Status
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Gene information
General information
TC005521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11802440..11827163
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005521
    iBeetle screen iB_00875

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Adult morphology
    adult size decreased - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005521 RA
      MCKLNHCRH KKSLHLRSG GDDCRMPAE GGAPGSDFA 
      HRDRPHQRN INHAGHGGH CYDPEYARM ESWLDEHPD 
      FVQDYFLRK ATRQVVDSW LVSHATPTS SSVELASPT 
      HNQSRAGSG ATTPVRKIS AHEFERGGL LKPMISTID 
      GQPTFLTGS AENQVTGGS SSPTPGRRQ RRSRHELRH 
      LDEKELIFE LVKDICNEL EVRSLCHKI LQNVSTLLH 
      ADRGSLFLV QGEKGGGCT PLPPGQTPR GRCLVSKLF 
      DVCSRSTLT EMEKKEEIR IPWGTGIVG YVAESGEPV 
      NIPDAYQDD RFNHDIDAL TGYRTKTLL CMPIKDTNG 
      DVIGVAQVI NKVGDQPFT KQDEEVFAS YLQFCGIGL 
      RNAHLYEKS QLEVKRNQV LLDLARMIF EEQSTIEHV 
      VFRILTHTQ SLIQCQRVQ VLLVHQGSK ISFSRVFDF 
      EANDLSAEE GESRTSPFE SRFPINVGI TGYVATTGE 
      TVNIPVADE DDRFDPSVD DGTCFKHKT ILCMPIKNS 
      LGQIIGVIQ LINKFNDLP FTKNDENFV EAFAIFCGM 
      GIHNTHMYE KAVVAIAKH SVTLEVLSY HATASMEDA 
      QRLRSLRVA SAAHFRLHD FAFDDINMN DDETLTACI 
      RMFLDLDLV ERFHIDYEI LCRWLLSVK KNYRNVTYH 
      NWRHAFNVA QMMFSILTA TQWWNIFGE IECLALMIA 
      CLCHDLDHR GTNNSFQIK ASSPLAQLY STSTMEHHH 
      FDQCIMILN SPGNQLLSN ISSEEYSRV IRVLEEAIL 
      STDLAVYFR KRGAFFNVI RDRPCWSLD EHRELLRAM 
      LMTVCDLAA ITKPWEVEK RVAELVSSE FFEQGDIER 
      EELNITPID IMNREKEDQ LPLMQVGFI DSICLPIYE 
      AFSRLSPQL EPLVEGVRK NRVKWLEIA AAQSLIPPE 
      EQRDSNDNL SKPNTPESN PNASSQISD
    CDS sequence
    • >TC005521 RA
      atgtgtaaa ctcaatcat tgccgacat aagaaaagc 
      ttgcatttg cgcagtggc ggcgacgac tgtcggatg 
      ccggcagag gggggcgcc cctggctcg gattttgca 
      caccgagat cgaccccat caaagaaac atcaaccat 
      gcaggccat gggggccac tgctacgac cctgaatac 
      gcccgaatg gaatcgtgg cttgacgaa catcccgat 
      ttcgtacaa gactacttt ctcaggaaa gccaccaga 
      caagtggtg gattcgtgg ctggtgtcg cacgcgacg 
      ccgacgtcg tcgagcgtc gaattggcg tcaccgaca 
      cacaaccag tccagggcc ggctcgggc gccacgacg 
      cccgtccga aaaatttca gcgcatgag ttcgaacgt 
      ggaggactt ctcaaaccg atgattagc accatcgat 
      ggtcagccc acgtttctg acgggctct gcggaaaat 
      caagtcacc gggggcagc agcagcccc acccccggc 
      cggaggcag cggcgctcc aggcacgaa ctgaggcat 
      ttggacgaa aaagaactg atttttgaa ctggtcaag 
      gacatctgc aacgagctg gaggtccgt tcgttgtgc 
      cacaagatc ctgcagaac gtctccacg ctgttgcac 
      gccgaccga ggctccctt tttctcgtt caaggcgag 
      aaaggcggt ggatgcacg ccgcttcca cccggacag 
      acacctcga ggacggtgt cttgtctcg aaattattc 
      gacgtctgc tccaggagt accctcaca gagatggaa 
      aaaaaggag gaaattcgg ataccctgg ggcacgggg 
      atcgtcgga tacgtcgcc gaaagtggc gaacctgtg 
      aatataccg gacgcctac caggacgat cgattcaac 
      cacgacatc gacgccttg acgggctac aggaccaag 
      accttgctc tgcatgccc atcaaggac accaacggc 
      gatgtcatc ggtgtggcg caagtgatc aacaaggtg 
      ggcgaccag ccgttcacc aaacaagac gaagaagtc 
      ttcgccagt tacctccag ttttgcgga atcggcctc 
      cgcaatgcc catctgtac gaaaaatcg caactggag 
      gtgaagcgc aatcaggtg cttttagac ctcgcccgc 
      atgattttc gaagagcag agtaccatc gaacacgtc 
      gttttcagg atactgacg cacacacag agtctgatt 
      cagtgccag agggtgcag gttctcctg gtccaccag 
      ggctcgaaa atcagcttt tcgcgcgtt ttcgacttc 
      gaagcgaac gatctgagc gcagaagag ggcgaatcg 
      cggaccagt cccttcgag agccgattt cccatcaat 
      gtgggaatc accggttac gttgcgacg actggcgag 
      acggtcaac ataccagtg gctgacgaa gacgaccgg 
      tttgatccg agcgttgac gacggtact tgcttcaag 
      cacaaaacc attctttgc atgcccata aaaaattcc 
      ctcggccag atcattgga gtgattcaa ctgataaac 
      aaattcaat gatttgccg ttcaccaaa aacgacgag 
      aatttcgtc gaggctttc gccattttt tgcggcatg 
      ggcattcac aacacgcac atgtatgag aaggccgtt 
      gtggccatc gccaagcac agcgtcacc ctggaggtg 
      ctgtcctat catgcgact gctagcatg gaggatgca 
      cagaggcta aggagttta agggtggct tcggcggcc 
      catttcagg ctccacgac ttcgccttt gacgacatc 
      aacatgaat gacgatgag actctcact gcctgtatt 
      agaatgttc ctcgatttg gatctagtc gaacgcttc 
      cacatagat tacgaaatc ttgtgtagg tggttgtta 
      tcagtgaaa aaaaattac agaaacgtt acttatcac 
      aactggagg catgccttc aacgtggca caaatgatg 
      ttttccata ttgaccgcc acacagtgg tggaacatt 
      ttcggcgag atcgagtgt ttggccctg atgatagcg 
      tgcctgtgc cacgacctg gaccatcga ggcaccaac 
      aattcgttt caaatcaaa gcgtcctcg cctctcgcc 
      cagttgtac tcaacgtcg acaatggag catcaccat 
      ttcgaccaa tgtataatg attctgaac tcgcccgga 
      aatcaattg ttgtcaaat atatcgtca gaggagtac 
      tcgcgagtg attcgagtg cttgaagag gcgattcta 
      tcgacagat ttggcggtt tactttcgc aaaagaggg 
      gcctttttc aacgtgata agagatagg ccgtgctgg 
      agtttggac gaacatcga gaactgttg agggcgatg 
      ctgatgaca gtttgtgat ttggcggcc atcactaag 
      ccctgggag gtcgagaag agagtggcc gaactggtg 
      tcgtcggaa tttttcgaa cagggtgat atcgaacgg 
      gaggaactc aacattact cccattgat ataatgaac 
      agggaaaaa gaggaccaa ttaccccta atgcaagta 
      ggattcatc gattcgatt tgcctgccg atctacgag 
      gcgttctcg cggctgtcg ccgcagctg gagcccctg 
      gtggagggt gtgcggaaa aaccgcgtg aagtggctc 
      gagattgca gcagcacag agtcttatc ccccctgag 
      gagcagcgc gactcaaac gacaacctc tccaagccg 
      aacactccc gaatcgaat ccgaacgcc tcctcccaa 
      ataagcgac tag
    mRNA sequence
    • >TC005521 RA
      tcgcgcaca tcgtagcaa gccgcgata tttattttc 
      agttgtttt gaaagcgcg acgcagata cgacggccg 
      cgacgttcg tgaacgcga agcaggcca tgaatcgag 
      tcgtcgcac actagatgg aaagtttcc gtagcagaa 
      aatgttgtg ttagtgacg gaatttttc ctttttttt 
      tgcggtgtg ttttatttg tggtggcat tctgtcagg 
      aacggacct tgtggatga ttccgcgag gaaatgagc 
      ctgaagaga gtccgcttg tgcgctaat aatttgaaa 
      aagtggaag ggcagttag gcctcaaga cggcgtcgt 
      caaatttca tccagaaga agaaccgga agaggctga 
      aactgcagc tgttccggc ccgagttga aagaacagt 
      ggccggtaa agaataata ataacaata ataagcagc 
      ggaaaaaga acggcagcg ttctgggga aggtcagtg 
      gcgtagcgg tgacgttga aacagtggt ggcgcgatg 
      tggggctgt gccccaggg cagccccct cgacggtag 
      gcggccccc tcgccgcga ggcatcgct gctgtcaca 
      ctaagaggg gggccaacc gatccatgg gacaggcct 
      tcagcatgt gtaaactca atcattgcc gacataaga 
      aaagcttgc atttgcgca gtggcggcg acgactgtc 
      ggatgccgg cagaggggg gcgcccctg gctcggatt 
      ttgcacacc gagatcgac cccatcaaa gaaacatca 
      accatgcag gccatgggg gccactgct acgaccctg 
      aatacgccc gaatggaat cgtggcttg acgaacatc 
      ccgatttcg tacaagact actttctca ggaaagcca 
      ccagacaag tggtggatt cgtggctgg tgtcgcacg 
      cgacgccga cgtcgtcga gcgtcgaat tggcgtcac 
      cgacacaca accagtcca gggccggct cgggcgcca 
      cgacgcccg tccgaaaaa tttcagcgc atgagttcg 
      aacgtggag gacttctca aaccgatga ttagcacca 
      tcgatggtc agcccacgt ttctgacgg gctctgcgg 
      aaaatcaag tcaccgggg gcagcagca gccccaccc 
      ccggccgga ggcagcggc gctccaggc acgaactga 
      ggcatttgg acgaaaaag aactgattt ttgaactgg 
      tcaaggaca tctgcaacg agctggagg tccgttcgt 
      tgtgccaca agatcctgc agaacgtct ccacgctgt 
      tgcacgccg accgaggct ccctttttc tcgttcaag 
      gcgagaaag gcggtggat gcacgccgc ttccacccg 
      gacagacac ctcgaggac ggtgtcttg tctcgaaat 
      tattcgacg tctgctcca ggagtaccc tcacagaga 
      tggaaaaaa aggaggaaa ttcggatac cctggggca 
      cggggatcg tcggatacg tcgccgaaa gtggcgaac 
      ctgtgaata taccggacg cctaccagg acgatcgat 
      tcaaccacg acatcgacg ccttgacgg gctacagga 
      ccaagacct tgctctgca tgcccatca aggacacca 
      acggcgatg tcatcggtg tggcgcaag tgatcaaca 
      aggtgggcg accagccgt tcaccaaac aagacgaag 
      aagtcttcg ccagttacc tccagtttt gcggaatcg 
      gcctccgca atgcccatc tgtacgaaa aatcgcaac 
      tggaggtga agcgcaatc aggtgcttt tagacctcg 
      cccgcatga ttttcgaag agcagagta ccatcgaac 
      acgtcgttt tcaggatac tgacgcaca cacagagtc 
      tgattcagt gccagaggg tgcaggttc tcctggtcc 
      accagggct cgaaaatca gcttttcgc gcgttttcg 
      acttcgaag cgaacgatc tgagcgcag aagagggcg 
      aatcgcgga ccagtccct tcgagagcc gatttccca 
      tcaatgtgg gaatcaccg gttacgttg cgacgactg 
      gcgagacgg tcaacatac cagtggctg acgaagacg 
      accggtttg atccgagcg ttgacgacg gtacttgct 
      tcaagcaca aaaccattc tttgcatgc ccataaaaa 
      attccctcg gccagatca ttggagtga ttcaactga 
      taaacaaat tcaatgatt tgccgttca ccaaaaacg 
      acgagaatt tcgtcgagg ctttcgcca ttttttgcg 
      gcatgggca ttcacaaca cgcacatgt atgagaagg 
      ccgttgtgg ccatcgcca agcacagcg tcaccctgg 
      aggtgctgt cctatcatg cgactgcta gcatggagg 
      atgcacaga ggctaagga gtttaaggg tggcttcgg 
      cggcccatt tcaggctcc acgacttcg cctttgacg 
      acatcaaca tgaatgacg atgagactc tcactgcct 
      gtattagaa tgttcctcg atttggatc tagtcgaac 
      gcttccaca tagattacg aaatcttgt gtaggtggt 
      tgttatcag tgaaaaaaa attacagaa acgttactt 
      atcacaact ggaggcatg ccttcaacg tggcacaaa 
      tgatgtttt ccatattga ccgccacac agtggtgga 
      acattttcg gcgagatcg agtgtttgg ccctgatga 
      tagcgtgcc tgtgccacg acctggacc atcgaggca 
      ccaacaatt cgtttcaaa tcaaagcgt cctcgcctc 
      tcgcccagt tgtactcaa cgtcgacaa tggagcatc 
      accatttcg accaatgta taatgattc tgaactcgc 
      ccggaaatc aattgttgt caaatatat cgtcagagg 
      agtactcgc gagtgattc gagtgcttg aagaggcga 
      ttctatcga cagatttgg cggtttact ttcgcaaaa 
      gaggggcct ttttcaacg tgataagag ataggccgt 
      gctggagtt tggacgaac atcgagaac tgttgaggg 
      cgatgctga tgacagttt gtgatttgg cggccatca 
      ctaagccct gggaggtcg agaagagag tggccgaac 
      tggtgtcgt cggaatttt tcgaacagg gtgatatcg 
      aacgggagg aactcaaca ttactccca ttgatataa 
      tgaacaggg aaaaagagg accaattac ccctaatgc 
      aagtaggat tcatcgatt cgatttgcc tgccgatct 
      acgaggcgt tctcgcggc tgtcgccgc agctggagc 
      ccctggtgg agggtgtgc ggaaaaacc gcgtgaagt 
      ggctcgaga ttgcagcag cacagagtc ttatccccc 
      ctgaggagc agcgcgact caaacgaca acctctcca 
      agccgaaca ctcccgaat cgaatccga acgcctcct 
      cccaaataa gcgactagt aaattgtag ttattgtaa 
      ataattccc gaagactat ttattctag tttaaatat 
      aatttgaaa gacttaata atgacgacc acggtgttt 
      taagagagt gttattttt ttaaagtat tgtcaaaga 
      attcttcaa ggctctaaa acttccctc cctgtatta 
      atttattta tttccgcga aaatctcag ttttattta 
      ttatttgtg agtattgca taggttgta agagagctc 
      tcttaagtt tctaggcta ggtttttag tagtcactt 
      gttgtgata ttttagtgg gctctacac aaatatggc 
      aagtagagg accgtattt taatatggt attatattc 
      gttgaggaa actatttat accaaagcg tatttttta 
      atcctattt tactaatta agttcatgt ttttaatgt 
      ctatcattt cgcgacaac tgttttcga aaattgttt 
      tctatcaaa tttactaag tgtacattc tgtgtaaaa 
      taataatta caataacaa tattaatat aataaacac 
      ttaccgaaa ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY