Status
Please wait ...
Gene information
General information
TC005445 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13071061..13075135
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005445
    iBeetle screen iB_00859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    low validity due to technical reasons - should be repeated: lethality not scorable,
    Adult morphology
    low validity due to technical reasons - should be repeated: lethality not scorable,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005445 RA
      MASPPNDKN TAKNEVSVN VDITKTNMQ TPTSKNNQL 
      GEIAIEASE IVSRLEQVA EGTSLADAA YNQPTVFLG 
      LEQKRTEEI IQIIQGLIS VDEDGEPLS LHSLDNVAK 
      LVMVSHSIA AFCSGLERP VMQKLSTRF TTDATRWIS 
      QIFGFLDSS SFYHDDHLE GLVRVTRMM LHYKYPRYL 
      EDGALAFTA SLPTIYSSV ASPLVVVQH LCRQLGLPL 
      ACVRPVPVN THFGSQYTM DVASLQKML ADDVSAGRT 
      PLIVIADAG TPITGHVDN ISRIKELCR LHDAWLHLR 
      GHSLAALAL PNYQRNGHI VPVADSYTL SLGSWLGIA 
      GLPMVTLYR LWESTSNLN RSKQVGATR ESTLPFLAG 
      LNNDHQGRR ILALPLWAA LQGLGRDGV HSRIRDDFL 
      ASERLWAAL DRYRHVRVL SQKPGGESG TYTVSELIS 
      KPANINLLF ESTACCVVF QFTPELSEG EVLTKVPPY 
      YDKLNSWLG QILQLDAPQ VPVDICELE NVGVVLRFC 
      PFENITGQQ PTLEEIQTF VQCLEQQLV ILRATIQHK 
      ETFIKLVSE SPVLKIVDL PDWAGLGGV RYAPEGWEQ 
      LLTDQAKEE LNNLNMALV DSLRSTDSA FSLGEGADG 
      LMCVRFGML TPQSDVEEL LNLVIRVGQ SVEENSRVL 
      DSMGEIVKK GIETATLDL QKENEERLW QEGILRQVP 
      VVGTFVNWW SPKTKESGV RGRSLNLTQ GVVESTENI 
      YKYHMQISG ATPGAKNPP TPQVQTPIG GSHSRSSSH 
      ASSQGVKIE NEREVPKDS SKRKSLSQT ENKVLTP
    CDS sequence
    • >TC005445 RA
      atggcttct ccgccgaat gataaaaac acagcaaaa 
      aacgaggtt tccgtaaat gtagacata acgaaaaca 
      aacatgcaa actccaact agcaaaaac aaccagttg 
      ggcgaaatc gcaatcgaa gcgtccgaa attgttagt 
      aggttagag caggttgcc gaagggaca agcctggcc 
      gatgcggcc tacaaccag ccgacggtg ttcctgggc 
      ctggagcag aagcgcacc gaggaaatc atccagata 
      atccagggc ttgatcagt gtggatgag gacggggag 
      ccgttgagt ctgcacagt ctggacaac gtggccaag 
      ctggtgatg gtttcgcac agcattgcg gcgttttgc 
      agcgggctg gagcgcccc gtcatgcag aagctgagc 
      acgaggttc accacggac gccacgcgc tggatcagc 
      cagattttc ggttttctc gactcgtcg tcgttttat 
      cacgacgac cacctcgag gggctggtc agagtgacc 
      agaatgatg ctacattac aagtacccc cggtatctg 
      gaagacggt gctctggcc tttactgcc tcgctccca 
      actatttac agcagtgtg gcgagtcct ttggtcgta 
      gtccagcac ttgtgtcgc cagttgggg cttccccta 
      gcctgtgtg aggcccgtg ccggtcaat actcacttc 
      ggttcgcag tacacgatg gacgtagcg tcgttacaa 
      aaaatgtta gctgatgat gttagcgcc ggtaggacc 
      cctttaatt gtgattgcc gatgctggg acccctatt 
      acggggcat gttgataat atttcgagg attaaggag 
      ttgtgtcgg ttacatgac gcttggttg catctaagg 
      gggcacagt ttggccgcc ttggcgctg ccaaactac 
      caacgcaat ggccatata gtcccagtt gctgacagt 
      tatacgtta tcactcggt agttggctg gggatcgcc 
      ggcttacca atggtcact ttgtatcgc ctttgggag 
      tcgacctcg aacctcaat cgcagtaaa caagtggga 
      gccacccgt gaaagtacg cttccattt ctggccggt 
      ctaaataac gaccaccaa ggtcgccga atattggcc 
      ttgccctta tgggccgct ttacagggt ctaggtcga 
      gacggggtg cattctagg ataagggat gattttttg 
      gctagtgaa cggctttgg gcggccttg gaccgctat 
      cggcacgtt cgagttttg agccaaaaa ccgggcggt 
      gagagcggc acatacacc gtttcagag ctcatttcc 
      aagcctgct aacataaac ctgcttttc gagtcgacg 
      gcttgctgc gtagtcttc caattcacc cccgagctg 
      agcgagggc gaagtcctc acaaaagtg cctccctat 
      tacgataaa ctcaactcc tggttgggc caaattttg 
      caattggac gccccccaa gtccccgtc gacatatgc 
      gagctcgaa aacgtcggt gtcgtgcta cgtttctgc 
      ccttttgaa aacatcacc ggtcaacaa cccaccctc 
      gaggaaatc caaactttt gtccaatgt ctcgaacaa 
      cagttggta attctccga gcgacaata caacacaag 
      gaaactttc attaaatta gtttcggaa tcgccggtt 
      ttgaaaata gttgatttg cccgactgg gcggggctt 
      ggcggtgtt agatacgcc cccgaaggg tgggagcaa 
      ctgttaacc gatcaggcg aaggaggag ttgaacaat 
      ttgaacatg gcgcttgtt gattcgcta cgatcgacc 
      gattcggcg ttttcattg ggtgagggg gcggacggg 
      ttgatgtgc gtccggttt ggcatgttg acgccccaa 
      tcggacgtc gaggagctg cttaatcta gtgataagg 
      gtgggacaa tcggtggag gaaaattca agagttttg 
      gattcgatg ggcgagatt gtgaaaaag gggattgaa 
      acggccacc cttgacctc cagaaggaa aacgaggag 
      cggttatgg caggaggga attttacgg caggttccc 
      gtagttggg acttttgtt aattggtgg tcgcctaag 
      actaaagag tcgggagta agggggcgg agtttgaat 
      ttgacgcaa ggcgtggtc gaatccacg gaaaatatt 
      tacaagtat cacatgcaa atcagtggg gcgacgcct 
      ggggcgaaa aaccccccg actccacag gtccagact 
      cccattggg gggagtcat tccaggtcg agtagtcac 
      gcctccagt caaggggtt aaaatagaa aatgaacga 
      gaggttcct aaagatagt agtaagagg aaaagtttg 
      tcacagact gaaaataaa gtattgacg ccttaa
    mRNA sequence
    • >TC005445 RA
      gcatgcaaa taagcgaga gcacccgaa ggcgtacgt 
      ctgcaaaat ctgacgttt ccgtacacc tgccacgat 
      ggcttctcc gccgaatga taaaaacac agcaaaaaa 
      cgaggtttc cgtaaatgt agacataac gaaaacaaa 
      catgcaaac tccaactag caaaaacaa ccagttggg 
      cgaaatcgc aatcgaagc gtccgaaat tgttagtag 
      gttagagca ggttgccga agggacaag cctggccga 
      tgcggccta caaccagcc gacggtgtt cctgggcct 
      ggagcagaa gcgcaccga ggaaatcat ccagataat 
      ccagggctt gatcagtgt ggatgagga cggggagcc 
      gttgagtct gcacagtct ggacaacgt ggccaagct 
      ggtgatggt ttcgcacag cattgcggc gttttgcag 
      cgggctgga gcgccccgt catgcagaa gctgagcac 
      gaggttcac cacggacgc cacgcgctg gatcagcca 
      gattttcgg ttttctcga ctcgtcgtc gttttatca 
      cgacgacca cctcgaggg gctggtcag agtgaccag 
      aatgatgct acattacaa gtacccccg gtatctgga 
      agacggtgc tctggcctt tactgcctc gctcccaac 
      tatttacag cagtgtggc gagtccttt ggtcgtagt 
      ccagcactt gtgtcgcca gttggggct tcccctagc 
      ctgtgtgag gcccgtgcc ggtcaatac tcacttcgg 
      ttcgcagta cacgatgga cgtagcgtc gttacaaaa 
      aatgttagc tgatgatgt tagcgccgg taggacccc 
      tttaattgt gattgccga tgctgggac ccctattac 
      ggggcatgt tgataatat ttcgaggat taaggagtt 
      gtgtcggtt acatgacgc ttggttgca tctaagggg 
      gcacagttt ggccgcctt ggcgctgcc aaactacca 
      acgcaatgg ccatatagt cccagttgc tgacagtta 
      tacgttatc actcggtag ttggctggg gatcgccgg 
      cttaccaat ggtcacttt gtatcgcct ttgggagtc 
      gacctcgaa cctcaatcg cagtaaaca agtgggagc 
      cacccgtga aagtacgct tccatttct ggccggtct 
      aaataacga ccaccaagg tcgccgaat attggcctt 
      gcccttatg ggccgcttt acagggtct aggtcgaga 
      cggggtgca ttctaggat aagggatga ttttttggc 
      tagtgaacg gctttgggc ggccttgga ccgctatcg 
      gcacgttcg agttttgag ccaaaaacc gggcggtga 
      gagcggcac atacaccgt ttcagagct catttccaa 
      gcctgctaa cataaacct gcttttcga gtcgacggc 
      ttgctgcgt agtcttcca attcacccc cgagctgag 
      cgagggcga agtcctcac aaaagtgcc tccctatta 
      cgataaact caactcctg gttgggcca aattttgca 
      attggacgc cccccaagt ccccgtcga catatgcga 
      gctcgaaaa cgtcggtgt cgtgctacg tttctgccc 
      ttttgaaaa catcaccgg tcaacaacc caccctcga 
      ggaaatcca aacttttgt ccaatgtct cgaacaaca 
      gttggtaat tctccgagc gacaataca acacaagga 
      aactttcat taaattagt ttcggaatc gccggtttt 
      gaaaatagt tgatttgcc cgactgggc ggggcttgg 
      cggtgttag atacgcccc cgaagggtg ggagcaact 
      gttaaccga tcaggcgaa ggaggagtt gaacaattt 
      gaacatggc gcttgttga ttcgctacg atcgaccga 
      ttcggcgtt ttcattggg tgagggggc ggacgggtt 
      gatgtgcgt ccggtttgg catgttgac gccccaatc 
      ggacgtcga ggagctgct taatctagt gataagggt 
      gggacaatc ggtggagga aaattcaag agttttgga 
      ttcgatggg cgagattgt gaaaaaggg gattgaaac 
      ggccaccct tgacctcca gaaggaaaa cgaggagcg 
      gttatggca ggagggaat tttacggca ggttcccgt 
      agttgggac ttttgttaa ttggtggtc gcctaagac 
      taaagagtc gggagtaag ggggcggag tttgaattt 
      gacgcaagg cgtggtcga atccacgga aaatattta 
      caagtatca catgcaaat cagtggggc gacgcctgg 
      ggcgaaaaa ccccccgac tccacaggt ccagactcc 
      cattggggg gagtcattc caggtcgag tagtcacgc 
      ctccagtca aggggttaa aatagaaaa tgaacgaga 
      ggttcctaa agatagtag taagaggaa aagtttgtc 
      acagactga aaataaagt attgacgcc ttaatttgt 
      tgatttaga gttggcgtt gtgttttgt tttttgtta 
      ttaaataac tctaaattt tttacagta ttatatttt 
      tacctcgtg tacataact tacagctga gcccacggg 
      ctaatgaaa tatgtattt tgtttcaaa gccgtagta 
      atttaagat agtttataa tgtttgagg agtttatta 
      ggcttttta tttgtattg ttccagttt atcttattc 
      taggtaaaa agaattagc ctttattcc gcaatactg 
      tttttatta ctactagat agccttgtt acttgcaca 
      ttgattctg ataatattg taccaaaga tgcttgaca 
      agcgttcat ttttaataa aacaaatta tgtcgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY