Status
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Gene information
General information
TC031287
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005509
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11919598..11940134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031287
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00871

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031287 RA
      MPGSHRSSE LTWSSTSRI SYSEHKYAK CFVIPQRYL 
      FCLLASLIM LNAYSIHSA LEVIAVEYF DSDKTTNTS 
      DTCPSTPDT RKQIAKRPR NRSLLVYDW SLETRLIIK 
      DSFYWSRLL TIIPGGFVS DQLGGKYVV TLSVLFSAF 
      CHLMAPAVV DLGAGKVGF VVALRVLNG CFQGVLQSA 
      VLSLLARWA PPSERALMA SLVYGSASL GEQIAIAMH 
      KAFMNYTWR PMFYLSAGV HVVFCFVWH LLVYPSHRD 
      NPFLTVREK RFLDEEADE SLVEGWKKV PWGRILKSG 
      ALWSLGFLQ VGSSWSYQV YRKILEWYL PRVLKSGVP 
      WESAWWTPF FLYFWLTIF CGFVADWFV KGNHLSVTT 
      VRKTFALIG NYLTALCLM FVTFEGCNS VTVMVYTTV 
      WLCLDATYY ASTLVLPLD LTSNFAGIT IGIMWTVEN 
      LVIGLSRYV VNLLTTDSR MNSNVPYVV LFTIQIIGI 
      SNQQLIDLP SEMKEVLLE ISPNFDENL KRAFRNEGV 
      RLQRVQNNG RFIHQLDDV LLSIDDTKI ELRNLKFPW 
      IPDFRIVDL SSDLPMSCL DLNLNLGNL RIEGEYEAN 
      NTTLRRWLP VSHIGRIVI GFNNVRANG KVGLVLEQD 
      SFVPQNYDI RYEPTDVVI RVSYHVDGE NEVQNEISN 
      SDIEATLGK TVWVQLTEI LSNLLHRQL GEVVVEFSV 
      TELLVDRDE EYREYAKGQ AARANKLLD SLLCSAKDY 
      LVAKDLRTV KTPPFDVVF KGKVSGVQQ GTFSTGEGF 
      LQDLATLTR RHSFSLYED KHKLTIYGG IRLREFKLG 
      YGGYQGQYE ETAVSGSIK GSLYKNEIF VKITVKKEG 
      ERCSTQLDS VQVVVVK
    CDS sequence
    • >TC031287 RA
      atgcctggt tcgcaccga agtagcgaa ctgacttgg 
      agttccacc tcccgtatt tcctactcc gaacacaaa 
      tacgcaaaa tgtttcgtg atcccccaa cgctactta 
      ttctgcctc ctcgcctcc ctcataatg ctcaacgct 
      tactcgatc cactccgcc ctcgaggtc atcgcagtg 
      gaatacttc gactcggac aaaaccacc aacaccagc 
      gacacgtgc cctagcacc cccgacacc aggaaacaa 
      atcgccaag cggccgcga aaccgctcg ctgctggtc 
      tacgactgg agtctcgag acccggctc attatcaaa 
      gactcgttc tactggagc cgcctgttg actattatc 
      cccggcggt ttcgtctcg gaccagttg gggggcaag 
      tacgtcgtc accctttct gtgcttttc tcggcgttc 
      tgccacctg atggcgccc gcggtcgtc gacctgggt 
      gcgggcaag gtggggttt gtggtcgcg ctccgggtg 
      ctgaacggg tgcttccaa ggggttttg cagtcggcg 
      gttctgtcc ctgctggcc cgttgggcg ccgccgagc 
      gaacgcgcc ctgatggcg agtctggtc tacgggagc 
      gcgtctttg ggggagcag atcgccatc gccatgcac 
      aaggccttc atgaactac acctggagg ccgatgttc 
      tacttgtcg gctggggtg cacgtcgtt ttttgcttc 
      gtctggcac ttgctggtc tacccgtcc cacagagac 
      aaccccttc ttgactgtg cgcgaaaag cggtttttg 
      gacgaagag gcggacgag agtctggtt gaggggtgg 
      aagaaagtg ccatggggg aggattctc aagtcgggc 
      gcgttgtgg tcgctgggg ttcctccaa gtgggctcg 
      agctggtcc taccaggtg tataggaaa atcctggag 
      tggtatttg ccgagggtt ttgaagtcg ggagtgccg 
      tgggagtcg gcttggtgg acgccgttt ttcctctat 
      ttctggctg acgattttt tgtggtttt gtggccgat 
      tggtttgtg aaaggaaat catctaagt gtgacaact 
      gttcggaaa actttcgct ctgattggc aactacctt 
      acggctctg tgtctcatg tttgtgact tttgagggt 
      tgtaatagt gtcacagtt atggtttac accacagtt 
      tggttgtgt ctagacgcc acctattat gcctcaacg 
      cttgtgctt ccgttagat ttgacctca aattttgcc 
      gggattaca atagggata atgtggacg gttgaaaat 
      ctcgttata ggtttatcg agatatgtt gtgaattta 
      cttaccaca gattccaga atgaattcc aacgtccca 
      tatgttgtt ttatttaca attcaaata attggaata 
      tcgaatcag caactgata gatctgcct agcgaaatg 
      aaagaagtt ttactagaa attagcccg aactttgat 
      gaaaatctg aaacgggct ttcaggaac gaaggagtg 
      aggctgcag agagtgcag aacaatgga cgatttatt 
      catcagctg gacgacgtt ctcttatcc atagacgat 
      accaaaatc gagttacgc aacctgaaa ttcccctgg 
      atccccgac ttccgcatc gtggacttg tccagcgac 
      ctgcccatg tcatgcctc gacctaaac ctaaacctg 
      ggcaatttg cgaattgag ggcgagtac gaagccaac 
      aacaccaca ctcaggcga tggctcccg gtatctcac 
      attggtcga atcgtgatc ggttttaac aacgtccga 
      gcgaacgga aaagtcgga ctcgtgctc gagcaggat 
      tctttcgtt ccgcagaat tatgatatt agatatgag 
      ccgacggat gttgttatt agggttagc tatcacgtg 
      gatggcgag aatgaggtg caaaatgag attagcaat 
      tcagatatt gaggccacg ctaggcaag accgtgtgg 
      gtgcagttg actgagata ttgtccaac ctgttgcat 
      aggcaattg ggcgaggtt gtagtggag ttttccgtg 
      acggaactc ctcgtcgat agggacgag gaatacagg 
      gaatacgcc aagggacaa gcagcgcgc gccaataaa 
      ctcctggac tcgcttttg tgctcagcc aaggactat 
      ttagtcgcg aaggacttg aggacggtc aaaacgcca 
      cccttcgac gtcgtcttc aaagggaaa gtctcgggg 
      gtgcagcag gggaccttc agcacgggg gaagggttc 
      ctccaagac ctcgccact ttgacgcgg agacacagc 
      tttagtttg tacgaagac aaacacaaa ctcacgata 
      tatgggggg atcaggctg agggagttt aaactcggg 
      tatgggggc taccagggc cagtacgag gaaacggcc 
      gtctcaggc agcatcaaa ggctcgctc tacaagaac 
      gagattttc gtcaagatc actgtgaaa aaagaaggc 
      gagcggtgc tcgactcag ttagattcc gtccaagtt 
      gttgttgta aagtaa
    mRNA sequence
    • >TC031287 RA
      taactcttt tggaacctt ctatcaaat ccacactcg 
      aaatgtcaa atcgctgcc aaatctctc aaaattttt 
      cacgttttt cgcgccatg cctggttcg caccgaagt 
      agcgaactg acttggagt tccacctcc cgtatttcc 
      tactccgaa cacaaatac gcaaaatgt ttcgtgatc 
      ccccaacgc tacttattc tgcctcctc gcctccctc 
      ataatgctc aacgcttac tcgatccac tccgccctc 
      gaggtcatc gcagtggaa tacttcgac tcggacaaa 
      accaccaac accagcgac acgtgccct agcaccccc 
      gacaccagg aaacaaatc gccaagcgg ccgcgaaac 
      cgctcgctg ctggtctac gactggagt ctcgagacc 
      cggctcatt atcaaagac tcgttctac tggagccgc 
      ctgttgact attatcccc ggcggtttc gtctcggac 
      cagttgggg ggcaagtac gtcgtcacc ctttctgtg 
      cttttctcg gcgttctgc cacctgatg gcgcccgcg 
      gtcgtcgac ctgggtgcg ggcaaggtg gggtttgtg 
      gtcgcgctc cgggtgctg aacgggtgc ttccaaggg 
      gttttgcag tcggcggtt ctgtccctg ctggcccgt 
      tgggcgccg ccgagcgaa cgcgccctg atggcgagt 
      ctggtctac gggagcgcg tctttgggg gagcagatc 
      gccatcgcc atgcacaag gccttcatg aactacacc 
      tggaggccg atgttctac ttgtcggct ggggtgcac 
      gtcgttttt tgcttcgtc tggcacttg ctggtctac 
      ccgtcccac agagacaac cccttcttg actgtgcgc 
      gaaaagcgg tttttggac gaagaggcg gacgagagt 
      ctggttgag gggtggaag aaagtgcca tgggggagg 
      attctcaag tcgggcgcg ttgtggtcg ctggggttc 
      ctccaagtg ggctcgagc tggtcctac caggtgtat 
      aggaaaatc ctggagtgg tatttgccg agggttttg 
      aagtcggga gtgccgtgg gagtcggct tggtggacg 
      ccgtttttc ctctatttc tggctgacg attttttgt 
      ggttttgtg gccgattgg tttgtgaaa ggaaatcat 
      ctaagtgtg acaactgtt cggaaaact ttcgctctg 
      attggcaac taccttacg gctctgtgt ctcatgttt 
      gtgactttt gagggttgt aatagtgtc acagttatg 
      gtttacacc acagtttgg ttgtgtcta gacgccacc 
      tattatgcc tcaacgctt gtgcttccg ttagatttg 
      acctcaaat tttgccggg attacaata gggataatg 
      tggacggtt gaaaatctc gttataggt ttatcgaga 
      tatgttgtg aatttactt accacagat tccagaatg 
      aattccaac gtcccatat gttgtttta tttacaatt 
      caaataatt ggaatatcg aatcagcaa ctgatagat 
      ctgcctagc gaaatgaaa gaagtttta ctagaaatt 
      agcccgaac tttgatgaa aatctgaaa cgggctttc 
      aggaacgaa ggagtgagg ctgcagaga gtgcagaac 
      aatggacga tttattcat cagctggac gacgttctc 
      ttatccata gacgatacc aaaatcgag ttacgcaac 
      ctgaaattc ccctggatc cccgacttc cgcatcgtg 
      gacttgtcc agcgacctg cccatgtca tgcctcgac 
      ctaaaccta aacctgggc aatttgcga attgagggc 
      gagtacgaa gccaacaac accacactc aggcgatgg 
      ctcccggta tctcacatt ggtcgaatc gtgatcggt 
      tttaacaac gtccgagcg aacggaaaa gtcggactc 
      gtgctcgag caggattct ttcgttccg cagaattat 
      gatattaga tatgagccg acggatgtt gttattagg 
      gttagctat cacgtggat ggcgagaat gaggtgcaa 
      aatgagatt agcaattca gatattgag gccacgcta 
      ggcaagacc gtgtgggtg cagttgact gagatattg 
      tccaacctg ttgcatagg caattgggc gaggttgta 
      gtggagttt tccgtgacg gaactcctc gtcgatagg 
      gacgaggaa tacagggaa tacgccaag ggacaagca 
      gcgcgcgcc aataaactc ctggactcg cttttgtgc 
      tcagccaag gactattta gtcgcgaag gacttgagg 
      acggtcaaa acgccaccc ttcgacgtc gtcttcaaa 
      gggaaagtc tcgggggtg cagcagggg accttcagc 
      acgggggaa gggttcctc caagacctc gccactttg 
      acgcggaga cacagcttt agtttgtac gaagacaaa 
      cacaaactc acgatatat ggggggatc aggctgagg 
      gagtttaaa ctcgggtat gggggctac cagggccag 
      tacgaggaa acggccgtc tcaggcagc atcaaaggc 
      tcgctctac aagaacgag attttcgtc aagatcact 
      gtgaaaaaa gaaggcgag cggtgctcg actcagtta 
      gattccgtc caagttgtt gttgtaaag taagtctat 
      tttcactga aaacatctc cattttcct aattatgcg 
      cctaatttg tggcccgga atcgggtca attttcccg 
      agataacaa acgagattg aaacagacg cattgttgg 
      ttttccatt aaagtcggc ggaaaaatt tatcgctcg 
      agaaagttt caaacgcta gtttaataa aagtcgggt 
      gcgcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY