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Gene information
General information
TC005593 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3830023..3831660
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005593
    iBeetle screen iB_00894
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    egg chamber mostly not present - Number of animals showing the phenotype: 4
    vitellarium thinner - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 2

    wings shortened - Number of animals showing the phenotype: 2

    elytra partiallyblistered - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005593 RA
      MANFDEIER KLRQLDQSH LLQFFYKFS DEEKKNFLQ 
      HLQGLDLDA SARLFERAK NCLDVAPDT EDMKPIPHS 
      QFESEEGCD GETLERYRI RGLEAIGAG EVGVLLLAG 
      GQGTRLGVT YPKGMYSVG LPSGKTIFQ IQAERIRRV 
      QHLAKKHTG KGGKVTWYI MTSGPTDKM TETFLKSHN 
      FFGLDPQNV VLFKQGLLP CFDFDGKII LEAPNLVAL 
      APDGNGGIY RALHVNGVL DDMRRRGVK YIHAHSVDN 
      ILTKVADPV FIGYFIEKG GDCAAKVVK KAGPTEAVG 
      VVCQIKGRF QVVEYSEIS EEKAHLRDE EGNLVYSAG 
      NICNHLFTT VFLQRVSDE FEHELKLHV AKKKIPFVD 
      ETGQTVTPE KPNGIKIEK FIFDVFPFS DRFVTWEVP 
      RKSEFSAMK NMDSVGKDC PSTARQDLL ALHRTYIEK 
      AGGVVSAEV EISPLLSYT GEELEARVK GKMFTETTV 
      LLGEEEISI NGGRA
    CDS sequence
    • >TC005593 RA
      atggcaaat ttcgatgaa attgaaaga aaactgaga 
      caactggac cagagccac ttattgcag tttttttac 
      aagtttagt gatgaggaa aaaaagaat ttcttgcaa 
      cacttgcaa ggtttggat ttggacgcc agtgccagg 
      ttgtttgaa cgagcgaag aattgtctt gacgtggct 
      ccggacacc gaagacatg aagccgatt ccgcattcg 
      cagttcgag tcggaggag ggttgtgac ggtgaaacg 
      ctcgaaagg tacagaata cgggggttg gaggcgatc 
      ggcgccggt gaagtgggt gtcctgctt ctggcaggg 
      gggcaaggc acccggctg ggggtcacg taccccaag 
      ggcatgtac tcggtcggc ttgccctcc gggaaaacc 
      atcttccaa atccaggcc gaacgcatc agacgggtc 
      caacacttg gctaagaaa cacacaggg aagggtggc 
      aaagtcact tggtatatc atgacaagt ggccccact 
      gataaaatg actgaaaca ttcctcaaa tcgcacaat 
      ttcttcggc cttgacccc caaaacgtc gttttgttc 
      aagcagggc ctactgccc tgtttcgat ttcgacggg 
      aagataatc ctagaggcc ccgaatcta gttgcctta 
      gccccggac ggaaacggc gggatatac cgtgcgttg 
      cacgtgaac ggtgtttta gacgacatg cggaggcgt 
      ggcgtgaag tacattcac gcccacagc gtcgataac 
      atcctcacc aaagtcgcc gacccggtt ttcattggc 
      tactttatt gaaaagggt ggggattgc gcggccaaa 
      gtggtcaaa aaggcggga ccaacggaa gcggtgggc 
      gtggtgtgc caaatcaaa gggcggttc caagtcgtt 
      gaatacagc gaaatttcg gaagaaaag gcgcatttg 
      cgtgacgag gaggggaat ctggtttat agtgctggc 
      aacatttgc aaccattta ttcacaact gtgtttcta 
      caaagggtc agcgatgaa ttcgaacac gaattgaag 
      ttacacgtg gctaagaaa aaaattcct ttcgttgac 
      gaaaccgga caaacagtc acaccggaa aaacccaac 
      ggaattaaa atagaaaaa ttcattttc gacgttttc 
      ccattcagt gatcggttc gtgacgtgg gaagtgccc 
      cgtaaaagt gaattcagt gcaatgaaa aatatggat 
      agtgtcggt aaagactgc ccttccacg gcacgccag 
      gatttacta gccttgcat cggacttat attgaaaaa 
      gccgggggc gtggtttcc gcagaggtg gagatttcg 
      cccctcctg tcgtacaca ggggaggag cttgaagcc 
      cgcgtcaaa ggaaaaatg tttacagaa acgaccgtt 
      ttattggga gaggaagag attagtatt aatggtggg 
      agggcttaa
    mRNA sequence
    • >TC005593 RA
      tgacgtttg tttgacagt aatttgaca gctatcttt 
      tatttgaaa taatcccag tgacacgat ggcaaattt 
      cgatgaaat tgaaagaaa actgagaca actggacca 
      gagccactt attgcagtt tttttacaa gtttagtga 
      tgaggaaaa aaagaattt cttgcaaca cttgcaagg 
      tttggattt ggacgccag tgccaggtt gtttgaacg 
      agcgaagaa ttgtcttga cgtggctcc ggacaccga 
      agacatgaa gccgattcc gcattcgca gttcgagtc 
      ggaggaggg ttgtgacgg tgaaacgct cgaaaggta 
      cagaatacg ggggttgga ggcgatcgg cgccggtga 
      agtgggtgt cctgcttct ggcaggggg gcaaggcac 
      ccggctggg ggtcacgta ccccaaggg catgtactc 
      ggtcggctt gccctccgg gaaaaccat cttccaaat 
      ccaggccga acgcatcag acgggtcca acacttggc 
      taagaaaca cacagggaa gggtggcaa agtcacttg 
      gtatatcat gacaagtgg ccccactga taaaatgac 
      tgaaacatt cctcaaatc gcacaattt cttcggcct 
      tgaccccca aaacgtcgt tttgttcaa gcagggcct 
      actgccctg tttcgattt cgacgggaa gataatcct 
      agaggcccc gaatctagt tgccttagc cccggacgg 
      aaacggcgg gatataccg tgcgttgca cgtgaacgg 
      tgttttaga cgacatgcg gaggcgtgg cgtgaagta 
      cattcacgc ccacagcgt cgataacat cctcaccaa 
      agtcgccga cccggtttt cattggcta ctttattga 
      aaagggtgg ggattgcgc ggccaaagt ggtcaaaaa 
      ggcgggacc aacggaagc ggtgggcgt ggtgtgcca 
      aatcaaagg gcggttcca agtcgttga atacagcga 
      aatttcgga agaaaaggc gcatttgcg tgacgagga 
      ggggaatct ggtttatag tgctggcaa catttgcaa 
      ccatttatt cacaactgt gtttctaca aagggtcag 
      cgatgaatt cgaacacga attgaagtt acacgtggc 
      taagaaaaa aattccttt cgttgacga aaccggaca 
      aacagtcac accggaaaa acccaacgg aattaaaat 
      agaaaaatt cattttcga cgttttccc attcagtga 
      tcggttcgt gacgtggga agtgccccg taaaagtga 
      attcagtgc aatgaaaaa tatggatag tgtcggtaa 
      agactgccc ttccacggc acgccagga tttactagc 
      cttgcatcg gacttatat tgaaaaagc cgggggcgt 
      ggtttccgc agaggtgga gatttcgcc cctcctgtc 
      gtacacagg ggaggagct tgaagcccg cgtcaaagg 
      aaaaatgtt tacagaaac gaccgtttt attgggaga 
      ggaagagat tagtattaa tggtgggag ggcttaaac 
      tacgatttg atcaaaaag actgcttcc gtcccgcct 
      acttaatcg ctactttat gaaagacag tgcgcttta 
      ttttatgca caaagtcgc aacatatat gtaaagtat 
      ttccaattt tatataagg