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Gene information
General information
TC005582 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3955679..3960668
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005582
    iBeetle screen iB_00889
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature slightly fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005582 RA
      MAQVKIDEE LKGGKIRRS NVCRQLRVL AVIMLSFIR 
      YYIDLLIDK VFGLYYNSR VQRVEKPPS KIVLESATS 
      LARKIRKRE LKSEEVVRA FIDRVHQVN KLLNSVVDE 
      RFDEAIEDA QNLDKDIAD GKITEKDFD KKPFLGIPF 
      TTKESTACK GLSNTFGLL NRRLQKAAF DAQVVQEMK 
      NAGGILIGV TNVPQLNLW QETFNPVYG VTNNPYNTT 
      RNVGGSSGG EASIIAACG SPIGIGTDI GGSLRIPAF 
      MCGVFAHKP TSGLISTHG LTFRTGKEQ ETMVVVGPM 
      AKYSEDLTP FLKVLLGEN SAKLKLDQS VDVAKIRVY 
      YVTDPKDPF VSPFRDEMN KAMLKVIRH FAEILPEKP 
      EMVNIQELK YGGKLWRYW MTQEPNTNF NLDLGNRET 
      EVNSVIELL KFCIRISDY NIAVMLNLV NGLLPAENA 
      EWVREITDT LHKKLTSIL GTSGVLIYP SAPFPASYH 
      YSAVLRPWN MNLFGIWNA LKFPVTQVP LGLGQEGLP 
      LGVQVVAAP FQDHLAIAV AKELEKTFG GYVPPF
    CDS sequence
    • >TC005582 RA
      atggctcag gtgaagata gacgaggag ctcaaaggt 
      ggtaaaatc cggcgtagc aacgtgtgt aggcagttg 
      cgagtactc gcagtaatc atgctctcg tttataagg 
      tattacatt gatctcctg attgacaaa gtgttcggg 
      ttgtactac aactcgcgc gtgcagcgc gtggagaag 
      cccccgagt aaaatcgtg ctggagagc gccacctcc 
      ctggcccgc aagatccgc aaacgcgag ctaaagtcc 
      gaggaggtc gtccgtgcc ttcatcgac cgcgtccac 
      caagtgaac aagctcctc aactcggtg gtggacgag 
      cgcttcgac gaggcgatc gaagacgcc cagaacctg 
      gacaaagac atcgccgat gggaaaatc accgagaaa 
      gattttgac aaaaagcca tttctcggg atacctttt 
      accacaaag gaatcgaca gcttgcaaa ggtctctcc 
      aacacgttc ggcttgctc aaccgccgg ctccagaag 
      gccgcattc gatgcccaa gttgtgcaa gagatgaaa 
      aacgccgga gggatactg atcggggtc accaacgtg 
      ccccagctc aacctgtgg caggagacc ttcaacccc 
      gtgtacggc gtcaccaac aacccctac aacaccacg 
      cggaacgtg gggggctcc tcggggggc gaggccagc 
      attatcgca gcctgcggc tctccgatc ggcatcggg 
      acggatata gggggctcc ctgcggatc ccggccttc 
      atgtgcggg gttttcgcc cacaagccc acctcgggc 
      ctgatctcg acacatggg ctcacgttc cggaccggc 
      aaggagcag gagacgatg gtggtggtg gggcccatg 
      gccaagtac agtgaggac ttaaccccg tttttgaaa 
      gtcttacta ggcgaaaac tccgccaaa ttaaagcta 
      gatcaaagt gttgacgtg gccaaaatc agggtctat 
      tacgtcact gacccaaaa gaccccttc gtgagcccc 
      ttccgggac gagatgaac aaagcaatg ctgaaagtg 
      atcaggcat tttgccgaa attttgccc gaaaagccc 
      gaaatggtc aacattcag gagctgaaa tatgggggg 
      aagctttgg cggtattgg atgacacag gagccaaac 
      actaatttt aaccttgat ctcgggaat cgcgaaaca 
      gaagttaat agcgtgatt gaattattg aaattttgt 
      attagaata agtgactat aacatcgcg gttatgcta 
      aacctagtt aatggcctc ctcccggct gagaatgcc 
      gaatgggtg agagaaatc actgacact ctccataaa 
      aaattaacg tcgattttg ggcaccagt ggggtgctg 
      atttacccc tcggccccc ttcccggca agttatcac 
      tatagcgcg gtcttgaga ccttggaat atgaatttg 
      tttgggatt tggaacgca ttgaagttc cccgttacg 
      caggttcct ttggggctg ggccaggag ggcctgccg 
      ttgggggtg caagtggtg gcagccccc tttcaggac 
      cacctagct attgccgta gctaaggaa ttggagaag 
      acgtttggc ggatacgtg ccgcccttt taa
    mRNA sequence
    • >TC005582 RA
      cgagcattt agcggggcc gagcggggt cgatatcct 
      acggctgaa aggggagtg tctgttctg taacatata 
      atttcagat taacaacaa aaaaacacc aatttggtc 
      aaaacattc acgcaaatg aaacacgta agtcaagct 
      ttgttagtt tttattaac atcaaataa cttaatatc 
      acactaaag gaaaattat ctactattt acacaaaac 
      tagaatttt aaatattga atccagcct tcctcatgc 
      actccttgt gggcttcta ttaagtgtt tgcaattct 
      cttccccat tttcaatga tactgaaac aaacagctc 
      agtttgggg caattcgca aaaaataag ttaccaagc 
      gtctcgcac ttttttcgt ctcgggaca agcacagca 
      tggtttcaa tttcttcgg ctcctcttt agcccccgg 
      ctgttgcgt ggaagccgc ctcgacttt ctcactcac 
      gttccccat tcttgtcta tcaaacaat tagctaatg 
      accacgaat tatgcaata ttagtgcga ttatttcgc 
      aattacttg aaatttgca ccgaaatta tttaaccca 
      cctaacctc acttgtgcg acagctgac aagtggtgc 
      gcatgcacc tctccacgt aaaaaatgt caaaacatt 
      ccgtgcctg ttgttttgg tacgaaagt gctgaatat 
      tcaaaaaaa aataatgaa ttatgtatt gagctgtgg 
      agtataagt gtctggaga ttaattagt tgttgcgtt 
      aattgggtt aagatggct caggtgaag atagacgag 
      gagctcaaa ggtggtaaa atccggcgt agcaacgtg 
      tgtaggcag ttgcgagta ctcgcagta atcatgctc 
      tcgtttata aggtattac attgatctc ctgattgac 
      aaagtgttc gggttgtac tacaactcg cgcgtgcag 
      cgcgtggag aagcccccg agtaaaatc gtgctggag 
      agcgccacc tccctggcc cgcaagatc cgcaaacgc 
      gagctaaag tccgaggag gtcgtccgt gccttcatc 
      gaccgcgtc caccaagtg aacaagctc ctcaactcg 
      gtggtggac gagcgcttc gacgaggcg atcgaagac 
      gcccagaac ctggacaaa gacatcgcc gatgggaaa 
      atcaccgag aaagatttt gacaaaaag ccatttctc 
      gggatacct tttaccaca aaggaatcg acagcttgc 
      aaaggtctc tccaacacg ttcggcttg ctcaaccgc 
      cggctccag aaggccgca ttcgatgcc caagttgtg 
      caagagatg aaaaacgcc ggagggata ctgatcggg 
      gtcaccaac gtgccccag ctcaacctg tggcaggag 
      accttcaac cccgtgtac ggcgtcacc aacaacccc 
      tacaacacc acgcggaac gtggggggc tcctcgggg 
      ggcgaggcc agcattatc gcagcctgc ggctctccg 
      atcggcatc gggacggat atagggggc tccctgcgg 
      atcccggcc ttcatgtgc ggggttttc gcccacaag 
      cccacctcg ggcctgatc tcgacacat gggctcacg 
      ttccggacc ggcaaggag caggagacg atggtggtg 
      gtggggccc atggccaag tacagtgag gacttaacc 
      ccgtttttg aaagtctta ctaggcgaa aactccgcc 
      aaattaaag ctagatcaa agtgttgac gtggccaaa 
      atcagggtc tattacgtc actgaccca aaagacccc 
      ttcgtgagc cccttccgg gacgagatg aacaaagca 
      atgctgaaa gtgatcagg cattttgcc gaaattttg 
      cccgaaaag cccgaaatg gtcaacatt caggagctg 
      aaatatggg gggaagctt tggcggtat tggatgaca 
      caggagcca aacactaat tttaacctt gatctcggg 
      aatcgcgaa acagaagtt aatagcgtg attgaatta 
      ttgaaattt tgtattaga ataagtgac tataacatc 
      gcggttatg ctaaaccta gttaatggc ctcctcccg 
      gctgagaat gccgaatgg gtgagagaa atcactgac 
      actctccat aaaaaatta acgtcgatt ttgggcacc 
      agtggggtg ctgatttac ccctcggcc cccttcccg 
      gcaagttat cactatagc gcggtcttg agaccttgg 
      aatatgaat ttgtttggg atttggaac gcattgaag 
      ttccccgtt acgcaggtt cctttgggg ctgggccag 
      gagggcctg ccgttgggg gtgcaagtg gtggcagcc 
      ccctttcag gaccaccta gctattgcc gtagctaag 
      gaattggag aagacgttt ggcggatac gtgccgccc 
      ttttaaatg aatatgtgg gttttgggg ggttggggt 
      tttacatgt tgttatttt cctacttaa ttttattta 
      tacaactgt acgctttaa taaataaaa gacaagttt