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Gene information
General information
TC005459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 1337522..1357239
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005459
    iBeetle screen iB_00861
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005459 RA
      MVLITDRQK INLVNLLVT VCVVFIAVD VSSGSFATR 
      INKISENLD KALSQVIHQ TPNYTDLTD NATYVSDAS 
      FNPRGMEGL YNFTNAFME LIFSKEIYP EGFIRIENN 
      RIQIGDIEK EWKSLLKKY VGLLVMVII LALLIVFMP 
      FCGLCFCCC RCCGKCGAR SQPFDKKHD LCKKFVLAT 
      VLIGLGTCL LFGVVCAFV SNQQMEEGV GDLSQNLRK 
      GVTDIDTYL DTTKKQIET LLKKNYGEF EFVLTKMID 
      NSTAAVYGE LVNYSNAIS MTEVANIVE ALPAIEEKF 
      ITLKYSTNS LRVYASQLN DAVRKVKKD MLQILRSSD 
      CDRIAKCKE INQNISSLQ TNIHFNSLP DITDKIAKL 
      DEITKNQTL IDAVTKGKN ALEDIKVKI GDKVNATLD 
      KAHEKIQEA GDQIRHGAD NVTQVITEL KTKIDTNTK 
      PVLDTSERY IKQYSFYRY YAALAISCL LLLITIFIT 
      LGLVCGICG KRPEGYGDD CCNKGAGSQ FLICAVLFM 
      FLTGFVVAV VVLALFMVG VVTQRVVCD PMRDPANSQ 
      VFDLVDRLD FSNYGIHIT ASEALGKCY RNRSIYEVA 
      NLADKFNLA EVKNYLNSY GINQTLAEL DIGSLDILD 
      DFTLLGEKD KELLESFAV SGFAEINFD EFKDLLSTN 
      FTTVNLTDL ANEVGGLIQ ELQKYENVD QLIEDLQVS 
      KMNIEMYED KIVEPMIKN ASAVIDLAK DIERDLRLN 
      HSSFSDAMQ ALLNELEQA ENILKINGT DFLNTTVIE 
      FTTGIVNLV QTYIDRVVT KTELEVGQC GPLSIVINA 
      TLTSTCDKI LTPWNGYWF GLFWSLLLF IPTIIVSVK 
      LATLYQKHS PYSRDLVEA EYLYDAYVD RDNIPLNSR 
      NGKAKKKKK KHKRYEDRP IPSAGGDMV AREYAAGSN 
      QDARYADMA PKQWEDFPA GGPPQYQRA PTEYERPPP 
      YYYPGAGET AQ
    CDS sequence
    • >TC005459 RA
      atggtgctc atcaccgac cgccaaaaa atcaacctg 
      gtgaacctc ctggtgacc gtctgcgtc gtcttcatc 
      gccgtggac gtctcctcg gggagtttc gccactcgt 
      atcaataaa attagcgag aatttggac aaagccttg 
      tcgcaagtc attcaccaa acgccgaat tacaccgat 
      ttgacggat aatgccact tatgtcagt gatgcaagt 
      ttcaatccc cgaggaatg gaagggctg tataatttc 
      accaatgcc ttcatggag ctgattttt tcaaaggag 
      atttatccg gaaggtttt attcgaatc gaaaataac 
      cggatccag atcggagac attgaaaag gagtggaaa 
      agtctcttg aagaaatac gtgggcctt ttggtcatg 
      gtcataatt ttggctttg ctcattgtg tttatgccg 
      ttttgtggc ttgtgtttc tgctgctgc aggtgctgc 
      ggcaagtgc ggggcgagg tcgcagccc tttgacaaa 
      aaacacgac ctctgtaag aagttcgtg ctcgcgacg 
      gtgctcatt ggcctgggg acttgtctc ctgtttggc 
      gtcgtttgt gcatttgta agcaaccag cagatggag 
      gagggggtt ggcgatttg tcgcaaaat ctgcgcaaa 
      ggcgtcacc gatatcgac acttatctt gatacaacc 
      aaaaagcag atcgagact ctcctgaag aaaaattac 
      ggagagttt gagtttgtc ttgaccaaa atgattgat 
      aacagcacg gcggccgtt tatggcgaa ttggtgaat 
      tattcgaat gcgatttcg atgactgag gtcgcaaat 
      attgttgaa gcacttcct gcaattgag gaaaaattt 
      ataacgttg aagtattcg acgaattcg ttacgtgtc 
      tatgcttca cagttgaat gacgctgtt aggaaagtc 
      aaaaaagat atgttgcag attttacga tcgtctgac 
      tgcgaccga atcgccaaa tgtaaagaa atcaaccaa 
      aatattagc tcattgcaa actaacatc cacttcaac 
      agtctcccc gacataacg gacaaaatc gccaaactg 
      gacgaaatc accaaaaac caaaccctc atcgacgcc 
      gtcaccaaa ggcaagaac gccctcgaa gacatcaaa 
      gtcaaaatc ggcgataaa gtcaatgcg accttagac 
      aaagcccac gaaaaaatc caagaagcc ggcgaccaa 
      atccgccac ggcgccgac aacgtcacc caggtcatc 
      accgaactc aagacaaaa atcgacacc aacaccaaa 
      ccggtcctc gacacatcc gaacgctac ataaaacag 
      tacagtttt taccgatac tatgccgct ttggcgatc 
      agttgcttg ctgcttctg atcacgatc ttcatcacg 
      ctgggcctg gtttgcggg atctgcggc aaacggccc 
      gaaggctac ggggacgac tgttgcaat aaaggggcc 
      ggaagccaa tttctgatc tgcgctgtg ttgttcatg 
      tttctgacg gggtttgtg gtcgccgtt gtcgtcctg 
      gcgttgttc atggtcggg gtcgtgacc cagcgggtg 
      gtttgtgac ccgatgcgc gatcccgcc aactcgcaa 
      gtgttcgat ttggtcgac agattggat ttctccaac 
      tacggcatt cacataacc gccagtgag gccttgggg 
      aagtgctat cgcaacagg agcatctac gaggtggcc 
      aatctggcc gataaattc aacctggcc gaagtgaaa 
      aactacttg aactcgtat gggattaac cagactttg 
      gcagagctg gacattggg tcgctggat attctggac 
      gattttacg cttttgggc gagaaagac aaggagttg 
      ttggagtcg tttgcagtc tccggcttt gccgaaatt 
      aattttgac gagttcaaa gatttgttg agcacgaac 
      tttaccacg gttaatttg accgatctg gctaacgaa 
      gtggggggt ttgatacag gaattacaa aagtacgag 
      aacgttgat caattaatt gaagacttg caagtcagc 
      aaaatgaac attgaaatg tacgaggat aaaattgtg 
      gagccgatg atcaaaaat gcctcggct gtgatcgat 
      ctggccaag gatatagaa agagatctc aggttgaat 
      cacagctcg tttagtgac gccatgcaa gcgttgtta 
      aatgaactg gaacaggct gagaatatt ttaaaaatc 
      aatggaact gatttccta aatacgact gtcatcgaa 
      ttcaccact ggaattgtc aatcttgtc caaacttac 
      atcgaccga gtggtcact aaaaccgag ctagaggtg 
      ggtcaatgt gggcccttg agtatagtg atcaatgca 
      acgttaaca tcaacctgt gacaaaatc ctaacacct 
      tggaacggt tactggttt ggtctcttc tggtcgctc 
      cttctgttc attcccaca atcatagtt tcggttaag 
      ttggccacg ctgtaccag aaacacagt ccgtattcg 
      cgcgatctg gtcgaagcc gaatatttg tatgatgcg 
      tatgtcgat cgggacaac ataccgttg aatagcagg 
      aatgggaag gcgaagaag aagaagaag aagcacaag 
      cgttacgaa gaccggccg attccgtca gctggcggc 
      gacatggtg gcacgtgaa tacgcagcc gggtcgaac 
      caagacgcc agatatgcc gacatggca cccaagcaa 
      tgggaggac tttccggcc gggggcccc ccgcagtac 
      cagagggcg cccaccgag tacgagaga ccgcccccg 
      tattattac cccggtgct ggggaaacc gctcagtag
    mRNA sequence
    • >TC005459 RA
      aggagcgag tccgtggga ggctgccgt agagtgtgt 
      tgttgcggg cgcccgaac aaataaata aactatcta 
      tgagacttg agtgtcgtt agccccctt ttgagtgtt 
      tggtgcccc caagtgtcc ccagctggt tttttagtg 
      atttcagtg tcattagga ttgaaaaca aagatcctg 
      tccgaccga acaaaaaca aagactttt atctaatcg 
      aagcggatt atggaggga atccgataa cggcattgt 
      tccagcctt agatcatat cgttttaca ccatggtgc 
      tcatcaccg accgccaaa aaatcaacc tggtgaacc 
      tcctggtga ccgtctgcg tcgtcttca tcgccgtgg 
      acgtctcct cggggagtt tcgccactc gtatcaata 
      aaattagcg agaatttgg acaaagcct tgtcgcaag 
      tcattcacc aaacgccga attacaccg atttgacgg 
      ataatgcca cttatgtca gtgatgcaa gtttcaatc 
      cccgaggaa tggaagggc tgtataatt tcaccaatg 
      ccttcatgg agctgattt tttcaaagg agatttatc 
      cggaaggtt ttattcgaa tcgaaaata accggatcc 
      agatcggag acattgaaa aggagtgga aaagtctct 
      tgaagaaat acgtgggcc ttttggtca tggtcataa 
      ttttggctt tgctcattg tgtttatgc cgttttgtg 
      gcttgtgtt tctgctgct gcaggtgct gcggcaagt 
      gcggggcga ggtcgcagc cctttgaca aaaaacacg 
      acctctgta agaagttcg tgctcgcga cggtgctca 
      ttggcctgg ggacttgtc tcctgtttg gcgtcgttt 
      gtgcatttg taagcaacc agcagatgg aggaggggg 
      ttggcgatt tgtcgcaaa atctgcgca aaggcgtca 
      ccgatatcg acacttatc ttgatacaa ccaaaaagc 
      agatcgaga ctctcctga agaaaaatt acggagagt 
      ttgagtttg tcttgacca aaatgattg ataacagca 
      cggcggccg tttatggcg aattggtga attattcga 
      atgcgattt cgatgactg aggtcgcaa atattgttg 
      aagcacttc ctgcaattg aggaaaaat ttataacgt 
      tgaagtatt cgacgaatt cgttacgtg tctatgctt 
      cacagttga atgacgctg ttaggaaag tcaaaaaag 
      atatgttgc agattttac gatcgtctg actgcgacc 
      gaatcgcca aatgtaaag aaatcaacc aaaatatta 
      gctcattgc aaactaaca tccacttca acagtctcc 
      ccgacataa cggacaaaa tcgccaaac tggacgaaa 
      tcaccaaaa accaaaccc tcatcgacg ccgtcacca 
      aaggcaaga acgccctcg aagacatca aagtcaaaa 
      tcggcgata aagtcaatg cgaccttag acaaagccc 
      acgaaaaaa tccaagaag ccggcgacc aaatccgcc 
      acggcgccg acaacgtca cccaggtca tcaccgaac 
      tcaagacaa aaatcgaca ccaacacca aaccggtcc 
      tcgacacat ccgaacgct acataaaac agtacagtt 
      tttaccgat actatgccg ctttggcga tcagttgct 
      tgctgcttc tgatcacga tcttcatca cgctgggcc 
      tggtttgcg ggatctgcg gcaaacggc ccgaaggct 
      acggggacg actgttgca ataaagggg ccggaagcc 
      aatttctga tctgcgctg tgttgttca tgtttctga 
      cggggtttg tggtcgccg ttgtcgtcc tggcgttgt 
      tcatggtcg gggtcgtga cccagcggg tggtttgtg 
      acccgatgc gcgatcccg ccaactcgc aagtgttcg 
      atttggtcg acagattgg atttctcca actacggca 
      ttcacataa ccgccagtg aggccttgg ggaagtgct 
      atcgcaaca ggagcatct acgaggtgg ccaatctgg 
      ccgataaat tcaacctgg ccgaagtga aaaactact 
      tgaactcgt atgggatta accagactt tggcagagc 
      tggacattg ggtcgctgg atattctgg acgatttta 
      cgcttttgg gcgagaaag acaaggagt tgttggagt 
      cgtttgcag tctccggct ttgccgaaa ttaattttg 
      acgagttca aagatttgt tgagcacga actttacca 
      cggttaatt tgaccgatc tggctaacg aagtggggg 
      gtttgatac aggaattac aaaagtacg agaacgttg 
      atcaattaa ttgaagact tgcaagtca gcaaaatga 
      acattgaaa tgtacgagg ataaaattg tggagccga 
      tgatcaaaa atgcctcgg ctgtgatcg atctggcca 
      aggatatag aaagagatc tcaggttga atcacagct 
      cgtttagtg acgccatgc aagcgttgt taaatgaac 
      tggaacagg ctgagaata ttttaaaaa tcaatggaa 
      ctgatttcc taaatacga ctgtcatcg aattcacca 
      ctggaattg tcaatcttg tccaaactt acatcgacc 
      gagtggtca ctaaaaccg agctagagg tgggtcaat 
      gtgggccct tgagtatag tgatcaatg caacgttaa 
      catcaacct gtgacaaaa tcctaacac cttggaacg 
      gttactggt ttggtctct tctggtcgc tccttctgt 
      tcattccca caatcatag tttcggtta agttggcca 
      cgctgtacc agaaacaca gtccgtatt cgcgcgatc 
      tggtcgaag ccgaatatt tgtatgatg cgtatgtcg 
      atcgggaca acataccgt tgaatagca ggaatggga 
      aggcgaaga agaagaaga agaagcaca agcgttacg 
      aagaccggc cgattccgt cagctggcg gcgacatgg 
      tggcacgtg aatacgcag ccgggtcga accaagacg 
      ccagatatg ccgacatgg cacccaagc aatgggagg 
      actttccgg ccgggggcc ccccgcagt accagaggg 
      cgcccaccg agtacgaga gaccgcccc cgtattatt 
      accccggtg ctggggaaa ccgctcagt agactgact 
      cgccccctg atggagaac tgcacagac tgactcagc 
      attttatct catcgtcgt ttattttct cattcctgt 
      gaagagttg acgatcaaa caaactttg tgattgtat 
      gacagggag caacttttt attttataa ttgtgatat 
      ttgtgactg ccaaagttc aaaagtgtt gccggttgt 
      gggtacaat catgaagtc gagggtcta gcacaattt 
      tgctcaagt gtggtttga aaggtgtac atagatatt 
      ttcataatc gatgtgtta ttgttatct caatttttc 
      gatagttct aattgtata attcgctta aggggcacg 
      atctaaccg attccaaag tactcctaa ctgttgtgc 
      tcattgttt catcatata cagctgtag ccaatttgc 
      catattctt acttgtaca tgtatttat attagcatg 
      ttaataata attccaatg ttta