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Gene information
General information
TC005515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11860686..11863403
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005515
    iBeetle screen iB_00872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005515 RA
      MADQSNQVR RPMLSIDLP NSVNSIGGF RPLPSQPGA 
      TSERLGAAA GTNRPKMSL PTISKSTVD PTIDRTRFG 
      PSERNKQQR DSRFKICQS MNSSGKLQI GGKTYDFTS 
      DDLQDIGEI GRGGFGTVN KMVHRKSGT VVAVKRIRS 
      TVDEKEQKQ LLMDLDVVM KSNECHYIV QFYGALFKE 
      GDCWIVMEL MDTSLDKFY KFIYDKLGD RIPEEILGK 
      IALATVKAL NYLKEKLKI IHRDVKPSN ILLDRKGNI 
      KLCDFGISG QLVDSIART RDAGCRPYM APERIDPQT 
      AKGYDVRSD VWSLGITLM EVATGRFPY PRWSSVFDQ 
      LHQVVNGEP PRLTENHNA NTFTSEFVN FVNTCLIKD 
      EHSRPKYKR LLEDPFILR AQKEKVDVA AYVSRIMDE 
      MANNGISAF TTNQQ
    • >TC005515 RB
      MADQSNQVR RPMLSIDLP NSVNSIGGF RPLPSQPGA 
      TSERLGAAA GTNRPKMSL PTISKSTVD PTIDRTRDS 
      RFKICQSMN SSGKLQIGG KTYDFTSDD LQDIGEIGR 
      GGFGTVNKM VHRKSGTVV AVKRIRSTV DEKEQKQLL 
      MDLDVVMKS NECHYIVQF YGALFKEGD CWIVMELMD 
      TSLDKFYKF IYDKLGDRI PEEILGKIA LATVKALNY 
      LKEKLKIIH RDVKPSNIL LDRKGNIKL CDFGISGQL 
      VDSIARTRD AGCRPYMAP ERIDPQTAK GYDVRSDVW 
      SLGITLMEV ATGRFPYPR WSSVFDQLH QVVNGEPPR 
      LTENHNANT FTSEFVNFV NTCLIKDEH SRPKYKRLL 
      EDPFILRAQ KEKVDVAAY VSRIMDEMA NNGISAFTT 
      NQQ
    CDS sequence
    • >TC005515 RA
      atggcagat cagtccaat caagtccgg aggcctatg 
      ctgagcata gacttgccc aacagtgtg aattccatc 
      gggggcttt cgcccgctg cccagccag ccgggggcc 
      accagcgag cgtttgggg gccgcagcc gggacaaac 
      cgccccaaa atgagcctc ccgaccatc tccaagtcc 
      acagtcgat cccacgatt gatagaacg cgttttgga 
      ccgagtgaa agaaataaa cagcaaagg gattcccgg 
      ttcaaaatt tgccagtcg atgaattcc tcagggaag 
      ctccaaatt gggggcaaa acctacgat tttacgtcc 
      gatgatctg caggacatt ggggagata ggaaggggc 
      ggttttggg actgtgaat aaaatggtg catagaaag 
      tcgggaact gtggttgcg gtgaagaga ataaggtca 
      acggttgat gaaaaggaa cagaaacaa ctgttgatg 
      gacttggat gtggtgatg aaaagcaac gagtgccat 
      tacatcgtg cagttttat ggggcgctg tttaaggag 
      ggggattgc tggatcgtg atggaacta atggacacc 
      tcattggat aaattctac aaattcatc tacgacaaa 
      ctgggcgat cgaattccg gaagaaatc ctcggaaaa 
      atagcccta gcgactgtg aaagcctta aactactta 
      aaggagaaa ctaaaaata atacatcga gatgtaaaa 
      ccaagcaat attttacta gacaggaag ggaaacatc 
      aagttgtgt gattttgga atttcggga cagttggtc 
      gattcaatt gcacggaca cgtgatgct ggatgtcgc 
      ccgtatatg gctcccgag agaatcgat ccacagacg 
      gccaaaggc tacgacgtg cgaagcgat gtctggtcg 
      ttgggaata acgctgatg gaggtggcc accggtcgt 
      tttccctac ccgagatgg tcgagcgtt ttcgaccaa 
      ttacaccaa gtcgttaac ggggaaccc ccgcgcctt 
      accgaaaac cataacgcc aatactttt acgtcagag 
      tttgttaat tttgtaaat acttgttta ataaaagac 
      gaacactcc agaccaaag tacaagcga ctgctcgaa 
      gacccgttc attttgcgg gcgcagaag gagaaagtg 
      gacgttgca gcctacgtt tcgcgcatc atggacgag 
      atggcgaac aacgggatc agcgcgttc acgacgaac 
      cagcagtga
    • >TC005515 RB
      atggcagat cagtccaat caagtccgg aggcctatg 
      ctgagcata gacttgccc aacagtgtg aattccatc 
      gggggcttt cgcccgctg cccagccag ccgggggcc 
      accagcgag cgtttgggg gccgcagcc gggacaaac 
      cgccccaaa atgagcctc ccgaccatc tccaagtcc 
      acagtcgat cccacgatt gatagaacg cgggattcc 
      cggttcaaa atttgccag tcgatgaat tcctcaggg 
      aagctccaa attgggggc aaaacctac gattttacg 
      tccgatgat ctgcaggac attggggag ataggaagg 
      ggcggtttt gggactgtg aataaaatg gtgcataga 
      aagtcggga actgtggtt gcggtgaag agaataagg 
      tcaacggtt gatgaaaag gaacagaaa caactgttg 
      atggacttg gatgtggtg atgaaaagc aacgagtgc 
      cattacatc gtgcagttt tatggggcg ctgtttaag 
      gagggggat tgctggatc gtgatggaa ctaatggac 
      acctcattg gataaattc tacaaattc atctacgac 
      aaactgggc gatcgaatt ccggaagaa atcctcgga 
      aaaatagcc ctagcgact gtgaaagcc ttaaactac 
      ttaaaggag aaactaaaa ataatacat cgagatgta 
      aaaccaagc aatatttta ctagacagg aagggaaac 
      atcaagttg tgtgatttt ggaatttcg ggacagttg 
      gtcgattca attgcacgg acacgtgat gctggatgt 
      cgcccgtat atggctccc gagagaatc gatccacag 
      acggccaaa ggctacgac gtgcgaagc gatgtctgg 
      tcgttggga ataacgctg atggaggtg gccaccggt 
      cgttttccc tacccgaga tggtcgagc gttttcgac 
      caattacac caagtcgtt aacggggaa cccccgcgc 
      cttaccgaa aaccataac gccaatact tttacgtca 
      gagtttgtt aattttgta aatacttgt ttaataaaa 
      gacgaacac tccagacca aagtacaag cgactgctc 
      gaagacccg ttcattttg cgggcgcag aaggagaaa 
      gtggacgtt gcagcctac gtttcgcgc atcatggac 
      gagatggcg aacaacggg atcagcgcg ttcacgacg 
      aaccagcag tga
    mRNA sequence
    • >TC005515 RA
      cgtcaaaat ggcgtcgtt tagttccat tccaggcag 
      tttttgagt gagttttga gtaaaaatc aaatgttaa 
      tcggtttcg gacaccaga ctaagctta gggacgctt 
      cgatgagct agttcggca cgttagttg tgtttttcg 
      cgtcttaaa tttggtcga atttttgtg caaaatggc 
      agatcagtc caatcaagt ccggaggcc tatgctgag 
      catagactt gcccaacag tgtgaattc catcggggg 
      ctttcgccc gctgcccag ccagccggg ggccaccag 
      cgagcgttt gggggccgc agccgggac aaaccgccc 
      caaaatgag cctcccgac catctccaa gtccacagt 
      cgatcccac gattgatag aacgcgttt tggaccgag 
      tgaaagaaa taaacagca aagggattc ccggttcaa 
      aatttgcca gtcgatgaa ttcctcagg gaagctcca 
      aattggggg caaaaccta cgattttac gtccgatga 
      tctgcagga cattgggga gataggaag gggcggttt 
      tgggactgt gaataaaat ggtgcatag aaagtcggg 
      aactgtggt tgcggtgaa gagaataag gtcaacggt 
      tgatgaaaa ggaacagaa acaactgtt gatggactt 
      ggatgtggt gatgaaaag caacgagtg ccattacat 
      cgtgcagtt ttatggggc gctgtttaa ggaggggga 
      ttgctggat cgtgatgga actaatgga cacctcatt 
      ggataaatt ctacaaatt catctacga caaactggg 
      cgatcgaat tccggaaga aatcctcgg aaaaatagc 
      cctagcgac tgtgaaagc cttaaacta cttaaagga 
      gaaactaaa aataataca tcgagatgt aaaaccaag 
      caatatttt actagacag gaagggaaa catcaagtt 
      gtgtgattt tggaatttc gggacagtt ggtcgattc 
      aattgcacg gacacgtga tgctggatg tcgcccgta 
      tatggctcc cgagagaat cgatccaca gacggccaa 
      aggctacga cgtgcgaag cgatgtctg gtcgttggg 
      aataacgct gatggaggt ggccaccgg tcgttttcc 
      ctacccgag atggtcgag cgttttcga ccaattaca 
      ccaagtcgt taacgggga acccccgcg ccttaccga 
      aaaccataa cgccaatac ttttacgtc agagtttgt 
      taattttgt aaatacttg tttaataaa agacgaaca 
      ctccagacc aaagtacaa gcgactgct cgaagaccc 
      gttcatttt gcgggcgca gaaggagaa agtggacgt 
      tgcagccta cgtttcgcg catcatgga cgagatggc 
      gaacaacgg gatcagcgc gttcacgac gaaccagca 
      gtgaagtaa gcagtcagg tgatgtaga gaaaactgt 
      tagtgagtt gtcgcttaa tcatttatt ggttggtaa 
      gtttgtgca attgcgcgg cgatcgcga ttccttggt 
      gataaactc gagatgaga tttcagacc aatcggatt 
      aaatcgaac taattcgct tgaaaacaa tctgacaat 
      actatcaaa tcgttcggg gttagtccg atttaagtg 
      ttctattga ttggtcgcg ttatgttta ggttcaaat 
      gtaataaat aatagcttt agtaacgta aatgttaca 
      tagatgaag ccactgatt aagaaaaca gtccattgt 
      atataatat agtacatgt ccggtcttg agttcgaat 
      ttaatgtga tgtcttagc atttattgg atgcattcg 
      aggtgcttt tcgcgtata tttttctta ttttcaaat 
      tgttaccga ttttaaaca ataaaacgt tgcctgttc 
      tattgtacc atgtacgat ctttgactg tatataatt 
      agtgtatgt tgttaatat ttaattttt tactcaatt 
      gtttaagaa taagcaaat tgtacgttt atttacgac 
      acgatgttg agtatgtaa agtaaaatt g
    • >TC005515 RB
      cgtcaaaat ggcgtcgtt tagttccat tccaggcag 
      tttttgagt gagttttga gtaaaaatc aaatgttaa 
      tcggtttcg gacaccaga ctaagctta gggacgctt 
      cgatgagct agttcggca cgttagttg tgtttttcg 
      cgtcttaaa tttggtcga atttttgtg caaaatggc 
      agatcagtc caatcaagt ccggaggcc tatgctgag 
      catagactt gcccaacag tgtgaattc catcggggg 
      ctttcgccc gctgcccag ccagccggg ggccaccag 
      cgagcgttt gggggccgc agccgggac aaaccgccc 
      caaaatgag cctcccgac catctccaa gtccacagt 
      cgatcccac gattgatag aacgcggga ttcccggtt 
      caaaatttg ccagtcgat gaattcctc agggaagct 
      ccaaattgg gggcaaaac ctacgattt tacgtccga 
      tgatctgca ggacattgg ggagatagg aaggggcgg 
      ttttgggac tgtgaataa aatggtgca tagaaagtc 
      gggaactgt ggttgcggt gaagagaat aaggtcaac 
      ggttgatga aaaggaaca gaaacaact gttgatgga 
      cttggatgt ggtgatgaa aagcaacga gtgccatta 
      catcgtgca gttttatgg ggcgctgtt taaggaggg 
      ggattgctg gatcgtgat ggaactaat ggacacctc 
      attggataa attctacaa attcatcta cgacaaact 
      gggcgatcg aattccgga agaaatcct cggaaaaat 
      agccctagc gactgtgaa agccttaaa ctacttaaa 
      ggagaaact aaaaataat acatcgaga tgtaaaacc 
      aagcaatat tttactaga caggaaggg aaacatcaa 
      gttgtgtga ttttggaat ttcgggaca gttggtcga 
      ttcaattgc acggacacg tgatgctgg atgtcgccc 
      gtatatggc tcccgagag aatcgatcc acagacggc 
      caaaggcta cgacgtgcg aagcgatgt ctggtcgtt 
      gggaataac gctgatgga ggtggccac cggtcgttt 
      tccctaccc gagatggtc gagcgtttt cgaccaatt 
      acaccaagt cgttaacgg ggaaccccc gcgccttac 
      cgaaaacca taacgccaa tacttttac gtcagagtt 
      tgttaattt tgtaaatac ttgtttaat aaaagacga 
      acactccag accaaagta caagcgact gctcgaaga 
      cccgttcat tttgcgggc gcagaagga gaaagtgga 
      cgttgcagc ctacgtttc gcgcatcat ggacgagat 
      ggcgaacaa cgggatcag cgcgttcac gacgaacca 
      gcagtgaag taagcagtc aggtgatgt agagaaaac 
      tgttagtga gttgtcgct taatcattt attggttgg 
      taagtttgt gcaattgcg cggcgatcg cgattcctt 
      ggtgataaa ctcgagatg agatttcag accaatcgg 
      attaaatcg aactaattc gcttgaaaa caatctgac 
      aatactatc aaatcgttc ggggttagt ccgatttaa 
      gtgttctat tgattggtc gcgttatgt ttaggttca 
      aatgtaata aataatagc tttagtaac gtaaatgtt 
      acatagatg aagccactg attaagaaa acagtccat 
      tgtatataa tatagtaca tgtccggtc ttgagttcg 
      aatttaatg tgatgtctt agcatttat tggatgcat 
      tcgaggtgc ttttcgcgt atatttttc ttattttca 
      aattgttac cgattttaa acaataaaa cgttgcctg 
      ttctattgt accatgtac gatctttga ctgtatata 
      attagtgta tgttgttaa tatttaatt ttttactca 
      attgtttaa gaataagca aattgtacg tttatttac 
      gacacgatg ttgagtatg taaagtaaa attg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY