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Gene information
General information
TC005672 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9822341..9831840
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005672
    iBeetle screen iB_00906

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005672 RA
      MPPRLKHIE VENFKSYKG HRIIGPLKP FNAVIGPNG 
      SGKSNFMDA ISFVMGEKT QSLRVKRLS DLIHGAAIS 
      KPISRSASV AAVFVLDEE SGKEICFQR SVQGSSSEY 
      RINGTVVSN NEYLTELEK LRINVKGKN FLVFQGAVE 
      SVAMKNPKE MTALFEEIS GSGALKEEY DRLKQQMQK 
      AQEEINFAY QKKKGINAE RKEARLEKE EADKYSRLK 
      DDLNDKLVE HQLFRLYHN EREMKNLEN DLKHKQREV 
      EKIEKKKEK AEEVLKEKK KEQGKFNRE LAKIEQDIR 
      EVEVEISKK RPQFIKAKE RVSHMQKKL DGAIKTLEQ 
      ARKAHEAHM NDIKKLEDE LAEVEKTKD EYESQIAGE 
      SQSQGRDVH LEDEQVREY HRLKEEAAK RSARYMQEL 
      DSVNREQKS DQDRLDNVS RMRTDAENK HRQKCHEKE 
      EMEKRIEKL AEHIRLSEQ ALQDQKQLR SDLQSDVGS 
      SKDRVHEIQ KQLDDVLEQ LGDARTDKH EDARRKKKQ 
      EIVERFKSN YPGVYDRMI NMCQPIHKR YNVAITKVL 
      GKFMEAIVV DSEHTARQC IKYLKEQML DPETFLPLD 
      YLQTKPVKE RLRNITEPK GVKLLYDVL QFEPQAVAH 
      AVLFATNNA LVCETPEDA MKVAYELGG RYDAVALDG 
      TYYQKSGII SGGSLDLAR KAKRWDEKH ISQLKAQKE 
      KLTEELRDA MKKSRKESE LNTVDSQIR GLETRLRYA 
      KTDMESTMK QINAVDAEL AKLSDEMEK YGPKIEEIE 
      KTMQTREHQ IEEIKLQMN SVEDVVFSK FCQEIGIRN 
      IRQYEDREL RAQEERKQK RLEFQKQIN RISSNLEFE 
      RSRDTQNNV SRWERTVND EEERLETCK KQEQKQREE 
      IDKDLQQVE QLKAQRLHK KQEVDGMEE ELGKARREV 
      GSIAKDVQA AQKSVVSLE TKIEGKKSE RHAILMQCK 
      MDDVAIPMI VGNMEDIVA SDPSQSSSG DTSSTVQQY 
      EKEARIKID YNMLSDSLK DLEEKDEIK KMADKLLSS 
      IKSLQDTLT KIQAPNMRA IQKLELAQG KLQSTNEEF 
      ENLRKQNKK AKAAFEKMK QQRYERFTR CFDHVSNEI 
      DNIYKALAQ NQSAQAFLG AENPEEPYL DGINYNCVA 
      PGKRFQPMS NLSGGEKTV AALALLFAI HSYQPAPFF 
      VLDEVDAAL DNTNIGKVA KYIRGKTES LQTIVISLK 
      EEFYSHADS LIGICPQPA ECLVSQVLT VDLTKYP
    CDS sequence
    • >TC005672 RA
      atgcctccg agattgaaa cacatcgaa gtggagaac 
      ttcaagtcg tacaaaggg caccgaata attgggccc 
      ttgaagcct ttcaatgcc gtaatcggc cccaatggc 
      tcaggtaaa tccaatttt atggacgca attagcttc 
      gttatgggc gaaaagacg caaagtttg cgtgttaag 
      agattgagt gatcttatc catggggct gcgattagc 
      aaaccaata tccagaagt gcttcagtt gctgctgtt 
      tttgttctt gatgaagaa agtggcaaa gaaatttgt 
      ttccagcgt tcggttcaa ggttcttca tctgaatac 
      agaatcaat ggaactgtc gtttcaaac aatgaatat 
      ttaactgaa ctggaaaaa ttacgcatc aatgtaaaa 
      gggaagaat tttctggtc ttccaaggt gcggtagaa 
      tctgttgca atgaagaat cccaaagaa atgaccgca 
      ctttttgag gaaattagc ggttccggt gctttaaaa 
      gaagaatat gatcgcctt aaacagcaa atgcaaaag 
      gcacaagaa gaaatcaac tttgcttat cagaaaaag 
      aaaggcata aatgctgaa cgtaaagaa gcgagactt 
      gagaaagaa gaagctgat aaatattca cgacttaaa 
      gatgatttg aatgacaaa ctggtggag caccaactt 
      ttccgctta taccacaac gagcgcgag atgaaaaac 
      ctggaaaac gatttaaaa cacaaacaa cgcgaggtg 
      gaaaagatc gaaaaaaag aaagaaaaa gccgaagag 
      gttttaaaa gaaaaaaag aaagaacaa ggcaaattt 
      aaccgcgag ttggcaaaa atcgagcaa gatattcgc 
      gaagttgaa gttgagatt tccaaaaaa cggccgcag 
      ttcatcaaa gctaaagaa cgtgtctcc cacatgcag 
      aaaaaatta gacggagcg ataaaaaca ttagaacag 
      gcgagaaaa gcccacgaa gcgcatatg aacgatatt 
      aaaaaactg gaagacgaa ttggccgaa gttgaaaag 
      acgaaagac gaatatgag agtcaaatt gccggagag 
      tcgcaaagt caaggcaga gatgtgcac cttgaagac 
      gaacaggtt agagaatat catcggctg aaagaagag 
      gcagccaag aggtcagcc aggtacatg caggagttg 
      gattctgtc aacagggaa cagaagtcg gatcaggac 
      agacttgac aatgtttca agaatgcgc actgatgcc 
      gaaaataaa catcggcag aagtgtcac gagaaggag 
      gaaatggag aaaagaatt gaaaagctg gccgagcat 
      attcggttg agtgaacag gctttacaa gaccagaaa 
      caactgcgt tctgaccta caatcagac gttgggtca 
      tccaaagac cgcgtccat gaaatccag aaacaactg 
      gacgatgtg ttagaacaa ctgggagat gcaagaacc 
      gacaaacat gaagatgca agacggaag aaaaaacag 
      gagattgtt gagcgcttc aaaagcaac tatcctgga 
      gtttatgat cgcatgatc aacatgtgc caacccatc 
      cataagaga tacaacgtt gccattacg aaagttttg 
      ggtaaattt atggaagct atcgtcgtt gatagcgaa 
      cacacggcc aggcaatgc atcaaatac ctcaaggag 
      caaatgttg gaccctgaa acgttcctc ccacttgac 
      tatttgcag acgaaacct gtcaaggaa cgcttgagg 
      aatataaca gaaccgaaa ggtgtcaaa ctcttgtat 
      gatgttctc cagtttgag cctcaagct gtggcccat 
      gctgtgctt tttgcaact aataatgct ttggtttgt 
      gaaacgcct gaagacgcc atgaaagtg gcgtacgag 
      ttaggtgga cgttacgat gctgtggct cttgatggt 
      acttactac caaaaatct ggcattatt tccggtggt 
      agcttggat ttggcgcga aaagcgaaa cgttgggac 
      gagaaacac atttcacag ttaaaggcc cagaaggag 
      aaacttacc gaagaatta cgtgacgca atgaagaag 
      tcgaggaaa gaatctgaa ctgaacact gtcgattcg 
      caaattcgg ggcctggaa actaggctc agatatgcc 
      aaaactgac atggagagc actatgaaa caaatcaac 
      gctgttgat gccgagctg gccaagcta tcagacgaa 
      atggaaaaa tatgggccc aagattgaa gaaattgaa 
      aaaacgatg caaacacgt gaacaccaa atcgaagaa 
      attaaattg caaatgaac agtgttgaa gatgttgtt 
      ttctcgaaa ttctgccaa gaaattggt attcgtaac 
      attagacag tatgaagat cgcgaattg cgagcgcaa 
      gaagaaagg aagcagaag agattggaa ttccagaag 
      caaatcaat cggatttcg tccaatctc gaatttgaa 
      cgcagtcgg gacacgcag aataatgta agccgttgg 
      gagcgcacc gtaaacgac gaggaagaa cgtttggaa 
      acgtgcaag aaacaggag cagaagcaa cgcgaagaa 
      atcgacaaa gacttgcaa caagtcgag cagttgaaa 
      gcacagcga ctccacaag aagcaggaa gtcgatggt 
      atggaagaa gagctggga aaagcacgc agagaagtg 
      ggaagcatt gcgaaagac gtgcaagcc gcccagaag 
      tccgtagtc tctctcgaa acaaaaatc gaaggcaaa 
      aaaagcgaa cgccacgca attttaatg cagtgcaaa 
      atggacgat gtggctatt cctatgatt gtgggcaac 
      atggaagat atagtggcc tcagacccg tcccaaagc 
      tcgtcaggg gacacaagt agcacagtt caacaatac 
      gagaaagaa gcgaggatt aaaatcgac tacaacatg 
      ttgtcagac agtctcaaa gacctggag gaaaaagac 
      gagattaag aaaatggca gataaactc ctcagctcg 
      attaagagt ctccaagac actttgacc aaaattcaa 
      gcgcctaac atgcgagcg attcagaag ctcgagctc 
      gcgcaaggg aaactccaa tcgacgaac gaggagttt 
      gagaacttg cgtaagcag aataagaaa gcgaaagca 
      gcgtttgag aaaatgaaa cagcaacgg tatgaacgt 
      ttcacgcgg tgttttgat cacgtgtcg aacgaaatc 
      gataacatt tacaaagct ttggcacag aatcagtca 
      gctcaggcg tttctgggc gctgaaaac ccagaagag 
      ccctatttg gatgggatt aattacaat tgtgtggct 
      ccagggaag aggtttcag ccaatgtcg aacctgtca 
      ggtggtgag aaaacagtg gcagctttg gcgttgctt 
      tttgcgata catagttat caaccggca ccgtttttt 
      gtgcttgat gaggtcgat gctgccttg gataatacc 
      aatatagga aaagtggcc aagtatata aggggcaag 
      actgagtct ttgcagact atagttatt tcgttgaag 
      gaggaattt tattcgcat gctgattct ttgattggg 
      atttgtcct cagccggct gaatgttta gtcagtcag 
      gttctaaca gttgattta acgaaatat ccataa
    mRNA sequence
    • >TC005672 RA
      ctagccgca gccgccata cttgttgtt tgtggttgt 
      gcggtgttt ctgtttaaa aaataatgc aaaatctgt 
      aaaaaccca caaaatgcc tccgagatt gaaacacat 
      cgaagtgga gaacttcaa gtcgtacaa agggcaccg 
      aataattgg gcccttgaa gcctttcaa tgccgtaat 
      cggccccaa tggctcagg taaatccaa ttttatgga 
      cgcaattag cttcgttat gggcgaaaa gacgcaaag 
      tttgcgtgt taagagatt gagtgatct tatccatgg 
      ggctgcgat tagcaaacc aatatccag aagtgcttc 
      agttgctgc tgtttttgt tcttgatga agaaagtgg 
      caaagaaat ttgtttcca gcgttcggt tcaaggttc 
      ttcatctga atacagaat caatggaac tgtcgtttc 
      aaacaatga atatttaac tgaactgga aaaattacg 
      catcaatgt aaaagggaa gaattttct ggtcttcca 
      aggtgcggt agaatctgt tgcaatgaa gaatcccaa 
      agaaatgac cgcactttt tgaggaaat tagcggttc 
      cggtgcttt aaaagaaga atatgatcg ccttaaaca 
      gcaaatgca aaaggcaca agaagaaat caactttgc 
      ttatcagaa aaagaaagg cataaatgc tgaacgtaa 
      agaagcgag acttgagaa agaagaagc tgataaata 
      ttcacgact taaagatga tttgaatga caaactggt 
      ggagcacca acttttccg cttatacca caacgagcg 
      cgagatgaa aaacctgga aaacgattt aaaacacaa 
      acaacgcga ggtggaaaa gatcgaaaa aaagaaaga 
      aaaagccga agaggtttt aaaagaaaa aaagaaaga 
      acaaggcaa atttaaccg cgagttggc aaaaatcga 
      gcaagatat tcgcgaagt tgaagttga gatttccaa 
      aaaacggcc gcagttcat caaagctaa agaacgtgt 
      ctcccacat gcagaaaaa attagacgg agcgataaa 
      aacattaga acaggcgag aaaagccca cgaagcgca 
      tatgaacga tattaaaaa actggaaga cgaattggc 
      cgaagttga aaagacgaa agacgaata tgagagtca 
      aattgccgg agagtcgca aagtcaagg cagagatgt 
      gcaccttga agacgaaca ggttagaga atatcatcg 
      gctgaaaga agaggcagc caagaggtc agccaggta 
      catgcagga gttggattc tgtcaacag ggaacagaa 
      gtcggatca ggacagact tgacaatgt ttcaagaat 
      gcgcactga tgccgaaaa taaacatcg gcagaagtg 
      tcacgagaa ggaggaaat ggagaaaag aattgaaaa 
      gctggccga gcatattcg gttgagtga acaggcttt 
      acaagacca gaaacaact gcgttctga cctacaatc 
      agacgttgg gtcatccaa agaccgcgt ccatgaaat 
      ccagaaaca actggacga tgtgttaga acaactggg 
      agatgcaag aaccgacaa acatgaaga tgcaagacg 
      gaagaaaaa acaggagat tgttgagcg cttcaaaag 
      caactatcc tggagttta tgatcgcat gatcaacat 
      gtgccaacc catccataa gagatacaa cgttgccat 
      tacgaaagt tttgggtaa atttatgga agctatcgt 
      cgttgatag cgaacacac ggccaggca atgcatcaa 
      atacctcaa ggagcaaat gttggaccc tgaaacgtt 
      cctcccact tgactattt gcagacgaa acctgtcaa 
      ggaacgctt gaggaatat aacagaacc gaaaggtgt 
      caaactctt gtatgatgt tctccagtt tgagcctca 
      agctgtggc ccatgctgt gctttttgc aactaataa 
      tgctttggt ttgtgaaac gcctgaaga cgccatgaa 
      agtggcgta cgagttagg tggacgtta cgatgctgt 
      ggctcttga tggtactta ctaccaaaa atctggcat 
      tatttccgg tggtagctt ggatttggc gcgaaaagc 
      gaaacgttg ggacgagaa acacatttc acagttaaa 
      ggcccagaa ggagaaact taccgaaga attacgtga 
      cgcaatgaa gaagtcgag gaaagaatc tgaactgaa 
      cactgtcga ttcgcaaat tcggggcct ggaaactag 
      gctcagata tgccaaaac tgacatgga gagcactat 
      gaaacaaat caacgctgt tgatgccga gctggccaa 
      gctatcaga cgaaatgga aaaatatgg gcccaagat 
      tgaagaaat tgaaaaaac gatgcaaac acgtgaaca 
      ccaaatcga agaaattaa attgcaaat gaacagtgt 
      tgaagatgt tgttttctc gaaattctg ccaagaaat 
      tggtattcg taacattag acagtatga agatcgcga 
      attgcgagc gcaagaaga aaggaagca gaagagatt 
      ggaattcca gaagcaaat caatcggat ttcgtccaa 
      tctcgaatt tgaacgcag tcgggacac gcagaataa 
      tgtaagccg ttgggagcg caccgtaaa cgacgagga 
      agaacgttt ggaaacgtg caagaaaca ggagcagaa 
      gcaacgcga agaaatcga caaagactt gcaacaagt 
      cgagcagtt gaaagcaca gcgactcca caagaagca 
      ggaagtcga tggtatgga agaagagct gggaaaagc 
      acgcagaga agtgggaag cattgcgaa agacgtgca 
      agccgccca gaagtccgt agtctctct cgaaacaaa 
      aatcgaagg caaaaaaag cgaacgcca cgcaatttt 
      aatgcagtg caaaatgga cgatgtggc tattcctat 
      gattgtggg caacatgga agatatagt ggcctcaga 
      cccgtccca aagctcgtc aggggacac aagtagcac 
      agttcaaca atacgagaa agaagcgag gattaaaat 
      cgactacaa catgttgtc agacagtct caaagacct 
      ggaggaaaa agacgagat taagaaaat ggcagataa 
      actcctcag ctcgattaa gagtctcca agacacttt 
      gaccaaaat tcaagcgcc taacatgcg agcgattca 
      gaagctcga gctcgcgca agggaaact ccaatcgac 
      gaacgagga gtttgagaa cttgcgtaa gcagaataa 
      gaaagcgaa agcagcgtt tgagaaaat gaaacagca 
      acggtatga acgtttcac gcggtgttt tgatcacgt 
      gtcgaacga aatcgataa catttacaa agctttggc 
      acagaatca gtcagctca ggcgtttct gggcgctga 
      aaacccaga agagcccta tttggatgg gattaatta 
      caattgtgt ggctccagg gaagaggtt tcagccaat 
      gtcgaacct gtcaggtgg tgagaaaac agtggcagc 
      tttggcgtt gctttttgc gatacatag ttatcaacc 
      ggcaccgtt ttttgtgct tgatgaggt cgatgctgc 
      cttggataa taccaatat aggaaaagt ggccaagta 
      tataagggg caagactga gtctttgca gactatagt 
      tatttcgtt gaaggagga attttattc gcatgctga 
      ttctttgat tgggatttg tcctcagcc ggctgaatg 
      tttagtcag tcaggttct aacagttga tttaacgaa 
      atatccata agtattgtt taatttatg tttttacgt 
      ttcgagctt tcaataaaa ctttgtgta ctattttgg 
      ggagcttat caagtctag gaaatgttt gcttctaca 
      tttctagta ttagcactg aagtcttga accgataaa 
      atagcagaa aagtacata aagttttga caatcaaaa 
      tttttcgga tttttcgaa tttttcgga gctaaaaca 
      aatacaatt cgtggcaat aaagatcgc tacatttta 
      atcttgttt gtttaaata aaaaacata cttttcaag 
      tttccatta actgcgaaa gcttgataa cgtagacca 
      cggccacct aaattccaa ttcgaacaa atgagactt 
      ggcagcaaa tccaattcg agcgaattg tgaatttgt 
      aattattga taatagcgc aaaattgtg ttatttttc 
      attgttttt agcctataa gtatgataa ataaagccc 
      taagaacat accctacaa cttttgagg aaaactatt 
      gcaaaaaat gttgaggtt agaaaagct cgaagctga 
      acctgtgcc ccacttttc atatggaat tgctaatta 
      tatcgttta taacataat agagctcaa atcagaggg 
      ctatctgtg tctacggaa tagtctaga atttgccac 
      attccgatc ccaatttcc ctgcacgat ttttttttc 
      tatttattt gtttaattt aaaattgta atttaatat 
      aacatcgat ttgacgatt tgtacaaaa agaaaattt 
      gtttgaata aaaatatta cacaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY