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Gene information
General information
TC005583 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3950685..3955478
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005583
    iBeetle screen iB_00890
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005583 RA
      MSDSVQYFE GVEKLLEIW FTTSNSNSK TADLRKIPR 
      AKLESLLKI VRCEIISFM KNEQVDAYV LSESSMFIS 
      KRRFILKTC GTTTPLLCL EPLLLLAEQ YAGFTEVED 
      LFYSRKNYK RPELQVTPH KHFDQEVAL LDSLFPDGV 
      AYCMGAVNK DCWYLYTLN PFPRRTPHQ PLPAVKEEP 
      DQTLEILMT DLDPEIMSI FSKEESLNA TEATKRSGI 
      DKIIPNMII DDFLFEPCG YSMNGVTKN GCYMTIHIT 
      PEPEFSYVS FETNVPCSS YREIISKVL DTFLPGKFT 
      VTVFANQAS PTKDTAEEF QYLSSIGEW SRRDIQHCQ 
      FKNYDLIYT FYSKFPS
    CDS sequence
    • >TC005583 RA
      atgagtgac agtgttcaa tacttcgaa ggagttgag 
      aaactcctc gagatctgg tttacgacg agcaacagc 
      aacagcaaa acggccgac ttgcgaaaa ataccaagg 
      gcgaaactc gagtcgctt ttgaaaatt gtgaggtgc 
      gaaatcatc agttttatg aaaaacgaa caagtggat 
      gcctacgtg ctcagcgaa agttcaatg ttcatttcg 
      aaacgtcgt tttattttg aaaacttgc ggcacgacg 
      acgccgctt ttatgcctg gagcctctc ctacttctg 
      gccgaacaa tacgccgga tttaccgaa gtcgaagac 
      ctcttctac tcgaggaaa aactacaaa agacccgaa 
      ctccaagta accccacac aagcatttc gatcaggaa 
      gtggcgctc ttggactcg ctgtttccg gacggggtg 
      gcctattgt atgggggcg gttaacaag gattgctgg 
      tacttgtac actttgaac cccttcccg agacgaacc 
      ccccatcag ccgcttccc gcagtcaag gaggagccc 
      gaccagact ttggagatc ttgatgact gacttggac 
      cccgaaatt atgagtatt ttctcgaag gaagagtcg 
      ctgaatgcg accgaagcc acaaagagg tccgggatc 
      gataagatc attcctaac atgataatc gatgatttt 
      ttgttcgaa ccgtgcgga tactcgatg aacggagtc 
      acgaaaaat ggctgttat atgacgatc catatcacg 
      cccgagccg gagttttcg tacgtcagt ttcgagacg 
      aacgttcct tgtagctcc tacagggag atcatcagc 
      aaagtgttg gacacgttc ctgccgggc aagttcaca 
      gtgacggtg ttcgccaat caggcgtcg ccaactaag 
      gacacggcc gaggaattc cagtacttg agctcgatt 
      ggcgagtgg tcgcgtcgc gacatccag cattgccag 
      ttcaaaaac tacgatctt atctacacc ttctactcg 
      aagttcccg agctga
    mRNA sequence
    • >TC005583 RA
      attcattgt tgacaaatg cgcgcgcaa tttgttctg 
      ccggcatcg agctcgaat tgaattttt atcttcatt 
      agaattcga gcgagagaa gcgaccgga cgtcccggc 
      ttaaaaatg agtgacagt gttcaatac ttcgaagga 
      gttgagaaa ctcctcgag atctggttt acgacgagc 
      aacagcaac agcaaaacg gccgacttg cgaaaaata 
      ccaagggcg aaactcgag tcgcttttg aaaattgtg 
      aggtgcgaa atcatcagt tttatgaaa aacgaacaa 
      gtggatgcc tacgtgctc agcgaaagt tcaatgttc 
      atttcgaaa cgtcgtttt attttgaaa acttgcggc 
      acgacgacg ccgctttta tgcctggag cctctccta 
      cttctggcc gaacaatac gccggattt accgaagtc 
      gaagacctc ttctactcg aggaaaaac tacaaaaga 
      cccgaactc caagtaacc ccacacaag catttcgat 
      caggaagtg gcgctcttg gactcgctg tttccggac 
      ggggtggcc tattgtatg ggggcggtt aacaaggat 
      tgctggtac ttgtacact ttgaacccc ttcccgaga 
      cgaaccccc catcagccg cttcccgca gtcaaggag 
      gagcccgac cagactttg gagatcttg atgactgac 
      ttggacccc gaaattatg agtattttc tcgaaggaa 
      gagtcgctg aatgcgacc gaagccaca aagaggtcc 
      gggatcgat aagatcatt cctaacatg ataatcgat 
      gattttttg ttcgaaccg tgcggatac tcgatgaac 
      ggagtcacg aaaaatggc tgttatatg acgatccat 
      atcacgccc gagccggag ttttcgtac gtcagtttc 
      gagacgaac gttccttgt agctcctac agggagatc 
      atcagcaaa gtgttggac acgttcctg ccgggcaag 
      ttcacagtg acggtgttc gccaatcag gcgtcgcca 
      actaaggac acggccgag gaattccag tacttgagc 
      tcgattggc gagtggtcg cgtcgcgac atccagcat 
      tgccagttc aaaaactac gatcttatc tacaccttc 
      tactcgaag ttcccgagc tgagggtgt caggtaaag 
      ttgtacttt tgtgcagct gattgtatc acccccatc 
      ctcgcctcc tttctcctc tcttccatt gtctcagtc 
      gtcttcctt gaagtctcc tcgcgtcgt ctgtttcaa 
      aagtacaaa tctgcatcg agagggtct cgacatcat 
      cgctttgga ttcgacgtt ttcgcccca tttcttgta 
      ctgtttttt aattcgttt gctccggtt ttcacgttc 
      acagcgttc gagtcgcca ctggtcttg ttttcgtgg 
      tggcgtaac aagatttcg tgagaatta ctgaaaaac 
      ttttaaagc cagtagaga gcatagtgg acatatgtg 
      ttgttttta ccttttacc tatgagggt taggagaaa 
      catgcatgg aaagttttt agtttttca gctgtccaa 
      cactatgtc gatgctgct ttaagagtt tcttctgat 
      ttctttatt acaaatttt aagctagag tggccactg 
      gcactttct atttaccta atatacgcg gaccttttg 
      ggaggatat tcgacctgt gtttatatg tacattttt 
      tcgatgtcg aaattgctg ttttacaag cagggttgc 
      aaaggtcca ttcaaaaag taagctatt gtaacttga 
      tgctgatcc aataacctg tcagcactt gtaggtgca 
      taatagctt attttttac atattgaga atatcttat 
      ttttttaaa tagaacata gactttttt attctttat 
      ttttacatt acttactta attacgtag actactaat 
      ttaaactta aggggtaga gtagatttt aatcttgac 
      atactataa aaatgttga tgtttaaga taatttaaa 
      gtcaataaa gttaacatt attca