| General information | |||
| TC005521 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 11802440..11827163 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005521 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00875 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC005521 RA
MCKLNHCRH KKSLHLRSG GDDCRMPAE GGAPGSDFA HRDRPHQRN INHAGHGGH CYDPEYARM ESWLDEHPD FVQDYFLRK ATRQVVDSW LVSHATPTS SSVELASPT HNQSRAGSG ATTPVRKIS AHEFERGGL LKPMISTID GQPTFLTGS AENQVTGGS SSPTPGRRQ RRSRHELRH LDEKELIFE LVKDICNEL EVRSLCHKI LQNVSTLLH ADRGSLFLV QGEKGGGCT PLPPGQTPR GRCLVSKLF DVCSRSTLT EMEKKEEIR IPWGTGIVG YVAESGEPV NIPDAYQDD RFNHDIDAL TGYRTKTLL CMPIKDTNG DVIGVAQVI NKVGDQPFT KQDEEVFAS YLQFCGIGL RNAHLYEKS QLEVKRNQV LLDLARMIF EEQSTIEHV VFRILTHTQ SLIQCQRVQ VLLVHQGSK ISFSRVFDF EANDLSAEE GESRTSPFE SRFPINVGI TGYVATTGE TVNIPVADE DDRFDPSVD DGTCFKHKT ILCMPIKNS LGQIIGVIQ LINKFNDLP FTKNDENFV EAFAIFCGM GIHNTHMYE KAVVAIAKH SVTLEVLSY HATASMEDA QRLRSLRVA SAAHFRLHD FAFDDINMN DDETLTACI RMFLDLDLV ERFHIDYEI LCRWLLSVK KNYRNVTYH NWRHAFNVA QMMFSILTA TQWWNIFGE IECLALMIA CLCHDLDHR GTNNSFQIK ASSPLAQLY STSTMEHHH FDQCIMILN SPGNQLLSN ISSEEYSRV IRVLEEAIL STDLAVYFR KRGAFFNVI RDRPCWSLD EHRELLRAM LMTVCDLAA ITKPWEVEK RVAELVSSE FFEQGDIER EELNITPID IMNREKEDQ LPLMQVGFI DSICLPIYE AFSRLSPQL EPLVEGVRK NRVKWLEIA AAQSLIPPE EQRDSNDNL SKPNTPESN PNASSQISD
- >TC005521 RA
atgtgtaaa ctcaatcat tgccgacat aagaaaagc ttgcatttg cgcagtggc ggcgacgac tgtcggatg ccggcagag gggggcgcc cctggctcg gattttgca caccgagat cgaccccat caaagaaac atcaaccat gcaggccat gggggccac tgctacgac cctgaatac gcccgaatg gaatcgtgg cttgacgaa catcccgat ttcgtacaa gactacttt ctcaggaaa gccaccaga caagtggtg gattcgtgg ctggtgtcg cacgcgacg ccgacgtcg tcgagcgtc gaattggcg tcaccgaca cacaaccag tccagggcc ggctcgggc gccacgacg cccgtccga aaaatttca gcgcatgag ttcgaacgt ggaggactt ctcaaaccg atgattagc accatcgat ggtcagccc acgtttctg acgggctct gcggaaaat caagtcacc gggggcagc agcagcccc acccccggc cggaggcag cggcgctcc aggcacgaa ctgaggcat ttggacgaa aaagaactg atttttgaa ctggtcaag gacatctgc aacgagctg gaggtccgt tcgttgtgc cacaagatc ctgcagaac gtctccacg ctgttgcac gccgaccga ggctccctt tttctcgtt caaggcgag aaaggcggt ggatgcacg ccgcttcca cccggacag acacctcga ggacggtgt cttgtctcg aaattattc gacgtctgc tccaggagt accctcaca gagatggaa aaaaaggag gaaattcgg ataccctgg ggcacgggg atcgtcgga tacgtcgcc gaaagtggc gaacctgtg aatataccg gacgcctac caggacgat cgattcaac cacgacatc gacgccttg acgggctac aggaccaag accttgctc tgcatgccc atcaaggac accaacggc gatgtcatc ggtgtggcg caagtgatc aacaaggtg ggcgaccag ccgttcacc aaacaagac gaagaagtc ttcgccagt tacctccag ttttgcgga atcggcctc cgcaatgcc catctgtac gaaaaatcg caactggag gtgaagcgc aatcaggtg cttttagac ctcgcccgc atgattttc gaagagcag agtaccatc gaacacgtc gttttcagg atactgacg cacacacag agtctgatt cagtgccag agggtgcag gttctcctg gtccaccag ggctcgaaa atcagcttt tcgcgcgtt ttcgacttc gaagcgaac gatctgagc gcagaagag ggcgaatcg cggaccagt cccttcgag agccgattt cccatcaat gtgggaatc accggttac gttgcgacg actggcgag acggtcaac ataccagtg gctgacgaa gacgaccgg tttgatccg agcgttgac gacggtact tgcttcaag cacaaaacc attctttgc atgcccata aaaaattcc ctcggccag atcattgga gtgattcaa ctgataaac aaattcaat gatttgccg ttcaccaaa aacgacgag aatttcgtc gaggctttc gccattttt tgcggcatg ggcattcac aacacgcac atgtatgag aaggccgtt gtggccatc gccaagcac agcgtcacc ctggaggtg ctgtcctat catgcgact gctagcatg gaggatgca cagaggcta aggagttta agggtggct tcggcggcc catttcagg ctccacgac ttcgccttt gacgacatc aacatgaat gacgatgag actctcact gcctgtatt agaatgttc ctcgatttg gatctagtc gaacgcttc cacatagat tacgaaatc ttgtgtagg tggttgtta tcagtgaaa aaaaattac agaaacgtt acttatcac aactggagg catgccttc aacgtggca caaatgatg ttttccata ttgaccgcc acacagtgg tggaacatt ttcggcgag atcgagtgt ttggccctg atgatagcg tgcctgtgc cacgacctg gaccatcga ggcaccaac aattcgttt caaatcaaa gcgtcctcg cctctcgcc cagttgtac tcaacgtcg acaatggag catcaccat ttcgaccaa tgtataatg attctgaac tcgcccgga aatcaattg ttgtcaaat atatcgtca gaggagtac tcgcgagtg attcgagtg cttgaagag gcgattcta tcgacagat ttggcggtt tactttcgc aaaagaggg gcctttttc aacgtgata agagatagg ccgtgctgg agtttggac gaacatcga gaactgttg agggcgatg ctgatgaca gtttgtgat ttggcggcc atcactaag ccctgggag gtcgagaag agagtggcc gaactggtg tcgtcggaa tttttcgaa cagggtgat atcgaacgg gaggaactc aacattact cccattgat ataatgaac agggaaaaa gaggaccaa ttaccccta atgcaagta ggattcatc gattcgatt tgcctgccg atctacgag gcgttctcg cggctgtcg ccgcagctg gagcccctg gtggagggt gtgcggaaa aaccgcgtg aagtggctc gagattgca gcagcacag agtcttatc ccccctgag gagcagcgc gactcaaac gacaacctc tccaagccg aacactccc gaatcgaat ccgaacgcc tcctcccaa ataagcgac tag
- >TC005521 RA
tcgcgcaca tcgtagcaa gccgcgata tttattttc agttgtttt gaaagcgcg acgcagata cgacggccg cgacgttcg tgaacgcga agcaggcca tgaatcgag tcgtcgcac actagatgg aaagtttcc gtagcagaa aatgttgtg ttagtgacg gaatttttc ctttttttt tgcggtgtg ttttatttg tggtggcat tctgtcagg aacggacct tgtggatga ttccgcgag gaaatgagc ctgaagaga gtccgcttg tgcgctaat aatttgaaa aagtggaag ggcagttag gcctcaaga cggcgtcgt caaatttca tccagaaga agaaccgga agaggctga aactgcagc tgttccggc ccgagttga aagaacagt ggccggtaa agaataata ataacaata ataagcagc ggaaaaaga acggcagcg ttctgggga aggtcagtg gcgtagcgg tgacgttga aacagtggt ggcgcgatg tggggctgt gccccaggg cagccccct cgacggtag gcggccccc tcgccgcga ggcatcgct gctgtcaca ctaagaggg gggccaacc gatccatgg gacaggcct tcagcatgt gtaaactca atcattgcc gacataaga aaagcttgc atttgcgca gtggcggcg acgactgtc ggatgccgg cagaggggg gcgcccctg gctcggatt ttgcacacc gagatcgac cccatcaaa gaaacatca accatgcag gccatgggg gccactgct acgaccctg aatacgccc gaatggaat cgtggcttg acgaacatc ccgatttcg tacaagact actttctca ggaaagcca ccagacaag tggtggatt cgtggctgg tgtcgcacg cgacgccga cgtcgtcga gcgtcgaat tggcgtcac cgacacaca accagtcca gggccggct cgggcgcca cgacgcccg tccgaaaaa tttcagcgc atgagttcg aacgtggag gacttctca aaccgatga ttagcacca tcgatggtc agcccacgt ttctgacgg gctctgcgg aaaatcaag tcaccgggg gcagcagca gccccaccc ccggccgga ggcagcggc gctccaggc acgaactga ggcatttgg acgaaaaag aactgattt ttgaactgg tcaaggaca tctgcaacg agctggagg tccgttcgt tgtgccaca agatcctgc agaacgtct ccacgctgt tgcacgccg accgaggct ccctttttc tcgttcaag gcgagaaag gcggtggat gcacgccgc ttccacccg gacagacac ctcgaggac ggtgtcttg tctcgaaat tattcgacg tctgctcca ggagtaccc tcacagaga tggaaaaaa aggaggaaa ttcggatac cctggggca cggggatcg tcggatacg tcgccgaaa gtggcgaac ctgtgaata taccggacg cctaccagg acgatcgat tcaaccacg acatcgacg ccttgacgg gctacagga ccaagacct tgctctgca tgcccatca aggacacca acggcgatg tcatcggtg tggcgcaag tgatcaaca aggtgggcg accagccgt tcaccaaac aagacgaag aagtcttcg ccagttacc tccagtttt gcggaatcg gcctccgca atgcccatc tgtacgaaa aatcgcaac tggaggtga agcgcaatc aggtgcttt tagacctcg cccgcatga ttttcgaag agcagagta ccatcgaac acgtcgttt tcaggatac tgacgcaca cacagagtc tgattcagt gccagaggg tgcaggttc tcctggtcc accagggct cgaaaatca gcttttcgc gcgttttcg acttcgaag cgaacgatc tgagcgcag aagagggcg aatcgcgga ccagtccct tcgagagcc gatttccca tcaatgtgg gaatcaccg gttacgttg cgacgactg gcgagacgg tcaacatac cagtggctg acgaagacg accggtttg atccgagcg ttgacgacg gtacttgct tcaagcaca aaaccattc tttgcatgc ccataaaaa attccctcg gccagatca ttggagtga ttcaactga taaacaaat tcaatgatt tgccgttca ccaaaaacg acgagaatt tcgtcgagg ctttcgcca ttttttgcg gcatgggca ttcacaaca cgcacatgt atgagaagg ccgttgtgg ccatcgcca agcacagcg tcaccctgg aggtgctgt cctatcatg cgactgcta gcatggagg atgcacaga ggctaagga gtttaaggg tggcttcgg cggcccatt tcaggctcc acgacttcg cctttgacg acatcaaca tgaatgacg atgagactc tcactgcct gtattagaa tgttcctcg atttggatc tagtcgaac gcttccaca tagattacg aaatcttgt gtaggtggt tgttatcag tgaaaaaaa attacagaa acgttactt atcacaact ggaggcatg ccttcaacg tggcacaaa tgatgtttt ccatattga ccgccacac agtggtgga acattttcg gcgagatcg agtgtttgg ccctgatga tagcgtgcc tgtgccacg acctggacc atcgaggca ccaacaatt cgtttcaaa tcaaagcgt cctcgcctc tcgcccagt tgtactcaa cgtcgacaa tggagcatc accatttcg accaatgta taatgattc tgaactcgc ccggaaatc aattgttgt caaatatat cgtcagagg agtactcgc gagtgattc gagtgcttg aagaggcga ttctatcga cagatttgg cggtttact ttcgcaaaa gaggggcct ttttcaacg tgataagag ataggccgt gctggagtt tggacgaac atcgagaac tgttgaggg cgatgctga tgacagttt gtgatttgg cggccatca ctaagccct gggaggtcg agaagagag tggccgaac tggtgtcgt cggaatttt tcgaacagg gtgatatcg aacgggagg aactcaaca ttactccca ttgatataa tgaacaggg aaaaagagg accaattac ccctaatgc aagtaggat tcatcgatt cgatttgcc tgccgatct acgaggcgt tctcgcggc tgtcgccgc agctggagc ccctggtgg agggtgtgc ggaaaaacc gcgtgaagt ggctcgaga ttgcagcag cacagagtc ttatccccc ctgaggagc agcgcgact caaacgaca acctctcca agccgaaca ctcccgaat cgaatccga acgcctcct cccaaataa gcgactagt aaattgtag ttattgtaa ataattccc gaagactat ttattctag tttaaatat aatttgaaa gacttaata atgacgacc acggtgttt taagagagt gttattttt ttaaagtat tgtcaaaga attcttcaa ggctctaaa acttccctc cctgtatta atttattta tttccgcga aaatctcag ttttattta ttatttgtg agtattgca taggttgta agagagctc tcttaagtt tctaggcta ggtttttag tagtcactt gttgtgata ttttagtgg gctctacac aaatatggc aagtagagg accgtattt taatatggt attatattc gttgaggaa actatttat accaaagcg tatttttta atcctattt tactaatta agttcatgt ttttaatgt ctatcattt cgcgacaac tgttttcga aaattgttt tctatcaaa tttactaag tgtacattc tgtgtaaaa taataatta caataacaa tattaatat aataaacac ttaccgaaa ga
