Status
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Gene information
General information
TC005545 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11463155..11466747
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005545
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00880

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005545 RA
      MYLLFVLIS CVIERSLCS QDLEVNSSH FNEIVVRHG 
      ERDGSRYDH HFKRYCQKP AKCQLLNYT TCMGMKLPY 
      FSTTLELTD LTTQEKVQE KLHHYKYLR FIPKCWAVI 
      QPFLCALYM PKCENGKVD LPSREMCQL TLKPCKMFY 
      NSSIFPKFL NCDDERIFS SNCKNDVQE LRFNTTGFC 
      MEPLVKTDN QNWFYPDVE ECGLRCKDS LYTENEHYQ 
      IHKLIGYCV LVCGLFNFF TIVTFIIDW TTANKYPAL 
      SIFYMNICF FISYSGWFI QFLSTDTRE DIVCKKDGT 
      LRKSEPSAT ENLSCVIVF VLVYYFLIA GMVWFVLFS 
      YTWHMSCVQ ALGKIQERV DKKRVYFHL IAWSLPLMF 
      TITTMAVGE IDGDYVTGI CFVGIFNFA ARAGLLLAP 
      LAVTMIVAG YIIVRGLVL LIKVKIDSR EIISEHSSR 
      KIRSNIVRM GVFTLFMIV FCIITFIYH GYVFVNSET 
      WNLSLQSYI LCKLTSLSS DFSHCKQES RPSVAMLQL 
      QLLAVFGAG IAMASWVWC SATLHSWTR YIRKKFRCE 
      VEEPMKLQK HKIIAQIFD KREKFNQGG RISIECQNY 
      TDPVGLHFD LNSAVSDSF STAWAVNLP RLLNRRGAI 
      VEEVGYSSN NHSIDSEVS HVSVRHVSI ESRCNSGDS 
      QLSVQISEL KATRKVNRR HRRRRHDFR RDSRLSKTG 
      NKRESSASL DSNLMDTLR LCGVKSLEP NLKRRTGNA 
      GLDGQHISK LLSNGKLVI PYNPNVSED ENVSVTVSE 
      NKYNVVLSN TNLDLSDQL VMKSLRQGG LHIEELSSS 
      ESEKEQEKR PRSGRESHQ SRRSKRSKT SKKYQSDSE 
      SFNRQSDSS SSPELRELL
    CDS sequence
    • >TC005545 RA
      atgtacctc ttgtttgtg ctaatttcg tgtgtgatt 
      gaacgctct ttgtgctcg caagacctc gaagtgaac 
      tcatcacac ttcaacgaa atcgtcgta agacacggt 
      gaaagagac ggctcacgg tacgaccac cacttcaaa 
      aggtactgc caaaagccg gccaaatgc cagcttttg 
      aactacacc acgtgcatg ggcatgaaa ctgccctat 
      ttctccacc acgttggag ctcaccgac ttgacgacg 
      caggaaaaa gtgcaagag aagctccac cattacaaa 
      tatctgagg tttattccc aaatgctgg gcggtcata 
      cagcccttt ctgtgcgct ttgtacatg ccgaagtgc 
      gagaacggt aaagtcgac ctaccgtcg cgggaaatg 
      tgccagctc acactcaaa ccctgcaaa atgttctac 
      aacagctcg attttcccc aaattcctc aattgcgac 
      gacgaacgc attttctcg tcgaattgc aagaacgac 
      gttcaggag ttgaggttc aacaccacg gggttctgc 
      atggagcct ctggtcaag accgacaac cagaattgg 
      ttttatcca gacgtggag gagtgcggc ctccgatgt 
      aaggactcc ctctacacc gagaacgaa cactaccag 
      atccacaaa ctgatcggt tactgcgtc ctggtgtgc 
      ggcctcttc aacttcttc accatcgtg acgttcatc 
      atcgactgg acaacggcc aacaaatac cccgcctta 
      tctattttc tacatgaac atttgcttt tttatttcg 
      tacagcggt tggttcatc caattttta agtacagac 
      acgcgcgaa gacatcgtt tgcaaaaaa gacggaact 
      ctgcgtaaa tccgaaccc agcgccacc gaaaacctg 
      tcctgcgtt atagttttc gtcctcgtt tactatttc 
      ctaatcgca ggaatggtt tggttcgtc cttttcagc 
      tacacgtgg cacatgagc tgcgtccaa gcgctcggg 
      aaaatacaa gaacgcgtt gataaaaag cgcgtttat 
      tttcattta atcgcgtgg agtttgccc ttgatgttc 
      accatcacg accatggcg gtgggcgaa attgacggg 
      gattacgtt acggggatc tgtttcgtt gggattttt 
      aatttcgcg gcaagagct ggactcttg ctggcaccg 
      ttggccgtt acgatgatc gtggcgggg tatattata 
      gtcagaggg ctggttctc ttgatcaaa gtcaaaatt 
      gatagtcgg gaaattatc tcggagcac tcgagtcgc 
      aaaatacgg tcgaatatt gtcaggatg ggcgttttc 
      accctcttt atgatcgtc ttctgcatc atcaccttc 
      atctaccac ggatacgtt ttcgtcaac tcggagacg 
      tggaatctg agtctccag agctacatt ttatgcaaa 
      ttaacgagt ctgagctcg gacttctcc cactgcaag 
      caggagtct cggcccagc gttgcaatg ctccaactg 
      cagctcctt gcagttttc ggcgccgga atcgccatg 
      gcctcctgg gtatggtgc agtgcgacc ctccacagt 
      tggacccgc tacatccgg aagaaattt cgttgcgag 
      gtggaggag ccaatgaaa ctccagaaa cacaaaatc 
      atagcgcag attttcgac aagagggag aagtttaat 
      caagggggg cgcatctca atcgagtgc caaaactac 
      acggacccc gtggggcta catttcgac ctaaacagt 
      gccgttagt gatagtttt agcacggcc tgggctgtg 
      aatctcccc cgtttgtta aacagaaga ggagcaatt 
      gtagaagaa gtgggttat tcaagcaat aatcactcg 
      attgactcg gaagtgtct cacgtctcg gtgagacac 
      gtgagcatc gaaagtcgc tgtaattcg ggcgatagt 
      cagttgtcc gtccaaatc agcgaattg aaagccacc 
      agaaaagtt aaccgacga cacagacgg agacgacac 
      gattttagg agagactcc cgattgtct aaaaccggg 
      aataaacgg gagagcagc gctagcctt gattcgaat 
      ttaatggat acgttacga ttatgcgga gttaaaagt 
      ctagaaccg aacttaaaa cggcggacg ggtaacgcg 
      ggcttggac ggacaacac atcagtaaa ctcctctca 
      aatgggaaa ctcgtaatc ccctataat cccaacgtg 
      agtgaggat gaaaacgtg tcagtgaca gtctcggaa 
      aataaatac aacgtagtt ttgagtaac actaacttg 
      gatttgagt gaccagctc gtgatgaaa tcgttgagg 
      caaggaggc ttgcacatt gaggaattg agtagtagt 
      gagagtgaa aaggagcaa gagaaaagg ccgaggtcg 
      gggcgagaa tcgcaccaa agtcggagg tcaaagagg 
      tccaaaacc tcaaaaaaa tatcaaagc gactcggag 
      agtttcaac agacagagt gacagctcg tcgagtcca 
      gaactgaga gagttgtta tga
    mRNA sequence
    • >TC005545 RA
      atcgttaaa ttaaacgta cgatggccc cgtccctca 
      aaaatcata cagtaaatc aatgtttag gtcaagcac 
      aatatttat aaacaaatt aaagtttta aatgagtgt 
      ttttcaaat gtcgccatt tgggtcgtt tggacttaa 
      gtcataaaa ttccaaata tgcgcctaa ttgccgccc 
      caaacaaag ctaattaga ataatttgc acgtcgtga 
      aagccgcga cacatcctt ggccagtgc agtgttgtc 
      ttgtctttg tcccaactc ctataaaat cctgaaaat 
      actttgacc ttgatgcgt gaggtccat ccacagtcc 
      ccatctccc tcgctctca tccacttgt tcccgtctc 
      ctcttctcg gaaactatg agcctccca tgtgggtgg 
      tcccgctgt ctccttctc ccccaataa aaccgtcta 
      ttcttgtct ctatcgctc ctcccatcc ggcgcaagc 
      caaacacca gcacgtgca gttttcggc ggagtttcc 
      cttcaaaaa tgcgaaaat gtacctctt gtttgtgct 
      aatttcgtg tgtgattga acgctcttt gtgctcgca 
      agacctcga agtgaactc atcacactt caacgaaat 
      cgtcgtaag acacggtga aagagacgg ctcacggta 
      cgaccacca cttcaaaag gtactgcca aaagccggc 
      caaatgcca gcttttgaa ctacaccac gtgcatggg 
      catgaaact gccctattt ctccaccac gttggagct 
      caccgactt gacgacgca ggaaaaagt gcaagagaa 
      gctccacca ttacaaata tctgaggtt tattcccaa 
      atgctgggc ggtcataca gccctttct gtgcgcttt 
      gtacatgcc gaagtgcga gaacggtaa agtcgacct 
      accgtcgcg ggaaatgtg ccagctcac actcaaacc 
      ctgcaaaat gttctacaa cagctcgat tttccccaa 
      attcctcaa ttgcgacga cgaacgcat tttctcgtc 
      gaattgcaa gaacgacgt tcaggagtt gaggttcaa 
      caccacggg gttctgcat ggagcctct ggtcaagac 
      cgacaacca gaattggtt ttatccaga cgtggagga 
      gtgcggcct ccgatgtaa ggactccct ctacaccga 
      gaacgaaca ctaccagat ccacaaact gatcggtta 
      ctgcgtcct ggtgtgcgg cctcttcaa cttcttcac 
      catcgtgac gttcatcat cgactggac aacggccaa 
      caaataccc cgccttatc tattttcta catgaacat 
      ttgcttttt tatttcgta cagcggttg gttcatcca 
      atttttaag tacagacac gcgcgaaga catcgtttg 
      caaaaaaga cggaactct gcgtaaatc cgaacccag 
      cgccaccga aaacctgtc ctgcgttat agttttcgt 
      cctcgttta ctatttcct aatcgcagg aatggtttg 
      gttcgtcct tttcagcta cacgtggca catgagctg 
      cgtccaagc gctcgggaa aatacaaga acgcgttga 
      taaaaagcg cgtttattt tcatttaat cgcgtggag 
      tttgccctt gatgttcac catcacgac catggcggt 
      gggcgaaat tgacgggga ttacgttac ggggatctg 
      tttcgttgg gatttttaa tttcgcggc aagagctgg 
      actcttgct ggcaccgtt ggccgttac gatgatcgt 
      ggcggggta tattatagt cagagggct ggttctctt 
      gatcaaagt caaaattga tagtcggga aattatctc 
      ggagcactc gagtcgcaa aatacggtc gaatattgt 
      caggatggg cgttttcac cctctttat gatcgtctt 
      ctgcatcat caccttcat ctaccacgg atacgtttt 
      cgtcaactc ggagacgtg gaatctgag tctccagag 
      ctacatttt atgcaaatt aacgagtct gagctcgga 
      cttctccca ctgcaagca ggagtctcg gcccagcgt 
      tgcaatgct ccaactgca gctccttgc agttttcgg 
      cgccggaat cgccatggc ctcctgggt atggtgcag 
      tgcgaccct ccacagttg gacccgcta catccggaa 
      gaaatttcg ttgcgaggt ggaggagcc aatgaaact 
      ccagaaaca caaaatcat agcgcagat tttcgacaa 
      gagggagaa gtttaatca aggggggcg catctcaat 
      cgagtgcca aaactacac ggaccccgt ggggctaca 
      tttcgacct aaacagtgc cgttagtga tagttttag 
      cacggcctg ggctgtgaa tctcccccg tttgttaaa 
      cagaagagg agcaattgt agaagaagt gggttattc 
      aagcaataa tcactcgat tgactcgga agtgtctca 
      cgtctcggt gagacacgt gagcatcga aagtcgctg 
      taattcggg cgatagtca gttgtccgt ccaaatcag 
      cgaattgaa agccaccag aaaagttaa ccgacgaca 
      cagacggag acgacacga ttttaggag agactcccg 
      attgtctaa aaccgggaa taaacggga gagcagcgc 
      tagccttga ttcgaattt aatggatac gttacgatt 
      atgcggagt taaaagtct agaaccgaa cttaaaacg 
      gcggacggg taacgcggg cttggacgg acaacacat 
      cagtaaact cctctcaaa tgggaaact cgtaatccc 
      ctataatcc caacgtgag tgaggatga aaacgtgtc 
      agtgacagt ctcggaaaa taaatacaa cgtagtttt 
      gagtaacac taacttgga tttgagtga ccagctcgt 
      gatgaaatc gttgaggca aggaggctt gcacattga 
      ggaattgag tagtagtga gagtgaaaa ggagcaaga 
      gaaaaggcc gaggtcggg gcgagaatc gcaccaaag 
      tcggaggtc aaagaggtc caaaacctc aaaaaaata 
      tcaaagcga ctcggagag tttcaacag acagagtga 
      cagctcgtc gagtccaga actgagaga gttgttatg 
      aggaacgac tgattttat acgaaaagc agcttcgta 
      tttatttgt acaaaaatg tgattttta taactttta 
      ccaaataaa aaattataa gcctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY