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Gene information
General information
TC005496 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12104967..12111709
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00869

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Adult morphology
    motility decreased - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC005496 RA
    • >TC005496 RB
    CDS sequence
    • >TC005496 RA
      atgaaaatg gttctctct caacgccag cgagaggag 
      cttaacaaa gccatcgct gattacctc ggtagcaac 
      ggttacatg aacgcacta gaagcgttc aaaaaagaa 
      acggacatg cccggcgaa ctcgagcgc aaattcggc 
      ggtcttctc gaaaaaaaa tggacatct gtgatacgt 
      ctccagaag aaagtgatg gaactcgaa tcgaaactc 
      cacgaagcc gaaaaggaa tacatcgag ggggcgcca 
      actcgcggc aaacgcagt ccagccgaa tggatcccc 
      agaccccct gagaaatac gccctgacg gggcaccga 
      gcccccgtc acacgtgtc atattccat ccgttgttt 
      agtctcttt gtatcgaca agtgaagat gctacgatt 
      aaagtctgg gattttgag acaggggaa tacgagagg 
      accctcaaa ggccactcg gatgccgtt caagacgtc 
      gctttcgac gccacagga aaactgcta gtttcctgt 
      agcgccgat atgtcgatt aaattatgg gatttccaa 
      cagagttac gaatgcatc cgaacgatg ctcgggcat 
      gatcacaat atatcatcg gtggcgttc atgccagcg 
      ggggatttt gtcgtttcg tcaagtcgc gataaaacc 
      atcaaaatg tgggaggtt tcgaccggt tattgtgtc 
      aaaaccttt acaggacac aaggattgg gtcagagtt 
      gtgcggcca tcgcccgat ggtcaactc ttggccagt 
      tgttccaac gatcagacc atcagaata tggcaggtt 
      tatacgaag gaatgtcga gctgagttg agagcacac 
      gaacacgtt gtcgaattc gtagcatgg gcacctgat 
      tcggctgcg aatgccatc aatgaagcg gcaggagtc 
      gataataag aagggggca catatggga ccgtttctg 
      gcgtcgtgt tccagggat aagacgatc aggatatgg 
      gatgtgggc gccggcgtg gctctcttc gtgctgatt 
      ggccacgac aattgggtc cgaggggca gttttccat 
      ccgggtggt aaattttta gtgtcggtt agtgacgac 
      aaaacgttg agagtttgg gatttgcga aataagcgc 
      tgtttaaag acgttagat gctcacaaa cacttttgt 
      acgtctgta gatttccac aaatcacat ccttatgtg 
      atttcgggg agtgtagat ctaacggtg aaagtgtgg 
      gaatgccgt taa
    • >TC005496 RB
      atggttctc tctcaacgc cagcgagag gagcttaac 
      aaagccatc gctgattac ctcggtagc aacggttac 
      atgaacgca ctagaagcg ttcaaaaaa gaaacggac 
      atgcccggc gaactcgag cgcaaattc ggcggtctt 
      ctcgaaaaa aaatggaca tctgtgata cgtctccag 
      aagaaagtg atggaactc gaatcgaaa ctccacgaa 
      gccgaaaag gaatacatc gagggggcg ccaactcgc 
      ggcaaacgc agtccagcc gaatggatc cccagaccc 
      cctgagaaa tacgccctg acggggcac cgagccccc 
      gtcacacgt gtcatattc catccgttg tttagtctc 
      tttgtatcg acaagtgaa gatgctacg attaaagtc 
      tgggatttt gagacaggg gaatacgag aggaccctc 
      aaaggccac tcggatgcc gttcaagac gtcgctttc 
      gacgccaca ggaaaactg ctagtttcc tgtagcgcc 
      gatatgtcg attaaatta tgggatttc caacagagt 
      tacgaatgc atccgaacg atgctcggg catgatcac 
      aatatatca tcggtggcg ttcatgcca gcgggggat 
      tttgtcgtt tcgtcaagt cgcgataaa accatcaaa 
      atgtgggag gtttcgacc ggttattgt gtcaaaacc 
      tttacagga cacaaggat tgggtcaga gttgtgcgg 
      ccatcgccc gatggtcaa ctcttggcc agttgttcc 
      aacgatcag accatcaga atatggcag gtttatacg 
      aaggaatgt cgagctgag ttgagagca cacgaacac 
      gttgtcgaa ttcgtagca tgggcacct gattcggct 
      gcgaatgcc atcaatgaa gcggcagga gtcgataat 
      aagaagggg gcacatatg ggaccgttt ctggcgtcg 
      tgttccagg gataagacg atcaggata tgggatgtg 
      ggcgccggc gtggctctc ttcgtgctg attggccac 
      gacaattgg gtccgaggg gcagttttc catccgggt 
      ggtaaattt ttagtgtcg gttagtgac gacaaaacg 
      ttgagagtt tgggatttg cgaaataag cgctgttta 
      aagacgtta gatgctcac aaacacttt tgtacgtct 
      gtagatttc cacaaatca catccttat gtgatttcg 
      gggagtgta gatctaacg gtgaaagtg tgggaatgc 
    mRNA sequence
    • >TC005496 RA
      ccccatccg ttggcaata ctgctctta ctcacgcag 
      ttttgtcat ttcgtatcg ccagtgttc gcgaccgtt 
      acaagtgtc cagtgtctc tgttatcgc cttttaacc 
      aaatcgcag tgaaatagt gtcttggat aatcacttt 
      atcacacct tgtaatgca atagaagat ttctgaaag 
      tcgagcccc ggaacatta acatgaaaa tggttctct 
      ctcaacgcc agcgagagg agcttaaca aagccatcg 
      ctgattacc tcggtagca acggttaca tgaacgcac 
      tagaagcgt tcaaaaaag aaacggaca tgcccggcg 
      aactcgagc gcaaattcg gcggtcttc tcgaaaaaa 
      aatggacat ctgtgatac gtctccaga agaaagtga 
      tggaactcg aatcgaaac tccacgaag ccgaaaagg 
      aatacatcg agggggcgc caactcgcg gcaaacgca 
      gtccagccg aatggatcc ccagacccc ctgagaaat 
      acgccctga cggggcacc gagcccccg tcacacgtg 
      tcatattcc atccgttgt ttagtctct ttgtatcga 
      caagtgaag atgctacga ttaaagtct gggattttg 
      agacagggg aatacgaga ggaccctca aaggccact 
      cggatgccg ttcaagacg tcgctttcg acgccacag 
      gaaaactgc tagtttcct gtagcgccg atatgtcga 
      ttaaattat gggatttcc aacagagtt acgaatgca 
      tccgaacga tgctcgggc atgatcaca atatatcat 
      cggtggcgt tcatgccag cgggggatt ttgtcgttt 
      cgtcaagtc gcgataaaa ccatcaaaa tgtgggagg 
      tttcgaccg gttattgtg tcaaaacct ttacaggac 
      acaaggatt gggtcagag ttgtgcggc catcgcccg 
      atggtcaac tcttggcca gttgttcca acgatcaga 
      ccatcagaa tatggcagg tttatacga aggaatgtc 
      gagctgagt tgagagcac acgaacacg ttgtcgaat 
      tcgtagcat gggcacctg attcggctg cgaatgcca 
      tcaatgaag cggcaggag tcgataata agaaggggg 
      cacatatgg gaccgtttc tggcgtcgt gttccaggg 
      ataagacga tcaggatat gggatgtgg gcgccggcg 
      tggctctct tcgtgctga ttggccacg acaattggg 
      tccgagggg cagttttcc atccgggtg gtaaatttt 
      tagtgtcgg ttagtgacg acaaaacgt tgagagttt 
      gggatttgc gaaataagc gctgtttaa agacgttag 
      atgctcaca aacactttt gtacgtctg tagatttcc 
      acaaatcac atccttatg tgatttcgg ggagtgtag 
      atctaacgg tgaaagtgt gggaatgcc gttaagtta 
      tagtaaaac ttttttttc acggttgca gtgtaattt 
      aattattta agccaaaac atttattcc tttacgaaa 
      ggttttatt tagtgcaaa ttcagttat taaactatt 
      tttttgtga tttgttttg tttcattaa aattggatt 
      tgaggtctg gaatttttt tcttttgag tgtgagtga 
      cttgtagtt tccgtttag aacaaagtt tatttttgt 
      tatattacg atcggtttt tttgtgata tatttttat 
      tagaaactg caagttagt tattcttaa taaatgctt 
      cagatattt aataaacag gcagtggat attgatgaa 
      gttattatt gacacgtct cggtatttt cttgttgtt 
      tattatttt cgtttgttt caaatgtat cgagatgtg 
      tcggaaatg aattcatga aaaattgta gagcttttt 
      cagttcagt tattagaca ctatttata ggggaagaa 
      attaatatc atgttggta ttaattttt tgacatttt 
      aatgacagt cgcaactag gtaactata atccctctc 
      aatgtagat cttgaaaat gtatattgc acaagtccc 
      gcttaatca gtgaaaatt atgtatttt ttttaatac 
      tcatttcat gtttaacaa attgatttc cagtaattg 
      ttttaataa aaacatccc aaaatcgct tgcttacga 
      tgtatagtg gcacaatgt tcgtatata gccttacat 
      tgttttttc gagtaggat atgtgaata ttttcttct 
      gtatataaa aataaaatt gtctcctta ca
    • >TC005496 RB
      agtttttta tttaaaaaa aaattgtaa cgtaaaatg 
      cctttcccc ctgaacttt ccccaaatt ccccatccg 
      ttggcaata ctgctctta ctcacgcag ttttgtcat 
      ttcgtatcg ccagtgttc gcgaccgtt acaagtgtc 
      cagtgtctc tgttatcgc cttttaacc aaatcgcag 
      tgaaatagt gtcttggat aatcacttt atcacacct 
      tgtaagtaa aatttcccc tttatcact ttcacagcc 
      gcctatccg gcggaaatc ccagacgaa ttgacattg 
      gccaaagca aaatggccg atattgatt ttgtctaaa 
      aggccacaa aacgggaaa atgccattt tccgagaca 
      attttccgc ggtagcggt cattaaacc cgcccccct 
      ttgcccaat cattggctg gggggcctg tcgcgcttt 
      tggcggggc tcaaggggg ctgtcgctg gtccaaagt 
      acagtctga gacgcaagt aatgacgag actagtaaa 
      caaatgaac gttacgtca ttcgagcac ggccaactt 
      tgcaaaacc gtttcctgt catcgttac ggtaacgtg 
      atactcggt ttgggggct tcgccctaa ttctaatcc 
      gtttagaac gattctatc gattaaact ttgggtttg 
      gttaaaatg ttaaattct tgcgtgggc aaatgtcaa 
      aatcgtgtg tttgtgatt gttccaggt aatgcaata 
      gaagatttc tgaaagtcg agccccgga acattaaca 
      tgaaaatgg ttctctctc aacgccagc gagaggagc 
      ttaacaaag ccatcgctg attacctcg gtagcaacg 
      gttacatga acgcactag aagcgttca aaaaagaaa 
      cggacatgc ccggcgaac tcgagcgca aattcggcg 
      gtcttctcg aaaaaaaat ggacatctg tgatacgtc 
      tccagaaga aagtgatgg aactcgaat cgaaactcc 
      acgaagccg aaaaggaat acatcgagg gggcgccaa 
      ctcgcggca aacgcagtc cagccgaat ggatcccca 
      gaccccctg agaaatacg ccctgacgg ggcaccgag 
      cccccgtca cacgtgtca tattccatc cgttgttta 
      gtctctttg tatcgacaa gtgaagatg ctacgatta 
      aagtctggg attttgaga caggggaat acgagagga 
      ccctcaaag gccactcgg atgccgttc aagacgtcg 
      ctttcgacg ccacaggaa aactgctag tttcctgta 
      gcgccgata tgtcgatta aattatggg atttccaac 
      agagttacg aatgcatcc gaacgatgc tcgggcatg 
      atcacaata tatcatcgg tggcgttca tgccagcgg 
      gggattttg tcgtttcgt caagtcgcg ataaaacca 
      tcaaaatgt gggaggttt cgaccggtt attgtgtca 
      aaaccttta caggacaca aggattggg tcagagttg 
      tgcggccat cgcccgatg gtcaactct tggccagtt 
      gttccaacg atcagacca tcagaatat ggcaggttt 
      atacgaagg aatgtcgag ctgagttga gagcacacg 
      aacacgttg tcgaattcg tagcatggg cacctgatt 
      cggctgcga atgccatca atgaagcgg caggagtcg 
      ataataaga agggggcac atatgggac cgtttctgg 
      cgtcgtgtt ccagggata agacgatca ggatatggg 
      atgtgggcg ccggcgtgg ctctcttcg tgctgattg 
      gccacgaca attgggtcc gaggggcag ttttccatc 
      cgggtggta aatttttag tgtcggtta gtgacgaca 
      aaacgttga gagtttggg atttgcgaa ataagcgct 
      gtttaaaga cgttagatg ctcacaaac acttttgta 
      cgtctgtag atttccaca aatcacatc cttatgtga 
      tttcgggga gtgtagatc taacggtga aagtgtggg 
      aatgccgtt aagttatag taaaacttt tttttcacg 
      gttgcagtg taatttaat tatttaagc caaaacatt 
      tattccttt acgaaaggt tttatttag tgcaaattc 
      agttattaa actattttt ttgtgattt gttttgttt 
      cattaaaat tggatttga ggtctggaa tttttttct 
      tttgagtgt gagtgactt gtagtttcc gtttagaac 
      aaagtttat ttttgttat attacgatc ggttttttt 
      gtgatatat ttttattag aaactgcaa gttagttat 
      tcttaataa atgcttcag atatttaat aaacaggca 
      gtggatatt gatgaagtt attattgac acgtctcgg 
      tattttctt gttgtttat tattttcgt ttgtttcaa 
      atgtatcga gatgtgtcg gaaatgaat tcatgaaaa 
      attgtagag ctttttcag ttcagttat tagacacta 
      tttataggg gaagaaatt aatatcatg ttggtatta 
      attttttga cattttaat gacagtcgc aactaggta 
      actataatc cctctcaat gtagatctt gaaaatgta 
      tattgcaca agtcccgct taatcagtg aaaattatg 
      tattttttt taatactca tttcatgtt taacaaatt 
      gatttccag taattgttt taataaaaa catcccaaa 
      atcgcttgc ttacgatgt atagtggca caatgttcg 
      tatatagcc ttacattgt tttttcgag taggatatg 
      tgaatattt tcttctgta tataaaaat aaaattgtc 

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY