| General information | |||
| TC005496 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12104967..12111709 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005496 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00869 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC005496 RA
MKMVLSQRQ REELNKAIA DYLGSNGYM NALEAFKKE TDMPGELER KFGGLLEKK WTSVIRLQK KVMELESKL HEAEKEYIE GAPTRGKRS PAEWIPRPP EKYALTGHR APVTRVIFH PLFSLFVST SEDATIKVW DFETGEYER TLKGHSDAV QDVAFDATG KLLVSCSAD MSIKLWDFQ QSYECIRTM LGHDHNISS VAFMPAGDF VVSSSRDKT IKMWEVSTG YCVKTFTGH KDWVRVVRP SPDGQLLAS CSNDQTIRI WQVYTKECR AELRAHEHV VEFVAWAPD SAANAINEA AGVDNKKGA HMGPFLASC SRDKTIRIW DVGAGVALF VLIGHDNWV RGAVFHPGG KFLVSVSDD KTLRVWDLR NKRCLKTLD AHKHFCTSV DFHKSHPYV ISGSVDLTV KVWECR
- >TC005496 RB
MVLSQRQRE ELNKAIADY LGSNGYMNA LEAFKKETD MPGELERKF GGLLEKKWT SVIRLQKKV MELESKLHE AEKEYIEGA PTRGKRSPA EWIPRPPEK YALTGHRAP VTRVIFHPL FSLFVSTSE DATIKVWDF ETGEYERTL KGHSDAVQD VAFDATGKL LVSCSADMS IKLWDFQQS YECIRTMLG HDHNISSVA FMPAGDFVV SSSRDKTIK MWEVSTGYC VKTFTGHKD WVRVVRPSP DGQLLASCS NDQTIRIWQ VYTKECRAE LRAHEHVVE FVAWAPDSA ANAINEAAG VDNKKGAHM GPFLASCSR DKTIRIWDV GAGVALFVL IGHDNWVRG AVFHPGGKF LVSVSDDKT LRVWDLRNK RCLKTLDAH KHFCTSVDF HKSHPYVIS GSVDLTVKV WECR
- >TC005496 RA
atgaaaatg gttctctct caacgccag cgagaggag cttaacaaa gccatcgct gattacctc ggtagcaac ggttacatg aacgcacta gaagcgttc aaaaaagaa acggacatg cccggcgaa ctcgagcgc aaattcggc ggtcttctc gaaaaaaaa tggacatct gtgatacgt ctccagaag aaagtgatg gaactcgaa tcgaaactc cacgaagcc gaaaaggaa tacatcgag ggggcgcca actcgcggc aaacgcagt ccagccgaa tggatcccc agaccccct gagaaatac gccctgacg gggcaccga gcccccgtc acacgtgtc atattccat ccgttgttt agtctcttt gtatcgaca agtgaagat gctacgatt aaagtctgg gattttgag acaggggaa tacgagagg accctcaaa ggccactcg gatgccgtt caagacgtc gctttcgac gccacagga aaactgcta gtttcctgt agcgccgat atgtcgatt aaattatgg gatttccaa cagagttac gaatgcatc cgaacgatg ctcgggcat gatcacaat atatcatcg gtggcgttc atgccagcg ggggatttt gtcgtttcg tcaagtcgc gataaaacc atcaaaatg tgggaggtt tcgaccggt tattgtgtc aaaaccttt acaggacac aaggattgg gtcagagtt gtgcggcca tcgcccgat ggtcaactc ttggccagt tgttccaac gatcagacc atcagaata tggcaggtt tatacgaag gaatgtcga gctgagttg agagcacac gaacacgtt gtcgaattc gtagcatgg gcacctgat tcggctgcg aatgccatc aatgaagcg gcaggagtc gataataag aagggggca catatggga ccgtttctg gcgtcgtgt tccagggat aagacgatc aggatatgg gatgtgggc gccggcgtg gctctcttc gtgctgatt ggccacgac aattgggtc cgaggggca gttttccat ccgggtggt aaattttta gtgtcggtt agtgacgac aaaacgttg agagtttgg gatttgcga aataagcgc tgtttaaag acgttagat gctcacaaa cacttttgt acgtctgta gatttccac aaatcacat ccttatgtg atttcgggg agtgtagat ctaacggtg aaagtgtgg gaatgccgt taa
- >TC005496 RB
atggttctc tctcaacgc cagcgagag gagcttaac aaagccatc gctgattac ctcggtagc aacggttac atgaacgca ctagaagcg ttcaaaaaa gaaacggac atgcccggc gaactcgag cgcaaattc ggcggtctt ctcgaaaaa aaatggaca tctgtgata cgtctccag aagaaagtg atggaactc gaatcgaaa ctccacgaa gccgaaaag gaatacatc gagggggcg ccaactcgc ggcaaacgc agtccagcc gaatggatc cccagaccc cctgagaaa tacgccctg acggggcac cgagccccc gtcacacgt gtcatattc catccgttg tttagtctc tttgtatcg acaagtgaa gatgctacg attaaagtc tgggatttt gagacaggg gaatacgag aggaccctc aaaggccac tcggatgcc gttcaagac gtcgctttc gacgccaca ggaaaactg ctagtttcc tgtagcgcc gatatgtcg attaaatta tgggatttc caacagagt tacgaatgc atccgaacg atgctcggg catgatcac aatatatca tcggtggcg ttcatgcca gcgggggat tttgtcgtt tcgtcaagt cgcgataaa accatcaaa atgtgggag gtttcgacc ggttattgt gtcaaaacc tttacagga cacaaggat tgggtcaga gttgtgcgg ccatcgccc gatggtcaa ctcttggcc agttgttcc aacgatcag accatcaga atatggcag gtttatacg aaggaatgt cgagctgag ttgagagca cacgaacac gttgtcgaa ttcgtagca tgggcacct gattcggct gcgaatgcc atcaatgaa gcggcagga gtcgataat aagaagggg gcacatatg ggaccgttt ctggcgtcg tgttccagg gataagacg atcaggata tgggatgtg ggcgccggc gtggctctc ttcgtgctg attggccac gacaattgg gtccgaggg gcagttttc catccgggt ggtaaattt ttagtgtcg gttagtgac gacaaaacg ttgagagtt tgggatttg cgaaataag cgctgttta aagacgtta gatgctcac aaacacttt tgtacgtct gtagatttc cacaaatca catccttat gtgatttcg gggagtgta gatctaacg gtgaaagtg tgggaatgc cgttaa
- >TC005496 RA
ccccatccg ttggcaata ctgctctta ctcacgcag ttttgtcat ttcgtatcg ccagtgttc gcgaccgtt acaagtgtc cagtgtctc tgttatcgc cttttaacc aaatcgcag tgaaatagt gtcttggat aatcacttt atcacacct tgtaatgca atagaagat ttctgaaag tcgagcccc ggaacatta acatgaaaa tggttctct ctcaacgcc agcgagagg agcttaaca aagccatcg ctgattacc tcggtagca acggttaca tgaacgcac tagaagcgt tcaaaaaag aaacggaca tgcccggcg aactcgagc gcaaattcg gcggtcttc tcgaaaaaa aatggacat ctgtgatac gtctccaga agaaagtga tggaactcg aatcgaaac tccacgaag ccgaaaagg aatacatcg agggggcgc caactcgcg gcaaacgca gtccagccg aatggatcc ccagacccc ctgagaaat acgccctga cggggcacc gagcccccg tcacacgtg tcatattcc atccgttgt ttagtctct ttgtatcga caagtgaag atgctacga ttaaagtct gggattttg agacagggg aatacgaga ggaccctca aaggccact cggatgccg ttcaagacg tcgctttcg acgccacag gaaaactgc tagtttcct gtagcgccg atatgtcga ttaaattat gggatttcc aacagagtt acgaatgca tccgaacga tgctcgggc atgatcaca atatatcat cggtggcgt tcatgccag cgggggatt ttgtcgttt cgtcaagtc gcgataaaa ccatcaaaa tgtgggagg tttcgaccg gttattgtg tcaaaacct ttacaggac acaaggatt gggtcagag ttgtgcggc catcgcccg atggtcaac tcttggcca gttgttcca acgatcaga ccatcagaa tatggcagg tttatacga aggaatgtc gagctgagt tgagagcac acgaacacg ttgtcgaat tcgtagcat gggcacctg attcggctg cgaatgcca tcaatgaag cggcaggag tcgataata agaaggggg cacatatgg gaccgtttc tggcgtcgt gttccaggg ataagacga tcaggatat gggatgtgg gcgccggcg tggctctct tcgtgctga ttggccacg acaattggg tccgagggg cagttttcc atccgggtg gtaaatttt tagtgtcgg ttagtgacg acaaaacgt tgagagttt gggatttgc gaaataagc gctgtttaa agacgttag atgctcaca aacactttt gtacgtctg tagatttcc acaaatcac atccttatg tgatttcgg ggagtgtag atctaacgg tgaaagtgt gggaatgcc gttaagtta tagtaaaac ttttttttc acggttgca gtgtaattt aattattta agccaaaac atttattcc tttacgaaa ggttttatt tagtgcaaa ttcagttat taaactatt tttttgtga tttgttttg tttcattaa aattggatt tgaggtctg gaatttttt tcttttgag tgtgagtga cttgtagtt tccgtttag aacaaagtt tatttttgt tatattacg atcggtttt tttgtgata tatttttat tagaaactg caagttagt tattcttaa taaatgctt cagatattt aataaacag gcagtggat attgatgaa gttattatt gacacgtct cggtatttt cttgttgtt tattatttt cgtttgttt caaatgtat cgagatgtg tcggaaatg aattcatga aaaattgta gagcttttt cagttcagt tattagaca ctatttata ggggaagaa attaatatc atgttggta ttaattttt tgacatttt aatgacagt cgcaactag gtaactata atccctctc aatgtagat cttgaaaat gtatattgc acaagtccc gcttaatca gtgaaaatt atgtatttt ttttaatac tcatttcat gtttaacaa attgatttc cagtaattg ttttaataa aaacatccc aaaatcgct tgcttacga tgtatagtg gcacaatgt tcgtatata gccttacat tgttttttc gagtaggat atgtgaata ttttcttct gtatataaa aataaaatt gtctcctta ca
- >TC005496 RB
agtttttta tttaaaaaa aaattgtaa cgtaaaatg cctttcccc ctgaacttt ccccaaatt ccccatccg ttggcaata ctgctctta ctcacgcag ttttgtcat ttcgtatcg ccagtgttc gcgaccgtt acaagtgtc cagtgtctc tgttatcgc cttttaacc aaatcgcag tgaaatagt gtcttggat aatcacttt atcacacct tgtaagtaa aatttcccc tttatcact ttcacagcc gcctatccg gcggaaatc ccagacgaa ttgacattg gccaaagca aaatggccg atattgatt ttgtctaaa aggccacaa aacgggaaa atgccattt tccgagaca attttccgc ggtagcggt cattaaacc cgcccccct ttgcccaat cattggctg gggggcctg tcgcgcttt tggcggggc tcaaggggg ctgtcgctg gtccaaagt acagtctga gacgcaagt aatgacgag actagtaaa caaatgaac gttacgtca ttcgagcac ggccaactt tgcaaaacc gtttcctgt catcgttac ggtaacgtg atactcggt ttgggggct tcgccctaa ttctaatcc gtttagaac gattctatc gattaaact ttgggtttg gttaaaatg ttaaattct tgcgtgggc aaatgtcaa aatcgtgtg tttgtgatt gttccaggt aatgcaata gaagatttc tgaaagtcg agccccgga acattaaca tgaaaatgg ttctctctc aacgccagc gagaggagc ttaacaaag ccatcgctg attacctcg gtagcaacg gttacatga acgcactag aagcgttca aaaaagaaa cggacatgc ccggcgaac tcgagcgca aattcggcg gtcttctcg aaaaaaaat ggacatctg tgatacgtc tccagaaga aagtgatgg aactcgaat cgaaactcc acgaagccg aaaaggaat acatcgagg gggcgccaa ctcgcggca aacgcagtc cagccgaat ggatcccca gaccccctg agaaatacg ccctgacgg ggcaccgag cccccgtca cacgtgtca tattccatc cgttgttta gtctctttg tatcgacaa gtgaagatg ctacgatta aagtctggg attttgaga caggggaat acgagagga ccctcaaag gccactcgg atgccgttc aagacgtcg ctttcgacg ccacaggaa aactgctag tttcctgta gcgccgata tgtcgatta aattatggg atttccaac agagttacg aatgcatcc gaacgatgc tcgggcatg atcacaata tatcatcgg tggcgttca tgccagcgg gggattttg tcgtttcgt caagtcgcg ataaaacca tcaaaatgt gggaggttt cgaccggtt attgtgtca aaaccttta caggacaca aggattggg tcagagttg tgcggccat cgcccgatg gtcaactct tggccagtt gttccaacg atcagacca tcagaatat ggcaggttt atacgaagg aatgtcgag ctgagttga gagcacacg aacacgttg tcgaattcg tagcatggg cacctgatt cggctgcga atgccatca atgaagcgg caggagtcg ataataaga agggggcac atatgggac cgtttctgg cgtcgtgtt ccagggata agacgatca ggatatggg atgtgggcg ccggcgtgg ctctcttcg tgctgattg gccacgaca attgggtcc gaggggcag ttttccatc cgggtggta aatttttag tgtcggtta gtgacgaca aaacgttga gagtttggg atttgcgaa ataagcgct gtttaaaga cgttagatg ctcacaaac acttttgta cgtctgtag atttccaca aatcacatc cttatgtga tttcgggga gtgtagatc taacggtga aagtgtggg aatgccgtt aagttatag taaaacttt tttttcacg gttgcagtg taatttaat tatttaagc caaaacatt tattccttt acgaaaggt tttatttag tgcaaattc agttattaa actattttt ttgtgattt gttttgttt cattaaaat tggatttga ggtctggaa tttttttct tttgagtgt gagtgactt gtagtttcc gtttagaac aaagtttat ttttgttat attacgatc ggttttttt gtgatatat ttttattag aaactgcaa gttagttat tcttaataa atgcttcag atatttaat aaacaggca gtggatatt gatgaagtt attattgac acgtctcgg tattttctt gttgtttat tattttcgt ttgtttcaa atgtatcga gatgtgtcg gaaatgaat tcatgaaaa attgtagag ctttttcag ttcagttat tagacacta tttataggg gaagaaatt aatatcatg ttggtatta attttttga cattttaat gacagtcgc aactaggta actataatc cctctcaat gtagatctt gaaaatgta tattgcaca agtcccgct taatcagtg aaaattatg tattttttt taatactca tttcatgtt taacaaatt gatttccag taattgttt taataaaaa catcccaaa atcgcttgc ttacgatgt atagtggca caatgttcg tatatagcc ttacattgt tttttcgag taggatatg tgaatattt tcttctgta tataaaaat aaaattgtc tccttaca
