Status
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Gene information
General information
TC005426 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13341469..13344016
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005426
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00857

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005426 RA
      MQCTPEEIE QKRRLALEK LAKKRLISP QSHNLQSEK 
      NAAPQSPFR FKDQSQACK HNAKPYDKP VLSFYGKDA 
      VVTGKTSLI KEDRFVVEL SGFSTPAIE IFKTIPTRN 
      YNGKTRNWD FHVRDHDAL IAKLQTLRP NVLVEKLPV 
      FILSCVNKE RTDYSNIDL SGLDPELGG ALMPFQQEG 
      VRFGIDKNG RCLIADDMG LGKTFQALG IANYYKNDW 
      PLLIVTTSS MKNVWEETI HRYLPSVPI FQIQYMTSG 
      KDYIGDSRV LIVSHDMMS RYVDNLLKK QFGVLIIDE 
      SHNLKNFKA KCTKAATAL AKKAKRVVL LSGTPALSR 
      PSELFSQLS LIDDKFFGN FFDYSKRYC DGKSTNFGW 
      DASGKSNLQ ELEIVLNRK FMIRRTKEE VLKSLPNKS 
      QEVVTLDVN LNQFSVEDK ECLNALAAK YASQKKSAE 
      KHAILLTFF SETAKIKIP SVCSYILQV LKSGQKFLV 
      FAHHQIMLN AVCNVLDKK NVKYIRIDG STTSEQRKF 
      FVDKFQLND DCLVAVLSI TAANAGITL TAAQLVLFA 
      ELHWNPSIL SQAESRAHR IGQQNPVII KYLLAPGTA 
      DDSIWPMLQ NKQKILEEV GLSRDSFEN VSITKQETE 
      NYKENLNVS LNRTSANTL DITSYFKDA QNESLEDVF 
      NDDLDSLLC DIDV
    CDS sequence
    • >TC005426 RA
      atgcaatgc acgcctgaa gagatagag cagaagaga 
      cgcctagcg ttggaaaaa ctggccaag aagcgccta 
      atcagcccc caaagtcat aacctccaa agtgaaaaa 
      aatgcagcc ccacaatca ccgttccgc ttcaaagac 
      caaagccag gcgtgtaag cacaacgcg aagccctac 
      gacaagccc gtgttgtct ttttacggt aaagacgct 
      gtcgtaaca ggcaaaaca agcttaata aaagaagac 
      agatttgtg gtggaatta tccgggttt agcacccca 
      gcgattgag atttttaag actatacca acacgtaat 
      tacaatggt aagacacga aattgggat tttcacgtc 
      cgagaccac gacgcgtta attgcgaaa ttacaaacg 
      ttacgaccc aatgttttg gtcgaaaaa ctaccagtt 
      tttatcctc agttgcgta aataaggaa cgaaccgac 
      tattctaac attgacttg agtggtctt gaccccgaa 
      ctggggggc gccttaatg ccgtttcaa caggaggga 
      gtccgtttt gggattgac aaaaatggg aggtgttta 
      attgcggac gatatgggt ttgggaaaa acgttccag 
      gctttaggc attgccaat tattacaaa aacgactgg 
      cctttgctt atcgtaacg actagttca atgaagaac 
      gtttgggaa gaaaccatc catcggtac ctcccaagt 
      gttccgatt tttcaaata cagtacatg acctcaggg 
      aaggactac attggggac agtcgtgtc ttgatcgta 
      agccacgat atgatgtct cgttacgtc gataattta 
      ttgaaaaaa cagttcggt gtcttgatt attgacgaa 
      tcacacaat ttaaaaaat ttcaaagct aagtgcaca 
      aaagccgca actgcatta gcgaaaaaa gccaaacgt 
      gtcgttcta ttgagtggc acccctgcc ttatcacgt 
      ccgagcgaa ctgttctca cagttgtca ctgattgac 
      gataaattt ttcgggaat tttttcgac tatagcaag 
      cggtattgt gacggaaag tcgaccaat ttcggctgg 
      gacgcgtcc ggaaagtca aacttgcag gagcttgaa 
      atcgtgcta aaccgcaaa tttatgatc agacgcacg 
      aaagaagaa gtgttaaaa tcattaccg aacaaatcg 
      caagaagtc gtcactttg gacgttaat ttaaatcag 
      tttagtgtg gaagacaag gaatgttta aacgccttg 
      gctgcgaaa tacgccagt cagaaaaaa agtgcggaa 
      aaacacgca attttgcta acttttttc agcgaaact 
      gcgaaaatt aaaattccg tcggtgtgt tcgtatatt 
      ttgcaagtt ttgaaaagt gggcaaaag tttttggtt 
      tttgcccac catcagata atgttgaat gcggtttgt 
      aatgtgctt gataagaaa aatgttaaa tatattagg 
      atagatggg agtacgacg tcggaacaa aggaaattt 
      tttgtcgat aagttccag ttaaatgat gattgtcta 
      gtggctgtt ttatcaatc actgcagcc aatgctgga 
      attacgttg actgcagcc cagctggtg ttgtttgca 
      gaattgcat tggaatcca agcatttta agccaagcc 
      gaaagtcgg gcccaccga atagggcaa cagaaccca 
      gtcataatc aaatatttg ttagccccc gggactgca 
      gatgactcc atttggcca atgctccaa aataagcaa 
      aaaatcctg gaagaggtc ggactttcg cgcgattct 
      ttcgagaat gtttcaatt acgaaacaa gagactgaa 
      aattataag gagaatctg aacgtcagt ctgaaccga 
      acaagtgcc aacacttta gatattacg agttatttc 
      aaggatgcg caaaacgag agtctcgag gacgttttt 
      aacgatgat ttagacagt cttttatgt gatattgat 
      gtataa
    mRNA sequence
    • >TC005426 RA
      gtgacaaac tcaaaagtt gtttgaaag ttttttatt 
      tttttacaa acagtttaa atagttatt tgataaaat 
      gcaatgcac gcctgaaga gatagagca gaagagacg 
      cctagcgtt ggaaaaact ggccaagaa gcgcctaat 
      cagccccca aagtcataa cctccaaag tgaaaaaaa 
      tgcagcccc acaatcacc gttccgctt caaagacca 
      aagccaggc gtgtaagca caacgcgaa gccctacga 
      caagcccgt gttgtcttt ttacggtaa agacgctgt 
      cgtaacagg caaaacaag cttaataaa agaagacag 
      atttgtggt ggaattatc cgggtttag caccccagc 
      gattgagat ttttaagac tataccaac acgtaatta 
      caatggtaa gacacgaaa ttgggattt tcacgtccg 
      agaccacga cgcgttaat tgcgaaatt acaaacgtt 
      acgacccaa tgttttggt cgaaaaact accagtttt 
      tatcctcag ttgcgtaaa taaggaacg aaccgacta 
      ttctaacat tgacttgag tggtcttga ccccgaact 
      ggggggcgc cttaatgcc gtttcaaca ggagggagt 
      ccgttttgg gattgacaa aaatgggag gtgtttaat 
      tgcggacga tatgggttt gggaaaaac gttccaggc 
      tttaggcat tgccaatta ttacaaaaa cgactggcc 
      tttgcttat cgtaacgac tagttcaat gaagaacgt 
      ttgggaaga aaccatcca tcggtacct cccaagtgt 
      tccgatttt tcaaataca gtacatgac ctcagggaa 
      ggactacat tggggacag tcgtgtctt gatcgtaag 
      ccacgatat gatgtctcg ttacgtcga taatttatt 
      gaaaaaaca gttcggtgt cttgattat tgacgaatc 
      acacaattt aaaaaattt caaagctaa gtgcacaaa 
      agccgcaac tgcattagc gaaaaaagc caaacgtgt 
      cgttctatt gagtggcac ccctgcctt atcacgtcc 
      gagcgaact gttctcaca gttgtcact gattgacga 
      taaattttt cgggaattt tttcgacta tagcaagcg 
      gtattgtga cggaaagtc gaccaattt cggctggga 
      cgcgtccgg aaagtcaaa cttgcagga gcttgaaat 
      cgtgctaaa ccgcaaatt tatgatcag acgcacgaa 
      agaagaagt gttaaaatc attaccgaa caaatcgca 
      agaagtcgt cactttgga cgttaattt aaatcagtt 
      tagtgtgga agacaagga atgtttaaa cgccttggc 
      tgcgaaata cgccagtca gaaaaaaag tgcggaaaa 
      acacgcaat tttgctaac ttttttcag cgaaactgc 
      gaaaattaa aattccgtc ggtgtgttc gtatatttt 
      gcaagtttt gaaaagtgg gcaaaagtt tttggtttt 
      tgcccacca tcagataat gttgaatgc ggtttgtaa 
      tgtgcttga taagaaaaa tgttaaata tattaggat 
      agatgggag tacgacgtc ggaacaaag gaaattttt 
      tgtcgataa gttccagtt aaatgatga ttgtctagt 
      ggctgtttt atcaatcac tgcagccaa tgctggaat 
      tacgttgac tgcagccca gctggtgtt gtttgcaga 
      attgcattg gaatccaag cattttaag ccaagccga 
      aagtcgggc ccaccgaat agggcaaca gaacccagt 
      cataatcaa atatttgtt agcccccgg gactgcaga 
      tgactccat ttggccaat gctccaaaa taagcaaaa 
      aatcctgga agaggtcgg actttcgcg cgattcttt 
      cgagaatgt ttcaattac gaaacaaga gactgaaaa 
      ttataagga gaatctgaa cgtcagtct gaaccgaac 
      aagtgccaa cactttaga tattacgag ttatttcaa 
      ggatgcgca aaacgagag tctcgagga cgtttttaa 
      cgatgattt agacagtct tttatgtga tattgatgt 
      ataattcgt tggttataa aacattttt ccaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY