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Gene information
General information
TC005459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12784644..12804361
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005459
    iBeetle screen iB_00861

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005459 RA
      MVLITDRQK INLVNLLVT VCVVFIAVD VSSGSFATR 
INKISENLD KALSQVIHQ TPNYTDLTD NATYVSDAS 
FNPRGMEGL YNFTNAFME LIFSKEIYP EGFIRIENN 
RIQIGDIEK EWKSLLKKY VGLLVMVII LALLIVFMP 
FCGLCFCCC RCCGKCGAR SQPFDKKHD LCKKFVLAT 
VLIGLGTCL LFGVVCAFV SNQQMEEGV GDLSQNLRK 
GVTDIDTYL DTTKKQIET LLKKNYGEF EFVLTKMID 
NSTAAVYGE LVNYSNAIS MTEVANIVE ALPAIEEKF 
ITLKYSTNS LRVYASQLN DAVRKVKKD MLQILRSSD 
CDRIAKCKE INQNISSLQ TNIHFNSLP DITDKIAKL 
DEITKNQTL IDAVTKGKN ALEDIKVKI GDKVNATLD 
KAHEKIQEA GDQIRHGAD NVTQVITEL KTKIDTNTK 
PVLDTSERY IKQYSFYRY YAALAISCL LLLITIFIT 
LGLVCGICG KRPEGYGDD CCNKGAGSQ FLICAVLFM 
FLTGFVVAV VVLALFMVG VVTQRVVCD PMRDPANSQ 
VFDLVDRLD FSNYGIHIT ASEALGKCY RNRSIYEVA 
NLADKFNLA EVKNYLNSY GINQTLAEL DIGSLDILD 
DFTLLGEKD KELLESFAV SGFAEINFD EFKDLLSTN 
FTTVNLTDL ANEVGGLIQ ELQKYENVD QLIEDLQVS 
KMNIEMYED KIVEPMIKN ASAVIDLAK DIERDLRLN 
HSSFSDAMQ ALLNELEQA ENILKINGT DFLNTTVIE 
FTTGIVNLV QTYIDRVVT KTELEVGQC GPLSIVINA 
TLTSTCDKI LTPWNGYWF GLFWSLLLF IPTIIVSVK 
LATLYQKHS PYSRDLVEA EYLYDAYVD RDNIPLNSR 
NGKAKKKKK KHKRYEDRP IPSAGGDMV AREYAAGSN 
QDARYADMA PKQWEDFPA GGPPQYQRA PTEYERPPP 
YYYPGAGET AQ
    CDS sequence
    • >TC005459 RA
      atggtgctc atcaccgac cgccaaaaa atcaacctg 
gtgaacctc ctggtgacc gtctgcgtc gtcttcatc 
gccgtggac gtctcctcg gggagtttc gccactcgt 
atcaataaa attagcgag aatttggac aaagccttg 
tcgcaagtc attcaccaa acgccgaat tacaccgat 
ttgacggat aatgccact tatgtcagt gatgcaagt 
ttcaatccc cgaggaatg gaagggctg tataatttc 
accaatgcc ttcatggag ctgattttt tcaaaggag 
atttatccg gaaggtttt attcgaatc gaaaataac 
cggatccag atcggagac attgaaaag gagtggaaa 
agtctcttg aagaaatac gtgggcctt ttggtcatg 
gtcataatt ttggctttg ctcattgtg tttatgccg 
ttttgtggc ttgtgtttc tgctgctgc aggtgctgc 
ggcaagtgc ggggcgagg tcgcagccc tttgacaaa 
aaacacgac ctctgtaag aagttcgtg ctcgcgacg 
gtgctcatt ggcctgggg acttgtctc ctgtttggc 
gtcgtttgt gcatttgta agcaaccag cagatggag 
gagggggtt ggcgatttg tcgcaaaat ctgcgcaaa 
ggcgtcacc gatatcgac acttatctt gatacaacc 
aaaaagcag atcgagact ctcctgaag aaaaattac 
ggagagttt gagtttgtc ttgaccaaa atgattgat 
aacagcacg gcggccgtt tatggcgaa ttggtgaat 
tattcgaat gcgatttcg atgactgag gtcgcaaat 
attgttgaa gcacttcct gcaattgag gaaaaattt 
ataacgttg aagtattcg acgaattcg ttacgtgtc 
tatgcttca cagttgaat gacgctgtt aggaaagtc 
aaaaaagat atgttgcag attttacga tcgtctgac 
tgcgaccga atcgccaaa tgtaaagaa atcaaccaa 
aatattagc tcattgcaa actaacatc cacttcaac 
agtctcccc gacataacg gacaaaatc gccaaactg 
gacgaaatc accaaaaac caaaccctc atcgacgcc 
gtcaccaaa ggcaagaac gccctcgaa gacatcaaa 
gtcaaaatc ggcgataaa gtcaatgcg accttagac 
aaagcccac gaaaaaatc caagaagcc ggcgaccaa 
atccgccac ggcgccgac aacgtcacc caggtcatc 
accgaactc aagacaaaa atcgacacc aacaccaaa 
ccggtcctc gacacatcc gaacgctac ataaaacag 
tacagtttt taccgatac tatgccgct ttggcgatc 
agttgcttg ctgcttctg atcacgatc ttcatcacg 
ctgggcctg gtttgcggg atctgcggc aaacggccc 
gaaggctac ggggacgac tgttgcaat aaaggggcc 
ggaagccaa tttctgatc tgcgctgtg ttgttcatg 
tttctgacg gggtttgtg gtcgccgtt gtcgtcctg 
gcgttgttc atggtcggg gtcgtgacc cagcgggtg 
gtttgtgac ccgatgcgc gatcccgcc aactcgcaa 
gtgttcgat ttggtcgac agattggat ttctccaac 
tacggcatt cacataacc gccagtgag gccttgggg 
aagtgctat cgcaacagg agcatctac gaggtggcc 
aatctggcc gataaattc aacctggcc gaagtgaaa 
aactacttg aactcgtat gggattaac cagactttg 
gcagagctg gacattggg tcgctggat attctggac 
gattttacg cttttgggc gagaaagac aaggagttg 
ttggagtcg tttgcagtc tccggcttt gccgaaatt 
aattttgac gagttcaaa gatttgttg agcacgaac 
tttaccacg gttaatttg accgatctg gctaacgaa 
gtggggggt ttgatacag gaattacaa aagtacgag 
aacgttgat caattaatt gaagacttg caagtcagc 
aaaatgaac attgaaatg tacgaggat aaaattgtg 
gagccgatg atcaaaaat gcctcggct gtgatcgat 
ctggccaag gatatagaa agagatctc aggttgaat 
cacagctcg tttagtgac gccatgcaa gcgttgtta 
aatgaactg gaacaggct gagaatatt ttaaaaatc 
aatggaact gatttccta aatacgact gtcatcgaa 
ttcaccact ggaattgtc aatcttgtc caaacttac 
atcgaccga gtggtcact aaaaccgag ctagaggtg 
ggtcaatgt gggcccttg agtatagtg atcaatgca 
acgttaaca tcaacctgt gacaaaatc ctaacacct 
tggaacggt tactggttt ggtctcttc tggtcgctc 
cttctgttc attcccaca atcatagtt tcggttaag 
ttggccacg ctgtaccag aaacacagt ccgtattcg 
cgcgatctg gtcgaagcc gaatatttg tatgatgcg 
tatgtcgat cgggacaac ataccgttg aatagcagg 
aatgggaag gcgaagaag aagaagaag aagcacaag 
cgttacgaa gaccggccg attccgtca gctggcggc 
gacatggtg gcacgtgaa tacgcagcc gggtcgaac 
caagacgcc agatatgcc gacatggca cccaagcaa 
tgggaggac tttccggcc gggggcccc ccgcagtac 
cagagggcg cccaccgag tacgagaga ccgcccccg 
tattattac cccggtgct ggggaaacc gctcagtag
    mRNA sequence
    • >TC005459 RA
      aggagcgag tccgtggga ggctgccgt agagtgtgt 
tgttgcggg cgcccgaac aaataaata aactatcta 
tgagacttg agtgtcgtt agccccctt ttgagtgtt 
tggtgcccc caagtgtcc ccagctggt tttttagtg 
atttcagtg tcattagga ttgaaaaca aagatcctg 
tccgaccga acaaaaaca aagactttt atctaatcg 
aagcggatt atggaggga atccgataa cggcattgt 
tccagcctt agatcatat cgttttaca ccatggtgc 
tcatcaccg accgccaaa aaatcaacc tggtgaacc 
tcctggtga ccgtctgcg tcgtcttca tcgccgtgg 
acgtctcct cggggagtt tcgccactc gtatcaata 
aaattagcg agaatttgg acaaagcct tgtcgcaag 
tcattcacc aaacgccga attacaccg atttgacgg 
ataatgcca cttatgtca gtgatgcaa gtttcaatc 
cccgaggaa tggaagggc tgtataatt tcaccaatg 
ccttcatgg agctgattt tttcaaagg agatttatc 
cggaaggtt ttattcgaa tcgaaaata accggatcc 
agatcggag acattgaaa aggagtgga aaagtctct 
tgaagaaat acgtgggcc ttttggtca tggtcataa 
ttttggctt tgctcattg tgtttatgc cgttttgtg 
gcttgtgtt tctgctgct gcaggtgct gcggcaagt 
gcggggcga ggtcgcagc cctttgaca aaaaacacg 
acctctgta agaagttcg tgctcgcga cggtgctca 
ttggcctgg ggacttgtc tcctgtttg gcgtcgttt 
gtgcatttg taagcaacc agcagatgg aggaggggg 
ttggcgatt tgtcgcaaa atctgcgca aaggcgtca 
ccgatatcg acacttatc ttgatacaa ccaaaaagc 
agatcgaga ctctcctga agaaaaatt acggagagt 
ttgagtttg tcttgacca aaatgattg ataacagca 
cggcggccg tttatggcg aattggtga attattcga 
atgcgattt cgatgactg aggtcgcaa atattgttg 
aagcacttc ctgcaattg aggaaaaat ttataacgt 
tgaagtatt cgacgaatt cgttacgtg tctatgctt 
cacagttga atgacgctg ttaggaaag tcaaaaaag 
atatgttgc agattttac gatcgtctg actgcgacc 
gaatcgcca aatgtaaag aaatcaacc aaaatatta 
gctcattgc aaactaaca tccacttca acagtctcc 
ccgacataa cggacaaaa tcgccaaac tggacgaaa 
tcaccaaaa accaaaccc tcatcgacg ccgtcacca 
aaggcaaga acgccctcg aagacatca aagtcaaaa 
tcggcgata aagtcaatg cgaccttag acaaagccc 
acgaaaaaa tccaagaag ccggcgacc aaatccgcc 
acggcgccg acaacgtca cccaggtca tcaccgaac 
tcaagacaa aaatcgaca ccaacacca aaccggtcc 
tcgacacat ccgaacgct acataaaac agtacagtt 
tttaccgat actatgccg ctttggcga tcagttgct 
tgctgcttc tgatcacga tcttcatca cgctgggcc 
tggtttgcg ggatctgcg gcaaacggc ccgaaggct 
acggggacg actgttgca ataaagggg ccggaagcc 
aatttctga tctgcgctg tgttgttca tgtttctga 
cggggtttg tggtcgccg ttgtcgtcc tggcgttgt 
tcatggtcg gggtcgtga cccagcggg tggtttgtg 
acccgatgc gcgatcccg ccaactcgc aagtgttcg 
atttggtcg acagattgg atttctcca actacggca 
ttcacataa ccgccagtg aggccttgg ggaagtgct 
atcgcaaca ggagcatct acgaggtgg ccaatctgg 
ccgataaat tcaacctgg ccgaagtga aaaactact 
tgaactcgt atgggatta accagactt tggcagagc 
tggacattg ggtcgctgg atattctgg acgatttta 
cgcttttgg gcgagaaag acaaggagt tgttggagt 
cgtttgcag tctccggct ttgccgaaa ttaattttg 
acgagttca aagatttgt tgagcacga actttacca 
cggttaatt tgaccgatc tggctaacg aagtggggg 
gtttgatac aggaattac aaaagtacg agaacgttg 
atcaattaa ttgaagact tgcaagtca gcaaaatga 
acattgaaa tgtacgagg ataaaattg tggagccga 
tgatcaaaa atgcctcgg ctgtgatcg atctggcca 
aggatatag aaagagatc tcaggttga atcacagct 
cgtttagtg acgccatgc aagcgttgt taaatgaac 
tggaacagg ctgagaata ttttaaaaa tcaatggaa 
ctgatttcc taaatacga ctgtcatcg aattcacca 
ctggaattg tcaatcttg tccaaactt acatcgacc 
gagtggtca ctaaaaccg agctagagg tgggtcaat 
gtgggccct tgagtatag tgatcaatg caacgttaa 
catcaacct gtgacaaaa tcctaacac cttggaacg 
gttactggt ttggtctct tctggtcgc tccttctgt 
tcattccca caatcatag tttcggtta agttggcca 
cgctgtacc agaaacaca gtccgtatt cgcgcgatc 
tggtcgaag ccgaatatt tgtatgatg cgtatgtcg 
atcgggaca acataccgt tgaatagca ggaatggga 
aggcgaaga agaagaaga agaagcaca agcgttacg 
aagaccggc cgattccgt cagctggcg gcgacatgg 
tggcacgtg aatacgcag ccgggtcga accaagacg 
ccagatatg ccgacatgg cacccaagc aatgggagg 
actttccgg ccgggggcc ccccgcagt accagaggg 
cgcccaccg agtacgaga gaccgcccc cgtattatt 
accccggtg ctggggaaa ccgctcagt agactgact 
cgccccctg atggagaac tgcacagac tgactcagc 
attttatct catcgtcgt ttattttct cattcctgt 
gaagagttg acgatcaaa caaactttg tgattgtat 
gacagggag caacttttt attttataa ttgtgatat 
ttgtgactg ccaaagttc aaaagtgtt gccggttgt 
gggtacaat catgaagtc gagggtcta gcacaattt 
tgctcaagt gtggtttga aaggtgtac atagatatt 
ttcataatc gatgtgtta ttgttatct caatttttc 
gatagttct aattgtata attcgctta aggggcacg 
atctaaccg attccaaag tactcctaa ctgttgtgc 
tcattgttt catcatata cagctgtag ccaatttgc 
catattctt acttgtaca tgtatttat attagcatg 
ttaataata attccaatg ttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY