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Gene information
General information
TC005565 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11246087..11249428
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005565
    iBeetle screen iB_00886

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax dorsal segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005565 RA
      MSLLKPSIL LLCVLPLVI SKGLHEEFS WTRINYVWP 
      GQDSSSSSP IDIPKPDPI AFPGSTNNN QRHTTRRPG 
      RTTRRTTQR PDTNPDYVY ENNIPMGAN LWRDKLFVT 
      VPRRRVGVP STVNFVWAN SSQRHNVPL IPYPDWTTN 
      TLRDSRDSR DDGYRFVSV YRVAVDSCD RLWFVDTGL 
      IETPGNPQQ IQPTALVLM DLKTDKILQ YYQFPANLL 
      RNTSNLASL TIDITNNNC RDAFAYIPD VGGYGLVVY 
      SLRQNKAWR VNHNYFYLE NTVGEFSIG GHEFQWNDG 
      IFSIAITDV KSDGFRDAY FHSMAGVNL YKVSTRILK 
      NEDLATRSY HGSDFKIVG NKGELSQTS SSDLHQGKG 
      VLFLGLVNQ NALGCWNIH KPLSDISVV QRSDEKMIY 
      PSDVKIVDD KIVVLTNTM PVFLYGRLD YDRTNFRVW 
      VETVETAIR GTKCESSRG GSHY
    CDS sequence
    • >TC005565 RA
      atgtcactg ctcaagccc tcgatcctc ctcctttgt 
      gtccttcct ctagtaata agcaaagga ctccacgag 
      gagttctcc tggactcgg atcaactac gtctggcct 
      ggacaggac tcctcctcc tcctcccct atcgacatc 
      cctaaaccc gaccccatc gcattccct ggaagcacc 
      aacaacaac caaaggcac accaccagg agacctgga 
      cgcaccact cgccgaaca acccaaaga cctgacacc 
      aatcctgac tatgtttac gaaaacaat atcccgatg 
      ggtgctaat ctttggaga gataagttg tttgtgacg 
      gtgcctcgg aggcgggtt ggggtgcca tcaactgtc 
      aacttcgtc tgggcgaac agtagccaa aggcacaat 
      gtaccattg atcccttat cccgactgg accaccaat 
      actttgcgg gattcgcgg gattcccgg gatgatggt 
      tatcggttc gtttcggtt tatcgggtg gctgtggat 
      tcgtgcgac cgcttatgg tttgtcgat actggactt 
      attgaaact ccaggcaac ccgcaacaa atccaacca 
      actgccttg gtcctcatg gatttgaaa accgacaaa 
      atcctccag tactaccag ttcccggca aacctcctc 
      cggaacacg tcaaacctg gccagtttg accattgat 
      atcactaac aataactgt cgggacgct tttgcctac 
      atcccagat gttgggggt tacggttta gttgtttac 
      agtttgagg caaaataag gcttggagg gtgaaccat 
      aattatttc tatctcgag aacactgtt ggggaattt 
      tcgatcgga ggacatgag tttcaatgg aatgatggg 
      attttcagt attgccatc accgatgtt aaatctgat 
      gggttcaga gatgcttat tttcattct atggctggg 
      gttaatttg tataaagtt tcgaccagg attttgaaa 
      aatgaggat ttggccacg aggtcgtac cacggcagc 
      gattttaag attgttgga aacaaaggt gagctttct 
      caaacttcc tcgtctgat ttgcaccaa ggcaaagga 
      gtgttgttt ctgggtttg gtgaatcag aacgccttg 
      ggttgctgg aacatccac aagcccctg agtgatatt 
      tccgtggtg cagagaagt gacgagaag atgatctac 
      ccaagtgat gtgaaaata gtggacgat aagatcgtc 
      gtattgacc aacaccatg ccggtgttt ctctacgga 
      aggcttgat tacgacagg acgaacttc cgagtgtgg 
      gtggagacg gtggagacg gccatccga ggcacaaaa 
      tgcgagtct tcaagaggc ggatcgcac tattaa
    mRNA sequence
    • >TC005565 RA
      tacttgcct gtattttcc ttcgagcta tatgacaaa 
      taggcgcac gccactgtc ttccctcca ccacaacac 
      gcatcttcg tttagttct taaaaatcg tccgaatgg 
      ttcgcatca tgtcactgc tcaagccct cgatcctcc 
      tcctttgtg tccttcctc tagtaataa gcaaaggac 
      tccacgagg agttctcct ggactcgga tcaactacg 
      tctggcctg gacaggact cctcctcct cctccccta 
      tcgacatcc ctaaacccg accccatcg cattccctg 
      gaagcacca acaacaacc aaaggcaca ccaccagga 
      gacctggac gcaccactc gccgaacaa cccaaagac 
      ctgacacca atcctgact atgtttacg aaaacaata 
      tcccgatgg gtgctaatc tttggagag ataagttgt 
      ttgtgacgg tgcctcgga ggcgggttg gggtgccat 
      caactgtca acttcgtct gggcgaaca gtagccaaa 
      ggcacaatg taccattga tcccttatc ccgactgga 
      ccaccaata ctttgcggg attcgcggg attcccggg 
      atgatggtt atcggttcg tttcggttt atcgggtgg 
      ctgtggatt cgtgcgacc gcttatggt ttgtcgata 
      ctggactta ttgaaactc caggcaacc cgcaacaaa 
      tccaaccaa ctgccttgg tcctcatgg atttgaaaa 
      ccgacaaaa tcctccagt actaccagt tcccggcaa 
      acctcctcc ggaacacgt caaacctgg ccagtttga 
      ccattgata tcactaaca ataactgtc gggacgctt 
      ttgcctaca tcccagatg ttgggggtt acggtttag 
      ttgtttaca gtttgaggc aaaataagg cttggaggg 
      tgaaccata attatttct atctcgaga acactgttg 
      gggaatttt cgatcggag gacatgagt ttcaatgga 
      atgatggga ttttcagta ttgccatca ccgatgtta 
      aatctgatg ggttcagag atgcttatt ttcattcta 
      tggctgggg ttaatttgt ataaagttt cgaccagga 
      ttttgaaaa atgaggatt tggccacga ggtcgtacc 
      acggcagcg attttaaga ttgttggaa acaaaggtg 
      agctttctc aaacttcct cgtctgatt tgcaccaag 
      gcaaaggag tgttgtttc tgggtttgg tgaatcaga 
      acgccttgg gttgctgga acatccaca agcccctga 
      gtgatattt ccgtggtgc agagaagtg acgagaaga 
      tgatctacc caagtgatg tgaaaatag tggacgata 
      agatcgtcg tattgacca acaccatgc cggtgtttc 
      tctacggaa ggcttgatt acgacagga cgaacttcc 
      gagtgtggg tggagacgg tggagacgg ccatccgag 
      gcacaaaat gcgagtctt caagaggcg gatcgcact 
      attaatgca tgctgtttt ttactgtag ttccagtct 
      attgtatca ttgtctttg tgataataa agcttcttt 
      tgtccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY