Status
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Gene information
General information
TC031285
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005556,TC005555,TC005554
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11390581..11397917
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031285
    iBeetle screen iB_00884

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: Sometimes with some identifyable mouthparts
    head capsule partially split - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    metathorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen anterior not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031285 RA
      MENESVLSC SVNIEWSNP QGIIQRKLA HKSAFLRLI 
      RNDAREIFI EVTAGKSVP VKLQLKGIS VHNKFMSEG 
      KASIKFQEE HCMMFLSNA PPNQLFTFL KTVFVKMTG 
      GKPAPTSHQ SLRTQLLSN KPATFEEIS PVTTAEMDK 
      AKAKISKGT DTTPSPLSR KRKLSPRPG AKPASSKRL 
      YAASPTPNE PLDIEQKEV LEACLSGRN VFFTGSAGT 
      GKSFLLKRI IGALPPDVT AATASTGVA ACHIGGITL 
      HQFAGIGAG EAPLERSVE LASRPGAYT VWRKCKHLI 
      IDEISMVDG SYFEKIEAI ARKVRRNEQ PFGGIQLIL 
      CGDFFQLPP VAKGKTPVK FCFETEAWS RCRLSAYEL 
      KKVHRQNDD EFIKILNCV RIGQIPEDM ATRLAETSR 
      QNIEKNGIL ATRLCSHTL EANAINEAK LNALPGEKL 
      VFTSEDSHP HLIKQLDQQ TSIPHTLEL KVGAQVMLL 
      KNINIATGL VNGARGVVK SFQEGVPVV QFRSKEYVA 
      KPERWCVKS ATGVMLTRK QVPLKLAWA FSIHKSQGL 
      TLDCVEMSL GKVFEAGQA YVALSRAQS LDTLRVLGF 
      KASQVWANK NVLDFYRSL NLQMEAMRL IPLGRREDK 
      AAVKKSYKP NAKLMSKPL ASQMRSSVS YILSSSTSE 
      PNMSHPDYE QYSHDHAAL LVLVRHIGS QLKPKTFQK 
      FHDRIAKLT SVRITDSTG NVRTILVRY VKEHPVENN 
      DWGDFQTHR RLLGLVSLG KYDSQQELN EICRVHESL 
      KVKYTGTLF DSRCILFGP TKEASSSPE DTSSPNTDS 
      SSSAPEENI EKFTTPSNF KTRGLFYDE SAPCADLET 
      QIIEFINSL FWVLESKRL ERSREKLER VSLLLAPFE 
      KKDFVGLDM ESRNNKKKC TGRMTKHLG DLCLQAGLL 
      AESLVYYGN AAETLKSVN DWLWLGAAY EGLCAASAL 
      VLYPEMQRS VQLQRNASL QEGSSPKKT VVASNLDVG 
      AKKASVPNT LAPEDISKR YREAIIHYS KYQNAGIVE 
      TEASFKAAR IAVEQNHSL QAASFLQNV VYINLTLSE 
      QEKIQRFET LAELYTQIG FTRKAAFCQ RLAATRYVS 
      PQNPAPNWN RCYNLMLHS LPGHRLSLD PAEMREVEQ 
      GWPILQIQL LQELVVAAK RMGHSALAT RHMTFLLQT 
      MWQHLTPTE QKELAIQLQ ALSAQCEGS PVPLVLDSG 
      VVIPPANLT NIPLCHSFV LRNLQPHLQ PRKIQAVKQ 
      DTGPFLFTP IHFGSLDRR STASQTKMA FLWVENEPC 
      EVTLKLSNP LPFELKVSD MRLLTSGVV FESLPETVV 
      LSPSGAPPS VTTLHGTPR ESEGQLQLQ GYSTHTLGV 
      KSNCRLRHM NGFPPHYEV EIVPSLPLL EVKTSLPQS 
      ASFSSFHDY ENVVTSASV SLHHGESAE CTVTITNIG 
      HVDVEMLEV SMQTVLEPA LQEQMFTWS QENLLSQLP 
      LKPNCSASL TLYLYAAGS FLVPGGTGN EFSSGVFSS 
      SISGPGSLP SRLNSPTSS SFHRNVNSS FRSSNSGHS 
      SMSATLQIP HLPINQQAS SVVEGQLQI RYSGGPGLQ 
      ASYCRCCSV FLTIETVPS LHVTNWDVL PAETNTQFY 
      LVLDVANLT SQEMELQYT PSKTMLIEG HESCRVPVP 
      VKRCPLSKL ETGETLATG ELDRICSEH IANLVDLRW 
      HLLGTDTRG KASLKGLTL TNDMLDEVR MSPLLWEVT 
      INNETVRPQ EEVTCDAGD CLEMKIFVT NSLDKVLSQ 
      LQLSVQFYQ DYENGTLNY RMETRLATT GTTKKMIKS 
      LEPQEKAQH ECNVIFFTP GQYKIDIQC SAPDNSTAV 
      PCLPPSGHT WRFTPPVNV LYNNHQKHL RIQEIHERM 
      TEIAETMHS VEAVANTTS VLVSKLEKT VKRRTLRGR 
      LKKIKTR
    CDS sequence
    • >TC031285 RA
      atggaaaac gagagtgtg ttatcatgt tcggtgaat 
      atcgagtgg tcgaacccc caaggcatc atccaacga 
      aaactggcc cacaagagc gccttcctg cgcttaatt 
      cgcaacgat gcccgtgaa atcttcatt gaagtgacc 
      gcaggcaag tccgtcccc gtcaagctc caactgaaa 
      ggcatttcc gtccacaac aagttcatg agcgagggc 
      aaagcctcc ataaaattc caagaagag cactgcatg 
      atgttccta tcaaacgcg cccccgaac cagcttttc 
      accttcctg aagaccgtg tttgtgaaa atgacgggc 
      gggaaaccg gccccgacc tcccaccag tccttgagg 
      actcaatta ctgtcgaat aaaccggcc acgtttgag 
      gaaatcagt cccgtgact acggccgaa atggacaag 
      gctaaggcc aagatttcg aaagggacg gacacgacg 
      ccgtcgcct ttgagccgc aaacgcaag ctgagcccc 
      cggccgggg gccaaaccc gcctcctcc aagaggctg 
      tacgcggct tcgcccacc cccaacgag cccctggat 
      atcgagcag aaggaggtg ctggaggct tgcctcagc 
      ggacgcaac gtgttcttc acggggagt gcaggcacg 
      gggaaaagc tttcttttg aagaggatt attggggcg 
      ctgccgccc gacgtgacc gcagccact gcctccaca 
      ggtgtcgcc gcttgccac atcgggggg atcaccctg 
      caccagttc gccgggatc ggggccggc gaggcgccc 
      ctggagcgc tccgtcgag ctggcgtcg cgcccgggg 
      gcttacacg gtctggcgc aagtgcaag cacctgatc 
      atcgacgag atttcgatg gttgacggg tcgtatttc 
      gagaaaatc gaggcaata gcccggaaa gtcaggcgg 
      aatgagcag cctttcggc gggattcag ttgattttg 
      tgcggggat tttttccaa ttgcctccg gtggccaaa 
      ggcaaaact ccagtcaag ttctgtttc gagacggag 
      gcctggagt cggtgccga ttgtcggcg tatgaactg 
      aaaaaagtc caccggcaa aacgacgac gagtttatc 
      aaaatcttg aattgtgtg cgcattgga caaattcct 
      gaagatatg gcgacgaga ttggccgaa acgtcgcga 
      cagaatatc gaaaagaac gggattttg gccacgagg 
      ttgtgctcg cacacgctg gaagcgaac gcgattaac 
      gaagcgaaa cttaacgca ctgccgggc gaaaaactc 
      gtctttacg tccgaagac tcacacccg catcttatc 
      aagcagttg gatcagcaa acctccatc ccgcacacg 
      ctcgaattg aaggtcggt gcgcaggtg atgcttttg 
      aaaaacata aacatcgcc acggggctc gtgaacggc 
      gctcgtgga gtcgtcaaa tccttccaa gaaggtgtt 
      cccgttgtg caattccgt agcaaagaa tatgtcgca 
      aagcctgag cgctggtgc gtcaaaagc gccaccggt 
      gttatgctc acgaggaaa caggtgccc cttaaacta 
      gcgtgggct ttttccatt cacaagtcc caaggtttg 
      acgttagat tgtgttgaa atgtcgcta ggaaaggtg 
      tttgaagcc ggtcaagct tatgttgcg ttaagtagg 
      gcccaaagt ttagacacg ctgagggtt ttagggttt 
      aaggcctcg caagtctgg gccaataaa aatgttttg 
      gatttttat cggtcgttg aacttgcag atggaggcc 
      atgagactt attcccttg gggaggaga gaggacaag 
      gcagcggtt aaaaaatcc tacaaacct aatgcgaaa 
      ttgatgagc aaaccttta gcctcacaa atgcgttct 
      tcggtaagt tacatcctt tcgagtagt accagcgag 
      ccaaacatg tctcacccc gactacgaa caatactcc 
      catgaccac gctgctctc ctggtgctc gtacgacac 
      ataggctcc caactaaag cccaaaaca ttccaaaaa 
      ttccacgac cgtatcgcc aaactcacc tcagtccgc 
      atcaccgac tcgacggga aacgtccgc acgattcta 
      gtccgttat gtgaaggaa caccccgtt gaaaacaac 
      gattggggc gacttccaa acccaccga cgactctta 
      ggtctcgtc tctctggga aaatacgac tcccagcaa 
      gagctcaat gaaatatgc cgcgtccac gagagtctc 
      aaagtcaaa tacaccggc acgttattc gattcgcgc 
      tgcattttg ttcggcccc acgaaggag gcgagcagc 
      tcgccggaa gacacctcg agccccaac accgacagc 
      agcagcagc gcccccgag gagaacatc gagaagttc 
      acaacgcct agcaacttc aagacacgg gggctcttc 
      tacgacgag agcgccccg tgtgctgac ctcgaaacc 
      caaataatc gaattcatc aattcgctg ttttgggtg 
      cttgagtcc aaacgactc gaacgctcg cgcgaaaag 
      ctcgaacgg gtctccctc ctgctcgcc ccctttgag 
      aaaaaggac ttcgtgggg ctcgacatg gagtcgcgc 
      aacaacaag aaaaaatgc acaggccgg atgaccaaa 
      cacttgggc gatttgtgt ctgcaagcg gggctgctg 
      gccgaaagc ctggtttat tacggcaat gcagccgag 
      acgttgaag agtgtcaac gattggttg tggctgggg 
      gccgcctat gagggtttg tgtgccgct tctgctcta 
      gttttgtac cccgagatg cagcgaagt gtgcaattg 
      caacgtaac gccagtttg caagagggt tcctcgccg 
      aaaaaaaca gtagtcgcg agtaatctt gacgtgggg 
      gcgaagaaa gcctccgtg cccaacaca ctagccccc 
      gaggatatc tcgaagagg tatcgggag gcgattatt 
      cactacagc aagtatcaa aatgcgggg atagtcgag 
      actgaggcg agttttaag gccgcccgc atcgctgtt 
      gagcaaaac cactcactt caagccgcc tcgtttcta 
      caaaacgtg gtctacatc aatttaacg ctctcagag 
      caggaaaaa atccagcgt tttgagacc ttggccgaa 
      ctttatacg caaattggt ttcacgcgt aaagccgcc 
      ttctgccaa cggctcgcc gccaccaga tacgtctcg 
      ccccaaaat cccgccccc aattggaac cgctgctat 
      aacctcatg ctccacagc cttcccggc catcgccta 
      tctttagat ccggccgaa atgagggag gttgaacaa 
      gggtggcca attctacaa attcaattg ttacaagaa 
      ctggtggtg gcggcgaaa agaatgggg cattcggcg 
      ttggcgact cgccacatg acgtttttg ttgcaaaca 
      atgtggcag catttgacg ccgactgag cagaaagaa 
      ctggcgata cagttacaa gccttatcg gcgcaatgt 
      gaagggtca ccggtgccc ttggtgttg gacagtggc 
      gttgttata ccaccggcc aacttgacc aatattcca 
      ctttgccat tcttttgtt ttgaggaac ttgcagccc 
      catttacag ccgcggaaa atacaggct gtaaagcag 
      gacacgggg ccgtttttg ttcactcca attcatttt 
      gggagtttg gatcggagg agcacagcg agtcaaact 
      aaaatggca tttttgtgg gtggagaac gagccttgc 
      gaagtcacc ctcaagctg tccaacccc ctgcccttc 
      gagttgaag gtttcggac atgcgtttg ttgacatca 
      ggtgtcgtg tttgagtca ctccccgaa actgtggtt 
      ttatcaccg tcgggggct cccccatct gttacgacc 
      ctccatggc acgccgcgc gaaagcgaa ggccagctc 
      caactccaa ggctacagc acgcacacc cttggcgtc 
      aaatccaac tgtcggttg agacacatg aacgggttt 
      ccaccccat tacgaggtc gaaatcgtc ccatccctt 
      cccttactc gaggtgaaa acatcgctc cctcaaagc 
      gcctccttc tcctcattc cacgactac gaaaacgtc 
      gtaacctca gccagtgtg agtctccac cacggcgaa 
      agcgccgaa tgcacagtc accatcaca aatatcggg 
      catgttgac gtggagatg ctcgaagtt tcgatgcag 
      acggtcctg gaaccggcc ctccaagaa cagatgttc 
      acttggtcg caggagaac ctcctaagt caattgccc 
      ctaaaaccg aattgttcg gctagtctg acgttgtat 
      ttgtacgcc gctgggagt tttctcgtc ccgggaggc 
      acaggaaat gaattcagc agtggcgtt ttcagtagc 
      agtatttca ggcccgggc tcgttaccc tcgcgactg 
      aattcacca actagtagc agttttcac agaaatgtt 
      aattcgagc tttcggtcc tcgaattcg ggccattcg 
      agcatgagc gccacttta caaatcccc cacttgccc 
      ataaaccag caagcatcg tccgttgtt gagggacag 
      ttgcagata cggtacagt ggggggcca ggcttgcaa 
      gcgtcgtat tgtcggtgt tgttcggtt tttttgacg 
      attgagacg gtgccctcg ctgcatgtt accaactgg 
      gatgtatta ccggctgag acgaacact cagttttat 
      ttagtcttg gatgtggcc aacttgacg tcgcaagaa 
      atggaatta cagtacacc ccgtccaag acaatgctc 
      atagaaggg cacgaaagt tgcagagtg cctgtgccg 
      gtaaagcgg tgcccttta tcgaagctc gaaaccggg 
      gaaaccctc gcaactggc gaattggat cggatttgt 
      tcggaacac atcgccaat cttgtcgac ttgaggtgg 
      cacctgctt ggcaccgac accagaggc aaagcctcc 
      ctcaaaggt ctcaccttg accaatgat atgcttgat 
      gaagtgaga atgtcgccg ttattatgg gaagtgacg 
      ataaataat gaaacagtc aggccgcag gaggaagtc 
      acgtgcgac gctggtgat tgtttagaa atgaaaata 
      ttcgtgacg aatagtttg gataaagtt ctgagtcaa 
      ttacaattg tcagtccag ttttaccaa gattatgaa 
      aatggtact ctaaattat aggatggaa acgaggtta 
      gcgacaacc gggactaca aagaaaatg atcaagtcg 
      ttggagccc caagaaaag gcgcaacat gagtgcaat 
      gttattttc ttcactcct ggacagtat aaaatcgat 
      attcagtgc tcagcacct gataattcg accgcagtt 
      ccgtgttta ccgccaagt gggcacacc tggaggttc 
      acaccgcct gttaatgta ttgtataac aaccaccag 
      aagcattta cggatacaa gagatccac gagagaatg 
      actgaaatc gcagaaact atgcacagt gtcgaagcg 
      gtcgcgaac accacttcg gttctagtt agcaagttg 
      gaaaagact gtgaaacgg aggacgctt aggggcaga 
      ttaaaaaaa attaaaacg aggtga
    mRNA sequence
    • >TC031285 RA
      aaagtttga actttgggg gttcgttag aggttattt 
      acttttttc ccttatttt aatccgcgt gaatgaaac 
      tcgcattat ctaacaatg gaaaacgag agtgtgtta 
      tcatgttcg gtgaatatc gagtggtcg aacccccaa 
      ggcatcatc caacgaaaa ctggcccac aagagcgcc 
      ttcctgcgc ttaattcgc aacgatgcc cgtgaaatc 
      ttcattgaa gtgaccgca ggcaagtcc gtccccgtc 
      aagctccaa ctgaaaggc atttccgtc cacaacaag 
      ttcatgagc gagggcaaa gcctccata aaattccaa 
      gaagagcac tgcatgatg ttcctatca aacgcgccc 
      ccgaaccag cttttcacc ttcctgaag accgtgttt 
      gtgaaaatg acgggcggg aaaccggcc ccgacctcc 
      caccagtcc ttgaggact caattactg tcgaataaa 
      ccggccacg tttgaggaa atcagtccc gtgactacg 
      gccgaaatg gacaaggct aaggccaag atttcgaaa 
      gggacggac acgacgccg tcgcctttg agccgcaaa 
      cgcaagctg agcccccgg ccgggggcc aaacccgcc 
      tcctccaag aggctgtac gcggcttcg cccaccccc 
      aacgagccc ctggatatc gagcagaag gaggtgctg 
      gaggcttgc ctcagcgga cgcaacgtg ttcttcacg 
      gggagtgca ggcacgggg aaaagcttt cttttgaag 
      aggattatt ggggcgctg ccgcccgac gtgaccgca 
      gccactgcc tccacaggt gtcgccgct tgccacatc 
      ggggggatc accctgcac cagttcgcc gggatcggg 
      gccggcgag gcgcccctg gagcgctcc gtcgagctg 
      gcgtcgcgc ccgggggct tacacggtc tggcgcaag 
      tgcaagcac ctgatcatc gacgagatt tcgatggtt 
      gacgggtcg tatttcgag aaaatcgag gcaatagcc 
      cggaaagtc aggcggaat gagcagcct ttcggcggg 
      attcagttg attttgtgc ggggatttt ttccaattg 
      cctccggtg gccaaaggc aaaactcca gtcaagttc 
      tgtttcgag acggaggcc tggagtcgg tgccgattg 
      tcggcgtat gaactgaaa aaagtccac cggcaaaac 
      gacgacgag tttatcaaa atcttgaat tgtgtgcgc 
      attggacaa attcctgaa gatatggcg acgagattg 
      gccgaaacg tcgcgacag aatatcgaa aagaacggg 
      attttggcc acgaggttg tgctcgcac acgctggaa 
      gcgaacgcg attaacgaa gcgaaactt aacgcactg 
      ccgggcgaa aaactcgtc tttacgtcc gaagactca 
      cacccgcat cttatcaag cagttggat cagcaaacc 
      tccatcccg cacacgctc gaattgaag gtcggtgcg 
      caggtgatg cttttgaaa aacataaac atcgccacg 
      gggctcgtg aacggcgct cgtggagtc gtcaaatcc 
      ttccaagaa ggtgttccc gttgtgcaa ttccgtagc 
      aaagaatat gtcgcaaag cctgagcgc tggtgcgtc 
      aaaagcgcc accggtgtt atgctcacg aggaaacag 
      gtgcccctt aaactagcg tgggctttt tccattcac 
      aagtcccaa ggtttgacg ttagattgt gttgaaatg 
      tcgctagga aaggtgttt gaagccggt caagcttat 
      gttgcgtta agtagggcc caaagttta gacacgctg 
      agggtttta gggtttaag gcctcgcaa gtctgggcc 
      aataaaaat gttttggat ttttatcgg tcgttgaac 
      ttgcagatg gaggccatg agacttatt cccttgggg 
      aggagagag gacaaggca gcggttaaa aaatcctac 
      aaacctaat gcgaaattg atgagcaaa cctttagcc 
      tcacaaatg cgttcttcg gtaagttac atcctttcg 
      agtagtacc agcgagcca aacatgtct caccccgac 
      tacgaacaa tactcccat gaccacgct gctctcctg 
      gtgctcgta cgacacata ggctcccaa ctaaagccc 
      aaaacattc caaaaattc cacgaccgt atcgccaaa 
      ctcacctca gtccgcatc accgactcg acgggaaac 
      gtccgcacg attctagtc cgttatgtg aaggaacac 
      cccgttgaa aacaacgat tggggcgac ttccaaacc 
      caccgacga ctcttaggt ctcgtctct ctgggaaaa 
      tacgactcc cagcaagag ctcaatgaa atatgccgc 
      gtccacgag agtctcaaa gtcaaatac accggcacg 
      ttattcgat tcgcgctgc attttgttc ggccccacg 
      aaggaggcg agcagctcg ccggaagac acctcgagc 
      cccaacacc gacagcagc agcagcgcc cccgaggag 
      aacatcgag aagttcaca acgcctagc aacttcaag 
      acacggggg ctcttctac gacgagagc gccccgtgt 
      gctgacctc gaaacccaa ataatcgaa ttcatcaat 
      tcgctgttt tgggtgctt gagtccaaa cgactcgaa 
      cgctcgcgc gaaaagctc gaacgggtc tccctcctg 
      ctcgccccc tttgagaaa aaggacttc gtggggctc 
      gacatggag tcgcgcaac aacaagaaa aaatgcaca 
      ggccggatg accaaacac ttgggcgat ttgtgtctg 
      caagcgggg ctgctggcc gaaagcctg gtttattac 
      ggcaatgca gccgagacg ttgaagagt gtcaacgat 
      tggttgtgg ctgggggcc gcctatgag ggtttgtgt 
      gccgcttct gctctagtt ttgtacccc gagatgcag 
      cgaagtgtg caattgcaa cgtaacgcc agtttgcaa 
      gagggttcc tcgccgaaa aaaacagta gtcgcgagt 
      aatcttgac gtgggggcg aagaaagcc tccgtgccc 
      aacacacta gcccccgag gatatctcg aagaggtat 
      cgggaggcg attattcac tacagcaag tatcaaaat 
      gcggggata gtcgagact gaggcgagt tttaaggcc 
      gcccgcatc gctgttgag caaaaccac tcacttcaa 
      gccgcctcg tttctacaa aacgtggtc tacatcaat 
      ttaacgctc tcagagcag gaaaaaatc cagcgtttt 
      gagaccttg gccgaactt tatacgcaa attggtttc 
      acgcgtaaa gccgccttc tgccaacgg ctcgccgcc 
      accagatac gtctcgccc caaaatccc gcccccaat 
      tggaaccgc tgctataac ctcatgctc cacagcctt 
      cccggccat cgcctatct ttagatccg gccgaaatg 
      agggaggtt gaacaaggg tggccaatt ctacaaatt 
      caattgtta caagaactg gtggtggcg gcgaaaaga 
      atggggcat tcggcgttg gcgactcgc cacatgacg 
      tttttgttg caaacaatg tggcagcat ttgacgccg 
      actgagcag aaagaactg gcgatacag ttacaagcc 
      ttatcggcg caatgtgaa gggtcaccg gtgcccttg 
      gtgttggac agtggcgtt gttatacca ccggccaac 
      ttgaccaat attccactt tgccattct tttgttttg 
      aggaacttg cagccccat ttacagccg cggaaaata 
      caggctgta aagcaggac acggggccg tttttgttc 
      actccaatt cattttggg agtttggat cggaggagc 
      acagcgagt caaactaaa atggcattt ttgtgggtg 
      gagaacgag ccttgcgaa gtcaccctc aagctgtcc 
      aaccccctg cccttcgag ttgaaggtt tcggacatg 
      cgtttgttg acatcaggt gtcgtgttt gagtcactc 
      cccgaaact gtggtttta tcaccgtcg ggggctccc 
      ccatctgtt acgaccctc catggcacg ccgcgcgaa 
      agcgaaggc cagctccaa ctccaaggc tacagcacg 
      cacaccctt ggcgtcaaa tccaactgt cggttgaga 
      cacatgaac gggtttcca ccccattac gaggtcgaa 
      atcgtccca tcccttccc ttactcgag gtgaaaaca 
      tcgctccct caaagcgcc tccttctcc tcattccac 
      gactacgaa aacgtcgta acctcagcc agtgtgagt 
      ctccaccac ggcgaaagc gccgaatgc acagtcacc 
      atcacaaat atcgggcat gttgacgtg gagatgctc 
      gaagtttcg atgcagacg gtcctggaa ccggccctc 
      caagaacag atgttcact tggtcgcag gagaacctc 
      ctaagtcaa ttgccccta aaaccgaat tgttcggct 
      agtctgacg ttgtatttg tacgccgct gggagtttt 
      ctcgtcccg ggaggcaca ggaaatgaa ttcagcagt 
      ggcgttttc agtagcagt atttcaggc ccgggctcg 
      ttaccctcg cgactgaat tcaccaact agtagcagt 
      tttcacaga aatgttaat tcgagcttt cggtcctcg 
      aattcgggc cattcgagc atgagcgcc actttacaa 
      atcccccac ttgcccata aaccagcaa gcatcgtcc 
      gttgttgag ggacagttg cagatacgg tacagtggg 
      gggccaggc ttgcaagcg tcgtattgt cggtgttgt 
      tcggttttt ttgacgatt gagacggtg ccctcgctg 
      catgttacc aactgggat gtattaccg gctgagacg 
      aacactcag ttttattta gtcttggat gtggccaac 
      ttgacgtcg caagaaatg gaattacag tacaccccg 
      tccaagaca atgctcata gaagggcac gaaagttgc 
      agagtgcct gtgccggta aagcggtgc cctttatcg 
      aagctcgaa accggggaa accctcgca actggcgaa 
      ttggatcgg atttgttcg gaacacatc gccaatctt 
      gtcgacttg aggtggcac ctgcttggc accgacacc 
      agaggcaaa gcctccctc aaaggtctc accttgacc 
      aatgatatg cttgatgaa gtgagaatg tcgccgtta 
      ttatgggaa gtgacgata aataatgaa acagtcagg 
      ccgcaggag gaagtcacg tgcgacgct ggtgattgt 
      ttagaaatg aaaatattc gtgacgaat agtttggat 
      aaagttctg agtcaatta caattgtca gtccagttt 
      taccaagat tatgaaaat ggtactcta aattatagg 
      atggaaacg aggttagcg acaaccggg actacaaag 
      aaaatgatc aagtcgttg gagccccaa gaaaaggcg 
      caacatgag tgcaatgtt attttcttc actcctgga 
      cagtataaa atcgatatt cagtgctca gcacctgat 
      aattcgacc gcagttccg tgtttaccg ccaagtggg 
      cacacctgg aggttcaca ccgcctgtt aatgtattg 
      tataacaac caccagaag catttacgg atacaagag 
      atccacgag agaatgact gaaatcgca gaaactatg 
      cacagtgtc gaagcggtc gcgaacacc acttcggtt 
      ctagttagc aagttggaa aagactgtg aaacggagg 
      acgcttagg ggcagatta aaaaaaatt aaaacgagg 
      tgatggtaa tttccgtaa aatattcta acgcaagcg 
      cttggtcaa ttcacactt atcgctttt gtttttaat 
      ttgtgttat cactggaga agtctagcc gttgttgac 
      taatttatt aaaatgttg cgtgtgat
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