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Gene information
General information
TC005480 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 884052..889524
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005480
    iBeetle screen iB_00865
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005480 RA
      MRQKVNDFH WCWARSGEG QLCRVFLAE DTPCHVLLE 
      LTPKSPLVK IDSNCCAIL PVFGTMLPI WTISLLLFT 
      STFAMDNLR VAYQWKELD FDFPSASAR EEAIKSGKF 
      IPENNLPLG LEVYKDRLF ITVPRWRSG VAASLNYIK 
      LSDPMDSPK LKPYPNWEA HKSHSGDPP EIVSPFRVR 
      ADHCGRLWV LDTGIEDFT NPKTHKPVT LLIYDLHND 
      NLMRSFKIP KGQVKSESF LANIAVEDH NCADTYAYL 
      GDLGDPPGI IVYSWKKGE SWRVEHNYF SIDPLMGEF 
      NVSGITFVW NDGLFGMAL SKPDHDGFS TLYFHPMSS 
      TNEFSVSTK YLREDSSKE HFHEFKLLG TRGPKGQSG 
      VSFLDQKTD VLFYSLLNL NAVACWRTT NPAYTMESQ 
      GRVYMNNVT MIFPNDIKV DNDDNLWVL SDRLPMFLY 
      SHLDKNDVN FRVLTAPVA DAIRGTACD SKLVTRIKS 
      NDLKNGGLR KSAVGGVVL VMFVAIMNL
    CDS sequence
    • >TC005480 RA
      atgcggcaa aaggtaaat gactttcac tggtgttgg 
      gcaagaagt ggcgaaggt cagttgtgc cgcgtattc 
      ctagccgaa gacacacct tgccatgtg ctcctagag 
      ctcactccg aaaagtcca cttgttaaa atcgactcg 
      aattgttgt gccattttg cccgttttt ggtacgatg 
      cttccaata tggacaatt tccctcctc ctctttacc 
      tcaaccttc gccatggac aaccttcgt gtcgcctat 
      caatggaaa gagctcgac tttgacttc ccctcggcc 
      tctgcgcgt gaagaggcc atcaaaagc ggaaaattc 
      attccggaa aataacctg cctctaggt ctagaagtc 
      tacaaggac cggcttttc atcacggtg ccgagatgg 
      cgctcgggg gtcgccgcc tccctcaac tacatcaag 
      ctttcagac cccatggac tcgcccaaa ctcaaaccc 
      taccccaac tgggaggcg cacaagagc cactctgga 
      gacccccca gagatagtc tccccattt cgtgtccga 
      gccgaccat tgtggtcgc ttatgggtc ttggacacc 
      ggtatcgaa gacttcaca aaccccaaa acccacaaa 
      cctgtcact ttgctaatt tacgacctc cacaacgat 
      aacctaatg cggagtttc aaaatcccc aaaggccaa 
      gtcaagagt gagagtttt ctggccaac atcgccgtg 
      gaggaccac aactgtgcc gacacttat gcgtattta 
      ggtgacctg ggggaccct ccaggcatt atcgtgtat 
      tcgtggaaa aagggggag agctggagg gtggagcat 
      aattatttc tccattgac ccattgatg ggagagttc 
      aacgtttct gggatcacg ttcgtgtgg aacgatggg 
      ttgttcggg atggccttg tccaagccg gaccatgat 
      gggtttagc acgctttac ttccaccca atgagcagc 
      accaacgag ttctcggtc agcaccaag tacctgcgg 
      gaggactcc tccaaggag catttccac gagttcaag 
      ttgctgggc actaggggg cccaagggg caaagtggg 
      gtctcgttc ctcgaccag aagaccgac gttctgttc 
      tattctttg ttgaatttg aacgctgtc gcgtgttgg 
      agaacaaca aaccctgct tataccatg gagtcgcaa 
      gggagggtt tacatgaac aacgtcacc atgattttc 
      cccaatgat atcaaagtt gataatgat gataatttg 
      tgggttttg tcggatcgg ctgccgatg tttttgtac 
      agtcatttg gataagaat gatgttaat tttcgggtg 
      ctgacggca ccggttgct gatgcgatc agaggaacg 
      gcgtgcgat tcgaaattg gtgacgagg atcaagagt 
      aatgatttg aagaatggt ggtttgaga aaaagtgct 
      gtgggaggg gtggttctt gtcatgttt gttgccata 
      atgaatttg taa
    mRNA sequence
    • >TC005480 RA
      gacagaaat aagaaaata atgcggcaa aaggtaaat 
      gactttcac tggtgttgg gcaagaagt ggcgaaggt 
      cagttgtgc cgcgtattc ctagccgaa gacacacct 
      tgccatgtg ctcctagag ctcactccg aaaagtcca 
      cttgttaaa atcgactcg aattgttgt gccattttg 
      cccgttttt ggtacgatg cttccaata tggacaatt 
      tccctcctc ctctttacc tcaaccttc gccatggac 
      aaccttcgt gtcgcctat caatggaaa gagctcgac 
      tttgacttc ccctcggcc tctgcgcgt gaagaggcc 
      atcaaaagc ggaaaattc attccggaa aataacctg 
      cctctaggt ctagaagtc tacaaggac cggcttttc 
      atcacggtg ccgagatgg cgctcgggg gtcgccgcc 
      tccctcaac tacatcaag ctttcagac cccatggac 
      tcgcccaaa ctcaaaccc taccccaac tgggaggcg 
      cacaagagc cactctgga gacccccca gagatagtc 
      tccccattt cgtgtccga gccgaccat tgtggtcgc 
      ttatgggtc ttggacacc ggtatcgaa gacttcaca 
      aaccccaaa acccacaaa cctgtcact ttgctaatt 
      tacgacctc cacaacgat aacctaatg cggagtttc 
      aaaatcccc aaaggccaa gtcaagagt gagagtttt 
      ctggccaac atcgccgtg gaggaccac aactgtgcc 
      gacacttat gcgtattta ggtgacctg ggggaccct 
      ccaggcatt atcgtgtat tcgtggaaa aagggggag 
      agctggagg gtggagcat aattatttc tccattgac 
      ccattgatg ggagagttc aacgtttct gggatcacg 
      ttcgtgtgg aacgatggg ttgttcggg atggccttg 
      tccaagccg gaccatgat gggtttagc acgctttac 
      ttccaccca atgagcagc accaacgag ttctcggtc 
      agcaccaag tacctgcgg gaggactcc tccaaggag 
      catttccac gagttcaag ttgctgggc actaggggg 
      cccaagggg caaagtggg gtctcgttc ctcgaccag 
      aagaccgac gttctgttc tattctttg ttgaatttg 
      aacgctgtc gcgtgttgg agaacaaca aaccctgct 
      tataccatg gagtcgcaa gggagggtt tacatgaac 
      aacgtcacc atgattttc cccaatgat atcaaagtt 
      gataatgat gataatttg tgggttttg tcggatcgg 
      ctgccgatg tttttgtac agtcatttg gataagaat 
      gatgttaat tttcgggtg ctgacggca ccggttgct 
      gatgcgatc agaggaacg gcgtgcgat tcgaaattg 
      gtgacgagg atcaagagt aatgatttg aagaatggt 
      ggtttgaga aaaagtgct gtgggaggg gtggttctt 
      gtcatgttt gttgccata atgaatttg taattttta 
      caaacgtcc tgtaattat tgtataatt gccgcgatt 
      tatttattt attttagga tagtttgta gtgtcttgt 
      ttgtaagta ttgtactta tttgtaaaa ataaagtca 
      tcaagagtt t