Status
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Gene information
General information
TC005515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11860686..11863403
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005515
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005515 RA
      MADQSNQVR RPMLSIDLP NSVNSIGGF RPLPSQPGA 
TSERLGAAA GTNRPKMSL PTISKSTVD PTIDRTRFG 
PSERNKQQR DSRFKICQS MNSSGKLQI GGKTYDFTS 
DDLQDIGEI GRGGFGTVN KMVHRKSGT VVAVKRIRS 
TVDEKEQKQ LLMDLDVVM KSNECHYIV QFYGALFKE 
GDCWIVMEL MDTSLDKFY KFIYDKLGD RIPEEILGK 
IALATVKAL NYLKEKLKI IHRDVKPSN ILLDRKGNI 
KLCDFGISG QLVDSIART RDAGCRPYM APERIDPQT 
AKGYDVRSD VWSLGITLM EVATGRFPY PRWSSVFDQ 
LHQVVNGEP PRLTENHNA NTFTSEFVN FVNTCLIKD 
EHSRPKYKR LLEDPFILR AQKEKVDVA AYVSRIMDE 
MANNGISAF TTNQQ
    • >TC005515 RB
      MADQSNQVR RPMLSIDLP NSVNSIGGF RPLPSQPGA 
TSERLGAAA GTNRPKMSL PTISKSTVD PTIDRTRDS 
RFKICQSMN SSGKLQIGG KTYDFTSDD LQDIGEIGR 
GGFGTVNKM VHRKSGTVV AVKRIRSTV DEKEQKQLL 
MDLDVVMKS NECHYIVQF YGALFKEGD CWIVMELMD 
TSLDKFYKF IYDKLGDRI PEEILGKIA LATVKALNY 
LKEKLKIIH RDVKPSNIL LDRKGNIKL CDFGISGQL 
VDSIARTRD AGCRPYMAP ERIDPQTAK GYDVRSDVW 
SLGITLMEV ATGRFPYPR WSSVFDQLH QVVNGEPPR 
LTENHNANT FTSEFVNFV NTCLIKDEH SRPKYKRLL 
EDPFILRAQ KEKVDVAAY VSRIMDEMA NNGISAFTT 
NQQ
    CDS sequence
    • >TC005515 RA
      atggcagat cagtccaat caagtccgg aggcctatg 
ctgagcata gacttgccc aacagtgtg aattccatc 
gggggcttt cgcccgctg cccagccag ccgggggcc 
accagcgag cgtttgggg gccgcagcc gggacaaac 
cgccccaaa atgagcctc ccgaccatc tccaagtcc 
acagtcgat cccacgatt gatagaacg cgttttgga 
ccgagtgaa agaaataaa cagcaaagg gattcccgg 
ttcaaaatt tgccagtcg atgaattcc tcagggaag 
ctccaaatt gggggcaaa acctacgat tttacgtcc 
gatgatctg caggacatt ggggagata ggaaggggc 
ggttttggg actgtgaat aaaatggtg catagaaag 
tcgggaact gtggttgcg gtgaagaga ataaggtca 
acggttgat gaaaaggaa cagaaacaa ctgttgatg 
gacttggat gtggtgatg aaaagcaac gagtgccat 
tacatcgtg cagttttat ggggcgctg tttaaggag 
ggggattgc tggatcgtg atggaacta atggacacc 
tcattggat aaattctac aaattcatc tacgacaaa 
ctgggcgat cgaattccg gaagaaatc ctcggaaaa 
atagcccta gcgactgtg aaagcctta aactactta 
aaggagaaa ctaaaaata atacatcga gatgtaaaa 
ccaagcaat attttacta gacaggaag ggaaacatc 
aagttgtgt gattttgga atttcggga cagttggtc 
gattcaatt gcacggaca cgtgatgct ggatgtcgc 
ccgtatatg gctcccgag agaatcgat ccacagacg 
gccaaaggc tacgacgtg cgaagcgat gtctggtcg 
ttgggaata acgctgatg gaggtggcc accggtcgt 
tttccctac ccgagatgg tcgagcgtt ttcgaccaa 
ttacaccaa gtcgttaac ggggaaccc ccgcgcctt 
accgaaaac cataacgcc aatactttt acgtcagag 
tttgttaat tttgtaaat acttgttta ataaaagac 
gaacactcc agaccaaag tacaagcga ctgctcgaa 
gacccgttc attttgcgg gcgcagaag gagaaagtg 
gacgttgca gcctacgtt tcgcgcatc atggacgag 
atggcgaac aacgggatc agcgcgttc acgacgaac 
cagcagtga
    • >TC005515 RB
      atggcagat cagtccaat caagtccgg aggcctatg 
ctgagcata gacttgccc aacagtgtg aattccatc 
gggggcttt cgcccgctg cccagccag ccgggggcc 
accagcgag cgtttgggg gccgcagcc gggacaaac 
cgccccaaa atgagcctc ccgaccatc tccaagtcc 
acagtcgat cccacgatt gatagaacg cgggattcc 
cggttcaaa atttgccag tcgatgaat tcctcaggg 
aagctccaa attgggggc aaaacctac gattttacg 
tccgatgat ctgcaggac attggggag ataggaagg 
ggcggtttt gggactgtg aataaaatg gtgcataga 
aagtcggga actgtggtt gcggtgaag agaataagg 
tcaacggtt gatgaaaag gaacagaaa caactgttg 
atggacttg gatgtggtg atgaaaagc aacgagtgc 
cattacatc gtgcagttt tatggggcg ctgtttaag 
gagggggat tgctggatc gtgatggaa ctaatggac 
acctcattg gataaattc tacaaattc atctacgac 
aaactgggc gatcgaatt ccggaagaa atcctcgga 
aaaatagcc ctagcgact gtgaaagcc ttaaactac 
ttaaaggag aaactaaaa ataatacat cgagatgta 
aaaccaagc aatatttta ctagacagg aagggaaac 
atcaagttg tgtgatttt ggaatttcg ggacagttg 
gtcgattca attgcacgg acacgtgat gctggatgt 
cgcccgtat atggctccc gagagaatc gatccacag 
acggccaaa ggctacgac gtgcgaagc gatgtctgg 
tcgttggga ataacgctg atggaggtg gccaccggt 
cgttttccc tacccgaga tggtcgagc gttttcgac 
caattacac caagtcgtt aacggggaa cccccgcgc 
cttaccgaa aaccataac gccaatact tttacgtca 
gagtttgtt aattttgta aatacttgt ttaataaaa 
gacgaacac tccagacca aagtacaag cgactgctc 
gaagacccg ttcattttg cgggcgcag aaggagaaa 
gtggacgtt gcagcctac gtttcgcgc atcatggac 
gagatggcg aacaacggg atcagcgcg ttcacgacg 
aaccagcag tga
    mRNA sequence
    • >TC005515 RA
      cgtcaaaat ggcgtcgtt tagttccat tccaggcag 
tttttgagt gagttttga gtaaaaatc aaatgttaa 
tcggtttcg gacaccaga ctaagctta gggacgctt 
cgatgagct agttcggca cgttagttg tgtttttcg 
cgtcttaaa tttggtcga atttttgtg caaaatggc 
agatcagtc caatcaagt ccggaggcc tatgctgag 
catagactt gcccaacag tgtgaattc catcggggg 
ctttcgccc gctgcccag ccagccggg ggccaccag 
cgagcgttt gggggccgc agccgggac aaaccgccc 
caaaatgag cctcccgac catctccaa gtccacagt 
cgatcccac gattgatag aacgcgttt tggaccgag 
tgaaagaaa taaacagca aagggattc ccggttcaa 
aatttgcca gtcgatgaa ttcctcagg gaagctcca 
aattggggg caaaaccta cgattttac gtccgatga 
tctgcagga cattgggga gataggaag gggcggttt 
tgggactgt gaataaaat ggtgcatag aaagtcggg 
aactgtggt tgcggtgaa gagaataag gtcaacggt 
tgatgaaaa ggaacagaa acaactgtt gatggactt 
ggatgtggt gatgaaaag caacgagtg ccattacat 
cgtgcagtt ttatggggc gctgtttaa ggaggggga 
ttgctggat cgtgatgga actaatgga cacctcatt 
ggataaatt ctacaaatt catctacga caaactggg 
cgatcgaat tccggaaga aatcctcgg aaaaatagc 
cctagcgac tgtgaaagc cttaaacta cttaaagga 
gaaactaaa aataataca tcgagatgt aaaaccaag 
caatatttt actagacag gaagggaaa catcaagtt 
gtgtgattt tggaatttc gggacagtt ggtcgattc 
aattgcacg gacacgtga tgctggatg tcgcccgta 
tatggctcc cgagagaat cgatccaca gacggccaa 
aggctacga cgtgcgaag cgatgtctg gtcgttggg 
aataacgct gatggaggt ggccaccgg tcgttttcc 
ctacccgag atggtcgag cgttttcga ccaattaca 
ccaagtcgt taacgggga acccccgcg ccttaccga 
aaaccataa cgccaatac ttttacgtc agagtttgt 
taattttgt aaatacttg tttaataaa agacgaaca 
ctccagacc aaagtacaa gcgactgct cgaagaccc 
gttcatttt gcgggcgca gaaggagaa agtggacgt 
tgcagccta cgtttcgcg catcatgga cgagatggc 
gaacaacgg gatcagcgc gttcacgac gaaccagca 
gtgaagtaa gcagtcagg tgatgtaga gaaaactgt 
tagtgagtt gtcgcttaa tcatttatt ggttggtaa 
gtttgtgca attgcgcgg cgatcgcga ttccttggt 
gataaactc gagatgaga tttcagacc aatcggatt 
aaatcgaac taattcgct tgaaaacaa tctgacaat 
actatcaaa tcgttcggg gttagtccg atttaagtg 
ttctattga ttggtcgcg ttatgttta ggttcaaat 
gtaataaat aatagcttt agtaacgta aatgttaca 
tagatgaag ccactgatt aagaaaaca gtccattgt 
atataatat agtacatgt ccggtcttg agttcgaat 
ttaatgtga tgtcttagc atttattgg atgcattcg 
aggtgcttt tcgcgtata tttttctta ttttcaaat 
tgttaccga ttttaaaca ataaaacgt tgcctgttc 
tattgtacc atgtacgat ctttgactg tatataatt 
agtgtatgt tgttaatat ttaattttt tactcaatt 
gtttaagaa taagcaaat tgtacgttt atttacgac 
acgatgttg agtatgtaa agtaaaatt g
    • >TC005515 RB
      cgtcaaaat ggcgtcgtt tagttccat tccaggcag 
tttttgagt gagttttga gtaaaaatc aaatgttaa 
tcggtttcg gacaccaga ctaagctta gggacgctt 
cgatgagct agttcggca cgttagttg tgtttttcg 
cgtcttaaa tttggtcga atttttgtg caaaatggc 
agatcagtc caatcaagt ccggaggcc tatgctgag 
catagactt gcccaacag tgtgaattc catcggggg 
ctttcgccc gctgcccag ccagccggg ggccaccag 
cgagcgttt gggggccgc agccgggac aaaccgccc 
caaaatgag cctcccgac catctccaa gtccacagt 
cgatcccac gattgatag aacgcggga ttcccggtt 
caaaatttg ccagtcgat gaattcctc agggaagct 
ccaaattgg gggcaaaac ctacgattt tacgtccga 
tgatctgca ggacattgg ggagatagg aaggggcgg 
ttttgggac tgtgaataa aatggtgca tagaaagtc 
gggaactgt ggttgcggt gaagagaat aaggtcaac 
ggttgatga aaaggaaca gaaacaact gttgatgga 
cttggatgt ggtgatgaa aagcaacga gtgccatta 
catcgtgca gttttatgg ggcgctgtt taaggaggg 
ggattgctg gatcgtgat ggaactaat ggacacctc 
attggataa attctacaa attcatcta cgacaaact 
gggcgatcg aattccgga agaaatcct cggaaaaat 
agccctagc gactgtgaa agccttaaa ctacttaaa 
ggagaaact aaaaataat acatcgaga tgtaaaacc 
aagcaatat tttactaga caggaaggg aaacatcaa 
gttgtgtga ttttggaat ttcgggaca gttggtcga 
ttcaattgc acggacacg tgatgctgg atgtcgccc 
gtatatggc tcccgagag aatcgatcc acagacggc 
caaaggcta cgacgtgcg aagcgatgt ctggtcgtt 
gggaataac gctgatgga ggtggccac cggtcgttt 
tccctaccc gagatggtc gagcgtttt cgaccaatt 
acaccaagt cgttaacgg ggaaccccc gcgccttac 
cgaaaacca taacgccaa tacttttac gtcagagtt 
tgttaattt tgtaaatac ttgtttaat aaaagacga 
acactccag accaaagta caagcgact gctcgaaga 
cccgttcat tttgcgggc gcagaagga gaaagtgga 
cgttgcagc ctacgtttc gcgcatcat ggacgagat 
ggcgaacaa cgggatcag cgcgttcac gacgaacca 
gcagtgaag taagcagtc aggtgatgt agagaaaac 
tgttagtga gttgtcgct taatcattt attggttgg 
taagtttgt gcaattgcg cggcgatcg cgattcctt 
ggtgataaa ctcgagatg agatttcag accaatcgg 
attaaatcg aactaattc gcttgaaaa caatctgac 
aatactatc aaatcgttc ggggttagt ccgatttaa 
gtgttctat tgattggtc gcgttatgt ttaggttca 
aatgtaata aataatagc tttagtaac gtaaatgtt 
acatagatg aagccactg attaagaaa acagtccat 
tgtatataa tatagtaca tgtccggtc ttgagttcg 
aatttaatg tgatgtctt agcatttat tggatgcat 
tcgaggtgc ttttcgcgt atatttttc ttattttca 
aattgttac cgattttaa acaataaaa cgttgcctg 
ttctattgt accatgtac gatctttga ctgtatata 
attagtgta tgttgttaa tatttaatt ttttactca 
attgtttaa gaataagca aattgtacg tttatttac 
gacacgatg ttgagtatg taaagtaaa attg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY