| General information | |||
| TC005459 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid57: 1337522..1357239 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005459 | |
| General information | ||||
| iB_00861 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC005459 RA
MVLITDRQK INLVNLLVT VCVVFIAVD VSSGSFATR INKISENLD KALSQVIHQ TPNYTDLTD NATYVSDAS FNPRGMEGL YNFTNAFME LIFSKEIYP EGFIRIENN RIQIGDIEK EWKSLLKKY VGLLVMVII LALLIVFMP FCGLCFCCC RCCGKCGAR SQPFDKKHD LCKKFVLAT VLIGLGTCL LFGVVCAFV SNQQMEEGV GDLSQNLRK GVTDIDTYL DTTKKQIET LLKKNYGEF EFVLTKMID NSTAAVYGE LVNYSNAIS MTEVANIVE ALPAIEEKF ITLKYSTNS LRVYASQLN DAVRKVKKD MLQILRSSD CDRIAKCKE INQNISSLQ TNIHFNSLP DITDKIAKL DEITKNQTL IDAVTKGKN ALEDIKVKI GDKVNATLD KAHEKIQEA GDQIRHGAD NVTQVITEL KTKIDTNTK PVLDTSERY IKQYSFYRY YAALAISCL LLLITIFIT LGLVCGICG KRPEGYGDD CCNKGAGSQ FLICAVLFM FLTGFVVAV VVLALFMVG VVTQRVVCD PMRDPANSQ VFDLVDRLD FSNYGIHIT ASEALGKCY RNRSIYEVA NLADKFNLA EVKNYLNSY GINQTLAEL DIGSLDILD DFTLLGEKD KELLESFAV SGFAEINFD EFKDLLSTN FTTVNLTDL ANEVGGLIQ ELQKYENVD QLIEDLQVS KMNIEMYED KIVEPMIKN ASAVIDLAK DIERDLRLN HSSFSDAMQ ALLNELEQA ENILKINGT DFLNTTVIE FTTGIVNLV QTYIDRVVT KTELEVGQC GPLSIVINA TLTSTCDKI LTPWNGYWF GLFWSLLLF IPTIIVSVK LATLYQKHS PYSRDLVEA EYLYDAYVD RDNIPLNSR NGKAKKKKK KHKRYEDRP IPSAGGDMV AREYAAGSN QDARYADMA PKQWEDFPA GGPPQYQRA PTEYERPPP YYYPGAGET AQ
- >TC005459 RA
atggtgctc atcaccgac cgccaaaaa atcaacctg gtgaacctc ctggtgacc gtctgcgtc gtcttcatc gccgtggac gtctcctcg gggagtttc gccactcgt atcaataaa attagcgag aatttggac aaagccttg tcgcaagtc attcaccaa acgccgaat tacaccgat ttgacggat aatgccact tatgtcagt gatgcaagt ttcaatccc cgaggaatg gaagggctg tataatttc accaatgcc ttcatggag ctgattttt tcaaaggag atttatccg gaaggtttt attcgaatc gaaaataac cggatccag atcggagac attgaaaag gagtggaaa agtctcttg aagaaatac gtgggcctt ttggtcatg gtcataatt ttggctttg ctcattgtg tttatgccg ttttgtggc ttgtgtttc tgctgctgc aggtgctgc ggcaagtgc ggggcgagg tcgcagccc tttgacaaa aaacacgac ctctgtaag aagttcgtg ctcgcgacg gtgctcatt ggcctgggg acttgtctc ctgtttggc gtcgtttgt gcatttgta agcaaccag cagatggag gagggggtt ggcgatttg tcgcaaaat ctgcgcaaa ggcgtcacc gatatcgac acttatctt gatacaacc aaaaagcag atcgagact ctcctgaag aaaaattac ggagagttt gagtttgtc ttgaccaaa atgattgat aacagcacg gcggccgtt tatggcgaa ttggtgaat tattcgaat gcgatttcg atgactgag gtcgcaaat attgttgaa gcacttcct gcaattgag gaaaaattt ataacgttg aagtattcg acgaattcg ttacgtgtc tatgcttca cagttgaat gacgctgtt aggaaagtc aaaaaagat atgttgcag attttacga tcgtctgac tgcgaccga atcgccaaa tgtaaagaa atcaaccaa aatattagc tcattgcaa actaacatc cacttcaac agtctcccc gacataacg gacaaaatc gccaaactg gacgaaatc accaaaaac caaaccctc atcgacgcc gtcaccaaa ggcaagaac gccctcgaa gacatcaaa gtcaaaatc ggcgataaa gtcaatgcg accttagac aaagcccac gaaaaaatc caagaagcc ggcgaccaa atccgccac ggcgccgac aacgtcacc caggtcatc accgaactc aagacaaaa atcgacacc aacaccaaa ccggtcctc gacacatcc gaacgctac ataaaacag tacagtttt taccgatac tatgccgct ttggcgatc agttgcttg ctgcttctg atcacgatc ttcatcacg ctgggcctg gtttgcggg atctgcggc aaacggccc gaaggctac ggggacgac tgttgcaat aaaggggcc ggaagccaa tttctgatc tgcgctgtg ttgttcatg tttctgacg gggtttgtg gtcgccgtt gtcgtcctg gcgttgttc atggtcggg gtcgtgacc cagcgggtg gtttgtgac ccgatgcgc gatcccgcc aactcgcaa gtgttcgat ttggtcgac agattggat ttctccaac tacggcatt cacataacc gccagtgag gccttgggg aagtgctat cgcaacagg agcatctac gaggtggcc aatctggcc gataaattc aacctggcc gaagtgaaa aactacttg aactcgtat gggattaac cagactttg gcagagctg gacattggg tcgctggat attctggac gattttacg cttttgggc gagaaagac aaggagttg ttggagtcg tttgcagtc tccggcttt gccgaaatt aattttgac gagttcaaa gatttgttg agcacgaac tttaccacg gttaatttg accgatctg gctaacgaa gtggggggt ttgatacag gaattacaa aagtacgag aacgttgat caattaatt gaagacttg caagtcagc aaaatgaac attgaaatg tacgaggat aaaattgtg gagccgatg atcaaaaat gcctcggct gtgatcgat ctggccaag gatatagaa agagatctc aggttgaat cacagctcg tttagtgac gccatgcaa gcgttgtta aatgaactg gaacaggct gagaatatt ttaaaaatc aatggaact gatttccta aatacgact gtcatcgaa ttcaccact ggaattgtc aatcttgtc caaacttac atcgaccga gtggtcact aaaaccgag ctagaggtg ggtcaatgt gggcccttg agtatagtg atcaatgca acgttaaca tcaacctgt gacaaaatc ctaacacct tggaacggt tactggttt ggtctcttc tggtcgctc cttctgttc attcccaca atcatagtt tcggttaag ttggccacg ctgtaccag aaacacagt ccgtattcg cgcgatctg gtcgaagcc gaatatttg tatgatgcg tatgtcgat cgggacaac ataccgttg aatagcagg aatgggaag gcgaagaag aagaagaag aagcacaag cgttacgaa gaccggccg attccgtca gctggcggc gacatggtg gcacgtgaa tacgcagcc gggtcgaac caagacgcc agatatgcc gacatggca cccaagcaa tgggaggac tttccggcc gggggcccc ccgcagtac cagagggcg cccaccgag tacgagaga ccgcccccg tattattac cccggtgct ggggaaacc gctcagtag
- >TC005459 RA
aggagcgag tccgtggga ggctgccgt agagtgtgt tgttgcggg cgcccgaac aaataaata aactatcta tgagacttg agtgtcgtt agccccctt ttgagtgtt tggtgcccc caagtgtcc ccagctggt tttttagtg atttcagtg tcattagga ttgaaaaca aagatcctg tccgaccga acaaaaaca aagactttt atctaatcg aagcggatt atggaggga atccgataa cggcattgt tccagcctt agatcatat cgttttaca ccatggtgc tcatcaccg accgccaaa aaatcaacc tggtgaacc tcctggtga ccgtctgcg tcgtcttca tcgccgtgg acgtctcct cggggagtt tcgccactc gtatcaata aaattagcg agaatttgg acaaagcct tgtcgcaag tcattcacc aaacgccga attacaccg atttgacgg ataatgcca cttatgtca gtgatgcaa gtttcaatc cccgaggaa tggaagggc tgtataatt tcaccaatg ccttcatgg agctgattt tttcaaagg agatttatc cggaaggtt ttattcgaa tcgaaaata accggatcc agatcggag acattgaaa aggagtgga aaagtctct tgaagaaat acgtgggcc ttttggtca tggtcataa ttttggctt tgctcattg tgtttatgc cgttttgtg gcttgtgtt tctgctgct gcaggtgct gcggcaagt gcggggcga ggtcgcagc cctttgaca aaaaacacg acctctgta agaagttcg tgctcgcga cggtgctca ttggcctgg ggacttgtc tcctgtttg gcgtcgttt gtgcatttg taagcaacc agcagatgg aggaggggg ttggcgatt tgtcgcaaa atctgcgca aaggcgtca ccgatatcg acacttatc ttgatacaa ccaaaaagc agatcgaga ctctcctga agaaaaatt acggagagt ttgagtttg tcttgacca aaatgattg ataacagca cggcggccg tttatggcg aattggtga attattcga atgcgattt cgatgactg aggtcgcaa atattgttg aagcacttc ctgcaattg aggaaaaat ttataacgt tgaagtatt cgacgaatt cgttacgtg tctatgctt cacagttga atgacgctg ttaggaaag tcaaaaaag atatgttgc agattttac gatcgtctg actgcgacc gaatcgcca aatgtaaag aaatcaacc aaaatatta gctcattgc aaactaaca tccacttca acagtctcc ccgacataa cggacaaaa tcgccaaac tggacgaaa tcaccaaaa accaaaccc tcatcgacg ccgtcacca aaggcaaga acgccctcg aagacatca aagtcaaaa tcggcgata aagtcaatg cgaccttag acaaagccc acgaaaaaa tccaagaag ccggcgacc aaatccgcc acggcgccg acaacgtca cccaggtca tcaccgaac tcaagacaa aaatcgaca ccaacacca aaccggtcc tcgacacat ccgaacgct acataaaac agtacagtt tttaccgat actatgccg ctttggcga tcagttgct tgctgcttc tgatcacga tcttcatca cgctgggcc tggtttgcg ggatctgcg gcaaacggc ccgaaggct acggggacg actgttgca ataaagggg ccggaagcc aatttctga tctgcgctg tgttgttca tgtttctga cggggtttg tggtcgccg ttgtcgtcc tggcgttgt tcatggtcg gggtcgtga cccagcggg tggtttgtg acccgatgc gcgatcccg ccaactcgc aagtgttcg atttggtcg acagattgg atttctcca actacggca ttcacataa ccgccagtg aggccttgg ggaagtgct atcgcaaca ggagcatct acgaggtgg ccaatctgg ccgataaat tcaacctgg ccgaagtga aaaactact tgaactcgt atgggatta accagactt tggcagagc tggacattg ggtcgctgg atattctgg acgatttta cgcttttgg gcgagaaag acaaggagt tgttggagt cgtttgcag tctccggct ttgccgaaa ttaattttg acgagttca aagatttgt tgagcacga actttacca cggttaatt tgaccgatc tggctaacg aagtggggg gtttgatac aggaattac aaaagtacg agaacgttg atcaattaa ttgaagact tgcaagtca gcaaaatga acattgaaa tgtacgagg ataaaattg tggagccga tgatcaaaa atgcctcgg ctgtgatcg atctggcca aggatatag aaagagatc tcaggttga atcacagct cgtttagtg acgccatgc aagcgttgt taaatgaac tggaacagg ctgagaata ttttaaaaa tcaatggaa ctgatttcc taaatacga ctgtcatcg aattcacca ctggaattg tcaatcttg tccaaactt acatcgacc gagtggtca ctaaaaccg agctagagg tgggtcaat gtgggccct tgagtatag tgatcaatg caacgttaa catcaacct gtgacaaaa tcctaacac cttggaacg gttactggt ttggtctct tctggtcgc tccttctgt tcattccca caatcatag tttcggtta agttggcca cgctgtacc agaaacaca gtccgtatt cgcgcgatc tggtcgaag ccgaatatt tgtatgatg cgtatgtcg atcgggaca acataccgt tgaatagca ggaatggga aggcgaaga agaagaaga agaagcaca agcgttacg aagaccggc cgattccgt cagctggcg gcgacatgg tggcacgtg aatacgcag ccgggtcga accaagacg ccagatatg ccgacatgg cacccaagc aatgggagg actttccgg ccgggggcc ccccgcagt accagaggg cgcccaccg agtacgaga gaccgcccc cgtattatt accccggtg ctggggaaa ccgctcagt agactgact cgccccctg atggagaac tgcacagac tgactcagc attttatct catcgtcgt ttattttct cattcctgt gaagagttg acgatcaaa caaactttg tgattgtat gacagggag caacttttt attttataa ttgtgatat ttgtgactg ccaaagttc aaaagtgtt gccggttgt gggtacaat catgaagtc gagggtcta gcacaattt tgctcaagt gtggtttga aaggtgtac atagatatt ttcataatc gatgtgtta ttgttatct caatttttc gatagttct aattgtata attcgctta aggggcacg atctaaccg attccaaag tactcctaa ctgttgtgc tcattgttt catcatata cagctgtag ccaatttgc catattctt acttgtaca tgtatttat attagcatg ttaataata attccaatg ttta