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Gene information
General information
TC005682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9733107..9735894
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005682
    iBeetle screen iB_00908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment posterior dorsalbent - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005682 RA
      MEPMQGNSP ESVTTCGTD DERCCLTHQ PSCHSSKSL 
      LTEEKMRRK LQFFFMNPI EKWQAKKRF PYKFTVQLI 
      KIILVTMQL CLFAYNRYN HVNYTWDNR ISFSHLFLR 
      GWDATREVS AYPPATGPL AIYKADDFY ATIDYAYSG 
      YANLSQAVG SYSYANEDN TMTDMELCL SQYKKGIIF 
      GFNESYVFD AEIVDTCLN ITHRQDHLL DSKQYLDNL 
      NINFSALVK AVLKFSVKT VNFKTAGRI SPPNCYRFD 
      ININFDNED HDGQMLLWL DAQPVRLMC KGDVEYVTD 
      DEIDSLLQS LLNYLVITI CILSFVLCT RALLRAQKL 
      KKMTNSFFI NQFGRPLSK EDRNKFLNL WYVMIIIND 
      ILIIIGSSI KEQIERKDF TSDQWNVCS LFLGLGNML 
      VWFGVLRYL TFFKTYNVV ILTLEKAAP QVARFLLCA 
      LLIYAGFTF SGWLILGPY HLKFRSLST TSECLFSLI 
      NGDDMFATF SIMSSKSTM LWWFSKIYL YSFISLYIY 
      IILSLFISV IMDAYDTIK IYYIEGFPK SDLQIFIGD 
      TNFEDVSSG IFRTASNDS LGGLMKDLC CCTCTKLQK 
      SYSSLSRGS LATEGSRRG SNAV
    CDS sequence
    • >TC005682 RA
      atggagcca atgcaaggc aattcccca gaaagcgtc 
      acaacctgt ggcactgac gacgaacgg tgttgtctc 
      acccaccag ccctcctgt cacagctcc aagtcgctc 
      ctaaccgag gaaaaaatg cgtcgcaag ctccagttc 
      ttcttcatg aaccccatc gagaaatgg caagccaag 
      aaacgcttc ccttacaaa ttcacagtg cagctaatc 
      aagataatc ctcgtgaca atgcagctg tgtctcttc 
      gcctacaat cgctacaac cacgttaat tacacgtgg 
      gacaatcgg atttcattc tcgcacttg tttctacgc 
      ggctgggat gccacacgt gaagtgagc gcctatcca 
      cctgccacg ggacctttg gccatctac aaagcggac 
      gatttttac gccactatt gactacgct tattcaggc 
      tatgccaat ttgagccaa gctgttggt tcgtactct 
      tacgccaat gaggacaat actatgact gatatggaa 
      ctgtgcctg tcacagtac aagaaaggc attattttt 
      gggttcaac gaatcgtat gtttttgac gccgaaatc 
      gtagacacc tgtcttaat ataacgcac aggcaggac 
      catctcctc gactctaaa caataccta gataacttg 
      aacataaac ttctcggct ttggtcaaa gccgtgctg 
      aagttctcg gttaaaact gttaatttt aaaacagcc 
      ggtcggatc tcgccaccg aattgctac cgctttgat 
      attaacatt aacttcgat aatgaggac cacgatggt 
      cagatgttg ctgtggttg gacgcacag ccggtccgt 
      ctcatgtgc aagggtgac gttgagtac gtcactgat 
      gacgaaatt gactcgctt ttgcaaagt cttttgaac 
      tatttggtt atcaccatt tgtattttg tcgtttgtt 
      ttgtgcaca agggccctt ctcagggcc caaaagttg 
      aagaaaatg accaattcg ttttttatc aatcagttc 
      ggacgccct cttagcaaa gaggatagg aacaagttt 
      ttgaatctg tggtatgtg atgataata atcaacgac 
      atcctcata atcatcggg tcgtcgatc aaggaacag 
      atcgaacgt aaggacttt accagcgat cagtggaac 
      gtctgtagc ttgttcctg gggcttggg aacatgctg 
      gtctggttt ggggttttg cgctacttg acgttcttt 
      aaaacgtac aatgttgtg attctgact ttggagaag 
      gcggcgcct caagtcgcc agattcctc ctttgtgcg 
      ttacttatt tacgcgggt tttaccttc tcagggtgg 
      ctgatcctc ggtccgtac catttgaag ttcaggtcg 
      ttgtccacg acgtcagag tgccttttc tctctgatt 
      aacggcgac gatatgttc gccactttc tccataatg 
      tcgagcaaa tcgaccatg ctgtggtgg ttcagcaag 
      atctatcta tactcgttt ataagcttg tacatttac 
      attattcta agtttgttt atttccgtc atcatggac 
      gcctacgat acgatcaag atctactac attgaggga 
      ttccccaag agtgattta caaattttc atcggcgat 
      accaacttt gaggatgta tcgagtggg atttttaga 
      accgcgtcc aatgatagt ttgggtggg cttatgaaa 
      gatctatgc tgttgcact tgtactaaa ctacaaaag 
      tcttattcg agtttgtca cgtggtagt ttagccact 
      gagggatca agacgaggt tcgaatgcc gtctaa
    mRNA sequence
    • >TC005682 RA
      aattaaact gtcccaaaa aaactagga aaaaacgaa 
      actccctag ttgcgaaac gtcagatgt cagtgtcaa 
      caattaggt tattttcct ttgtcagtg tttgtggtt 
      tggccccat caccaagtt aaaagtgaa tgtcctgtt 
      gagtgccgt ggaaatgga gccaatgca aggcaattc 
      cccagaaag cgtcacaac ctgtggcac tgacgacga 
      acggtgttg tctcaccca ccagccctc ctgtcacag 
      ctccaagtc gctcctaac cgaggaaaa aatgcgtcg 
      caagctcca gttcttctt catgaaccc catcgagaa 
      atggcaagc caagaaacg cttccctta caaattcac 
      agtgcagct aatcaagat aatcctcgt gacaatgca 
      gctgtgtct cttcgccta caatcgcta caaccacgt 
      taattacac gtgggacaa tcggatttc attctcgca 
      cttgtttct acgcggctg ggatgccac acgtgaagt 
      gagcgccta tccacctgc cacgggacc tttggccat 
      ctacaaagc ggacgattt ttacgccac tattgacta 
      cgcttattc aggctatgc caatttgag ccaagctgt 
      tggttcgta ctcttacgc caatgagga caatactat 
      gactgatat ggaactgtg cctgtcaca gtacaagaa 
      aggcattat ttttgggtt caacgaatc gtatgtttt 
      tgacgccga aatcgtaga cacctgtct taatataac 
      gcacaggca ggaccatct cctcgactc taaacaata 
      cctagataa cttgaacat aaacttctc ggctttggt 
      caaagccgt gctgaagtt ctcggttaa aactgttaa 
      ttttaaaac agccggtcg gatctcgcc accgaattg 
      ctaccgctt tgatattaa cattaactt cgataatga 
      ggaccacga tggtcagat gttgctgtg gttggacgc 
      acagccggt ccgtctcat gtgcaaggg tgacgttga 
      gtacgtcac tgatgacga aattgactc gcttttgca 
      aagtctttt gaactattt ggttatcac catttgtat 
      tttgtcgtt tgttttgtg cacaagggc ccttctcag 
      ggcccaaaa gttgaagaa aatgaccaa ttcgttttt 
      tatcaatca gttcggacg ccctcttag caaagagga 
      taggaacaa gtttttgaa tctgtggta tgtgatgat 
      aataatcaa cgacatcct cataatcat cgggtcgtc 
      gatcaagga acagatcga acgtaagga ctttaccag 
      cgatcagtg gaacgtctg tagcttgtt cctggggct 
      tgggaacat gctggtctg gtttggggt tttgcgcta 
      cttgacgtt ctttaaaac gtacaatgt tgtgattct 
      gactttgga gaaggcggc gcctcaagt cgccagatt 
      cctcctttg tgcgttact tatttacgc gggttttac 
      cttctcagg gtggctgat cctcggtcc gtaccattt 
      gaagttcag gtcgttgtc cacgacgtc agagtgcct 
      tttctctct gattaacgg cgacgatat gttcgccac 
      tttctccat aatgtcgag caaatcgac catgctgtg 
      gtggttcag caagatcta tctatactc gtttataag 
      cttgtacat ttacattat tctaagttt gtttatttc 
      cgtcatcat ggacgccta cgatacgat caagatcta 
      ctacattga gggattccc caagagtga tttacaaat 
      tttcatcgg cgataccaa ctttgagga tgtatcgag 
      tgggatttt tagaaccgc gtccaatga tagtttggg 
      tgggcttat gaaagatct atgctgttg cacttgtac 
      taaactaca aaagtctta ttcgagttt gtcacgtgg 
      tagtttagc cactgaggg atcaagacg aggttcgaa 
      tgccgtcta agaatagaa aaaatacag ttttgtaaa 
      tattttatt gtatataga atagaatat acgttttgt 
      acagaaaca tttatataa atatactga ctagatcaa 
      aatatgttc gagataagc tttaaatgt atctctgtc 
      tcaataaaa tttatttat gtataatct gagtttcac 
      tgcaaacct aaatcccca aagaatctt gtttccgat 
      tcaagctaa tcggctgcg acaatgttg gtcaataag 
      taaactaat taatactat caactgacg tcacagttt 
      cttcattca aaaaatata gaaaggaac aatcgcaac 
      gtgcttttt agtgttgca tataaatgc tgtatcaat 
      aagtaatta attaccctg gcttattca tttaatatt 
      atcagcctc cattacacg aaaatattt atttaaata 
      agtaactgt tcgtttcag cactaataa cactgtcca 
      acacttaaa atttaaatc gttgttaaa aattaacgc 
      gattggacc aatcacatt tgctcaatt tggtcatgt 
      gatcagtta atcgtgaat taaattaat gatggttct 
      ataaactgg ttgtaactt gaaacgatt ttcagaaaa 
      tcgcttttc tttgcttcc tgttaaagt aattaatgg 
      taaaaattg tatgtttat ttatatcca aaagacaaa 
      aataacctc ataattaca tgtagtaaa tagtacaaa 
      agttaacac gaacaaaat aattttaaa taagaacat 
      aaaatcata gaaaataaa aataaagat catggctta 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY