| General information | |||
| TC005515 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 11860686..11863403 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005515 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00872 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 3
| Comment: |
- >TC005515 RA
MADQSNQVR RPMLSIDLP NSVNSIGGF RPLPSQPGA TSERLGAAA GTNRPKMSL PTISKSTVD PTIDRTRFG PSERNKQQR DSRFKICQS MNSSGKLQI GGKTYDFTS DDLQDIGEI GRGGFGTVN KMVHRKSGT VVAVKRIRS TVDEKEQKQ LLMDLDVVM KSNECHYIV QFYGALFKE GDCWIVMEL MDTSLDKFY KFIYDKLGD RIPEEILGK IALATVKAL NYLKEKLKI IHRDVKPSN ILLDRKGNI KLCDFGISG QLVDSIART RDAGCRPYM APERIDPQT AKGYDVRSD VWSLGITLM EVATGRFPY PRWSSVFDQ LHQVVNGEP PRLTENHNA NTFTSEFVN FVNTCLIKD EHSRPKYKR LLEDPFILR AQKEKVDVA AYVSRIMDE MANNGISAF TTNQQ
- >TC005515 RB
MADQSNQVR RPMLSIDLP NSVNSIGGF RPLPSQPGA TSERLGAAA GTNRPKMSL PTISKSTVD PTIDRTRDS RFKICQSMN SSGKLQIGG KTYDFTSDD LQDIGEIGR GGFGTVNKM VHRKSGTVV AVKRIRSTV DEKEQKQLL MDLDVVMKS NECHYIVQF YGALFKEGD CWIVMELMD TSLDKFYKF IYDKLGDRI PEEILGKIA LATVKALNY LKEKLKIIH RDVKPSNIL LDRKGNIKL CDFGISGQL VDSIARTRD AGCRPYMAP ERIDPQTAK GYDVRSDVW SLGITLMEV ATGRFPYPR WSSVFDQLH QVVNGEPPR LTENHNANT FTSEFVNFV NTCLIKDEH SRPKYKRLL EDPFILRAQ KEKVDVAAY VSRIMDEMA NNGISAFTT NQQ
- >TC005515 RA
atggcagat cagtccaat caagtccgg aggcctatg ctgagcata gacttgccc aacagtgtg aattccatc gggggcttt cgcccgctg cccagccag ccgggggcc accagcgag cgtttgggg gccgcagcc gggacaaac cgccccaaa atgagcctc ccgaccatc tccaagtcc acagtcgat cccacgatt gatagaacg cgttttgga ccgagtgaa agaaataaa cagcaaagg gattcccgg ttcaaaatt tgccagtcg atgaattcc tcagggaag ctccaaatt gggggcaaa acctacgat tttacgtcc gatgatctg caggacatt ggggagata ggaaggggc ggttttggg actgtgaat aaaatggtg catagaaag tcgggaact gtggttgcg gtgaagaga ataaggtca acggttgat gaaaaggaa cagaaacaa ctgttgatg gacttggat gtggtgatg aaaagcaac gagtgccat tacatcgtg cagttttat ggggcgctg tttaaggag ggggattgc tggatcgtg atggaacta atggacacc tcattggat aaattctac aaattcatc tacgacaaa ctgggcgat cgaattccg gaagaaatc ctcggaaaa atagcccta gcgactgtg aaagcctta aactactta aaggagaaa ctaaaaata atacatcga gatgtaaaa ccaagcaat attttacta gacaggaag ggaaacatc aagttgtgt gattttgga atttcggga cagttggtc gattcaatt gcacggaca cgtgatgct ggatgtcgc ccgtatatg gctcccgag agaatcgat ccacagacg gccaaaggc tacgacgtg cgaagcgat gtctggtcg ttgggaata acgctgatg gaggtggcc accggtcgt tttccctac ccgagatgg tcgagcgtt ttcgaccaa ttacaccaa gtcgttaac ggggaaccc ccgcgcctt accgaaaac cataacgcc aatactttt acgtcagag tttgttaat tttgtaaat acttgttta ataaaagac gaacactcc agaccaaag tacaagcga ctgctcgaa gacccgttc attttgcgg gcgcagaag gagaaagtg gacgttgca gcctacgtt tcgcgcatc atggacgag atggcgaac aacgggatc agcgcgttc acgacgaac cagcagtga
- >TC005515 RB
atggcagat cagtccaat caagtccgg aggcctatg ctgagcata gacttgccc aacagtgtg aattccatc gggggcttt cgcccgctg cccagccag ccgggggcc accagcgag cgtttgggg gccgcagcc gggacaaac cgccccaaa atgagcctc ccgaccatc tccaagtcc acagtcgat cccacgatt gatagaacg cgggattcc cggttcaaa atttgccag tcgatgaat tcctcaggg aagctccaa attgggggc aaaacctac gattttacg tccgatgat ctgcaggac attggggag ataggaagg ggcggtttt gggactgtg aataaaatg gtgcataga aagtcggga actgtggtt gcggtgaag agaataagg tcaacggtt gatgaaaag gaacagaaa caactgttg atggacttg gatgtggtg atgaaaagc aacgagtgc cattacatc gtgcagttt tatggggcg ctgtttaag gagggggat tgctggatc gtgatggaa ctaatggac acctcattg gataaattc tacaaattc atctacgac aaactgggc gatcgaatt ccggaagaa atcctcgga aaaatagcc ctagcgact gtgaaagcc ttaaactac ttaaaggag aaactaaaa ataatacat cgagatgta aaaccaagc aatatttta ctagacagg aagggaaac atcaagttg tgtgatttt ggaatttcg ggacagttg gtcgattca attgcacgg acacgtgat gctggatgt cgcccgtat atggctccc gagagaatc gatccacag acggccaaa ggctacgac gtgcgaagc gatgtctgg tcgttggga ataacgctg atggaggtg gccaccggt cgttttccc tacccgaga tggtcgagc gttttcgac caattacac caagtcgtt aacggggaa cccccgcgc cttaccgaa aaccataac gccaatact tttacgtca gagtttgtt aattttgta aatacttgt ttaataaaa gacgaacac tccagacca aagtacaag cgactgctc gaagacccg ttcattttg cgggcgcag aaggagaaa gtggacgtt gcagcctac gtttcgcgc atcatggac gagatggcg aacaacggg atcagcgcg ttcacgacg aaccagcag tga
- >TC005515 RA
cgtcaaaat ggcgtcgtt tagttccat tccaggcag tttttgagt gagttttga gtaaaaatc aaatgttaa tcggtttcg gacaccaga ctaagctta gggacgctt cgatgagct agttcggca cgttagttg tgtttttcg cgtcttaaa tttggtcga atttttgtg caaaatggc agatcagtc caatcaagt ccggaggcc tatgctgag catagactt gcccaacag tgtgaattc catcggggg ctttcgccc gctgcccag ccagccggg ggccaccag cgagcgttt gggggccgc agccgggac aaaccgccc caaaatgag cctcccgac catctccaa gtccacagt cgatcccac gattgatag aacgcgttt tggaccgag tgaaagaaa taaacagca aagggattc ccggttcaa aatttgcca gtcgatgaa ttcctcagg gaagctcca aattggggg caaaaccta cgattttac gtccgatga tctgcagga cattgggga gataggaag gggcggttt tgggactgt gaataaaat ggtgcatag aaagtcggg aactgtggt tgcggtgaa gagaataag gtcaacggt tgatgaaaa ggaacagaa acaactgtt gatggactt ggatgtggt gatgaaaag caacgagtg ccattacat cgtgcagtt ttatggggc gctgtttaa ggaggggga ttgctggat cgtgatgga actaatgga cacctcatt ggataaatt ctacaaatt catctacga caaactggg cgatcgaat tccggaaga aatcctcgg aaaaatagc cctagcgac tgtgaaagc cttaaacta cttaaagga gaaactaaa aataataca tcgagatgt aaaaccaag caatatttt actagacag gaagggaaa catcaagtt gtgtgattt tggaatttc gggacagtt ggtcgattc aattgcacg gacacgtga tgctggatg tcgcccgta tatggctcc cgagagaat cgatccaca gacggccaa aggctacga cgtgcgaag cgatgtctg gtcgttggg aataacgct gatggaggt ggccaccgg tcgttttcc ctacccgag atggtcgag cgttttcga ccaattaca ccaagtcgt taacgggga acccccgcg ccttaccga aaaccataa cgccaatac ttttacgtc agagtttgt taattttgt aaatacttg tttaataaa agacgaaca ctccagacc aaagtacaa gcgactgct cgaagaccc gttcatttt gcgggcgca gaaggagaa agtggacgt tgcagccta cgtttcgcg catcatgga cgagatggc gaacaacgg gatcagcgc gttcacgac gaaccagca gtgaagtaa gcagtcagg tgatgtaga gaaaactgt tagtgagtt gtcgcttaa tcatttatt ggttggtaa gtttgtgca attgcgcgg cgatcgcga ttccttggt gataaactc gagatgaga tttcagacc aatcggatt aaatcgaac taattcgct tgaaaacaa tctgacaat actatcaaa tcgttcggg gttagtccg atttaagtg ttctattga ttggtcgcg ttatgttta ggttcaaat gtaataaat aatagcttt agtaacgta aatgttaca tagatgaag ccactgatt aagaaaaca gtccattgt atataatat agtacatgt ccggtcttg agttcgaat ttaatgtga tgtcttagc atttattgg atgcattcg aggtgcttt tcgcgtata tttttctta ttttcaaat tgttaccga ttttaaaca ataaaacgt tgcctgttc tattgtacc atgtacgat ctttgactg tatataatt agtgtatgt tgttaatat ttaattttt tactcaatt gtttaagaa taagcaaat tgtacgttt atttacgac acgatgttg agtatgtaa agtaaaatt g
- >TC005515 RB
cgtcaaaat ggcgtcgtt tagttccat tccaggcag tttttgagt gagttttga gtaaaaatc aaatgttaa tcggtttcg gacaccaga ctaagctta gggacgctt cgatgagct agttcggca cgttagttg tgtttttcg cgtcttaaa tttggtcga atttttgtg caaaatggc agatcagtc caatcaagt ccggaggcc tatgctgag catagactt gcccaacag tgtgaattc catcggggg ctttcgccc gctgcccag ccagccggg ggccaccag cgagcgttt gggggccgc agccgggac aaaccgccc caaaatgag cctcccgac catctccaa gtccacagt cgatcccac gattgatag aacgcggga ttcccggtt caaaatttg ccagtcgat gaattcctc agggaagct ccaaattgg gggcaaaac ctacgattt tacgtccga tgatctgca ggacattgg ggagatagg aaggggcgg ttttgggac tgtgaataa aatggtgca tagaaagtc gggaactgt ggttgcggt gaagagaat aaggtcaac ggttgatga aaaggaaca gaaacaact gttgatgga cttggatgt ggtgatgaa aagcaacga gtgccatta catcgtgca gttttatgg ggcgctgtt taaggaggg ggattgctg gatcgtgat ggaactaat ggacacctc attggataa attctacaa attcatcta cgacaaact gggcgatcg aattccgga agaaatcct cggaaaaat agccctagc gactgtgaa agccttaaa ctacttaaa ggagaaact aaaaataat acatcgaga tgtaaaacc aagcaatat tttactaga caggaaggg aaacatcaa gttgtgtga ttttggaat ttcgggaca gttggtcga ttcaattgc acggacacg tgatgctgg atgtcgccc gtatatggc tcccgagag aatcgatcc acagacggc caaaggcta cgacgtgcg aagcgatgt ctggtcgtt gggaataac gctgatgga ggtggccac cggtcgttt tccctaccc gagatggtc gagcgtttt cgaccaatt acaccaagt cgttaacgg ggaaccccc gcgccttac cgaaaacca taacgccaa tacttttac gtcagagtt tgttaattt tgtaaatac ttgtttaat aaaagacga acactccag accaaagta caagcgact gctcgaaga cccgttcat tttgcgggc gcagaagga gaaagtgga cgttgcagc ctacgtttc gcgcatcat ggacgagat ggcgaacaa cgggatcag cgcgttcac gacgaacca gcagtgaag taagcagtc aggtgatgt agagaaaac tgttagtga gttgtcgct taatcattt attggttgg taagtttgt gcaattgcg cggcgatcg cgattcctt ggtgataaa ctcgagatg agatttcag accaatcgg attaaatcg aactaattc gcttgaaaa caatctgac aatactatc aaatcgttc ggggttagt ccgatttaa gtgttctat tgattggtc gcgttatgt ttaggttca aatgtaata aataatagc tttagtaac gtaaatgtt acatagatg aagccactg attaagaaa acagtccat tgtatataa tatagtaca tgtccggtc ttgagttcg aatttaatg tgatgtctt agcatttat tggatgcat tcgaggtgc ttttcgcgt atatttttc ttattttca aattgttac cgattttaa acaataaaa cgttgcctg ttctattgt accatgtac gatctttga ctgtatata attagtgta tgttgttaa tatttaatt ttttactca attgtttaa gaataagca aattgtacg tttatttac gacacgatg ttgagtatg taaagtaaa attg
