Status
Please wait ...
Gene information
General information
TC005576 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11085075..11101671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005576
    iBeetle screen iB_00888

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005576 RA
      MGAQQCKRA EFRCNDGSC IASNKFCNG LQDCADGSD 
      EGHNCTPCN STYFGEIGR TYELEVKRP TEYQMPFLC 
      YLNFTAGGG VLGELVQLT FESFAVGTF ESFTTEGCP 
      DGFISIKEG NRPTSGGKW CGSAWGYTV YYSETPSVN 
      LTLRLERFP QQQSSGYTF EFKLSYKFL KLSDARIRY 
      GNATQRSYR GKLSSGSYC DRLLEGCSR RPCRIQSPN 
      YPGIYPRNV SCHYRVRER NVSPGKHAL IAVRQANFH 
      YKEHLPKFD NSDRVLRIW DQCNMMQDY IEILDGWGP 
      TATTLAHLC SGETVPEII SSGPELLVK FHTSPFGNP 
      FHPLPLSYL PGFELEVEV FYVNKESPT YIEHGKKCE 
      FLVTTFDNT SGLLESPLH SLPPNTTCH YHFRGHPKE 
      IIWISFIKY HVTNERLTD FNTGECDVQ LQIWDGDIK 
      SSTVPLIGQ FCKDDKPKL CDHSLLKNS SRLTRPCGL 
      AESYLSSGS DLTIAHSIR YGSVLYPVS FVLRYEFVD 
      LSQEGVQIA RNPCDRMLK EGAGRFYSP KITFLYGRG 
      GQEDLTCSY HFESDEQQR LKITFLRAG FGSKECVSV 
      YNPEIGRWV CGIKSYGNG FANLKIYEY PWTGVEILR 
      DCLCGNFSE PLTLITKTA RKVVVKFTV NGMKITEDY 
      RNYFFEANY EFVAGQSDG SCLSPWKSR RLKGSSGEI 
      SVRNNAQTL KQNEQSYQQ NQSNAFCLH QPWLIEPED 
      SLSNFIYLK IKGVNKRDS RLCPSKNRI LVYPAGRTE 
      DVRIICPYY ESIDDVVEM FSEGWNLYS YRKIQNKNS 
      RSFIIEFLQ REIGNFAVT WMEVSKNPA LTLPSTMLM 
      INPPDCPHS CPELGACIS SDLWCDGLR HCPSGNDEQ 
      ESNCSIHAG FPISYLNSV ALCALALVV TTTVIIVSF 
      YFFKYWRRE QKNVIVSVT EHTFLEFKS GLC
    CDS sequence
    • >TC005576 RA
      atgggggca caacagtgc aagagggcc gagttcagg 
      tgcaatgac ggcagctgc atcgccagc aataaattc 
      tgcaacggg ctgcaggac tgcgccgac ggcagcgac 
      gaagggcac aactgcacg ccttgcaac agcacctat 
      ttcggcgaa atcggccgc acttacgag ctggaagtg 
      aagaggccc actgaatac caaatgccc tttttgtgt 
      tatctcaat ttcacagct ggtggcggc gtgctgggc 
      gaactcgtt cagctcact tttgaatcg ttcgctgtt 
      ggaacgttc gagtcgttc accactgag ggatgcccg 
      gatggattt atttcaatt aaggaagga aatcggccc 
      acttcgggg ggcaaatgg tgcggaagc gcctggggc 
      tatacggtc tactacagc gaaacaccc agtgtcaac 
      ctgacgctg aggctcgaa cggttccct caacagcaa 
      agctctggc tacacgttc gagttcaag ctatcgtac 
      aaattcctg aaactgagc gacgcccgt atccgttac 
      ggcaacgca acgcagcgc agttaccgc ggcaaatta 
      agctcagga agttactgc gaccggcta ttggaaggc 
      tgctcacgt cgtccgtgt cgcatccaa tcccctaat 
      tatccgggg atttaccct cggaacgtc agctgtcat 
      tatcgagtc cgggaacgc aatgtttct cccggaaaa 
      cacgctctt atcgcagtc cgacaagcg aatttccac 
      tacaaggaa catttaccc aagtttgac aacagcgac 
      agagtattg cggatatgg gaccagtgc aacatgatg 
      caagattat atcgaaatt ttggacgga tggggaccg 
      actgccaca acactggca cacctgtgc agcggcgag 
      acagtcccg gaaattatc agcagcgga ccggaactg 
      ctagtcaag tttcacacc tcgcccttc gggaacccc 
      ttccaccca ctacccctg tcctatctg cccggattc 
      gagctggaa gtcgaggtc ttttacgtg aataaagaa 
      tccccgacg tacatcgaa catggtaaa aagtgcgaa 
      tttttggtt acgacattc gacaacacc tccggccta 
      ctcgaaagc cccctgcat tcgctcccg ccgaacacc 
      acgtgtcac taccacttc cggggccac ccgaaagaa 
      atcatctgg atcagtttt atcaagtac cacgtaacc 
      aacgaacgc ttgaccgac ttcaacacc ggcgagtgt 
      gacgtccaa ttacaaata tgggacggt gacatcaag 
      tcatccacc gtccccctg attggccaa ttctgcaag 
      gacgacaaa ccgaaactg tgcgaccac tctttattg 
      aaaaactcg tcacgtttg actcgccct tgcggcttg 
      gccgaaagt tacctatcg tcgggctct gatttaaca 
      atcgcgcat tcgattaga tacggcagt gtcttgtat 
      ccggtcagc ttcgtgctc cggtacgag ttcgtcgac 
      ttgtcgcaa gagggcgtg caaatcgcc cggaatccc 
      tgcgacagg atgctgaag gagggggct gggaggttc 
      tactccccc aagataacg ttcctgtac gggcgcggg 
      gggcaggag gacttgact tgcagttat cacttcgag 
      tcggacgag cagcaaagg ctcaaaata acgttcctg 
      agggccggg ttcgggagc aaggagtgc gtctcggtc 
      tacaatccc gagataggg cggtgggtt tgcgggatc 
      aagtcctac gggaacggg tttgctaat ttgaagatt 
      tacgaatat ccctggacc ggagtggag atcctgagg 
      gattgtctg tgcggtaat ttctccgaa cctttgaca 
      ctaattacg aaaactgcg cggaaagtc gttgtgaaa 
      ttcaccgta aatgggatg aaaatcacc gaggattac 
      aggaattac ttctttgaa gccaattat gaatttgtg 
      gcgggtcag agcgacggc agttgtctg agcccgtgg 
      aagagccgg cgcctgaag ggctccagt ggggagatt 
      tcggtgcgg aacaacgcc cagactttg aaacagaac 
      gaacagagc taccagcag aatcagtcg aacgcgttc 
      tgtctgcac cagccgtgg ctcatcgag ccggaggac 
      tccctcagc aacttcatc tacttgaaa atcaagggg 
      gtcaataag agggactcg cgcttgtgt ccgagcaaa 
      aaccggata cttgtgtac ccggctggg aggaccgaa 
      gacgtccga attatttgc ccctactat gaaagcatt 
      gacgatgtg gttgagatg ttttccgag ggctggaac 
      ctgtactcg taccggaag atacaaaat aagaactcg 
      cgcagcttc attattgaa tttctgcaa cgagagatc 
      ggcaatttt gccgtcacc tggatggaa gtctccaaa 
      aacccggcg ctgacgcta cccagcacc atgctgatg 
      atcaacccg cccgactgc ccccacagt tgtccggaa 
      ctgggggcc tgtatcagc tctgaccta tggtgcgac 
      ggcctgcgg cactgtccc tcaggcaac gacgagcaa 
      gaatccaac tgttcaatc cacgccggt ttccctata 
      tcatatctg aactctgtg gccctttgt gctctagct 
      ctggtagta acaactact gtaataatt gtaagtttc 
      tacttcttc aagtattgg agacgcgaa cagaaaaac 
      gttattgta tcagtaact gaacacacc tttctagag 
      tttaagagt ggtttgtgt taa
    mRNA sequence
    • >TC005576 RA
      tccgtaatc gttggcggc gtttaccat cccctcggg 
      cattgaaac tttgataaa agcaaagcc cagcttcga 
      tcgagcttc cgaattgcg cgtagcgat gcgcgccac 
      tcgcaagct ctgcccggc acctcaatg tttacagac 
      ttaatcggt ttattgccg cattttatt gttttatcg 
      acgctccga cgatgccgt cgacgcctc taaacaacc 
      tctagacgt tccgatgac gcgtaaaca agcataatg 
      ttctgggtt gtcgcgacc acgctgatc tgggccgtc 
      atgggggca caacagtgc aagagggcc gagttcagg 
      tgcaatgac ggcagctgc atcgccagc aataaattc 
      tgcaacggg ctgcaggac tgcgccgac ggcagcgac 
      gaagggcac aactgcacg ccttgcaac agcacctat 
      ttcggcgaa atcggccgc acttacgag ctggaagtg 
      aagaggccc actgaatac caaatgccc tttttgtgt 
      tatctcaat ttcacagct ggtggcggc gtgctgggc 
      gaactcgtt cagctcact tttgaatcg ttcgctgtt 
      ggaacgttc gagtcgttc accactgag ggatgcccg 
      gatggattt atttcaatt aaggaagga aatcggccc 
      acttcgggg ggcaaatgg tgcggaagc gcctggggc 
      tatacggtc tactacagc gaaacaccc agtgtcaac 
      ctgacgctg aggctcgaa cggttccct caacagcaa 
      agctctggc tacacgttc gagttcaag ctatcgtac 
      aaattcctg aaactgagc gacgcccgt atccgttac 
      ggcaacgca acgcagcgc agttaccgc ggcaaatta 
      agctcagga agttactgc gaccggcta ttggaaggc 
      tgctcacgt cgtccgtgt cgcatccaa tcccctaat 
      tatccgggg atttaccct cggaacgtc agctgtcat 
      tatcgagtc cgggaacgc aatgtttct cccggaaaa 
      cacgctctt atcgcagtc cgacaagcg aatttccac 
      tacaaggaa catttaccc aagtttgac aacagcgac 
      agagtattg cggatatgg gaccagtgc aacatgatg 
      caagattat atcgaaatt ttggacgga tggggaccg 
      actgccaca acactggca cacctgtgc agcggcgag 
      acagtcccg gaaattatc agcagcgga ccggaactg 
      ctagtcaag tttcacacc tcgcccttc gggaacccc 
      ttccaccca ctacccctg tcctatctg cccggattc 
      gagctggaa gtcgaggtc ttttacgtg aataaagaa 
      tccccgacg tacatcgaa catggtaaa aagtgcgaa 
      tttttggtt acgacattc gacaacacc tccggccta 
      ctcgaaagc cccctgcat tcgctcccg ccgaacacc 
      acgtgtcac taccacttc cggggccac ccgaaagaa 
      atcatctgg atcagtttt atcaagtac cacgtaacc 
      aacgaacgc ttgaccgac ttcaacacc ggcgagtgt 
      gacgtccaa ttacaaata tgggacggt gacatcaag 
      tcatccacc gtccccctg attggccaa ttctgcaag 
      gacgacaaa ccgaaactg tgcgaccac tctttattg 
      aaaaactcg tcacgtttg actcgccct tgcggcttg 
      gccgaaagt tacctatcg tcgggctct gatttaaca 
      atcgcgcat tcgattaga tacggcagt gtcttgtat 
      ccggtcagc ttcgtgctc cggtacgag ttcgtcgac 
      ttgtcgcaa gagggcgtg caaatcgcc cggaatccc 
      tgcgacagg atgctgaag gagggggct gggaggttc 
      tactccccc aagataacg ttcctgtac gggcgcggg 
      gggcaggag gacttgact tgcagttat cacttcgag 
      tcggacgag cagcaaagg ctcaaaata acgttcctg 
      agggccggg ttcgggagc aaggagtgc gtctcggtc 
      tacaatccc gagataggg cggtgggtt tgcgggatc 
      aagtcctac gggaacggg tttgctaat ttgaagatt 
      tacgaatat ccctggacc ggagtggag atcctgagg 
      gattgtctg tgcggtaat ttctccgaa cctttgaca 
      ctaattacg aaaactgcg cggaaagtc gttgtgaaa 
      ttcaccgta aatgggatg aaaatcacc gaggattac 
      aggaattac ttctttgaa gccaattat gaatttgtg 
      gcgggtcag agcgacggc agttgtctg agcccgtgg 
      aagagccgg cgcctgaag ggctccagt ggggagatt 
      tcggtgcgg aacaacgcc cagactttg aaacagaac 
      gaacagagc taccagcag aatcagtcg aacgcgttc 
      tgtctgcac cagccgtgg ctcatcgag ccggaggac 
      tccctcagc aacttcatc tacttgaaa atcaagggg 
      gtcaataag agggactcg cgcttgtgt ccgagcaaa 
      aaccggata cttgtgtac ccggctggg aggaccgaa 
      gacgtccga attatttgc ccctactat gaaagcatt 
      gacgatgtg gttgagatg ttttccgag ggctggaac 
      ctgtactcg taccggaag atacaaaat aagaactcg 
      cgcagcttc attattgaa tttctgcaa cgagagatc 
      ggcaatttt gccgtcacc tggatggaa gtctccaaa 
      aacccggcg ctgacgcta cccagcacc atgctgatg 
      atcaacccg cccgactgc ccccacagt tgtccggaa 
      ctgggggcc tgtatcagc tctgaccta tggtgcgac 
      ggcctgcgg cactgtccc tcaggcaac gacgagcaa 
      gaatccaac tgttcaatc cacgccggt ttccctata 
      tcatatctg aactctgtg gccctttgt gctctagct 
      ctggtagta acaactact gtaataatt gtaagtttc 
      tacttcttc aagtattgg agacgcgaa cagaaaaac 
      gttattgta tcagtaact gaacacacc tttctagag 
      tttaagagt ggtttgtgt taagaagaa ttaaaactt 
      caaatgact gacaatata tctgttgta ataaatgta 
      taaagtttt tatggtgaa taaaatcaa caaacttca 
      gcgaacatc tcttcccct gcaatcccc cggaggatg 
      ttatgggcg gtgacctcg gccatcccg ttcctggta 
      aattcggta gcactgccc aaatggggc aacaaaact 
      aaacccaat cagaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY