Status
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Gene information
General information
TC005477 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12497780..12509459
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005477
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00864

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005477 RA
      MESIGDYEY NTKDIIGHG AFAVVFKGR ERKNPSNTV 
      AIKCITKKN IAKQSLLGK EIKILQELT ELHHENVVA 
      LFDCKESPN NVYLVMEYC KGGDLADYL AAKGTLSED 
      TIRLFLIQL AGAMKALSA KGIVHRDLK PQNILITYD 
      VPNPQPSQI KLKIADFGF ARFLQEGVM AATLCGSPM 
      YMAPEVILA LQYDAKADL WSLGTIVFQ CLTGKAPFQ 
      ANTPQALKQ IYDTTMNLV PKIPHGTSP ELTDLLLGL 
      LKRNAKERM NFDQFFNHK FLKRAETPK ASSPLQNDM 
      PLPIGTPPN ITAVPSSSP RPVRGNSIS QPDPPESPR 
      STRTALSSS PEDDYVIVS RNIPDQSTE SVELEKQFK 
      MTTVRSPPA PCVPVASPV RPSFLPVSE PLPVPTMKD 
      VYKQTSEKK DDHSPIKPT VNSNVPRSQ PISMKRSEN 
      RPIHNDIDI SSLSPPSVK FVIGTPPGG RRRSTSGGS 
      LCETPPPTN FWTPSPVAK TAPVNSPLR RSGTSPPMQ 
      SGQLARMPI LSSPNLNNE NNNPNRSPN MPYCTRAVT 
      LPEISEIRN YAIFNDSYE HPITFLAPE LPEETLLEK 
      EHNETLAKI NYVLAVCTC ILDLAAVRS TPIGALHDS 
      VQLQSQIQN ELMKRAERF VLLVRALQL LGSGLALAT 
      NQMKSGQLR PSSKVKNVM SSLNSRFRS TLAECKQLN 
      SPELMAKVA DITADKLLY DHAVQICQA AALDELFDN 
      PQQCFERYQ TAQILLHSL SQQLPQQDR ALLTKYKEA 
      VEKRLFMLQ QRGYIYSTD LSQIN
    • >TC005477 RB
      MKYEYCKGG DLADYLAAK GTLSEDTIR LFLIQLAGA 
      MKALSAKGI VHRDLKPQN ILITYDVPN PQPSQIKLK 
      IADFGFARF LQEGVMAAT LCGSPMYMA PEVILALQY 
      DAKADLWSL GTIVFQCLT GKAPFQANT PQALKQIYD 
      TTMNLVPKI PHGTSPELT DLLLGLLKR NAKERMNFD 
      QFFNHKFLK RAETPKASS PLQNDMPLP IGTPPNITA 
      VPSSSPRPV RGNSISQPD PPESPRSTR TALSSSPED 
      DYVIVSRNI PDQSTESVE LEKQFKMTT VRSPPAPCV 
      PVASPVRPS FLPVSEPLP VPTMKDVYK QTSEKKDDH 
      SPIKPTVNS NVPRSQPIS MKRSENRPI HNDIDISSL 
      SPPSVKFVI GTPPGGRRR STSGGSLCE TPPPTNFWT 
      PSPVAKTAP VNSPLRRSG TSPPMQSGQ LARMPILSS 
      PNLNNENNN PNRSPNMPY CTRAVTLPE ISEIRNYAI 
      FNDSYEHPI TFLAPELPE ETLLEKEHN ETLAKINYV 
      LAVCTCILD LAAVRSTPI GALHDSVQL QSQIQNELM 
      KRAERFVLL VRALQLLGS GLALATNQM KSGQLRPSS 
      KVKNVMSSL NSRFRSTLA ECKQLNSPE LMAKVADIT 
      ADKLLYDHA VQICQAAAL DELFDNPQQ CFERYQTAQ 
      ILLHSLSQQ LPQQDRALL TKYKEAVEK RLFMLQQRG 
      YIYSTDLSQ IN
    CDS sequence
    • >TC005477 RA
      atggaaagt atcggcgat tacgagtac aatactaag 
      gatataata ggccatggg gcctttgcc gtggttttc 
      aagggccgg gagaggaag aacccgtcg aataccgtt 
      gctatcaag tgcataact aagaaaaat atagcgaaa 
      caaagcttg ttagggaag gagataaag attttgcag 
      gagctgact gagctgcac catgagaat gttgtggct 
      ttgtttgac tgcaaggag tctcctaat aatgtatac 
      ttagtaatg gagtactgt aaaggcggc gatttggcc 
      gattacctc gctgctaag ggcaccctt agcgaggac 
      acaattcgc ctatttctg atccaatta gctggagca 
      atgaaagct ctctcggcc aaaggcatc gtccaccgt 
      gacctcaaa cctcaaaac atcctcatc acttacgac 
      gtccctaat cctcaacca tcgcaaatt aagctgaaa 
      atcgccgac tttggtttc gcccgattt ttacaagag 
      ggcgtaatg gcggccact ttatgtggc agccctatg 
      tatatggcc cccgaagtg attctggcc cttcaatac 
      gacgccaaa gccgatctt tggagtctc ggaacgatc 
      gtttttcag tgtctgact ggcaaagca ccgtttcag 
      gccaataca ccgcaagca ctcaaacaa atttatgac 
      accaccatg aatctagtc cccaagatc ccgcatggg 
      actagtcca gaattgacc gatcttttg ctcggtttg 
      ctgaaacgt aacgctaag gaacgaatg aattttgac 
      cagtttttc aatcacaag tttttgaag agggctgag 
      acgcccaaa gcgagtagt ccgttacaa aatgatatg 
      ccgttacca atcgggact cccccgaat attacagcc 
      gtaccatca agtagccct cgaccagtc cgaggcaac 
      tcgatttcg caacccgat cctccagaa tctccaaga 
      tctacaaga acggctctt agttcgagt cctgaagat 
      gattatgtt atagtgtcg agaaatatc ccggaccag 
      tcgaccgaa agcgtcgag ttggagaaa cagttcaag 
      atgacgact gttaggtcc ccacctgca ccttgtgtc 
      cctgttgca agccccgtc cggccttcc ttcctccct 
      gtttctgag cccctccct gtccctacc atgaaagac 
      gtctacaag caaacaagc gaaaaaaaa gatgaccac 
      agcccaatt aaaccgact gtcaatagc aacgtcccc 
      cgatcacaa ccgatcagc atgaaacgg tccgaaaat 
      cgaccgatt cacaacgat atcgatatc agttcccta 
      tcacctcct tcagtaaaa ttcgttatc gggacacca 
      cctggtgga cgacgacgt tccacttcc ggtggaagt 
      ctctgtgaa acgccacca ccgacgaat ttctggacg 
      ccgtcaccg gtggccaaa acagcaccg gttaattcg 
      ccgttaagg cggtccggt acttccccg cccatgcaa 
      agtggacag ttggcacga atgccaatt ttgagtagt 
      ccgaattta aataatgaa aataataat ccgaatagg 
      tcaccaaat atgccctat tgtacaagg gcggtcact 
      ttaccggaa atttccgaa attcggaat tatgccatt 
      tttaacgat agttatgaa catcccatt acgtttctg 
      gcgccggag ttgcccgaa gagacgctt cttgagaag 
      gaacacaac gaaaccttg gcgaaaatt aactacgtg 
      ctggccgtg tgcacgtgc atcctcgac ttggcggcg 
      gtccgttcg acgccaatc ggcgccctt cacgactca 
      gtccagctc caatcgcaa attcaaaac gagctgatg 
      aaaagagcc gaacgtttt gtcctttta gttcgtgct 
      ttacaattg ttaggttcc ggtctggcg ttagcaacg 
      aaccaaatg aaatccggt caattacga ccgagctca 
      aaggtaaaa aacgtcatg tcgagtctc aatagcaga 
      ttccggtcg acattggcc gagtgcaaa caattgaac 
      agtcctgaa cttatggcc aaagtggcc gatattaca 
      gccgataaa ctgctatac gatcatgcc gtgcaaatt 
      tgtcaagcg gccgccttg gatgaacta ttcgataat 
      ccgcaacag tgtttcgag agatatcaa acggcgcaa 
      attcttttg cacagttta tcgcaacag ttgccccaa 
      caagatcgg gcattgctt acgaaatat aaggaggcc 
      gtggagaag cgcctgttc atgctgcag cagcggggc 
      tacatctat tcgacggat ctcagtcaa ataaattaa
    • >TC005477 RB
      atgaaatat gaatactgt aaaggcggc gatttggcc 
      gattacctc gctgctaag ggcaccctt agcgaggac 
      acaattcgc ctatttctg atccaatta gctggagca 
      atgaaagct ctctcggcc aaaggcatc gtccaccgt 
      gacctcaaa cctcaaaac atcctcatc acttacgac 
      gtccctaat cctcaacca tcgcaaatt aagctgaaa 
      atcgccgac tttggtttc gcccgattt ttacaagag 
      ggcgtaatg gcggccact ttatgtggc agccctatg 
      tatatggcc cccgaagtg attctggcc cttcaatac 
      gacgccaaa gccgatctt tggagtctc ggaacgatc 
      gtttttcag tgtctgact ggcaaagca ccgtttcag 
      gccaataca ccgcaagca ctcaaacaa atttatgac 
      accaccatg aatctagtc cccaagatc ccgcatggg 
      actagtcca gaattgacc gatcttttg ctcggtttg 
      ctgaaacgt aacgctaag gaacgaatg aattttgac 
      cagtttttc aatcacaag tttttgaag agggctgag 
      acgcccaaa gcgagtagt ccgttacaa aatgatatg 
      ccgttacca atcgggact cccccgaat attacagcc 
      gtaccatca agtagccct cgaccagtc cgaggcaac 
      tcgatttcg caacccgat cctccagaa tctccaaga 
      tctacaaga acggctctt agttcgagt cctgaagat 
      gattatgtt atagtgtcg agaaatatc ccggaccag 
      tcgaccgaa agcgtcgag ttggagaaa cagttcaag 
      atgacgact gttaggtcc ccacctgca ccttgtgtc 
      cctgttgca agccccgtc cggccttcc ttcctccct 
      gtttctgag cccctccct gtccctacc atgaaagac 
      gtctacaag caaacaagc gaaaaaaaa gatgaccac 
      agcccaatt aaaccgact gtcaatagc aacgtcccc 
      cgatcacaa ccgatcagc atgaaacgg tccgaaaat 
      cgaccgatt cacaacgat atcgatatc agttcccta 
      tcacctcct tcagtaaaa ttcgttatc gggacacca 
      cctggtgga cgacgacgt tccacttcc ggtggaagt 
      ctctgtgaa acgccacca ccgacgaat ttctggacg 
      ccgtcaccg gtggccaaa acagcaccg gttaattcg 
      ccgttaagg cggtccggt acttccccg cccatgcaa 
      agtggacag ttggcacga atgccaatt ttgagtagt 
      ccgaattta aataatgaa aataataat ccgaatagg 
      tcaccaaat atgccctat tgtacaagg gcggtcact 
      ttaccggaa atttccgaa attcggaat tatgccatt 
      tttaacgat agttatgaa catcccatt acgtttctg 
      gcgccggag ttgcccgaa gagacgctt cttgagaag 
      gaacacaac gaaaccttg gcgaaaatt aactacgtg 
      ctggccgtg tgcacgtgc atcctcgac ttggcggcg 
      gtccgttcg acgccaatc ggcgccctt cacgactca 
      gtccagctc caatcgcaa attcaaaac gagctgatg 
      aaaagagcc gaacgtttt gtcctttta gttcgtgct 
      ttacaattg ttaggttcc ggtctggcg ttagcaacg 
      aaccaaatg aaatccggt caattacga ccgagctca 
      aaggtaaaa aacgtcatg tcgagtctc aatagcaga 
      ttccggtcg acattggcc gagtgcaaa caattgaac 
      agtcctgaa cttatggcc aaagtggcc gatattaca 
      gccgataaa ctgctatac gatcatgcc gtgcaaatt 
      tgtcaagcg gccgccttg gatgaacta ttcgataat 
      ccgcaacag tgtttcgag agatatcaa acggcgcaa 
      attcttttg cacagttta tcgcaacag ttgccccaa 
      caagatcgg gcattgctt acgaaatat aaggaggcc 
      gtggagaag cgcctgttc atgctgcag cagcggggc 
      tacatctat tcgacggat ctcagtcaa ataaattaa
    mRNA sequence
    • >TC005477 RA
      gatggcgcc ccgtcattg acatattgc gattcagac 
      aaaaacaag tcgtttttt cgcaattgt gcgataacg 
      tgactaaat tcagtgttt ttgcgctta attccactt 
      tttcatcat gtaggacgt gcaagaatt gattacaga 
      aacagtttt taccacctt tgataagtt gtaatctgt 
      tgcactttt tgcccaaaa atggaaagt atcggcgat 
      tacgagtac aatactaag gatataata ggccatggg 
      gcctttgcc gtggttttc aagggccgg gagaggaag 
      aacccgtcg aataccgtt gctatcaag tgcataact 
      aagaaaaat atagcgaaa caaagcttg ttagggaag 
      gagataaag attttgcag gagctgact gagctgcac 
      catgagaat gttgtggct ttgtttgac tgcaaggag 
      tctcctaat aatgtatac ttagtaatg gagtactgt 
      aaaggcggc gatttggcc gattacctc gctgctaag 
      ggcaccctt agcgaggac acaattcgc ctatttctg 
      atccaatta gctggagca atgaaagct ctctcggcc 
      aaaggcatc gtccaccgt gacctcaaa cctcaaaac 
      atcctcatc acttacgac gtccctaat cctcaacca 
      tcgcaaatt aagctgaaa atcgccgac tttggtttc 
      gcccgattt ttacaagag ggcgtaatg gcggccact 
      ttatgtggc agccctatg tatatggcc cccgaagtg 
      attctggcc cttcaatac gacgccaaa gccgatctt 
      tggagtctc ggaacgatc gtttttcag tgtctgact 
      ggcaaagca ccgtttcag gccaataca ccgcaagca 
      ctcaaacaa atttatgac accaccatg aatctagtc 
      cccaagatc ccgcatggg actagtcca gaattgacc 
      gatcttttg ctcggtttg ctgaaacgt aacgctaag 
      gaacgaatg aattttgac cagtttttc aatcacaag 
      tttttgaag agggctgag acgcccaaa gcgagtagt 
      ccgttacaa aatgatatg ccgttacca atcgggact 
      cccccgaat attacagcc gtaccatca agtagccct 
      cgaccagtc cgaggcaac tcgatttcg caacccgat 
      cctccagaa tctccaaga tctacaaga acggctctt 
      agttcgagt cctgaagat gattatgtt atagtgtcg 
      agaaatatc ccggaccag tcgaccgaa agcgtcgag 
      ttggagaaa cagttcaag atgacgact gttaggtcc 
      ccacctgca ccttgtgtc cctgttgca agccccgtc 
      cggccttcc ttcctccct gtttctgag cccctccct 
      gtccctacc atgaaagac gtctacaag caaacaagc 
      gaaaaaaaa gatgaccac agcccaatt aaaccgact 
      gtcaatagc aacgtcccc cgatcacaa ccgatcagc 
      atgaaacgg tccgaaaat cgaccgatt cacaacgat 
      atcgatatc agttcccta tcacctcct tcagtaaaa 
      ttcgttatc gggacacca cctggtgga cgacgacgt 
      tccacttcc ggtggaagt ctctgtgaa acgccacca 
      ccgacgaat ttctggacg ccgtcaccg gtggccaaa 
      acagcaccg gttaattcg ccgttaagg cggtccggt 
      acttccccg cccatgcaa agtggacag ttggcacga 
      atgccaatt ttgagtagt ccgaattta aataatgaa 
      aataataat ccgaatagg tcaccaaat atgccctat 
      tgtacaagg gcggtcact ttaccggaa atttccgaa 
      attcggaat tatgccatt tttaacgat agttatgaa 
      catcccatt acgtttctg gcgccggag ttgcccgaa 
      gagacgctt cttgagaag gaacacaac gaaaccttg 
      gcgaaaatt aactacgtg ctggccgtg tgcacgtgc 
      atcctcgac ttggcggcg gtccgttcg acgccaatc 
      ggcgccctt cacgactca gtccagctc caatcgcaa 
      attcaaaac gagctgatg aaaagagcc gaacgtttt 
      gtcctttta gttcgtgct ttacaattg ttaggttcc 
      ggtctggcg ttagcaacg aaccaaatg aaatccggt 
      caattacga ccgagctca aaggtaaaa aacgtcatg 
      tcgagtctc aatagcaga ttccggtcg acattggcc 
      gagtgcaaa caattgaac agtcctgaa cttatggcc 
      aaagtggcc gatattaca gccgataaa ctgctatac 
      gatcatgcc gtgcaaatt tgtcaagcg gccgccttg 
      gatgaacta ttcgataat ccgcaacag tgtttcgag 
      agatatcaa acggcgcaa attcttttg cacagttta 
      tcgcaacag ttgccccaa caagatcgg gcattgctt 
      acgaaatat aaggaggcc gtggagaag cgcctgttc 
      atgctgcag cagcggggc tacatctat tcgacggat 
      ctcagtcaa ataaattaa ttcacgtaa ctataattc 
      caataattc ttggcgtat tcactgtta accaatcgc 
      ttgatctag ttgctgtct tgtaccgac agtttagtt 
      gaaatatcg cgttgcgtc attgttttt cttccacca 
      tcgcataca tgacgggat gaggagagt cagtcgggt 
      cgtgcttta tgatttgtg ttttaattg tatacaaag 
      taattaaat aaaagtaat ttatttgtg gatgcggta 
      ataaatgac ccctcctag t
    • >TC005477 RB
      aataatctt gcgtttaaa taataaaat ataaacagt 
      ttattttat ttgtttcta aatgaaata tgaatactg 
      taaaggcgg cgatttggc cgattacct cgctgctaa 
      gggcaccct tagcgagga cacaattcg cctatttct 
      gatccaatt agctggagc aatgaaagc tctctcggc 
      caaaggcat cgtccaccg tgacctcaa acctcaaaa 
      catcctcat cacttacga cgtccctaa tcctcaacc 
      atcgcaaat taagctgaa aatcgccga ctttggttt 
      cgcccgatt tttacaaga gggcgtaat ggcggccac 
      tttatgtgg cagccctat gtatatggc ccccgaagt 
      gattctggc ccttcaata cgacgccaa agccgatct 
      ttggagtct cggaacgat cgtttttca gtgtctgac 
      tggcaaagc accgtttca ggccaatac accgcaagc 
      actcaaaca aatttatga caccaccat gaatctagt 
      ccccaagat cccgcatgg gactagtcc agaattgac 
      cgatctttt gctcggttt gctgaaacg taacgctaa 
      ggaacgaat gaattttga ccagttttt caatcacaa 
      gtttttgaa gagggctga gacgcccaa agcgagtag 
      tccgttaca aaatgatat gccgttacc aatcgggac 
      tcccccgaa tattacagc cgtaccatc aagtagccc 
      tcgaccagt ccgaggcaa ctcgatttc gcaacccga 
      tcctccaga atctccaag atctacaag aacggctct 
      tagttcgag tcctgaaga tgattatgt tatagtgtc 
      gagaaatat cccggacca gtcgaccga aagcgtcga 
      gttggagaa acagttcaa gatgacgac tgttaggtc 
      cccacctgc accttgtgt ccctgttgc aagccccgt 
      ccggccttc cttcctccc tgtttctga gcccctccc 
      tgtccctac catgaaaga cgtctacaa gcaaacaag 
      cgaaaaaaa agatgacca cagcccaat taaaccgac 
      tgtcaatag caacgtccc ccgatcaca accgatcag 
      catgaaacg gtccgaaaa tcgaccgat tcacaacga 
      tatcgatat cagttccct atcacctcc ttcagtaaa 
      attcgttat cgggacacc acctggtgg acgacgacg 
      ttccacttc cggtggaag tctctgtga aacgccacc 
      accgacgaa tttctggac gccgtcacc ggtggccaa 
      aacagcacc ggttaattc gccgttaag gcggtccgg 
      tacttcccc gcccatgca aagtggaca gttggcacg 
      aatgccaat tttgagtag tccgaattt aaataatga 
      aaataataa tccgaatag gtcaccaaa tatgcccta 
      ttgtacaag ggcggtcac tttaccgga aatttccga 
      aattcggaa ttatgccat ttttaacga tagttatga 
      acatcccat tacgtttct ggcgccgga gttgcccga 
      agagacgct tcttgagaa ggaacacaa cgaaacctt 
      ggcgaaaat taactacgt gctggccgt gtgcacgtg 
      catcctcga cttggcggc ggtccgttc gacgccaat 
      cggcgccct tcacgactc agtccagct ccaatcgca 
      aattcaaaa cgagctgat gaaaagagc cgaacgttt 
      tgtcctttt agttcgtgc tttacaatt gttaggttc 
      cggtctggc gttagcaac gaaccaaat gaaatccgg 
      tcaattacg accgagctc aaaggtaaa aaacgtcat 
      gtcgagtct caatagcag attccggtc gacattggc 
      cgagtgcaa acaattgaa cagtcctga acttatggc 
      caaagtggc cgatattac agccgataa actgctata 
      cgatcatgc cgtgcaaat ttgtcaagc ggccgcctt 
      ggatgaact attcgataa tccgcaaca gtgtttcga 
      gagatatca aacggcgca aattctttt gcacagttt 
      atcgcaaca gttgcccca acaagatcg ggcattgct 
      tacgaaata taaggaggc cgtggagaa gcgcctgtt 
      catgctgca gcagcgggg ctacatcta ttcgacgga 
      tctcagtca aataaatta attcacgta actataatt 
      ccaataatt cttggcgta ttcactgtt aaccaatcg 
      cttgatcta gttgctgtc ttgtaccga cagtttagt 
      tgaaatatc gcgttgcgt cattgtttt tcttccacc 
      atcgcatac atgacggga tgaggagag tcagtcggg 
      tcgtgcttt atgatttgt gttttaatt gtatacaaa 
      gtaattaaa taaaagtaa tttatttgt ggatgcggt 
      aataaatga cccctccta gt
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