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Gene information
General information
TC005493 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 675288..685547
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005493
    iBeetle screen iB_00868
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005493 RA
      MEVTFGLAL LALLPAALS ATNITAYTR ALPSELKPT 
      VRAALGAEL NAPRPCQSH DAVPCPPNK YRRPSGECN 
      NVRHAHWGT RGVPFLRLL PPDYADGRS QPRQSVTTH 
      ALPSPLDVA VTLQTVPAT PHESVTALL GAWSELLLH 
      DLASTGNLK SQDCCSDGR KHGECYGRV GHGQCREYM 
      RTLPAIDMD DCDFEYRNQ MNLASSFLD ASAIYGNTD 
      QQVEKLRTY DAGLVNVSA CTSCRSNAL YSAILKEHN 
      RVAINLAQL NRHWTDETL FLESKRIVT AMLQHITYN 
      EFLPIVLGN EAIVQSDLQ LQSHGRFSK YSSSHRAGV 
      YNEVAMSAL PALLSMIPT TLMNETVES FAEMVDVLI 
      RTPAQSPSM HMMVPLRAD WDTAALFVH MGRDHGIPP 
      YAHYLSKCQ NLTIEKFDD FTKAGISPE LLKALKYLY 
      QVPEDVDLL VGSLLERPI PGAIVGGTL ECLLREQFI 
      LLKQSDRFW YENDLPPSS LTTEQLAEI KKVTLAGLL 
      CANTDDLDK IQPKAFVQE DPYLNARLS CDQHPLPQL 
      TPWLEMDHM ADLSEDLLM EALVKAEQE VLERRKTEY 
      ELWATVGGI DPKSPAGTA ASFSKANKQ ALKLANTSL 
      LFEFASNEI INSLMHRRR KRQIFDNNV ISSVFRDEL 
      SDGLQNVDI SSLIPANAI DLDTHCEET GPCDPNTPY 
      RTMTGHCNN LRNPNWGKS LTTFSRLLP SAYDDGISK 
      PRLLGATGV PLPSPRIVS TLIHPDISN LHSRYTLMI 
      MQYGQFLDH DLTMTPIHK GFHESIPDC RSCDSPRTV 
      HPECNPFPI PPSDHYYPE INITSGQRM CFPFMRSLP 
      GQLHLGPRE QVNQNTAFL DASQIYGEN PCVLKELKG 
      YGGRMNCTQ RPLKLKDLL PQSDHHPEC KAGSGLCFI 
      AGDGRASEQ PGLTVIHTI FMREHNRMV EGLKQVNPH 
      WDDQKLFEH ARRINIAAN QHITYNEWL PRILSWNAV 
      NLYGLKLLP QGYYKDYNP SCNPAILTE FAAAAFRIG 
      HSLLRPHIP RLSPSYQII DPPILLRDG FFKPDMLLQ 
      TGMVDEIAR GLVSTPMET LDQFITGEV TNHLFEDRK 
      IPFSGVDLI ALNIQRARD HGIPSYNNY RALCNLKRA 
      SNFEDLARE IPPEVIARF KRIYPTVDD IDLFPGGLS 
      ERPLQGGLV GPTFACIIA IQFRQLRKC DRFWYENHD 
      PTVRFSEAQ LAEIRKVTL AKIICDNLD VHGDMQRAA 
      FDLPSNFLN PRVPCDSLP KLDFGAWRE SVGSGCVIG 
      DRMVEVGQS AFPTPCTSC ICTQEGGQC ASLRVTDCS 
      QLLREWPKE VVLRDDVCS AQCGFLLRN EGPQPGLNT 
      DLTPPPRHL RARQLGRPP RIPSRGFNG FGEFKLPDL 
      SVFVAK
    CDS sequence
    • >TC005493 RA
      atggaggtg acgtttgga ttagccctc ctggcgctc 
      ctccccgcc gccctctcc gccaccaac atcaccgcc 
      tacacgcgc gccctccct tccgaactc aaaccaacg 
      gtgagggcg gccctgggg gcggagctg aacgcgccc 
      cgtccttgc caatcgcac gatgccgtc ccgtgtccg 
      cccaataaa taccggcgg ccgtcgggc gaatgtaac 
      aatgtacga cacgcccat tggggcacg cggggtgtg 
      ccgtttttg cgactcctc ccaccggac tacgccgat 
      gggcggagc cagccgcgc caatcggtc acgacgcac 
      gcgctgccg agcccattg gacgtcgct gtgaccttg 
      caaacggtg ccggccacg ccccatgag tcagtcacc 
      gcccttttg ggggcgtgg agcgaatta ttactacat 
      gatttggca agcactggc aatttaaag agccaggat 
      tgttgcagc gatgggcgg aagcatggc gagtgctat 
      gggagggtg gggcatgga cagtgtcgg gaatatatg 
      cggacgcta cccgccatt gacatggac gactgcgat 
      tttgagtac cgaaatcaa atgaatttg gcttcgtcc 
      tttttggat gcgtcggca atttacgga aataccgac 
      caacaagtc gaaaaatta cgaacgtac gacgcgggt 
      ctcgtcaat gtctcggcg tgcacctcg tgtcgctcc 
      aacgcactc tattcggcc attttgaag gagcacaat 
      cgagttgcc attaactta gcccagttg aatcgccac 
      tggactgac gaaactttg ttcctagag agtaaacga 
      atcgtgacg gccatgcta cagcacatc acctacaac 
      gagtttctc cccattgta ttgggcaat gaagccatc 
      gtacagtcc gacctccaa ttgcaaagt catgggcgg 
      ttctctaaa tactcgagc tcgcacagg gccggtgtc 
      tacaatgag gtggccatg agcgctctc cccgccctc 
      ctgtcaatg atccccacc actttgatg aacgaaaca 
      gttgaaagt ttcgccgaa atggtggac gtcttgatc 
      agaaccccg gcccaaagc cccagtatg cacatgatg 
      gtaccacta cgtgcggat tgggacacg gcggccctt 
      ttcgtccat atgggccgc gaccatggg attcccccc 
      tacgcccat tatctctcc aagtgccaa aacctcaca 
      attgagaaa ttcgacgac tttaccaag gctggaatc 
      agccccgaa ctgctcaaa gccttgaag tacctctac 
      caagtacca gaggacgta gaccttttg gttggttcc 
      ttgctggaa cgcccaatt ccgggggca atcgttggc 
      ggaaccctt gaatgtctc ctccgtgag caatttatt 
      cttctgaag caaagcgac cgcttctgg tacgaaaat 
      gacctacct ccaagttcg ctaacaacg gaacaactt 
      gccgaaatc aagaaggtc acccttgct gggttattg 
      tgcgcaaac accgacgat ttggacaag atccaacct 
      aaagccttc gtccaggag gatccgtac ttgaacgcc 
      cgcttgtcc tgcgaccaa caccccctc ccccagctc 
      accccctgg ctggagatg gaccacatg gccgacctc 
      tccgaagac ctgctcatg gaagccctc gtcaaagcc 
      gagcaagag gtcctagag cgtcgcaaa accgaatac 
      gaactgtgg gccaccgtt ggcggaatc gaccccaag 
      tcacctgca gggaccgcc gcctccttc agcaaggcc 
      aacaaacag gccctcaaa ctcgcaaac acgtctcta 
      ttgtttgaa ttcgcctca aacgaaatc atcaattct 
      ttaatgcac cggaggcgc aaacgccaa attttcgac 
      aataacgtt atttcatcc gtctttaga gacgaacta 
      tcggatgga ttacaaaac gttgatatt tcgtctcta 
      atcccggcc aacgctatt gatttggac acacattgt 
      gaggagacc ggtccttgc gacccgaac acgccttat 
      aggacgatg actggtcat tgtaacaat ttgcggaac 
      cctaattgg ggcaaaagt ctgactacg ttcagtcgg 
      ctcctccca agcgcctac gacgacgga atatctaag 
      ccacgcctc ttgggcgcg accggcgtg cctttgccc 
      tccccccgc atcgtctcc accctcatc caccccgac 
      atcagcaac ctccactcc cgttacacc ctcatgatc 
      atgcaatat ggccaattc ctggaccat gacttgacc 
      atgacgccc atccataag ggtttccac gagtctatc 
      ccggattgc cggtcatgt gacagtccc cggaccgtt 
      cacccggaa tgtaaccct ttccccatc ccgccctct 
      gatcactac tacccggaa attaacata acgtccggt 
      caaagaatg tgcttcccg tttatgagg tccctccct 
      ggacaactc cacctaggg ccacgggaa caagtcaat 
      caaaacaca gccttcttg gacgcgtcc caaatctac 
      ggggaaaac ccgtgcgtt ttgaaagaa ttgaaaggt 
      tacggtggg cgtatgaac tgcacgcaa cgccccctc 
      aaactcaag gacctcctc ccgcaaagt gaccaccac 
      ccagaatgc aaggctggc agtgggttg tgtttcatc 
      gcaggcgat gggcgggcc tcagaacag cccggttta 
      accgtaatt cacacgatt ttcatgcgg gaacataac 
      cggatggtg gaagggtta aaacaagtg aaccctcac 
      tgggacgac cagaagtta ttcgaacac gcgcgccgt 
      attaacata gccgccaac caacatata acttacaac 
      gaatggtta cctcgcatt ttgagctgg aatgctgtt 
      aacttgtac ggcttgaag ctcctcccc cagggttac 
      tacaaggat tacaacccg tcgtgcaac cccgccatt 
      ttgactgaa ttcgcagcg gcggcgttc cgcattggt 
      cactcgtta ctccgcccc catatcccc cgtctaagc 
      ccctcctac cagatcatc gacccgccc attctcctc 
      cgtgacggt ttcttcaaa cccgatatg ctcctccaa 
      accggaatg gtggacgaa atcgcccga ggcctcgtc 
      tccacacca atggaaaca ctcgaccaa ttcataacc 
      ggggaagtg accaatcac ctgttcgaa gatcggaaa 
      atccccttc tcgggggtg gacctaatc gccctcaac 
      atccaacgc gcgcgcgac cacggaatc ccctcctac 
      aacaactac cgcgcctta tgcaacctc aaacgcgcc 
      tcaaacttt gaggacctg gccagggaa atccccccc 
      gaagtgatc gctcgcttc aagcgaatt tacccgacc 
      gtcgacgac atcgacctg ttccccggc ggtttaagc 
      gaacgcccg cttcaagga ggcctcgtc ggccccacc 
      ttcgcctgc atcatagca atccagttc cgccaactg 
      cgaaaatgc gaccgcttc tggtacgaa aaccacgac 
      ccgactgtt cgtttctcc gaagcacaa ctcgcggaa 
      atccgtaaa gtcaccctc gcgaaaatc atctgtgat 
      aatctagac gtccatggc gacatgcag cgcgccgcc 
      tttgacctc cccagcaat ttcctcaat ccgcgagtg 
      ccgtgcgac tccctcccg aagctcgat ttcggggcg 
      tggcgcgag tcggtgggg tcggggtgt gtcattggc 
      gacaggatg gtagaagtg ggccaatcg gcgttcccc 
      acgccgtgc acgagctgt atttgcacg caggagggc 
      gggcaatgt gcgtcgttg cgggtcacc gattgctcg 
      cagcttttg agggagtgg ccaaaggag gtggtgcta 
      agggatgat gtgtgttcg gcccaatgc gggttcttg 
      ttgcggaat gagggcccg cagccgggg ctgaacacc 
      gatttgacg ccgcccccg aggcatctg agggcgcgg 
      cagttggga cgaccgccc aggatcccg agtaggggg 
      tttaatggg ttcggggag tttaaactg cccgatttg 
      tcggtgttt gtggccaag tga
    mRNA sequence
    • >TC005493 RA
      taagctcct cgtggattt ccagagagc acaagagtt 
      cgcgttgtg gaacagacg agatctgtt tgttggttg 
      ttgtcttcc gagcgtgag cgattatgt gtgtgcact 
      tatcttggt tttgaactt ccctttctg tgttttttt 
      tctgcgaca tggaggtga cgtttggat tagccctcc 
      tggcgctcc tccccgccg ccctctccg ccaccaaca 
      tcaccgcct acacgcgcg ccctccctt ccgaactca 
      aaccaacgg tgagggcgg ccctggggg cggagctga 
      acgcgcccc gtccttgcc aatcgcacg atgccgtcc 
      cgtgtccgc ccaataaat accggcggc cgtcgggcg 
      aatgtaaca atgtacgac acgcccatt ggggcacgc 
      ggggtgtgc cgtttttgc gactcctcc caccggact 
      acgccgatg ggcggagcc agccgcgcc aatcggtca 
      cgacgcacg cgctgccga gcccattgg acgtcgctg 
      tgaccttgc aaacggtgc cggccacgc cccatgagt 
      cagtcaccg cccttttgg gggcgtgga gcgaattat 
      tactacatg atttggcaa gcactggca atttaaaga 
      gccaggatt gttgcagcg atgggcgga agcatggcg 
      agtgctatg ggagggtgg ggcatggac agtgtcggg 
      aatatatgc ggacgctac ccgccattg acatggacg 
      actgcgatt ttgagtacc gaaatcaaa tgaatttgg 
      cttcgtcct ttttggatg cgtcggcaa tttacggaa 
      ataccgacc aacaagtcg aaaaattac gaacgtacg 
      acgcgggtc tcgtcaatg tctcggcgt gcacctcgt 
      gtcgctcca acgcactct attcggcca ttttgaagg 
      agcacaatc gagttgcca ttaacttag cccagttga 
      atcgccact ggactgacg aaactttgt tcctagaga 
      gtaaacgaa tcgtgacgg ccatgctac agcacatca 
      cctacaacg agtttctcc ccattgtat tgggcaatg 
      aagccatcg tacagtccg acctccaat tgcaaagtc 
      atgggcggt tctctaaat actcgagct cgcacaggg 
      ccggtgtct acaatgagg tggccatga gcgctctcc 
      ccgccctcc tgtcaatga tccccacca ctttgatga 
      acgaaacag ttgaaagtt tcgccgaaa tggtggacg 
      tcttgatca gaaccccgg cccaaagcc ccagtatgc 
      acatgatgg taccactac gtgcggatt gggacacgg 
      cggcccttt tcgtccata tgggccgcg accatggga 
      ttcccccct acgcccatt atctctcca agtgccaaa 
      acctcacaa ttgagaaat tcgacgact ttaccaagg 
      ctggaatca gccccgaac tgctcaaag ccttgaagt 
      acctctacc aagtaccag aggacgtag accttttgg 
      ttggttcct tgctggaac gcccaattc cgggggcaa 
      tcgttggcg gaacccttg aatgtctcc tccgtgagc 
      aatttattc ttctgaagc aaagcgacc gcttctggt 
      acgaaaatg acctacctc caagttcgc taacaacgg 
      aacaacttg ccgaaatca agaaggtca cccttgctg 
      ggttattgt gcgcaaaca ccgacgatt tggacaaga 
      tccaaccta aagccttcg tccaggagg atccgtact 
      tgaacgccc gcttgtcct gcgaccaac accccctcc 
      cccagctca ccccctggc tggagatgg accacatgg 
      ccgacctct ccgaagacc tgctcatgg aagccctcg 
      tcaaagccg agcaagagg tcctagagc gtcgcaaaa 
      ccgaatacg aactgtggg ccaccgttg gcggaatcg 
      accccaagt cacctgcag ggaccgccg cctccttca 
      gcaaggcca acaaacagg ccctcaaac tcgcaaaca 
      cgtctctat tgtttgaat tcgcctcaa acgaaatca 
      tcaattctt taatgcacc ggaggcgca aacgccaaa 
      ttttcgaca ataacgtta tttcatccg tctttagag 
      acgaactat cggatggat tacaaaacg ttgatattt 
      cgtctctaa tcccggcca acgctattg atttggaca 
      cacattgtg aggagaccg gtccttgcg acccgaaca 
      cgccttata ggacgatga ctggtcatt gtaacaatt 
      tgcggaacc ctaattggg gcaaaagtc tgactacgt 
      tcagtcggc tcctcccaa gcgcctacg acgacggaa 
      tatctaagc cacgcctct tgggcgcga ccggcgtgc 
      ctttgccct ccccccgca tcgtctcca ccctcatcc 
      accccgaca tcagcaacc tccactccc gttacaccc 
      tcatgatca tgcaatatg gccaattcc tggaccatg 
      acttgacca tgacgccca tccataagg gtttccacg 
      agtctatcc cggattgcc ggtcatgtg acagtcccc 
      ggaccgttc acccggaat gtaaccctt tccccatcc 
      cgccctctg atcactact acccggaaa ttaacataa 
      cgtccggtc aaagaatgt gcttcccgt ttatgaggt 
      ccctccctg gacaactcc acctagggc cacgggaac 
      aagtcaatc aaaacacag ccttcttgg acgcgtccc 
      aaatctacg gggaaaacc cgtgcgttt tgaaagaat 
      tgaaaggtt acggtgggc gtatgaact gcacgcaac 
      gccccctca aactcaagg acctcctcc cgcaaagtg 
      accaccacc cagaatgca aggctggca gtgggttgt 
      gtttcatcg caggcgatg ggcgggcct cagaacagc 
      ccggtttaa ccgtaattc acacgattt tcatgcggg 
      aacataacc ggatggtgg aagggttaa aacaagtga 
      accctcact gggacgacc agaagttat tcgaacacg 
      cgcgccgta ttaacatag ccgccaacc aacatataa 
      cttacaacg aatggttac ctcgcattt tgagctgga 
      atgctgtta acttgtacg gcttgaagc tcctccccc 
      agggttact acaaggatt acaacccgt cgtgcaacc 
      ccgccattt tgactgaat tcgcagcgg cggcgttcc 
      gcattggtc actcgttac tccgccccc atatccccc 
      gtctaagcc cctcctacc agatcatcg acccgccca 
      ttctcctcc gtgacggtt tcttcaaac ccgatatgc 
      tcctccaaa ccggaatgg tggacgaaa tcgcccgag 
      gcctcgtct ccacaccaa tggaaacac tcgaccaat 
      tcataaccg gggaagtga ccaatcacc tgttcgaag 
      atcggaaaa tccccttct cgggggtgg acctaatcg 
      ccctcaaca tccaacgcg cgcgcgacc acggaatcc 
      cctcctaca acaactacc gcgccttat gcaacctca 
      aacgcgcct caaactttg aggacctgg ccagggaaa 
      tcccccccg aagtgatcg ctcgcttca agcgaattt 
      acccgaccg tcgacgaca tcgacctgt tccccggcg 
      gtttaagcg aacgcccgc ttcaaggag gcctcgtcg 
      gccccacct tcgcctgca tcatagcaa tccagttcc 
      gccaactgc gaaaatgcg accgcttct ggtacgaaa 
      accacgacc cgactgttc gtttctccg aagcacaac 
      tcgcggaaa tccgtaaag tcaccctcg cgaaaatca 
      tctgtgata atctagacg tccatggcg acatgcagc 
      gcgccgcct ttgacctcc ccagcaatt tcctcaatc 
      cgcgagtgc cgtgcgact ccctcccga agctcgatt 
      tcggggcgt ggcgcgagt cggtggggt cggggtgtg 
      tcattggcg acaggatgg tagaagtgg gccaatcgg 
      cgttcccca cgccgtgca cgagctgta tttgcacgc 
      aggagggcg ggcaatgtg cgtcgttgc gggtcaccg 
      attgctcgc agcttttga gggagtggc caaaggagg 
      tggtgctaa gggatgatg tgtgttcgg cccaatgcg 
      ggttcttgt tgcggaatg agggcccgc agccggggc 
      tgaacaccg atttgacgc cgcccccga ggcatctga 
      gggcgcggc agttgggac gaccgccca ggatcccga 
      gtagggggt ttaatgggt tcggggagt ttaaactgc 
      ccgatttgt cggtgtttg tggccaagt gagcgaaag 
      tggacgtta tttgtggat ttttttcga tgaacgagg 
      gactaaaag gggtggtaa agcctcggg gaggttatt 
      tataaaaat ttgattgtg attcgagaa gagagagaa 
      tgttaccaa ctttagggt taatctaat ttgtgtctt 
      ctttttgta ctttatttt attgttttt atacagtgt 
      acactgcct tctgtatta ttattctgt tgttaatac 
      ttgtactcc tctgactat gcattatta aactaccat 
      tattttttt attccgtgt ccagcttac caataaaaa 
      cctacaatt ttt