| General information | |||
| TC005672 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9822341..9831840 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005672 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00906 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
- >TC005672 RA
MPPRLKHIE VENFKSYKG HRIIGPLKP FNAVIGPNG SGKSNFMDA ISFVMGEKT QSLRVKRLS DLIHGAAIS KPISRSASV AAVFVLDEE SGKEICFQR SVQGSSSEY RINGTVVSN NEYLTELEK LRINVKGKN FLVFQGAVE SVAMKNPKE MTALFEEIS GSGALKEEY DRLKQQMQK AQEEINFAY QKKKGINAE RKEARLEKE EADKYSRLK DDLNDKLVE HQLFRLYHN EREMKNLEN DLKHKQREV EKIEKKKEK AEEVLKEKK KEQGKFNRE LAKIEQDIR EVEVEISKK RPQFIKAKE RVSHMQKKL DGAIKTLEQ ARKAHEAHM NDIKKLEDE LAEVEKTKD EYESQIAGE SQSQGRDVH LEDEQVREY HRLKEEAAK RSARYMQEL DSVNREQKS DQDRLDNVS RMRTDAENK HRQKCHEKE EMEKRIEKL AEHIRLSEQ ALQDQKQLR SDLQSDVGS SKDRVHEIQ KQLDDVLEQ LGDARTDKH EDARRKKKQ EIVERFKSN YPGVYDRMI NMCQPIHKR YNVAITKVL GKFMEAIVV DSEHTARQC IKYLKEQML DPETFLPLD YLQTKPVKE RLRNITEPK GVKLLYDVL QFEPQAVAH AVLFATNNA LVCETPEDA MKVAYELGG RYDAVALDG TYYQKSGII SGGSLDLAR KAKRWDEKH ISQLKAQKE KLTEELRDA MKKSRKESE LNTVDSQIR GLETRLRYA KTDMESTMK QINAVDAEL AKLSDEMEK YGPKIEEIE KTMQTREHQ IEEIKLQMN SVEDVVFSK FCQEIGIRN IRQYEDREL RAQEERKQK RLEFQKQIN RISSNLEFE RSRDTQNNV SRWERTVND EEERLETCK KQEQKQREE IDKDLQQVE QLKAQRLHK KQEVDGMEE ELGKARREV GSIAKDVQA AQKSVVSLE TKIEGKKSE RHAILMQCK MDDVAIPMI VGNMEDIVA SDPSQSSSG DTSSTVQQY EKEARIKID YNMLSDSLK DLEEKDEIK KMADKLLSS IKSLQDTLT KIQAPNMRA IQKLELAQG KLQSTNEEF ENLRKQNKK AKAAFEKMK QQRYERFTR CFDHVSNEI DNIYKALAQ NQSAQAFLG AENPEEPYL DGINYNCVA PGKRFQPMS NLSGGEKTV AALALLFAI HSYQPAPFF VLDEVDAAL DNTNIGKVA KYIRGKTES LQTIVISLK EEFYSHADS LIGICPQPA ECLVSQVLT VDLTKYP
- >TC005672 RA
atgcctccg agattgaaa cacatcgaa gtggagaac ttcaagtcg tacaaaggg caccgaata attgggccc ttgaagcct ttcaatgcc gtaatcggc cccaatggc tcaggtaaa tccaatttt atggacgca attagcttc gttatgggc gaaaagacg caaagtttg cgtgttaag agattgagt gatcttatc catggggct gcgattagc aaaccaata tccagaagt gcttcagtt gctgctgtt tttgttctt gatgaagaa agtggcaaa gaaatttgt ttccagcgt tcggttcaa ggttcttca tctgaatac agaatcaat ggaactgtc gtttcaaac aatgaatat ttaactgaa ctggaaaaa ttacgcatc aatgtaaaa gggaagaat tttctggtc ttccaaggt gcggtagaa tctgttgca atgaagaat cccaaagaa atgaccgca ctttttgag gaaattagc ggttccggt gctttaaaa gaagaatat gatcgcctt aaacagcaa atgcaaaag gcacaagaa gaaatcaac tttgcttat cagaaaaag aaaggcata aatgctgaa cgtaaagaa gcgagactt gagaaagaa gaagctgat aaatattca cgacttaaa gatgatttg aatgacaaa ctggtggag caccaactt ttccgctta taccacaac gagcgcgag atgaaaaac ctggaaaac gatttaaaa cacaaacaa cgcgaggtg gaaaagatc gaaaaaaag aaagaaaaa gccgaagag gttttaaaa gaaaaaaag aaagaacaa ggcaaattt aaccgcgag ttggcaaaa atcgagcaa gatattcgc gaagttgaa gttgagatt tccaaaaaa cggccgcag ttcatcaaa gctaaagaa cgtgtctcc cacatgcag aaaaaatta gacggagcg ataaaaaca ttagaacag gcgagaaaa gcccacgaa gcgcatatg aacgatatt aaaaaactg gaagacgaa ttggccgaa gttgaaaag acgaaagac gaatatgag agtcaaatt gccggagag tcgcaaagt caaggcaga gatgtgcac cttgaagac gaacaggtt agagaatat catcggctg aaagaagag gcagccaag aggtcagcc aggtacatg caggagttg gattctgtc aacagggaa cagaagtcg gatcaggac agacttgac aatgtttca agaatgcgc actgatgcc gaaaataaa catcggcag aagtgtcac gagaaggag gaaatggag aaaagaatt gaaaagctg gccgagcat attcggttg agtgaacag gctttacaa gaccagaaa caactgcgt tctgaccta caatcagac gttgggtca tccaaagac cgcgtccat gaaatccag aaacaactg gacgatgtg ttagaacaa ctgggagat gcaagaacc gacaaacat gaagatgca agacggaag aaaaaacag gagattgtt gagcgcttc aaaagcaac tatcctgga gtttatgat cgcatgatc aacatgtgc caacccatc cataagaga tacaacgtt gccattacg aaagttttg ggtaaattt atggaagct atcgtcgtt gatagcgaa cacacggcc aggcaatgc atcaaatac ctcaaggag caaatgttg gaccctgaa acgttcctc ccacttgac tatttgcag acgaaacct gtcaaggaa cgcttgagg aatataaca gaaccgaaa ggtgtcaaa ctcttgtat gatgttctc cagtttgag cctcaagct gtggcccat gctgtgctt tttgcaact aataatgct ttggtttgt gaaacgcct gaagacgcc atgaaagtg gcgtacgag ttaggtgga cgttacgat gctgtggct cttgatggt acttactac caaaaatct ggcattatt tccggtggt agcttggat ttggcgcga aaagcgaaa cgttgggac gagaaacac atttcacag ttaaaggcc cagaaggag aaacttacc gaagaatta cgtgacgca atgaagaag tcgaggaaa gaatctgaa ctgaacact gtcgattcg caaattcgg ggcctggaa actaggctc agatatgcc aaaactgac atggagagc actatgaaa caaatcaac gctgttgat gccgagctg gccaagcta tcagacgaa atggaaaaa tatgggccc aagattgaa gaaattgaa aaaacgatg caaacacgt gaacaccaa atcgaagaa attaaattg caaatgaac agtgttgaa gatgttgtt ttctcgaaa ttctgccaa gaaattggt attcgtaac attagacag tatgaagat cgcgaattg cgagcgcaa gaagaaagg aagcagaag agattggaa ttccagaag caaatcaat cggatttcg tccaatctc gaatttgaa cgcagtcgg gacacgcag aataatgta agccgttgg gagcgcacc gtaaacgac gaggaagaa cgtttggaa acgtgcaag aaacaggag cagaagcaa cgcgaagaa atcgacaaa gacttgcaa caagtcgag cagttgaaa gcacagcga ctccacaag aagcaggaa gtcgatggt atggaagaa gagctggga aaagcacgc agagaagtg ggaagcatt gcgaaagac gtgcaagcc gcccagaag tccgtagtc tctctcgaa acaaaaatc gaaggcaaa aaaagcgaa cgccacgca attttaatg cagtgcaaa atggacgat gtggctatt cctatgatt gtgggcaac atggaagat atagtggcc tcagacccg tcccaaagc tcgtcaggg gacacaagt agcacagtt caacaatac gagaaagaa gcgaggatt aaaatcgac tacaacatg ttgtcagac agtctcaaa gacctggag gaaaaagac gagattaag aaaatggca gataaactc ctcagctcg attaagagt ctccaagac actttgacc aaaattcaa gcgcctaac atgcgagcg attcagaag ctcgagctc gcgcaaggg aaactccaa tcgacgaac gaggagttt gagaacttg cgtaagcag aataagaaa gcgaaagca gcgtttgag aaaatgaaa cagcaacgg tatgaacgt ttcacgcgg tgttttgat cacgtgtcg aacgaaatc gataacatt tacaaagct ttggcacag aatcagtca gctcaggcg tttctgggc gctgaaaac ccagaagag ccctatttg gatgggatt aattacaat tgtgtggct ccagggaag aggtttcag ccaatgtcg aacctgtca ggtggtgag aaaacagtg gcagctttg gcgttgctt tttgcgata catagttat caaccggca ccgtttttt gtgcttgat gaggtcgat gctgccttg gataatacc aatatagga aaagtggcc aagtatata aggggcaag actgagtct ttgcagact atagttatt tcgttgaag gaggaattt tattcgcat gctgattct ttgattggg atttgtcct cagccggct gaatgttta gtcagtcag gttctaaca gttgattta acgaaatat ccataa
- >TC005672 RA
ctagccgca gccgccata cttgttgtt tgtggttgt gcggtgttt ctgtttaaa aaataatgc aaaatctgt aaaaaccca caaaatgcc tccgagatt gaaacacat cgaagtgga gaacttcaa gtcgtacaa agggcaccg aataattgg gcccttgaa gcctttcaa tgccgtaat cggccccaa tggctcagg taaatccaa ttttatgga cgcaattag cttcgttat gggcgaaaa gacgcaaag tttgcgtgt taagagatt gagtgatct tatccatgg ggctgcgat tagcaaacc aatatccag aagtgcttc agttgctgc tgtttttgt tcttgatga agaaagtgg caaagaaat ttgtttcca gcgttcggt tcaaggttc ttcatctga atacagaat caatggaac tgtcgtttc aaacaatga atatttaac tgaactgga aaaattacg catcaatgt aaaagggaa gaattttct ggtcttcca aggtgcggt agaatctgt tgcaatgaa gaatcccaa agaaatgac cgcactttt tgaggaaat tagcggttc cggtgcttt aaaagaaga atatgatcg ccttaaaca gcaaatgca aaaggcaca agaagaaat caactttgc ttatcagaa aaagaaagg cataaatgc tgaacgtaa agaagcgag acttgagaa agaagaagc tgataaata ttcacgact taaagatga tttgaatga caaactggt ggagcacca acttttccg cttatacca caacgagcg cgagatgaa aaacctgga aaacgattt aaaacacaa acaacgcga ggtggaaaa gatcgaaaa aaagaaaga aaaagccga agaggtttt aaaagaaaa aaagaaaga acaaggcaa atttaaccg cgagttggc aaaaatcga gcaagatat tcgcgaagt tgaagttga gatttccaa aaaacggcc gcagttcat caaagctaa agaacgtgt ctcccacat gcagaaaaa attagacgg agcgataaa aacattaga acaggcgag aaaagccca cgaagcgca tatgaacga tattaaaaa actggaaga cgaattggc cgaagttga aaagacgaa agacgaata tgagagtca aattgccgg agagtcgca aagtcaagg cagagatgt gcaccttga agacgaaca ggttagaga atatcatcg gctgaaaga agaggcagc caagaggtc agccaggta catgcagga gttggattc tgtcaacag ggaacagaa gtcggatca ggacagact tgacaatgt ttcaagaat gcgcactga tgccgaaaa taaacatcg gcagaagtg tcacgagaa ggaggaaat ggagaaaag aattgaaaa gctggccga gcatattcg gttgagtga acaggcttt acaagacca gaaacaact gcgttctga cctacaatc agacgttgg gtcatccaa agaccgcgt ccatgaaat ccagaaaca actggacga tgtgttaga acaactggg agatgcaag aaccgacaa acatgaaga tgcaagacg gaagaaaaa acaggagat tgttgagcg cttcaaaag caactatcc tggagttta tgatcgcat gatcaacat gtgccaacc catccataa gagatacaa cgttgccat tacgaaagt tttgggtaa atttatgga agctatcgt cgttgatag cgaacacac ggccaggca atgcatcaa atacctcaa ggagcaaat gttggaccc tgaaacgtt cctcccact tgactattt gcagacgaa acctgtcaa ggaacgctt gaggaatat aacagaacc gaaaggtgt caaactctt gtatgatgt tctccagtt tgagcctca agctgtggc ccatgctgt gctttttgc aactaataa tgctttggt ttgtgaaac gcctgaaga cgccatgaa agtggcgta cgagttagg tggacgtta cgatgctgt ggctcttga tggtactta ctaccaaaa atctggcat tatttccgg tggtagctt ggatttggc gcgaaaagc gaaacgttg ggacgagaa acacatttc acagttaaa ggcccagaa ggagaaact taccgaaga attacgtga cgcaatgaa gaagtcgag gaaagaatc tgaactgaa cactgtcga ttcgcaaat tcggggcct ggaaactag gctcagata tgccaaaac tgacatgga gagcactat gaaacaaat caacgctgt tgatgccga gctggccaa gctatcaga cgaaatgga aaaatatgg gcccaagat tgaagaaat tgaaaaaac gatgcaaac acgtgaaca ccaaatcga agaaattaa attgcaaat gaacagtgt tgaagatgt tgttttctc gaaattctg ccaagaaat tggtattcg taacattag acagtatga agatcgcga attgcgagc gcaagaaga aaggaagca gaagagatt ggaattcca gaagcaaat caatcggat ttcgtccaa tctcgaatt tgaacgcag tcgggacac gcagaataa tgtaagccg ttgggagcg caccgtaaa cgacgagga agaacgttt ggaaacgtg caagaaaca ggagcagaa gcaacgcga agaaatcga caaagactt gcaacaagt cgagcagtt gaaagcaca gcgactcca caagaagca ggaagtcga tggtatgga agaagagct gggaaaagc acgcagaga agtgggaag cattgcgaa agacgtgca agccgccca gaagtccgt agtctctct cgaaacaaa aatcgaagg caaaaaaag cgaacgcca cgcaatttt aatgcagtg caaaatgga cgatgtggc tattcctat gattgtggg caacatgga agatatagt ggcctcaga cccgtccca aagctcgtc aggggacac aagtagcac agttcaaca atacgagaa agaagcgag gattaaaat cgactacaa catgttgtc agacagtct caaagacct ggaggaaaa agacgagat taagaaaat ggcagataa actcctcag ctcgattaa gagtctcca agacacttt gaccaaaat tcaagcgcc taacatgcg agcgattca gaagctcga gctcgcgca agggaaact ccaatcgac gaacgagga gtttgagaa cttgcgtaa gcagaataa gaaagcgaa agcagcgtt tgagaaaat gaaacagca acggtatga acgtttcac gcggtgttt tgatcacgt gtcgaacga aatcgataa catttacaa agctttggc acagaatca gtcagctca ggcgtttct gggcgctga aaacccaga agagcccta tttggatgg gattaatta caattgtgt ggctccagg gaagaggtt tcagccaat gtcgaacct gtcaggtgg tgagaaaac agtggcagc tttggcgtt gctttttgc gatacatag ttatcaacc ggcaccgtt ttttgtgct tgatgaggt cgatgctgc cttggataa taccaatat aggaaaagt ggccaagta tataagggg caagactga gtctttgca gactatagt tatttcgtt gaaggagga attttattc gcatgctga ttctttgat tgggatttg tcctcagcc ggctgaatg tttagtcag tcaggttct aacagttga tttaacgaa atatccata agtattgtt taatttatg tttttacgt ttcgagctt tcaataaaa ctttgtgta ctattttgg ggagcttat caagtctag gaaatgttt gcttctaca tttctagta ttagcactg aagtcttga accgataaa atagcagaa aagtacata aagttttga caatcaaaa tttttcgga tttttcgaa tttttcgga gctaaaaca aatacaatt cgtggcaat aaagatcgc tacatttta atcttgttt gtttaaata aaaaacata cttttcaag tttccatta actgcgaaa gcttgataa cgtagacca cggccacct aaattccaa ttcgaacaa atgagactt ggcagcaaa tccaattcg agcgaattg tgaatttgt aattattga taatagcgc aaaattgtg ttatttttc attgttttt agcctataa gtatgataa ataaagccc taagaacat accctacaa cttttgagg aaaactatt gcaaaaaat gttgaggtt agaaaagct cgaagctga acctgtgcc ccacttttc atatggaat tgctaatta tatcgttta taacataat agagctcaa atcagaggg ctatctgtg tctacggaa tagtctaga atttgccac attccgatc ccaatttcc ctgcacgat ttttttttc tatttattt gtttaattt aaaattgta atttaatat aacatcgat ttgacgatt tgtacaaaa agaaaattt gtttgaata aaaatatta cacaata
