagcgct attgccgtg gcacagagg gtggccgag 
      gaacgcggt gaggagctg ggccacagc gtggggtac 
      catatcagg atggaaaga cgtccgcct cgcgatcgg 
      ggctccatc tgtttttgc acaactggc gtggttcta 
      aaaatcatg gaatcggac gcttcgctc tcttgggtc 
      tcccacttg atccttgac gaaatccac gaacgtgac 
      gtaatgtcc gacttcatc ctagcactc attaaaaaa 
      atcaaagcg aaacgaagc gacttgaaa atcattttg 
      atgagcgcc actctcaat tcggaaaaa ttctcaaag 
      tattacgac aacgccccg catctaaac atccccggg 
      ttcacctac cccgtccag gaattctac ctcgaagac 
      gtcctacaa cgcaccggc ttcgtcttc gagtccacg 
      caccgcatc aaacacaaa aaaaccaaa atgtacagc 
      gacttcatc gagccacat gtccgccag ctagagcgc 
      acgagacag tacagtcgc caggtctgc atccagctg 
      cgcaacccc gaatgcgag gacatcaac ctggagctg 
      atcctccag ctcgtcatc gacgtctgt gggaaggag 
      cgggacgaa ggcgcgatt ttgattttc ctgaccggc 
      ttccacgaa atctccact ttgagccgc cttatgtcg 
      gaaagtggg cgtttcccg cccggcaaa ttcctcatt 
      ttcccccta cactcgctc atgccgacg ctggaacaa 
      aagcagatc ttcgacacg ccccctcgc ggcatgcga 
      aaaatcatc atcgccacg aacattgcg gagacatcg 
      atcacaatc gacgacgtt gtttatgtg atcgactgc 
      ggaaaaatc aaagttacg aacttcgac gcccgaaca 
      aactcggac attttagcc cccgagtgg gtgtcgtta 
      gccaatgcc aaccaacgt agagggcgc gcaggccgc 
      gtcaagccc ggcatgtgc ttccatctc ttcacaaag 
      gcccggaac atggttctg gagcaatac ctcctgcct 
      gagatttta cgcaaacgg ctggaggat gtgattttg 
      accgcgaaa atcctacag ttgggccca gtcgagccg 
      tttttcgcc cagttaatc gactcaccg gaccccgga 
      gcggtcacg gtagcccta gagttgcta aagcgcatg 
      aacgcgtta accgacgac gagaaatta accccgttg 
      gggtaccac ttggccaag ttgcccatg gcgccacag 
      atcggcaaa atgatcctt ttcggcgcc attttcagc 
      tgcttggac cccattttg tcgatcgcc gcggcgctc 
      gatttcaaa gacccgttc cagttgccg gtggataaa 
      gaaagggaa gtgtacaaa atgaaactg gaactggca 
      cggggcgtg aaaagtgac cacttgttg tttcacgaa 
      gctttgcgg gggtttgag gagagcgga aacgcgcgg 
      cagtactgc tggaactac ttcctgagt caccagacc 
      atgaagcaa ctgcaggac ttgaagaaa cagtacatg 
      gagtatttg tgcgagatg aacttcgtg cgagattcg 
      gacccgaaa aaccctgag tgtaatttg aattgggat 
      aatctgagc ctggttaag gccatcatt tgcgctggg 
      ttgtacccg aacattagc atttcgcct gttttaggg 
      aaggcgccc gtgaggacg cccagtatg aggcggttg 
      aagcttcat ccgaaatca attttggcc gagtgtaaa 
      tacttcgat acaaacatc ctcgtttat tataagaaa 
      atgaaatcg aaagtggat tttatttac gacgcgtca 
      ttaattcac ccgttacct gtgatattt ttcggcgat 
      aggtttaac cagatttgt gaggatggg cgcagtttc 
      atttccatt aaccagaat ttgaggttt aagtgttcg 
      gaaagcacg gcttcgatt attaaggaa ctgagagat 
      cggttcaat tggtttttg gaatacaaa atatcacac 
      ccggggcca gtggtgtgg ggaggagac gatgagacg 
      caaatttta agtcttata atggaactg attacttgc 
      gaagacatg gaagagttc gatgatgat gcgtttgat 
      gacgaatga
    mRNA sequence
    • >TC005697 RA
      cacggcggc gagctttcc cccatgtca gtcattaca 
      taaccccac tttttgctc aaaattttt tgttttcaa 
      caaaaaaaa tgtcgagta aaagagacc gaatgggca 
      atagaaaag agggtaatc atgagagac caccttcgc 
      acttgcgag gtaaacaaa tcggcatgt attatgccc 
      aaagaaaca gagaaagga atgaagcga acaggaaac 
      gaccattgg ggaccatct tgttatcag aatctcgca 
      agaatcaaa tccaagcag tattaaatt ccacatcgt 
      tcaaaaata tttttgcta ctaccagca gcacgtacg 
      agcatcttg aagactcag ctttcaaac ggagctttc 
      ttcgagtta ttaacgaga ccattgacg aaaagttgc 
      aacacacgt gcgcacgtc tcgccgaag atgcaactt 
      tgaatcaat cactgtatg aagattttt tgcaaaaac 
      agtctagtc ctaaataca tgaacatga tagcaaaac 
      ggacgaagc tgccggcgt ttaatatga aagacgaga 
      tcttgaaag tcattgacg agaatcaag ttgttgtga 
      tcagtggcg agactgggt gcggaaaaa cgacccaag 
      ttgctcagt ttatccttg atgattttt tgcaaaagc 
      agaaaggct cagtgtgta aggttttat gcacacaac 
      cacgcagaa ttagcgcta ttgccgtgg cacagaggg 
      tggccgagg aacgcggtg aggagctgg gccacagcg 
      tggggtacc atatcagga tggaaagac gtccgcctc 
      gcgatcggg gctccatct gtttttgca caactggcg 
      tggttctaa aaatcatgg aatcggacg cttcgctct 
      cttgggtct cccacttga tccttgacg aaatccacg 
      aacgtgacg taatgtccg acttcatcc tagcactca 
      ttaaaaaaa tcaaagcga aacgaagcg acttgaaaa 
      tcattttga tgagcgcca ctctcaatt cggaaaaat 
      tctcaaagt attacgaca acgccccgc atctaaaca 
      tccccgggt tcacctacc ccgtccagg aattctacc 
      tcgaagacg tcctacaac gcaccggct tcgtcttcg 
      agtccacgc accgcatca aacacaaaa aaaccaaaa 
      tgtacagcg acttcatcg agccacatg tccgccagc 
      tagagcgca cgagacagt acagtcgcc aggtctgca 
      tccagctgc gcaaccccg aatgcgagg acatcaacc 
      tggagctga tcctccagc tcgtcatcg acgtctgtg 
      ggaaggagc gggacgaag gcgcgattt tgattttcc 
      tgaccggct tccacgaaa tctccactt tgagccgcc 
      ttatgtcgg aaagtgggc gtttcccgc ccggcaaat 
      tcctcattt tccccctac actcgctca tgccgacgc 
      tggaacaaa agcagatct tcgacacgc cccctcgcg 
      gcatgcgaa aaatcatca tcgccacga acattgcgg 
      agacatcga tcacaatcg acgacgttg tttatgtga 
      tcgactgcg gaaaaatca aagttacga acttcgacg 
      cccgaacaa actcggaca ttttagccc ccgagtggg 
      tgtcgttag ccaatgcca accaacgta gagggcgcg 
      caggccgcg tcaagcccg gcatgtgct tccatctct 
      tcacaaagg cccggaaca tggttctgg agcaatacc 
      tcctgcctg agattttac gcaaacggc tggaggatg 
      tgattttga ccgcgaaaa tcctacagt tgggcccag 
      tcgagccgt ttttcgccc agttaatcg actcaccgg 
      accccggag cggtcacgg tagccctag agttgctaa 
      agcgcatga acgcgttaa ccgacgacg agaaattaa 
      ccccgttgg ggtaccact tggccaagt tgcccatgg 
      cgccacaga tcggcaaaa tgatccttt tcggcgcca 
      ttttcagct gcttggacc ccattttgt cgatcgccg 
      cggcgctcg atttcaaag acccgttcc agttgccgg 
      tggataaag aaagggaag tgtacaaaa tgaaactgg 
      aactggcac ggggcgtga aaagtgacc acttgttgt 
      ttcacgaag ctttgcggg ggtttgagg agagcggaa 
      acgcgcggc agtactgct ggaactact tcctgagtc 
      accagacca tgaagcaac tgcaggact tgaagaaac 
      agtacatgg agtatttgt gcgagatga acttcgtgc 
      gagattcgg acccgaaaa accctgagt gtaatttga 
      attgggata atctgagcc tggttaagg ccatcattt 
      gcgctgggt tgtacccga acattagca tttcgcctg 
      ttttaggga aggcgcccg tgaggacgc ccagtatga 
      ggcggttga agcttcatc cgaaatcaa ttttggccg 
      agtgtaaat acttcgata caaacatcc tcgtttatt 
      ataagaaaa tgaaatcga aagtggatt ttatttacg 
      acgcgtcat taattcacc cgttacctg tgatatttt 
      tcggcgata ggtttaacc agatttgtg aggatgggc 
      gcagtttca tttccatta accagaatt tgaggttta 
      agtgttcgg aaagcacgg cttcgatta ttaaggaac 
      tgagagatc ggttcaatt ggtttttgg aatacaaaa 
      tatcacacc cggggccag tggtgtggg gaggagacg 
      atgagacgc aaattttaa gtcttataa tggaactga 
      ttacttgcg aagacatgg aagagttcg atgatgatg 
      cgtttgatg acgaatgaa aacatgttt ccaattttg 
      taaaaaaat gttttggtt aataaatta ataattcag 
      at