Status
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Gene information
General information
TC031284
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005590,TC005592,TC005591
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10897189..10974386
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031284
    iBeetle screen iB_00893

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs femur fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs tibiotarsus fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus shape shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031284 RA
      MADLEAVLA DVSYLMAME KSKCTPAAR ASKKIVLPD 
      PSVRSVMHK YLEKKNEVN FDKIFHQTL GYLLFKDFC 
      ENLSEVPVP QLRFYEEIK SYEKLESAE ERMKQARDI 
      YDNFIMKEL LARSHDYSK ECLQHVQKH LMKNDVPVT 
      LFEPYIEEI YNHLRDEPF KKFLESEKY TRFCQWKNL 
      ELNIQLTMN DFSVHRIIG RGGFGEVYG CRKADTGKM 
      YAMKCLDKK RIKMKQGET LALNERIML SLVSTGQDC 
      PFIVCMTYA FHTPDKLCF ILDLMNGGD LHYHLSQHG 
      VFNESEMKF YAAEVILGL EHMHRRYIV YRDLKPANI 
      LLDEHGHVR ISDLGLACD FSKKKPHAS VGTHGYMAP 
      EVLSKGTAY DSSADWFSF GCMLYKLLK GHSPFRQHK 
      TKDKHEIDR MTLTMNVEL PDSFSKELK SLLEGLLQR 
      DVDKRLGCK GNGADEVKE HPFFAGIDW QQVYLQKYT 
      PPLIPPRGE VNAADAFDI GSFDEEDTK GIKLTDADQ 
      ELYKNFPLV ISERWQNEV AETVFDTIN YEADRAENK 
      KKAKQKKLF DLDEKESDC ILHGYIKKL GGPWMSSWQ 
      MRYAKLYPN RLELHPDSA KPEMVFMDQ VEEVSPELV 
      QVKNENCIV VRMRDGKIV LTNTDVKSR LRELCFKSR 
      PKMSLSSTV VATAPFEGQ KPGTSGLRK KVKVFMEKN 
      YTENFVQCI LDALGPKLK GATLVVGGD GRYFSKQAI 
      NIIIRIAAA NQVARLIIG ERGILSTPA VSTLIRTHK 
      VLGGIVLTA SHNPGGIRH DFGIKFNIE NGGPAPDSF 
      TNAVYELTK KITEYKFTP DLDCEFDKK GVQKFTVDG 
      REFVVEVVD SSDDYVALM KEIFDFGKL KDLIRGNGK 
      RPPFQVLID SMNGVTGVY VSRIFVEEL GASPDNNVR 
      RIIPLDNFG EIHPDPNLT YAKDLVDKV KEDTTYDLG 
      AAFDGDGDR NMIIGKNAF FVTPSDSLA VIANNLECI 
      PYFQKRGVH GFARSMPTA AAVDRVAAK LGKEMFEVP 
      TGWKYFGNL MDAGRLSLC GEESFGTGS DHIREKDGI 
      WAVLAWLSI IAHKNMSVE DILTEHWKI YGRNYFTRY 
      DYEECNTDD ANKMMEHLE KTIADSGFV GKVFTAGGK 
      SYKVKIADN FSYIDPIDK SCAKNQGIR VLFEDGSRV 
      IYRLSGTGS TGATIRLYI DSYEKDNVT SDAQDEIGL 
      KEWLTSLKS AHKLSQELL GSMAKKAGK IYGTDSNNK 
      NAIIAAHRN TNGN
    CDS sequence
    • >TC031284 RA
      atggcggac ttagaggca gtgctggcc gatgtcagc 
      tatctcatg gccatggag aagagcaag tgcacacca 
      gctgccagg gccagcaag aagatcgtg ctgcccgac 
      cccagcgtt cgcagcgtg atgcacaag tatctggag 
      aagaagaac gaggtgaac tttgacaag atcttccat 
      caaacatta ggctatctg ttatttaag gatttttgc 
      gagaatttg tcggaagtt cccgtgcct cagcttcgc 
      ttttatgaa gagataaaa tcgtacgaa aagctagaa 
      agtgccgag gagaggatg aagcaagcg cgcgacatc 
      tacgacaac ttcatcatg aaggagctc ctggcgcgg 
      tctcacgac tactccaag gagtgcctc cagcacgtc 
      cagaagcac ctaatgaag aacgacgtt cccgtcaca 
      ctgtttgag ccttacata gaagaaatt tataaccat 
      ttaagggac gaaccgttc aagaaattt ttagaaagc 
      gaaaaatat acgaggttt tgccaatgg aaaaatttg 
      gaactgaac atacaactg actatgaac gacttcagc 
      gtgcacagg ataataggc aggggcggc ttcggggag 
      gtttacggt tgtcggaag gccgacacg ggcaaaatg 
      tacgcgatg aaatgtcta gacaagaaa aggatcaaa 
      atgaaacaa ggggagact ttggcgtta aacgaacgc 
      attatgttg tcccttgta agcacagga caggattgt 
      ccgtttata gtttgtatg acgtatgcg tttcacacg 
      cccgataag ctctgtttc atattagat ttaatgaat 
      ggcggcgat ttgcattat catttgagc caacacggc 
      gtcttcaac gagtctgaa atgaaattc tacgcggcc 
      gaagtgata ttaggtttg gaacatatg cataggagg 
      tatattgta tatagagat ttaaaacct gcaaatatt 
      ctattagac gaacatggc catgttcgg atatctgat 
      ttaggtcta gcctgtgat ttttcaaaa aagaaaccc 
      cacgccagt gtaggaact cacggttac atggcaccg 
      gaagtgctc tcaaaaggc acagcgtac gattccagt 
      gccgactgg ttcagtttc ggatgtatg ttgtacaaa 
      ttattgaaa ggacattcg ccgttccgc caacacaaa 
      accaaagac aagcacgaa atcgacagg atgacgcta 
      acaatgaac gtcgaactt cccgattcg tttagcaaa 
      gaattgaag tcgctcctc gagggttta ctccagcga 
      gacgtcgac aaaagactg ggttgcaaa ggcaacggg 
      gccgacgag gtcaaggag caccccttt ttcgccggc 
      atcgactgg cagcaggtc tacctccag aagtacacc 
      cctccttta attccccca aggggcgag gtgaacgcg 
      gcggacgcc ttcgacatc ggctccttc gacgaggag 
      gacaccaag gggatcaag ctcacggac gccgaccag 
      gaactctac aaaaacttc cccttggtg atttccgag 
      cggtggcaa aacgaagtc gccgaaacc gtcttcgac 
      acgatcaac tacgaggcc gaccgagcc gaaaacaag 
      aaaaaagcc aaacagaag aaactcttc gacctggac 
      gaaaaagaa tcggactgc attttgcac ggctatatt 
      aagaaatta ggcgggccg tggatgtcc agctggcag 
      atgaggtat gcaaagctg taccccaac aggttggaa 
      ctgcacccg gattcggcg aaaccggaa atggtcttc 
      atggatcaa gtcgaggaa gttagtccc gagctggtg 
      caagtgaaa aatgaaaat tgcatcgtt gtcaggatg 
      agggatggg aagattgtc ctcacgaat acggacgtt 
      aaaagccga ttacgagag ttgtgtttc aaaagccga 
      ccgaaaatg agcctctct tcgaccgtg gtggccaca 
      gcccccttc gagggccag aagccgggc acgagcggc 
      ctccggaag aaagtcaaa gttttcatg gagaaaaac 
      tacacggag aatttcgtg cagtgcatt ttggacgct 
      ttggggccc aagctcaag ggggcgacg ttggtggtg 
      ggcggcgac ggccggtat ttctccaaa caggcgatc 
      aatattatc attcggatt gcggcagct aatcaggtg 
      gctagactg ataattggg gaacggggg attttgtca 
      accccggcg gtttcgacg ctcattcgc acgcataaa 
      gtcttgggc gggattgtc ttaactgcc tcacataat 
      ccgggcgga attaggcac gattttggt attaaattt 
      aatatagaa aatgggggc cctgccccg gattcgttc 
      acgaacgct gtgtacgaa ttgacgaag aaaatcacg 
      gagtataag ttcaccccc gacttggat tgcgaattt 
      gataaaaag ggggtgcag aagtttacc gttgatggg 
      agggagttt gtggtggaa gttgtggat tcgtccgat 
      gattatgtg gcgttaatg aaggagatt ttcgatttt 
      gggaaattg aaagatttg attcggggg aatgggaaa 
      aggccgccg ttccaggtt ttgattgat tcaatgaat 
      ggggtgact ggagtttac gtcagccgg attttcgtc 
      gaagaactc ggtgccagc cctgataac aacgtccgt 
      cgaatcatc ccactagat aatttcggg gaaatacat 
      cccgaccct aatctcacc tatgccaaa gacttggtc 
      gacaaggtc aaagaggac accacgtac gaccttggc 
      gccgccttc gatggcgat ggggaccgt aacatgata 
      attgggaag aacgccttt ttcgtgacg cctagcgac 
      agcttagct gtcattgcc aataacctc gagtgcatc 
      ccctatttc caaaaacga ggcgtgcac ggatttgcc 
      cgatcgatg ccgaccgcg gccgccgtt gaccgagtg 
      gctgcgaaa ttggggaag gaaatgttc gaggttccc 
      acgggttgg aagtatttc gggaatttg atggatgcg 
      gggcgcctg tctctatgt ggggaggaa agtttcggg 
      accggctct gaccatata agggagaaa gacgggatt 
      tgggccgtt ctggcgtgg ttgtccatc attgcacat 
      aagaatatg agtgttgaa gatattttg accgaacat 
      tggaaaatc tacggtcgg aattatttc acgcgttac 
      gattacgag gagtgcaat actgacgat gcgaacaaa 
      atgatggaa catttggaa aaaacaatc gccgattcg 
      ggttttgtt gggaaggtg tttacggcg ggtggaaag 
      tcgtataaa gttaaaatt gccgataat ttttcgtat 
      attgatccg atcgataaa agttgtgcc aaaaatcag 
      ggaattagg gttttgttt gaagacggt tccagggtg 
      atttatcgg ttgtcgggg actgggagt acaggcgcc 
      acgattaga ctgtatatt gacagttat gagaaggat 
      aacgtcact tccgatgca caagacgaa ataggccta 
      aaagagtgg cttacgtcc ctgaagtct gctcacaag 
      ttgtctcag gagctgttg gggtcgatg gcgaagaaa 
      gccggcaag atctacggg acggactcg aacaataaa 
      aatgcgata atagcggca catcgtaac acaaacggt 
      aactaa
    mRNA sequence
    • >TC031284 RA
      aatcagtcg ctgtttgag tttcggcgg tcgcgatag 
      ggacttgag ttccctcct tgatgatcc agggacgga 
      actttgaga ttgctcact cgacggcgc gttcctcgt 
      cagtctcct gtgccgcga ttattatgt aaagtcaat 
      gagggccgt tatgtaagg acgagtgcg gggcgcgtg 
      cggtaggtg ttggtgagc tgtgcacag tgtaccgaa 
      atatgttta tttgagcga gtgtggtgc aaactgcaa 
      aatggcgga cttagaggc agtgctggc cgatgtcag 
      ctatctcat ggccatgga gaagagcaa gtgcacacc 
      agctgccag ggccagcaa gaagatcgt gctgcccga 
      ccccagcgt tcgcagcgt gatgcacaa gtatctgga 
      gaagaagaa cgaggtgaa ctttgacaa gatcttcca 
      tcaaacatt aggctatct gttatttaa ggatttttg 
      cgagaattt gtcggaagt tcccgtgcc tcagcttcg 
      cttttatga agagataaa atcgtacga aaagctaga 
      aagtgccga ggagaggat gaagcaagc gcgcgacat 
      ctacgacaa cttcatcat gaaggagct cctggcgcg 
      gtctcacga ctactccaa ggagtgcct ccagcacgt 
      ccagaagca cctaatgaa gaacgacgt tcccgtcac 
      actgtttga gccttacat agaagaaat ttataacca 
      tttaaggga cgaaccgtt caagaaatt tttagaaag 
      cgaaaaata tacgaggtt ttgccaatg gaaaaattt 
      ggaactgaa catacaact gactatgaa cgacttcag 
      cgtgcacag gataatagg caggggcgg cttcgggga 
      ggtttacgg ttgtcggaa ggccgacac gggcaaaat 
      gtacgcgat gaaatgtct agacaagaa aaggatcaa 
      aatgaaaca aggggagac tttggcgtt aaacgaacg 
      cattatgtt gtcccttgt aagcacagg acaggattg 
      tccgtttat agtttgtat gacgtatgc gtttcacac 
      gcccgataa gctctgttt catattaga tttaatgaa 
      tggcggcga tttgcatta tcatttgag ccaacacgg 
      cgtcttcaa cgagtctga aatgaaatt ctacgcggc 
      cgaagtgat attaggttt ggaacatat gcataggag 
      gtatattgt atatagaga tttaaaacc tgcaaatat 
      tctattaga cgaacatgg ccatgttcg gatatctga 
      tttaggtct agcctgtga tttttcaaa aaagaaacc 
      ccacgccag tgtaggaac tcacggtta catggcacc 
      ggaagtgct ctcaaaagg cacagcgta cgattccag 
      tgccgactg gttcagttt cggatgtat gttgtacaa 
      attattgaa aggacattc gccgttccg ccaacacaa 
      aaccaaaga caagcacga aatcgacag gatgacgct 
      aacaatgaa cgtcgaact tcccgattc gtttagcaa 
      agaattgaa gtcgctcct cgagggttt actccagcg 
      agacgtcga caaaagact gggttgcaa aggcaacgg 
      ggccgacga ggtcaagga gcacccctt tttcgccgg 
      catcgactg gcagcaggt ctacctcca gaagtacac 
      ccctccttt aattccccc aaggggcga ggtgaacgc 
      ggcggacgc cttcgacat cggctcctt cgacgagga 
      ggacaccaa ggggatcaa gctcacgga cgccgacca 
      ggaactcta caaaaactt ccccttggt gatttccga 
      gcggtggca aaacgaagt cgccgaaac cgtcttcga 
      cacgatcaa ctacgaggc cgaccgagc cgaaaacaa 
      gaaaaaagc caaacagaa gaaactctt cgacctgga 
      cgaaaaaga atcggactg cattttgca cggctatat 
      taagaaatt aggcgggcc gtggatgtc cagctggca 
      gatgaggta tgcaaagct gtaccccaa caggttgga 
      actgcaccc ggattcggc gaaaccgga aatggtctt 
      catggatca agtcgagga agttagtcc cgagctggt 
      gcaagtgaa aaatgaaaa ttgcatcgt tgtcaggat 
      gagggatgg gaagattgt cctcacgaa tacggacgt 
      taaaagccg attacgaga gttgtgttt caaaagccg 
      accgaaaat gagcctctc ttcgaccgt ggtggccac 
      agccccctt cgagggcca gaagccggg cacgagcgg 
      cctccggaa gaaagtcaa agttttcat ggagaaaaa 
      ctacacgga gaatttcgt gcagtgcat tttggacgc 
      tttggggcc caagctcaa gggggcgac gttggtggt 
      gggcggcga cggccggta tttctccaa acaggcgat 
      caatattat cattcggat tgcggcagc taatcaggt 
      ggctagact gataattgg ggaacgggg gattttgtc 
      aaccccggc ggtttcgac gctcattcg cacgcataa 
      agtcttggg cgggattgt cttaactgc ctcacataa 
      tccgggcgg aattaggca cgattttgg tattaaatt 
      taatataga aaatggggg ccctgcccc ggattcgtt 
      cacgaacgc tgtgtacga attgacgaa gaaaatcac 
      ggagtataa gttcacccc cgacttgga ttgcgaatt 
      tgataaaaa gggggtgca gaagtttac cgttgatgg 
      gagggagtt tgtggtgga agttgtgga ttcgtccga 
      tgattatgt ggcgttaat gaaggagat tttcgattt 
      tgggaaatt gaaagattt gattcgggg gaatgggaa 
      aaggccgcc gttccaggt tttgattga ttcaatgaa 
      tggggtgac tggagttta cgtcagccg gattttcgt 
      cgaagaact cggtgccag ccctgataa caacgtccg 
      tcgaatcat cccactaga taatttcgg ggaaataca 
      tcccgaccc taatctcac ctatgccaa agacttggt 
      cgacaaggt caaagagga caccacgta cgaccttgg 
      cgccgcctt cgatggcga tggggaccg taacatgat 
      aattgggaa gaacgcctt tttcgtgac gcctagcga 
      cagcttagc tgtcattgc caataacct cgagtgcat 
      cccctattt ccaaaaacg aggcgtgca cggatttgc 
      ccgatcgat gccgaccgc ggccgccgt tgaccgagt 
      ggctgcgaa attggggaa ggaaatgtt cgaggttcc 
      cacgggttg gaagtattt cgggaattt gatggatgc 
      ggggcgcct gtctctatg tggggagga aagtttcgg 
      gaccggctc tgaccatat aagggagaa agacgggat 
      ttgggccgt tctggcgtg gttgtccat cattgcaca 
      taagaatat gagtgttga agatatttt gaccgaaca 
      ttggaaaat ctacggtcg gaattattt cacgcgtta 
      cgattacga ggagtgcaa tactgacga tgcgaacaa 
      aatgatgga acatttgga aaaaacaat cgccgattc 
      gggttttgt tgggaaggt gtttacggc gggtggaaa 
      gtcgtataa agttaaaat tgccgataa tttttcgta 
      tattgatcc gatcgataa aagttgtgc caaaaatca 
      gggaattag ggttttgtt tgaagacgg ttccagggt 
      gatttatcg gttgtcggg gactgggag tacaggcgc 
      cacgattag actgtatat tgacagtta tgagaagga 
      taacgtcac ttccgatgc acaagacga aataggcct 
      aaaagagtg gcttacgtc cctgaagtc tgctcacaa 
      gttgtctca ggagctgtt ggggtcgat ggcgaagaa 
      agccggcaa gatctacgg gacggactc gaacaataa 
      aaatgcgat aatagcggc acatcgtaa cacaaacgg 
      taactaaat ttgtggagg atcaaacct tgtcctgca 
      atgaaatag ccgatgcgg gctacaagc atttcgcga 
      agatttatc gtttcttgc aatgtctta ggcactatt 
      gttgttgct ccctatttt atttgttag gttttttat 
      gttttaata tacagttaa gaggaataa ccgattatt 
      tatcgacgc aacatttat atattgaga tgcgttgcg 
      gtcttgtaa taacttaaa ttcatgaca aagtgtatt 
      gtattattg ttatttata ttatttatg ccactagat 
      tttgctttg agcaggtac ttgttataa cggtagttt 
      atcaccttt tttgaatat atttattga ggctgttct 
      gggaatgtg gcgaaaaaa aggagcgca cattccact 
      cgattttca caaatctag tcaagcaca ggataacaa 
      aatacaatc aaaaccatt tttgagggc tgttcaagc 
      gcattttca ggacagttt cgttattat ttttgattc 
      tgcttttta tttattcag ttggaaaaa tttgttcaa 
      gctcggggt agtcgcgct ttacagatt tttttaacg 
      acactgtat attcctgaa cagagatgt tcgtctcac 
      tggtaccaa caccaagct aataagtca ctagaatag 
      cttgtatgc cagctgcac tatctgtaa atacttgct 
      tgtttctta ttacagtta agttcacct agtacataa 
      aacgtgaaa caccctttg tgtggtgtg gactaagtt 
      gtgatttgt tctgcactc tataatttc gtgaagtaa 
      tcaaatatt ttgtacatt tttgtaaat aattcggtt 
      tagtttcat gtgaactag tgtatgtca cgttcagtt 
      gagagattc catctcgta aatgtagaa gaattttct 
      tccatctca atgtcaaaa tgcttcgat tcttttgag 
      cggaacttc aagtgtaaa aaggtgttt tttgatgat 
      ttttatttt catatgcgc taggccttt actaggtta 
      actgtcgtc gagcagcgt cagtccttt ttaaaatac 
      ttcactgcc tacagcgcg aatctccgg tcatatttt 
      aaaaacgac tgatgcgcc ctgaattgt gcgtggaaa 
      aacatgtac atttttgta tagcgtgtg atttagatg 
      tttgtgcca ttgtatccg gattttttt aacttcaac 
      cagatggca aaggtttcg tcggtcttg ccgccacca 
      tcagggtac cgcgccggt tggcagtcg cggaattca 
      ataaacagt gccaaatag c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY