| General information | |||
| TC005493 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12122410..12132669 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005493 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00868 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
- >TC005493 RA
MEVTFGLAL LALLPAALS ATNITAYTR ALPSELKPT VRAALGAEL NAPRPCQSH DAVPCPPNK YRRPSGECN NVRHAHWGT RGVPFLRLL PPDYADGRS QPRQSVTTH ALPSPLDVA VTLQTVPAT PHESVTALL GAWSELLLH DLASTGNLK SQDCCSDGR KHGECYGRV GHGQCREYM RTLPAIDMD DCDFEYRNQ MNLASSFLD ASAIYGNTD QQVEKLRTY DAGLVNVSA CTSCRSNAL YSAILKEHN RVAINLAQL NRHWTDETL FLESKRIVT AMLQHITYN EFLPIVLGN EAIVQSDLQ LQSHGRFSK YSSSHRAGV YNEVAMSAL PALLSMIPT TLMNETVES FAEMVDVLI RTPAQSPSM HMMVPLRAD WDTAALFVH MGRDHGIPP YAHYLSKCQ NLTIEKFDD FTKAGISPE LLKALKYLY QVPEDVDLL VGSLLERPI PGAIVGGTL ECLLREQFI LLKQSDRFW YENDLPPSS LTTEQLAEI KKVTLAGLL CANTDDLDK IQPKAFVQE DPYLNARLS CDQHPLPQL TPWLEMDHM ADLSEDLLM EALVKAEQE VLERRKTEY ELWATVGGI DPKSPAGTA ASFSKANKQ ALKLANTSL LFEFASNEI INSLMHRRR KRQIFDNNV ISSVFRDEL SDGLQNVDI SSLIPANAI DLDTHCEET GPCDPNTPY RTMTGHCNN LRNPNWGKS LTTFSRLLP SAYDDGISK PRLLGATGV PLPSPRIVS TLIHPDISN LHSRYTLMI MQYGQFLDH DLTMTPIHK GFHESIPDC RSCDSPRTV HPECNPFPI PPSDHYYPE INITSGQRM CFPFMRSLP GQLHLGPRE QVNQNTAFL DASQIYGEN PCVLKELKG YGGRMNCTQ RPLKLKDLL PQSDHHPEC KAGSGLCFI AGDGRASEQ PGLTVIHTI FMREHNRMV EGLKQVNPH WDDQKLFEH ARRINIAAN QHITYNEWL PRILSWNAV NLYGLKLLP QGYYKDYNP SCNPAILTE FAAAAFRIG HSLLRPHIP RLSPSYQII DPPILLRDG FFKPDMLLQ TGMVDEIAR GLVSTPMET LDQFITGEV TNHLFEDRK IPFSGVDLI ALNIQRARD HGIPSYNNY RALCNLKRA SNFEDLARE IPPEVIARF KRIYPTVDD IDLFPGGLS ERPLQGGLV GPTFACIIA IQFRQLRKC DRFWYENHD PTVRFSEAQ LAEIRKVTL AKIICDNLD VHGDMQRAA FDLPSNFLN PRVPCDSLP KLDFGAWRE SVGSGCVIG DRMVEVGQS AFPTPCTSC ICTQEGGQC ASLRVTDCS QLLREWPKE VVLRDDVCS AQCGFLLRN EGPQPGLNT DLTPPPRHL RARQLGRPP RIPSRGFNG FGEFKLPDL SVFVAK
- >TC005493 RA
atggaggtg acgtttgga ttagccctc ctggcgctc ctccccgcc gccctctcc gccaccaac atcaccgcc tacacgcgc gccctccct tccgaactc aaaccaacg gtgagggcg gccctgggg gcggagctg aacgcgccc cgtccttgc caatcgcac gatgccgtc ccgtgtccg cccaataaa taccggcgg ccgtcgggc gaatgtaac aatgtacga cacgcccat tggggcacg cggggtgtg ccgtttttg cgactcctc ccaccggac tacgccgat gggcggagc cagccgcgc caatcggtc acgacgcac gcgctgccg agcccattg gacgtcgct gtgaccttg caaacggtg ccggccacg ccccatgag tcagtcacc gcccttttg ggggcgtgg agcgaatta ttactacat gatttggca agcactggc aatttaaag agccaggat tgttgcagc gatgggcgg aagcatggc gagtgctat gggagggtg gggcatgga cagtgtcgg gaatatatg cggacgcta cccgccatt gacatggac gactgcgat tttgagtac cgaaatcaa atgaatttg gcttcgtcc tttttggat gcgtcggca atttacgga aataccgac caacaagtc gaaaaatta cgaacgtac gacgcgggt ctcgtcaat gtctcggcg tgcacctcg tgtcgctcc aacgcactc tattcggcc attttgaag gagcacaat cgagttgcc attaactta gcccagttg aatcgccac tggactgac gaaactttg ttcctagag agtaaacga atcgtgacg gccatgcta cagcacatc acctacaac gagtttctc cccattgta ttgggcaat gaagccatc gtacagtcc gacctccaa ttgcaaagt catgggcgg ttctctaaa tactcgagc tcgcacagg gccggtgtc tacaatgag gtggccatg agcgctctc cccgccctc ctgtcaatg atccccacc actttgatg aacgaaaca gttgaaagt ttcgccgaa atggtggac gtcttgatc agaaccccg gcccaaagc cccagtatg cacatgatg gtaccacta cgtgcggat tgggacacg gcggccctt ttcgtccat atgggccgc gaccatggg attcccccc tacgcccat tatctctcc aagtgccaa aacctcaca attgagaaa ttcgacgac tttaccaag gctggaatc agccccgaa ctgctcaaa gccttgaag tacctctac caagtacca gaggacgta gaccttttg gttggttcc ttgctggaa cgcccaatt ccgggggca atcgttggc ggaaccctt gaatgtctc ctccgtgag caatttatt cttctgaag caaagcgac cgcttctgg tacgaaaat gacctacct ccaagttcg ctaacaacg gaacaactt gccgaaatc aagaaggtc acccttgct gggttattg tgcgcaaac accgacgat ttggacaag atccaacct aaagccttc gtccaggag gatccgtac ttgaacgcc cgcttgtcc tgcgaccaa caccccctc ccccagctc accccctgg ctggagatg gaccacatg gccgacctc tccgaagac ctgctcatg gaagccctc gtcaaagcc gagcaagag gtcctagag cgtcgcaaa accgaatac gaactgtgg gccaccgtt ggcggaatc gaccccaag tcacctgca gggaccgcc gcctccttc agcaaggcc aacaaacag gccctcaaa ctcgcaaac acgtctcta ttgtttgaa ttcgcctca aacgaaatc atcaattct ttaatgcac cggaggcgc aaacgccaa attttcgac aataacgtt atttcatcc gtctttaga gacgaacta tcggatgga ttacaaaac gttgatatt tcgtctcta atcccggcc aacgctatt gatttggac acacattgt gaggagacc ggtccttgc gacccgaac acgccttat aggacgatg actggtcat tgtaacaat ttgcggaac cctaattgg ggcaaaagt ctgactacg ttcagtcgg ctcctccca agcgcctac gacgacgga atatctaag ccacgcctc ttgggcgcg accggcgtg cctttgccc tccccccgc atcgtctcc accctcatc caccccgac atcagcaac ctccactcc cgttacacc ctcatgatc atgcaatat ggccaattc ctggaccat gacttgacc atgacgccc atccataag ggtttccac gagtctatc ccggattgc cggtcatgt gacagtccc cggaccgtt cacccggaa tgtaaccct ttccccatc ccgccctct gatcactac tacccggaa attaacata acgtccggt caaagaatg tgcttcccg tttatgagg tccctccct ggacaactc cacctaggg ccacgggaa caagtcaat caaaacaca gccttcttg gacgcgtcc caaatctac ggggaaaac ccgtgcgtt ttgaaagaa ttgaaaggt tacggtggg cgtatgaac tgcacgcaa cgccccctc aaactcaag gacctcctc ccgcaaagt gaccaccac ccagaatgc aaggctggc agtgggttg tgtttcatc gcaggcgat gggcgggcc tcagaacag cccggttta accgtaatt cacacgatt ttcatgcgg gaacataac cggatggtg gaagggtta aaacaagtg aaccctcac tgggacgac cagaagtta ttcgaacac gcgcgccgt attaacata gccgccaac caacatata acttacaac gaatggtta cctcgcatt ttgagctgg aatgctgtt aacttgtac ggcttgaag ctcctcccc cagggttac tacaaggat tacaacccg tcgtgcaac cccgccatt ttgactgaa ttcgcagcg gcggcgttc cgcattggt cactcgtta ctccgcccc catatcccc cgtctaagc ccctcctac cagatcatc gacccgccc attctcctc cgtgacggt ttcttcaaa cccgatatg ctcctccaa accggaatg gtggacgaa atcgcccga ggcctcgtc tccacacca atggaaaca ctcgaccaa ttcataacc ggggaagtg accaatcac ctgttcgaa gatcggaaa atccccttc tcgggggtg gacctaatc gccctcaac atccaacgc gcgcgcgac cacggaatc ccctcctac aacaactac cgcgcctta tgcaacctc aaacgcgcc tcaaacttt gaggacctg gccagggaa atccccccc gaagtgatc gctcgcttc aagcgaatt tacccgacc gtcgacgac atcgacctg ttccccggc ggtttaagc gaacgcccg cttcaagga ggcctcgtc ggccccacc ttcgcctgc atcatagca atccagttc cgccaactg cgaaaatgc gaccgcttc tggtacgaa aaccacgac ccgactgtt cgtttctcc gaagcacaa ctcgcggaa atccgtaaa gtcaccctc gcgaaaatc atctgtgat aatctagac gtccatggc gacatgcag cgcgccgcc tttgacctc cccagcaat ttcctcaat ccgcgagtg ccgtgcgac tccctcccg aagctcgat ttcggggcg tggcgcgag tcggtgggg tcggggtgt gtcattggc gacaggatg gtagaagtg ggccaatcg gcgttcccc acgccgtgc acgagctgt atttgcacg caggagggc gggcaatgt gcgtcgttg cgggtcacc gattgctcg cagcttttg agggagtgg ccaaaggag gtggtgcta agggatgat gtgtgttcg gcccaatgc gggttcttg ttgcggaat gagggcccg cagccgggg ctgaacacc gatttgacg ccgcccccg aggcatctg agggcgcgg cagttggga cgaccgccc aggatcccg agtaggggg tttaatggg ttcggggag tttaaactg cccgatttg tcggtgttt gtggccaag tga
- >TC005493 RA
taagctcct cgtggattt ccagagagc acaagagtt cgcgttgtg gaacagacg agatctgtt tgttggttg ttgtcttcc gagcgtgag cgattatgt gtgtgcact tatcttggt tttgaactt ccctttctg tgttttttt tctgcgaca tggaggtga cgtttggat tagccctcc tggcgctcc tccccgccg ccctctccg ccaccaaca tcaccgcct acacgcgcg ccctccctt ccgaactca aaccaacgg tgagggcgg ccctggggg cggagctga acgcgcccc gtccttgcc aatcgcacg atgccgtcc cgtgtccgc ccaataaat accggcggc cgtcgggcg aatgtaaca atgtacgac acgcccatt ggggcacgc ggggtgtgc cgtttttgc gactcctcc caccggact acgccgatg ggcggagcc agccgcgcc aatcggtca cgacgcacg cgctgccga gcccattgg acgtcgctg tgaccttgc aaacggtgc cggccacgc cccatgagt cagtcaccg cccttttgg gggcgtgga gcgaattat tactacatg atttggcaa gcactggca atttaaaga gccaggatt gttgcagcg atgggcgga agcatggcg agtgctatg ggagggtgg ggcatggac agtgtcggg aatatatgc ggacgctac ccgccattg acatggacg actgcgatt ttgagtacc gaaatcaaa tgaatttgg cttcgtcct ttttggatg cgtcggcaa tttacggaa ataccgacc aacaagtcg aaaaattac gaacgtacg acgcgggtc tcgtcaatg tctcggcgt gcacctcgt gtcgctcca acgcactct attcggcca ttttgaagg agcacaatc gagttgcca ttaacttag cccagttga atcgccact ggactgacg aaactttgt tcctagaga gtaaacgaa tcgtgacgg ccatgctac agcacatca cctacaacg agtttctcc ccattgtat tgggcaatg aagccatcg tacagtccg acctccaat tgcaaagtc atgggcggt tctctaaat actcgagct cgcacaggg ccggtgtct acaatgagg tggccatga gcgctctcc ccgccctcc tgtcaatga tccccacca ctttgatga acgaaacag ttgaaagtt tcgccgaaa tggtggacg tcttgatca gaaccccgg cccaaagcc ccagtatgc acatgatgg taccactac gtgcggatt gggacacgg cggcccttt tcgtccata tgggccgcg accatggga ttcccccct acgcccatt atctctcca agtgccaaa acctcacaa ttgagaaat tcgacgact ttaccaagg ctggaatca gccccgaac tgctcaaag ccttgaagt acctctacc aagtaccag aggacgtag accttttgg ttggttcct tgctggaac gcccaattc cgggggcaa tcgttggcg gaacccttg aatgtctcc tccgtgagc aatttattc ttctgaagc aaagcgacc gcttctggt acgaaaatg acctacctc caagttcgc taacaacgg aacaacttg ccgaaatca agaaggtca cccttgctg ggttattgt gcgcaaaca ccgacgatt tggacaaga tccaaccta aagccttcg tccaggagg atccgtact tgaacgccc gcttgtcct gcgaccaac accccctcc cccagctca ccccctggc tggagatgg accacatgg ccgacctct ccgaagacc tgctcatgg aagccctcg tcaaagccg agcaagagg tcctagagc gtcgcaaaa ccgaatacg aactgtggg ccaccgttg gcggaatcg accccaagt cacctgcag ggaccgccg cctccttca gcaaggcca acaaacagg ccctcaaac tcgcaaaca cgtctctat tgtttgaat tcgcctcaa acgaaatca tcaattctt taatgcacc ggaggcgca aacgccaaa ttttcgaca ataacgtta tttcatccg tctttagag acgaactat cggatggat tacaaaacg ttgatattt cgtctctaa tcccggcca acgctattg atttggaca cacattgtg aggagaccg gtccttgcg acccgaaca cgccttata ggacgatga ctggtcatt gtaacaatt tgcggaacc ctaattggg gcaaaagtc tgactacgt tcagtcggc tcctcccaa gcgcctacg acgacggaa tatctaagc cacgcctct tgggcgcga ccggcgtgc ctttgccct ccccccgca tcgtctcca ccctcatcc accccgaca tcagcaacc tccactccc gttacaccc tcatgatca tgcaatatg gccaattcc tggaccatg acttgacca tgacgccca tccataagg gtttccacg agtctatcc cggattgcc ggtcatgtg acagtcccc ggaccgttc acccggaat gtaaccctt tccccatcc cgccctctg atcactact acccggaaa ttaacataa cgtccggtc aaagaatgt gcttcccgt ttatgaggt ccctccctg gacaactcc acctagggc cacgggaac aagtcaatc aaaacacag ccttcttgg acgcgtccc aaatctacg gggaaaacc cgtgcgttt tgaaagaat tgaaaggtt acggtgggc gtatgaact gcacgcaac gccccctca aactcaagg acctcctcc cgcaaagtg accaccacc cagaatgca aggctggca gtgggttgt gtttcatcg caggcgatg ggcgggcct cagaacagc ccggtttaa ccgtaattc acacgattt tcatgcggg aacataacc ggatggtgg aagggttaa aacaagtga accctcact gggacgacc agaagttat tcgaacacg cgcgccgta ttaacatag ccgccaacc aacatataa cttacaacg aatggttac ctcgcattt tgagctgga atgctgtta acttgtacg gcttgaagc tcctccccc agggttact acaaggatt acaacccgt cgtgcaacc ccgccattt tgactgaat tcgcagcgg cggcgttcc gcattggtc actcgttac tccgccccc atatccccc gtctaagcc cctcctacc agatcatcg acccgccca ttctcctcc gtgacggtt tcttcaaac ccgatatgc tcctccaaa ccggaatgg tggacgaaa tcgcccgag gcctcgtct ccacaccaa tggaaacac tcgaccaat tcataaccg gggaagtga ccaatcacc tgttcgaag atcggaaaa tccccttct cgggggtgg acctaatcg ccctcaaca tccaacgcg cgcgcgacc acggaatcc cctcctaca acaactacc gcgccttat gcaacctca aacgcgcct caaactttg aggacctgg ccagggaaa tcccccccg aagtgatcg ctcgcttca agcgaattt acccgaccg tcgacgaca tcgacctgt tccccggcg gtttaagcg aacgcccgc ttcaaggag gcctcgtcg gccccacct tcgcctgca tcatagcaa tccagttcc gccaactgc gaaaatgcg accgcttct ggtacgaaa accacgacc cgactgttc gtttctccg aagcacaac tcgcggaaa tccgtaaag tcaccctcg cgaaaatca tctgtgata atctagacg tccatggcg acatgcagc gcgccgcct ttgacctcc ccagcaatt tcctcaatc cgcgagtgc cgtgcgact ccctcccga agctcgatt tcggggcgt ggcgcgagt cggtggggt cggggtgtg tcattggcg acaggatgg tagaagtgg gccaatcgg cgttcccca cgccgtgca cgagctgta tttgcacgc aggagggcg ggcaatgtg cgtcgttgc gggtcaccg attgctcgc agcttttga gggagtggc caaaggagg tggtgctaa gggatgatg tgtgttcgg cccaatgcg ggttcttgt tgcggaatg agggcccgc agccggggc tgaacaccg atttgacgc cgcccccga ggcatctga gggcgcggc agttgggac gaccgccca ggatcccga gtagggggt ttaatgggt tcggggagt ttaaactgc ccgatttgt cggtgtttg tggccaagt gagcgaaag tggacgtta tttgtggat ttttttcga tgaacgagg gactaaaag gggtggtaa agcctcggg gaggttatt tataaaaat ttgattgtg attcgagaa gagagagaa tgttaccaa ctttagggt taatctaat ttgtgtctt ctttttgta ctttatttt attgttttt atacagtgt acactgcct tctgtatta ttattctgt tgttaatac ttgtactcc tctgactat gcattatta aactaccat tattttttt attccgtgt ccagcttac caataaaaa cctacaatt ttt
