Status
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Gene information
General information
TC005572 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11123164..11127841
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005572
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00887

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005572 RA
      MASSENEGS VCQYTVEEI NYNEIQKLE AVGEGSFGV 
VYKGIWRDA FVAVKNIVS ENDKDAFIV EVRQLSRVK 
HENIVKLYG ACTKGPNIC LVMEYAEGG SLFNILHHA 
PNVHYTMAN AMSWLYQCA KGVEYLHGM KPKPLIHRD 
LKPPNLLLI NGGTLLKIC DFGTATDMT AIMTNNKGS 
AAWMAPEVF ETSNYTEKC DVFSWGIIL WEVLSRLKP 
FFHIKESGY PSAFMILWA KHSGRRPPL IRHCPPVIE 
KLMTQCWDQ EAAKRPSMK EIVKKMEVI CSLLPGADE 
PLQIRGGDD VVYKEEIEE FEDCVEEID QFPASTNGD 
NCVKSLPQP SAEMSRPLN IKISPDAWD LHDLDMQLR 
TMPGLDKMV PKNGSKNTT VEAGIESTK STPDGTDPN 
LDKVYLLLE PHLRPATPD ADNPQSLAL FEEHKQLAQ 
EYFKVQTEL AYVSQKKDK LLAAQSQEQ QQHTKLLKD 
LQAEKESLI LVRELLSRQ RENNNAARN NIDNWVLVP 
RDGTNTD
    CDS sequence
    • >TC005572 RA
      atggcgtct tcggaaaat gagggtagc gtatgtcag 
tacaccgtc gaggaaatc aactacaat gaaattcag 
aagcttgag gcagtcggt gagggcagc tttggggtc 
gtttacaag ggaatctgg cgcgatgcg ttcgtggcg 
gtcaaaaac atcgtctct gagaacgac aaagacgcc 
tttattgtg gaagtccgg cagctgtca agggtcaag 
catgagaac attgtcaag ctgtacggg gcctgcacc 
aagggcccc aacatctgc cttgtgatg gagtacgcc 
gaagggggc tccctcttc aatattcta caccacgca 
cctaacgta cactacacg atggccaac gccatgtcc 
tggctctac cagtgcgcc aaaggggtc gagtatctc 
catggcatg aaacccaaa cctttgata cacagagac 
ctcaaaccg cccaacttg ctcctgata aacgggggc 
accttgcta aagatatgc gactttggt actgccaca 
gacatgaca gcaatcatg accaacaac aaaggctcg 
gccgcgtgg atggccccc gaggtcttt gaaacgtca 
aactatacg gaaaagtgc gacgtcttc agctggggc 
ataatcctc tgggaggtg ttatcgcga cttaagcct 
tttttccac attaaggag tcgggctac ccttcggct 
ttcatgatc ttgtgggcg aagcacagc ggccggagg 
ccccctctc atcaggcac tgcccccca gtcatcgaa 
aaactgatg acacagtgc tgggatcag gaggcggcc 
aagcggccg agcatgaag gagattgtg aaaaaaatg 
gaagttatt tgctcgttg cttccgggc gccgacgag 
ccgttgcag atcaggggc ggtgacgat gtggtttac 
aaggaggaa atcgaagag tttgaggac tgtgtggag 
gaaatagac cagttcccc gccagcacc aacggcgat 
aattgtgtc aaatcgctg ccccagccg agtgccgag 
atgtcgcgg cccctcaac ataaaaata agccctgac 
gcatgggat ttacacgat ctcgacatg cagctgaga 
accatgccc ggccttgat aagatggta ccaaaaaac 
ggttccaaa aacacaacg gtagaggca ggcatcgag 
agcaccaag tcgacacct gacggcacc gatccaaat 
ttagataaa gtgtatctt ttattagag ccgcattta 
cgacctgcc actccagac gccgacaat ccgcagtcg 
ttggcgctg ttcgaagag cacaagcaa ctggctcag 
gaatatttc aaggtccaa actgagctc gcatatgtc 
tcgcaaaag aaggacaaa ctcctagcg gcgcagtcg 
caagaacag cagcagcac accaaactc ctcaaagac 
ctgcaagcg gagaaggag tccctgatc ttggtgcgg 
gagttgctc agtcggcaa agagaaaac aacaacgcg 
gctaggaat aacatagat aactgggtc ctggtgccc 
agagatggt actaacacg gactga
    mRNA sequence
    • >TC005572 RA
      aaggataca taaccaaat ttatggtgg ttgatattt 
atcgagatt tttcgtaat tttggggct gtctggacg 
ctctgtggc caaatatgt gattacttc ggtatttcg 
atggcgtct tcggaaaat gagggtagc gtatgtcag 
tacaccgtc gaggaaatc aactacaat gaaattcag 
aagcttgag gcagtcggt gagggcagc tttggggtc 
gtttacaag ggaatctgg cgcgatgcg ttcgtggcg 
gtcaaaaac atcgtctct gagaacgac aaagacgcc 
tttattgtg gaagtccgg cagctgtca agggtcaag 
catgagaac attgtcaag ctgtacggg gcctgcacc 
aagggcccc aacatctgc cttgtgatg gagtacgcc 
gaagggggc tccctcttc aatattcta caccacgca 
cctaacgta cactacacg atggccaac gccatgtcc 
tggctctac cagtgcgcc aaaggggtc gagtatctc 
catggcatg aaacccaaa cctttgata cacagagac 
ctcaaaccg cccaacttg ctcctgata aacgggggc 
accttgcta aagatatgc gactttggt actgccaca 
gacatgaca gcaatcatg accaacaac aaaggctcg 
gccgcgtgg atggccccc gaggtcttt gaaacgtca 
aactatacg gaaaagtgc gacgtcttc agctggggc 
ataatcctc tgggaggtg ttatcgcga cttaagcct 
tttttccac attaaggag tcgggctac ccttcggct 
ttcatgatc ttgtgggcg aagcacagc ggccggagg 
ccccctctc atcaggcac tgcccccca gtcatcgaa 
aaactgatg acacagtgc tgggatcag gaggcggcc 
aagcggccg agcatgaag gagattgtg aaaaaaatg 
gaagttatt tgctcgttg cttccgggc gccgacgag 
ccgttgcag atcaggggc ggtgacgat gtggtttac 
aaggaggaa atcgaagag tttgaggac tgtgtggag 
gaaatagac cagttcccc gccagcacc aacggcgat 
aattgtgtc aaatcgctg ccccagccg agtgccgag 
atgtcgcgg cccctcaac ataaaaata agccctgac 
gcatgggat ttacacgat ctcgacatg cagctgaga 
accatgccc ggccttgat aagatggta ccaaaaaac 
ggttccaaa aacacaacg gtagaggca ggcatcgag 
agcaccaag tcgacacct gacggcacc gatccaaat 
ttagataaa gtgtatctt ttattagag ccgcattta 
cgacctgcc actccagac gccgacaat ccgcagtcg 
ttggcgctg ttcgaagag cacaagcaa ctggctcag 
gaatatttc aaggtccaa actgagctc gcatatgtc 
tcgcaaaag aaggacaaa ctcctagcg gcgcagtcg 
caagaacag cagcagcac accaaactc ctcaaagac 
ctgcaagcg gagaaggag tccctgatc ttggtgcgg 
gagttgctc agtcggcaa agagaaaac aacaacgcg 
gctaggaat aacatagat aactgggtc ctggtgccc 
agagatggt actaacacg gactgactt atttattta 
cctcgacgc tcatttcct tcacccttc atcggttat 
ttatcagag tgtttttct aggataagc tcaattttt 
atatgtgca tatgtaaat aaagctggt tatttata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY