Status
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Gene information
General information
TC005521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11802440..11827163
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005521
    iBeetle screen iB_00875

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    Adult morphology
    adult size decreased - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005521 RA
      MCKLNHCRH KKSLHLRSG GDDCRMPAE GGAPGSDFA 
HRDRPHQRN INHAGHGGH CYDPEYARM ESWLDEHPD 
FVQDYFLRK ATRQVVDSW LVSHATPTS SSVELASPT 
HNQSRAGSG ATTPVRKIS AHEFERGGL LKPMISTID 
GQPTFLTGS AENQVTGGS SSPTPGRRQ RRSRHELRH 
LDEKELIFE LVKDICNEL EVRSLCHKI LQNVSTLLH 
ADRGSLFLV QGEKGGGCT PLPPGQTPR GRCLVSKLF 
DVCSRSTLT EMEKKEEIR IPWGTGIVG YVAESGEPV 
NIPDAYQDD RFNHDIDAL TGYRTKTLL CMPIKDTNG 
DVIGVAQVI NKVGDQPFT KQDEEVFAS YLQFCGIGL 
RNAHLYEKS QLEVKRNQV LLDLARMIF EEQSTIEHV 
VFRILTHTQ SLIQCQRVQ VLLVHQGSK ISFSRVFDF 
EANDLSAEE GESRTSPFE SRFPINVGI TGYVATTGE 
TVNIPVADE DDRFDPSVD DGTCFKHKT ILCMPIKNS 
LGQIIGVIQ LINKFNDLP FTKNDENFV EAFAIFCGM 
GIHNTHMYE KAVVAIAKH SVTLEVLSY HATASMEDA 
QRLRSLRVA SAAHFRLHD FAFDDINMN DDETLTACI 
RMFLDLDLV ERFHIDYEI LCRWLLSVK KNYRNVTYH 
NWRHAFNVA QMMFSILTA TQWWNIFGE IECLALMIA 
CLCHDLDHR GTNNSFQIK ASSPLAQLY STSTMEHHH 
FDQCIMILN SPGNQLLSN ISSEEYSRV IRVLEEAIL 
STDLAVYFR KRGAFFNVI RDRPCWSLD EHRELLRAM 
LMTVCDLAA ITKPWEVEK RVAELVSSE FFEQGDIER 
EELNITPID IMNREKEDQ LPLMQVGFI DSICLPIYE 
AFSRLSPQL EPLVEGVRK NRVKWLEIA AAQSLIPPE 
EQRDSNDNL SKPNTPESN PNASSQISD
    CDS sequence
    • >TC005521 RA
      atgtgtaaa ctcaatcat tgccgacat aagaaaagc 
ttgcatttg cgcagtggc ggcgacgac tgtcggatg 
ccggcagag gggggcgcc cctggctcg gattttgca 
caccgagat cgaccccat caaagaaac atcaaccat 
gcaggccat gggggccac tgctacgac cctgaatac 
gcccgaatg gaatcgtgg cttgacgaa catcccgat 
ttcgtacaa gactacttt ctcaggaaa gccaccaga 
caagtggtg gattcgtgg ctggtgtcg cacgcgacg 
ccgacgtcg tcgagcgtc gaattggcg tcaccgaca 
cacaaccag tccagggcc ggctcgggc gccacgacg 
cccgtccga aaaatttca gcgcatgag ttcgaacgt 
ggaggactt ctcaaaccg atgattagc accatcgat 
ggtcagccc acgtttctg acgggctct gcggaaaat 
caagtcacc gggggcagc agcagcccc acccccggc 
cggaggcag cggcgctcc aggcacgaa ctgaggcat 
ttggacgaa aaagaactg atttttgaa ctggtcaag 
gacatctgc aacgagctg gaggtccgt tcgttgtgc 
cacaagatc ctgcagaac gtctccacg ctgttgcac 
gccgaccga ggctccctt tttctcgtt caaggcgag 
aaaggcggt ggatgcacg ccgcttcca cccggacag 
acacctcga ggacggtgt cttgtctcg aaattattc 
gacgtctgc tccaggagt accctcaca gagatggaa 
aaaaaggag gaaattcgg ataccctgg ggcacgggg 
atcgtcgga tacgtcgcc gaaagtggc gaacctgtg 
aatataccg gacgcctac caggacgat cgattcaac 
cacgacatc gacgccttg acgggctac aggaccaag 
accttgctc tgcatgccc atcaaggac accaacggc 
gatgtcatc ggtgtggcg caagtgatc aacaaggtg 
ggcgaccag ccgttcacc aaacaagac gaagaagtc 
ttcgccagt tacctccag ttttgcgga atcggcctc 
cgcaatgcc catctgtac gaaaaatcg caactggag 
gtgaagcgc aatcaggtg cttttagac ctcgcccgc 
atgattttc gaagagcag agtaccatc gaacacgtc 
gttttcagg atactgacg cacacacag agtctgatt 
cagtgccag agggtgcag gttctcctg gtccaccag 
ggctcgaaa atcagcttt tcgcgcgtt ttcgacttc 
gaagcgaac gatctgagc gcagaagag ggcgaatcg 
cggaccagt cccttcgag agccgattt cccatcaat 
gtgggaatc accggttac gttgcgacg actggcgag 
acggtcaac ataccagtg gctgacgaa gacgaccgg 
tttgatccg agcgttgac gacggtact tgcttcaag 
cacaaaacc attctttgc atgcccata aaaaattcc 
ctcggccag atcattgga gtgattcaa ctgataaac 
aaattcaat gatttgccg ttcaccaaa aacgacgag 
aatttcgtc gaggctttc gccattttt tgcggcatg 
ggcattcac aacacgcac atgtatgag aaggccgtt 
gtggccatc gccaagcac agcgtcacc ctggaggtg 
ctgtcctat catgcgact gctagcatg gaggatgca 
cagaggcta aggagttta agggtggct tcggcggcc 
catttcagg ctccacgac ttcgccttt gacgacatc 
aacatgaat gacgatgag actctcact gcctgtatt 
agaatgttc ctcgatttg gatctagtc gaacgcttc 
cacatagat tacgaaatc ttgtgtagg tggttgtta 
tcagtgaaa aaaaattac agaaacgtt acttatcac 
aactggagg catgccttc aacgtggca caaatgatg 
ttttccata ttgaccgcc acacagtgg tggaacatt 
ttcggcgag atcgagtgt ttggccctg atgatagcg 
tgcctgtgc cacgacctg gaccatcga ggcaccaac 
aattcgttt caaatcaaa gcgtcctcg cctctcgcc 
cagttgtac tcaacgtcg acaatggag catcaccat 
ttcgaccaa tgtataatg attctgaac tcgcccgga 
aatcaattg ttgtcaaat atatcgtca gaggagtac 
tcgcgagtg attcgagtg cttgaagag gcgattcta 
tcgacagat ttggcggtt tactttcgc aaaagaggg 
gcctttttc aacgtgata agagatagg ccgtgctgg 
agtttggac gaacatcga gaactgttg agggcgatg 
ctgatgaca gtttgtgat ttggcggcc atcactaag 
ccctgggag gtcgagaag agagtggcc gaactggtg 
tcgtcggaa tttttcgaa cagggtgat atcgaacgg 
gaggaactc aacattact cccattgat ataatgaac 
agggaaaaa gaggaccaa ttaccccta atgcaagta 
ggattcatc gattcgatt tgcctgccg atctacgag 
gcgttctcg cggctgtcg ccgcagctg gagcccctg 
gtggagggt gtgcggaaa aaccgcgtg aagtggctc 
gagattgca gcagcacag agtcttatc ccccctgag 
gagcagcgc gactcaaac gacaacctc tccaagccg 
aacactccc gaatcgaat ccgaacgcc tcctcccaa 
ataagcgac tag
    mRNA sequence
    • >TC005521 RA
      tcgcgcaca tcgtagcaa gccgcgata tttattttc 
agttgtttt gaaagcgcg acgcagata cgacggccg 
cgacgttcg tgaacgcga agcaggcca tgaatcgag 
tcgtcgcac actagatgg aaagtttcc gtagcagaa 
aatgttgtg ttagtgacg gaatttttc ctttttttt 
tgcggtgtg ttttatttg tggtggcat tctgtcagg 
aacggacct tgtggatga ttccgcgag gaaatgagc 
ctgaagaga gtccgcttg tgcgctaat aatttgaaa 
aagtggaag ggcagttag gcctcaaga cggcgtcgt 
caaatttca tccagaaga agaaccgga agaggctga 
aactgcagc tgttccggc ccgagttga aagaacagt 
ggccggtaa agaataata ataacaata ataagcagc 
ggaaaaaga acggcagcg ttctgggga aggtcagtg 
gcgtagcgg tgacgttga aacagtggt ggcgcgatg 
tggggctgt gccccaggg cagccccct cgacggtag 
gcggccccc tcgccgcga ggcatcgct gctgtcaca 
ctaagaggg gggccaacc gatccatgg gacaggcct 
tcagcatgt gtaaactca atcattgcc gacataaga 
aaagcttgc atttgcgca gtggcggcg acgactgtc 
ggatgccgg cagaggggg gcgcccctg gctcggatt 
ttgcacacc gagatcgac cccatcaaa gaaacatca 
accatgcag gccatgggg gccactgct acgaccctg 
aatacgccc gaatggaat cgtggcttg acgaacatc 
ccgatttcg tacaagact actttctca ggaaagcca 
ccagacaag tggtggatt cgtggctgg tgtcgcacg 
cgacgccga cgtcgtcga gcgtcgaat tggcgtcac 
cgacacaca accagtcca gggccggct cgggcgcca 
cgacgcccg tccgaaaaa tttcagcgc atgagttcg 
aacgtggag gacttctca aaccgatga ttagcacca 
tcgatggtc agcccacgt ttctgacgg gctctgcgg 
aaaatcaag tcaccgggg gcagcagca gccccaccc 
ccggccgga ggcagcggc gctccaggc acgaactga 
ggcatttgg acgaaaaag aactgattt ttgaactgg 
tcaaggaca tctgcaacg agctggagg tccgttcgt 
tgtgccaca agatcctgc agaacgtct ccacgctgt 
tgcacgccg accgaggct ccctttttc tcgttcaag 
gcgagaaag gcggtggat gcacgccgc ttccacccg 
gacagacac ctcgaggac ggtgtcttg tctcgaaat 
tattcgacg tctgctcca ggagtaccc tcacagaga 
tggaaaaaa aggaggaaa ttcggatac cctggggca 
cggggatcg tcggatacg tcgccgaaa gtggcgaac 
ctgtgaata taccggacg cctaccagg acgatcgat 
tcaaccacg acatcgacg ccttgacgg gctacagga 
ccaagacct tgctctgca tgcccatca aggacacca 
acggcgatg tcatcggtg tggcgcaag tgatcaaca 
aggtgggcg accagccgt tcaccaaac aagacgaag 
aagtcttcg ccagttacc tccagtttt gcggaatcg 
gcctccgca atgcccatc tgtacgaaa aatcgcaac 
tggaggtga agcgcaatc aggtgcttt tagacctcg 
cccgcatga ttttcgaag agcagagta ccatcgaac 
acgtcgttt tcaggatac tgacgcaca cacagagtc 
tgattcagt gccagaggg tgcaggttc tcctggtcc 
accagggct cgaaaatca gcttttcgc gcgttttcg 
acttcgaag cgaacgatc tgagcgcag aagagggcg 
aatcgcgga ccagtccct tcgagagcc gatttccca 
tcaatgtgg gaatcaccg gttacgttg cgacgactg 
gcgagacgg tcaacatac cagtggctg acgaagacg 
accggtttg atccgagcg ttgacgacg gtacttgct 
tcaagcaca aaaccattc tttgcatgc ccataaaaa 
attccctcg gccagatca ttggagtga ttcaactga 
taaacaaat tcaatgatt tgccgttca ccaaaaacg 
acgagaatt tcgtcgagg ctttcgcca ttttttgcg 
gcatgggca ttcacaaca cgcacatgt atgagaagg 
ccgttgtgg ccatcgcca agcacagcg tcaccctgg 
aggtgctgt cctatcatg cgactgcta gcatggagg 
atgcacaga ggctaagga gtttaaggg tggcttcgg 
cggcccatt tcaggctcc acgacttcg cctttgacg 
acatcaaca tgaatgacg atgagactc tcactgcct 
gtattagaa tgttcctcg atttggatc tagtcgaac 
gcttccaca tagattacg aaatcttgt gtaggtggt 
tgttatcag tgaaaaaaa attacagaa acgttactt 
atcacaact ggaggcatg ccttcaacg tggcacaaa 
tgatgtttt ccatattga ccgccacac agtggtgga 
acattttcg gcgagatcg agtgtttgg ccctgatga 
tagcgtgcc tgtgccacg acctggacc atcgaggca 
ccaacaatt cgtttcaaa tcaaagcgt cctcgcctc 
tcgcccagt tgtactcaa cgtcgacaa tggagcatc 
accatttcg accaatgta taatgattc tgaactcgc 
ccggaaatc aattgttgt caaatatat cgtcagagg 
agtactcgc gagtgattc gagtgcttg aagaggcga 
ttctatcga cagatttgg cggtttact ttcgcaaaa 
gaggggcct ttttcaacg tgataagag ataggccgt 
gctggagtt tggacgaac atcgagaac tgttgaggg 
cgatgctga tgacagttt gtgatttgg cggccatca 
ctaagccct gggaggtcg agaagagag tggccgaac 
tggtgtcgt cggaatttt tcgaacagg gtgatatcg 
aacgggagg aactcaaca ttactccca ttgatataa 
tgaacaggg aaaaagagg accaattac ccctaatgc 
aagtaggat tcatcgatt cgatttgcc tgccgatct 
acgaggcgt tctcgcggc tgtcgccgc agctggagc 
ccctggtgg agggtgtgc ggaaaaacc gcgtgaagt 
ggctcgaga ttgcagcag cacagagtc ttatccccc 
ctgaggagc agcgcgact caaacgaca acctctcca 
agccgaaca ctcccgaat cgaatccga acgcctcct 
cccaaataa gcgactagt aaattgtag ttattgtaa 
ataattccc gaagactat ttattctag tttaaatat 
aatttgaaa gacttaata atgacgacc acggtgttt 
taagagagt gttattttt ttaaagtat tgtcaaaga 
attcttcaa ggctctaaa acttccctc cctgtatta 
atttattta tttccgcga aaatctcag ttttattta 
ttatttgtg agtattgca taggttgta agagagctc 
tcttaagtt tctaggcta ggtttttag tagtcactt 
gttgtgata ttttagtgg gctctacac aaatatggc 
aagtagagg accgtattt taatatggt attatattc 
gttgaggaa actatttat accaaagcg tatttttta 
atcctattt tactaatta agttcatgt ttttaatgt 
ctatcattt cgcgacaac tgttttcga aaattgttt 
tctatcaaa tttactaag tgtacattc tgtgtaaaa 
taataatta caataacaa tattaatat aataaacac 
ttaccgaaa ga
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