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Gene information
General information
TC005644 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10260599..10263718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005644
    iBeetle screen iB_00902

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax anterior partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005644 RA
      MQRENLREN MLLGMGNPL LDISATVDK DFLTKYNMK 
      ENNAILADE SHKNLNSEM IEKYKAEFI AGGSVQNSL 
      RVAQWLLQK PKVTTFFGC VGTDKYSQI LKDKAKADG 
      VNVVYQYND KVPTGTCAV LITGTNRSL CANLAAANC 
      FTIDHIRDP ENRKLLESA QYFYISGFF ITVSPQSIL 
      EVAKHALAN DRPFIMNLS APFISQFYK EPLMQAMPY 
      VDLLFGNET EAETFANEQ NFGTKDLKE IALKICNLP 
      KQNENRSRV CVITTGHNP VILAREGKI SEFPVDVLS 
      KDKLVDTNG AGDAFAGGF LSQYIQGQS LDVCVRCGI 
      WAASQIVQR SGCTFSGKA NFQP
    CDS sequence
    • >TC005644 RA
      atgcaacgt gaaaattta cgggagaac atgttgctg 
      ggcatggga aacccctta ctggacatc tccgccacg 
      gtggacaag gacttccta accaaatac aacatgaaa 
      gagaacaac gcgatcttg gccgacgag tcgcacaaa 
      aatctcaac tcggaaatg atcgaaaag tacaaagcg 
      gagtttata gccggcggc agcgtccag aacagtctc 
      cgagtggcg cagtggctg ctccagaag cccaaagtg 
      accacgttt ttcgggtgc gtcgggact gataaatac 
      tcccagatt ttgaaagac aaggcgaaa gccgacggt 
      gttaacgtg gtgtatcag tacaacgac aaggtcccc 
      acagggacc tgtgccgtg cttatcacg ggcacgaac 
      cgctccttg tgtgccaac ttagcagcg gcgaactgc 
      ttcacaatt gatcatatc agagacccg gaaaatcgc 
      aagttgtta gagagtgca caatatttt tacatctcg 
      gggttcttc atcaccgtg agtccgcaa agcatactc 
      gaagttgcg aaacatgcc ttggccaac gatcgcccc 
      tttattatg aatctcagc gcgccgttc atttcacaa 
      ttctacaag gagcctctg atgcaagcg atgccttac 
      gttgacctc ttatttggg aacgaaacg gaggcggag 
      acgttcgcc aacgagcag aacttcggc acgaaagac 
      ttaaaagag atcgctttg aaaatctgc aatcttcca 
      aagcagaac gagaaccga tcgcgcgtc tgcgtcatc 
      accacgggg cacaacccg gtgatcctc gcccgtgag 
      gggaaaatc agcgagttt cccgtcgat gttctgagc 
      aaggacaag ctggttgac accaacgga gctggcgac 
      gcgttcgct ggcggcttc ctctcgcag tacatccaa 
      gggcagagt ctggacgtt tgcgtgcga tgcggcatc 
      tgggcggcg tcgcagatc gtgcagagg agcgggtgc 
      accttcagc gggaaggcc aatttccaa ccttag
    mRNA sequence
    • >TC005644 RA
      cgagacgta tgtcgcgaa ttgcgcgca gcgctgatt 
      aaggtgttt gactgcagt ggccccaat attgaagca 
      gtcggagtg tcgccatct ctccgcgga gcctcacac 
      ttcgaacat tacttaaca atttcataa tacctcatt 
      aaatattca ttatctcga aaaccagta aacaataag 
      gctaaaaat accaacaat ggttctgtg aacgatatt 
      taacaagcg gggtgaagg tggcatgtt actcgccga 
      gacgttctc ctgacagtc cgccagtga ggttatgtc 
      cgtcaaaat tccaccaaa ctgcttcaa acccaaagt 
      tcgcgctat ctagcccac tctcaaaca tgcaacgtg 
      aaaatttac gggagaaca tgttgctgg gcatgggaa 
      accccttac tggacatct ccgccacgg tggacaagg 
      acttcctaa ccaaataca acatgaaag agaacaacg 
      cgatcttgg ccgacgagt cgcacaaaa atctcaact 
      cggaaatga tcgaaaagt acaaagcgg agtttatag 
      ccggcggca gcgtccaga acagtctcc gagtggcgc 
      agtggctgc tccagaagc ccaaagtga ccacgtttt 
      tcgggtgcg tcgggactg ataaatact cccagattt 
      tgaaagaca aggcgaaag ccgacggtg ttaacgtgg 
      tgtatcagt acaacgaca aggtcccca cagggacct 
      gtgccgtgc ttatcacgg gcacgaacc gctccttgt 
      gtgccaact tagcagcgg cgaactgct tcacaattg 
      atcatatca gagacccgg aaaatcgca agttgttag 
      agagtgcac aatattttt acatctcgg ggttcttca 
      tcaccgtga gtccgcaaa gcatactcg aagttgcga 
      aacatgcct tggccaacg atcgcccct ttattatga 
      atctcagcg cgccgttca tttcacaat tctacaagg 
      agcctctga tgcaagcga tgccttacg ttgacctct 
      tatttggga acgaaacgg aggcggaga cgttcgcca 
      acgagcaga acttcggca cgaaagact taaaagaga 
      tcgctttga aaatctgca atcttccaa agcagaacg 
      agaaccgat cgcgcgtct gcgtcatca ccacggggc 
      acaacccgg tgatcctcg cccgtgagg ggaaaatca 
      gcgagtttc ccgtcgatg ttctgagca aggacaagc 
      tggttgaca ccaacggag ctggcgacg cgttcgctg 
      gcggcttcc tctcgcagt acatccaag ggcagagtc 
      tggacgttt gcgtgcgat gcggcatct gggcggcgt 
      cgcagatcg tgcagagga gcgggtgca ccttcagcg 
      ggaaggcca atttccaac cttagcaat agtattcag 
      ggcttagta cttagtttt tttatcgca ctgttgagt 
      aagcaccaa taaactgtt gaatgaaag tcggcgtaa 
      aaactaaca ttttttaat atcatgtag ttatacaaa 
      tttgaatat acagcgaaa ctgctcata cacaatgtt 
      aaagaaaca agctaacaa atctaatca cagtgctac 
      acgaaataa aaataagtg ataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY